(Circular) (Six-base) MAPSORT of: pFN21AB5026.seq Check: 3381 from: 1 to: 6049 pFN21AB5026 6049 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3859 278 Size: 53 83 186 936 2323 2468 Fragments arranged by size: 2468 2323 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2544 3392 1937 Size: 607 848 4594 Fragments arranged by size: 4594 848 607 Acc65I G'GTAC_C Cuts at: 3370 3370 Size: 6049 AclI AA'CG_TT Cuts at: 1563 4176 4549 1563 Size: 2613 373 3063 Fragments arranged by size: 3063 2613 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2110 2184 2384 2603 3255 4112 5496 5764 Size: 478 74 200 219 652 857 1384 268 Cuts at: 5764 1632 Size: 1917 Fragments arranged by size: 1917 1384 857 652 478 268 219 200 74 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5852 AflIII A'CryG_T Cuts at: 1203 2776 4948 5925 1203 Size: 1573 2172 977 1327 Fragments arranged by size: 2172 1573 1327 977 AgeI A'CCGG_T Cuts at: 4880 4880 Size: 6049 AhdI GACnn_n'nnGTC Cuts at: 4778 4778 Size: 6049 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6049 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6049 AlwNI CAG_nnn'CTG Cuts at: 2053 2158 3118 3233 3792 5364 2053 Size: 105 960 115 559 1572 2738 Fragments arranged by size: 2738 1572 960 559 115 105 ApaI G_GGCC'C Cuts at: 3345 3345 Size: 6049 ApaLI G'TGCA_C Cuts at: 2102 4106 5262 2102 Size: 2004 1156 2889 Fragments arranged by size: 2889 2004 1156 ApoI r'AATT_y Cuts at: 1493 2646 2729 2932 3006 3358 3584 3736 5845 Size: 1153 83 203 74 352 226 152 2109 Cuts at: 5845 1493 Size: 1697 Fragments arranged by size: 2109 1697 1153 352 226 203 152 83 74 AseI AT'TA_AT Cuts at: 160 1032 4603 160 Size: 872 3571 1606 Fragments arranged by size: 3571 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 AvaI C'yCGr_G Cuts at: 1870 1943 2376 2563 3374 1870 Size: 73 433 187 811 4545 Fragments arranged by size: 4545 811 433 187 73 BamHI G'GATC_C Cuts at: 3379 3746 3379 Size: 367 5682 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2974 3370 4825 618 Size: 325 217 627 1187 396 1455 1842 Fragments arranged by size: 1842 1455 1187 627 396 325 217 BanII G_rGCy'C Cuts at: 1391 3345 3368 1391 Size: 1954 23 4072 Fragments arranged by size: 4072 1954 23 BbeI G_GCGC'C Cuts at: 2978 2978 Size: 6049 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2439 928 Size: 971 540 4538 Fragments arranged by size: 4538 971 540 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4240 4274 1903 Size: 34 50 34 2219 34 3678 Fragments arranged by size: 3678 2219 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3941 5151 3941 Size: 1210 4839 BclI T'GATC_A Cuts at: 1548 1548 Size: 6049 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4660 136 Size: 107 122 71 631 605 2988 1525 Fragments arranged by size: 2988 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6044 6044 Size: 6049 BlpI GC'TnA_GC Cuts at: 3466 3466 Size: 6049 Bme1580I G_kGCm'C Cuts at: 1790 2106 3345 4110 5266 1790 Size: 316 1239 765 1156 2573 Fragments arranged by size: 2573 1239 1156 765 316 BmgBI CAC'GTC Cuts at: 1318 2854 1318 Size: 1536 4513 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3780 4738 442 Size: 416 53 83 399 24 2363 958 1753 Fragments arranged by size: 2363 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2018 1056 Size: 962 5087 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3043 3095 3338 4709 1239 Size: 1804 52 243 1371 2579 Fragments arranged by size: 2579 1804 1371 243 52 Bpu10I CC'TnA_GC Cuts at: 3028 3028 Size: 6049 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4174 5039 5337 4174 Size: 865 298 4886 Fragments arranged by size: 4886 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4712 882 Size: 617 3213 2219 Fragments arranged by size: 3213 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3840 493 Size: 711 2636 2702 Fragments arranged by size: 2702 2636 711 BsaBI GATnn'nnATC Cuts at: 2205 6043 2205 Size: 3838 2211 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2975 3856 4238 4873 Size: 53 83 186 936 1442 881 382 635 Cuts at: 4873 275 Size: 1451 Fragments arranged by size: 1451 1442 936 881 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4482 4880 5154 5301 5712 4482 Size: 398 274 147 411 4819 Fragments arranged by size: 4819 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2526 2556 1178 Size: 30 165 30 1123 30 4671 Fragments arranged by size: 4671 1123 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3335 1186 Size: 2149 3900 BseYI C'CCAG_C Cuts at: 2404 5252 5748 2404 Size: 2848 496 2705 Fragments arranged by size: 2848 2705 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3284 1163 Size: 573 1548 3928 Fragments arranged by size: 3928 1548 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3499 4260 5288 1540 Size: 275 186 1498 761 1028 2301 Fragments arranged by size: 2301 1498 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2106 4110 4195 5266 2106 Size: 2004 85 1071 2889 Fragments arranged by size: 2889 2004 1071 85 BsmI GAATG_Cn' Cuts at: 3560 3653 3560 Size: 93 5956 BsmBI CGTCTCn'nnnn_ Cuts at: 2844 2844 Size: 6049 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 3345 3368 4110 4195 5266 1391 Size: 399 316 1239 23 742 85 1071 2174 Fragments arranged by size: 2174 1239 1071 742 399 316 85 23 BspEI T'CCGG_A Cuts at: 5712 5712 Size: 6049 BspHI T'CATG_A Cuts at: 3938 5668 3938 Size: 1730 4319 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3404 844 Size: 2560 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3936 1394 Size: 429 2113 3507 Fragments arranged by size: 3507 2113 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2250 4544 4718 65 Size: 1183 1002 2294 174 1396 Fragments arranged by size: 2294 1396 1183 1002 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4693 4880 1700 Size: 110 2883 187 2869 Fragments arranged by size: 2883 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2723 3181 96 Size: 2627 458 2964 Fragments arranged by size: 2964 2627 458 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6049 BssSI C'ACGA_G Cuts at: 4109 5121 4109 Size: 1012 5037 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6049 BstBI TT'CG_AA Cuts at: 4853 4853 Size: 6049 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6049 BstXI CCAn_nnnn'nTGG Cuts at: 2272 2272 Size: 6049 BstYI r'GATC_y Cuts at: 1972 2070 2744 3095 3379 3746 4131 4148 5600 Size: 98 674 351 284 367 385 17 1452 Cuts at: 5600 5686 5698 6044 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 674 385 367 351 346 284 98 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2037 3288 513 Size: 552 972 1251 3274 Fragments arranged by size: 3274 1251 972 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5310 Fragments arranged by size: 5310 683 56 BtsI GCAGTG_nn' Cuts at: 3128 3565 4359 4379 3128 Size: 437 794 20 4798 Fragments arranged by size: 4798 794 437 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6049 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6014 DraI TTT'AAA Cuts at: 2835 3353 2835 Size: 518 5531 DraIII CAC_nnn'GTG Cuts at: 3198 3198 Size: 6049 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5056 809 Size: 507 3740 1802 Fragments arranged by size: 3740 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2479 3496 4385 Size: 54 1425 50 275 18 649 1017 889 Cuts at: 4385 6014 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1017 889 649 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3496 1537 Size: 275 1684 4090 Fragments arranged by size: 4090 1684 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2129 3979 1353 Size: 776 1850 3423 Fragments arranged by size: 3423 1850 776 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3343 4636 5010 5156 387 Size: 1064 138 1754 1293 374 146 1280 Fragments arranged by size: 1754 1293 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2110 2184 2384 2603 3043 3095 3255 Size: 393 478 74 200 219 440 52 160 Cuts at: 3255 3338 4112 4709 5496 5764 1239 Size: 83 774 597 787 268 1524 Fragments arranged by size: 1524 787 774 597 478 440 393 268 219 200 160 83 74 52 EcoO109I rG'GnC_Cy Cuts at: 3341 3342 3341 Size: 1 6048 EcoRI G'AATT_C Cuts at: 2729 2932 3006 3358 3736 2729 Size: 203 74 352 378 5042 Fragments arranged by size: 5042 378 352 203 74 EcoRV GAT'ATC Cuts at: 2507 2507 Size: 6049 FspI TGC'GCA Cuts at: 4555 4555 Size: 6049 HaeII r_GCGC'y Cuts at: 1678 2978 3317 5196 1678 Size: 1300 339 1879 2531 Fragments arranged by size: 2531 1879 1300 339 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2526 2558 2729 2761 4690 Size: 32 135 32 954 32 171 32 1929 Cuts at: 4690 4722 4764 4796 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1929 954 171 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3393 3639 669 Size: 988 281 1455 246 3079 Fragments arranged by size: 3079 1455 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6049 HpaI GTT'AAC Cuts at: 3639 3639 Size: 6049 KasI G'GCGC_C Cuts at: 2974 2974 Size: 6049 KpnI G_GTAC'C Cuts at: 3374 3374 Size: 6049 MfeI C'AATT_G Cuts at: 3648 3648 Size: 6049 MluI A'CGCG_T Cuts at: 5925 5925 Size: 6049 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2935 3139 5163 5347 1305 Size: 1630 204 2024 184 2007 Fragments arranged by size: 2024 2007 1630 204 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2481 10 Size: 54 1425 343 649 3578 Fragments arranged by size: 3578 1425 649 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2270 3015 3302 4007 4366 4525 518 Size: 648 1104 745 287 705 359 159 2042 Fragments arranged by size: 2042 1104 745 705 648 359 287 159 MspA1I CmG'CkG Cuts at: 1606 2926 3465 4142 5290 5535 1606 Size: 1320 539 677 1148 245 2120 Fragments arranged by size: 2120 1320 1148 677 539 245 NarI GG'CG_CC Cuts at: 2975 2975 Size: 6049 NcoI C'CATG_G Cuts at: 513 1065 2037 513 Size: 552 972 4525 Fragments arranged by size: 4525 972 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6049 NheI G'CTAG_C Cuts at: 1052 2014 1052 Size: 962 5087 NotI GC'GGCC_GC Cuts at: 3496 3496 Size: 6049 NspI r_CATG'y Cuts at: 1132 1229 2780 3407 4952 1132 Size: 97 1551 627 1545 2229 Fragments arranged by size: 2229 1551 1545 627 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6049 PciI A'CATG_T Cuts at: 2776 4948 2776 Size: 2172 3877 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2053 2081 1108 Size: 902 43 28 5076 Fragments arranged by size: 5076 902 43 28 PmeI GTTT'AAAC Cuts at: 3353 3353 Size: 6049 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4118 4150 5631 5663 4118 Size: 32 1481 32 4504 Fragments arranged by size: 4504 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6049 PsiI TTA'TAA Cuts at: 2655 3619 2655 Size: 964 5085 PspOMI G'GGCC_C Cuts at: 3341 3341 Size: 6049 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2337 2369 2484 2516 2337 Size: 32 115 32 5870 Fragments arranged by size: 5870 115 32 32 PstI C_TGCA'G Cuts at: 830 2313 3123 3401 830 Size: 1483 810 278 3478 Fragments arranged by size: 3478 1483 810 278 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6049 PvuII CAG'CTG Cuts at: 2926 2926 Size: 6049 SalI G'TCGA_C Cuts at: 1936 3391 1936 Size: 1455 4594 SbfI CC_TGCA'GG Cuts at: 3401 3401 Size: 6049 ScaI AGT'ACT Cuts at: 4297 6009 4297 Size: 1712 4337 SexAI A'CCwGG_T Cuts at: 2046 2046 Size: 6049 SfcI C'TryA_G Cuts at: 826 1045 2175 2309 2937 3119 3397 4532 5213 Size: 219 1130 134 628 182 278 1135 681 Cuts at: 5213 5404 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1130 681 628 278 219 191 182 134 SfoI GGC'GCC Cuts at: 2976 2976 Size: 6049 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6049 SmaI CCC'GGG Cuts at: 1872 3376 1872 Size: 1504 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4153 5054 5316 820 Size: 197 926 2210 901 262 1553 Fragments arranged by size: 2210 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3840 493 Size: 3347 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6049 SphI G_CATG'C Cuts at: 1132 3407 1132 Size: 2275 3774 SspI AAT'ATT Cuts at: 5 52 2577 3973 5 Size: 47 2525 1396 2081 Fragments arranged by size: 2525 2081 1396 47 StuI AGG'CCT Cuts at: 1517 2814 1517 Size: 1297 4752 StyI C'CwwG_G Cuts at: 513 1065 2020 2037 2782 3488 513 Size: 552 955 17 745 706 3074 Fragments arranged by size: 3074 955 745 706 552 17 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3292 4076 4093 4246 4431 4876 325 Size: 1255 1712 784 17 153 185 445 1498 Fragments arranged by size: 1712 1498 1255 784 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2723 3181 4295 Size: 274 80 33 51 1443 746 458 1114 Cuts at: 4295 6007 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1114 746 458 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6009 6041 6009 Size: 32 6017 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6049 XbaI T'CTAG_A Cuts at: 3385 3385 Size: 6049 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6049 XmaI C'CCGG_G Cuts at: 1870 3374 1870 Size: 1504 4545 XmnI GAAnn'nnTTC Cuts at: 3010 4178 3010 Size: 1168 4881 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3857 276 Size: 53 83 186 936 2323 2468 Fragments arranged by size: 2468 2323 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI