Restriction map for
pFN21AB4980
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# NO CUT ENZYMES ( 106 enzymes) #
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AatII Acc65I AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AviII AvrII BbeI BbrPI
BcgI BciVI BfrI BlnI BsaAI
BsaBI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tsp509I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AccI 58 GTCGAC GT^[AC][GT]AC
AcyI 136 GACGCC G[GA]^CG[CT]C
AflIII 2138 ACGTGT A^C[GA][CT]GT
ApaI 908 GGGCCC GGGCC^C
Asp700I 1437 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 175 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 758 CCCGGG C^[CT]CG[GA]G
BamHI 599 GGATCC G^GATCC
BclI 1610 TGATCA T^GATCA
BglI 1736 GCCAGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1439 AGATCT A^GATCT
BsaHI 136 GACGCC G[GA]^CG[CT]C
BsmI 1049 GCATTC GAATGC(1/-1)
BsoBI 758 CCCGGG C^[CT]CG[GA]G
BsrDI 1716 CATTGC GCAATG(2/0)
EcoNI 450 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1610 TGATCA T^GATCA
HgaI 136 GACGC GACGC(5/10)
Hin1I 136 GACGCC G[GA]^CG[CT]C
HindIII 2410 AAGCTT A^AGCTT
MseI 389 TTAA T^TAA
NaeI 776 GCCGGC GCC^GGC
NgoMIV 776 GCCGGC G^CCGGC
PflFI 175 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 1978 GAGCTC GAGCT^C
SalI 58 GTCGAC G^TCGAC
SapI 1158 GCTCTTC GCTCTTC(1/4)
SmaI 758 CCCGGG CCC^GGG
Tru9I 389 TTAA T^TAA
Tth111I 175 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 758 CCCGGG C^CCGGG
XmaI 758 CCCGGG C^CCGGG
XmnI 1437 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccII 962 CGCG CG^CG
1309 CGCG
AccIII 1586 TCCGGA T^CCGGA
1874 TCCGGA
BbvCI 463 GCTGAGG CCTCAGC(-5/-2)
1381 GCTGAGG
BlpI 1327 GCTGAGC GC^T[ACGT]AGC
1828 GCTCAGC
Bpu1102I 1327 GCTGAGC GC^T[ACGT]AGC
1828 GCTCAGC
BsaI 553 GAGACC GGTCTC(1/5)
18 GGTCTC
BsaWI 1586 TCCGGA [AT]^CCGG[AT]
1874 TCCGGA
BseAI 1586 TCCGGA T^CCGGA
1874 TCCGGA
BsgI 484 CTGCAC GTGCAG(16/14)
43 GTGCAG
BspEI 1586 TCCGGA T^CCGGA
1874 TCCGGA
BsrBI 1114 GAGCGG CCGCTC(-3/-3)
1330 GAGCGG
BsrFI 776 GCCGGC [GA]^CCGG[CT]
1604 ACCGGC
BstUI 962 CGCG CG^CG
1309 CGCG
CelII 1327 GCTGAGC GC^T[ACGT]AGC
1828 GCTCAGC
Cfr10I 776 GCCGGC [GA]^CCGG[CT]
1604 ACCGGC
FauI 963 GCGGG CCCGC(4/6)
1332 GCGGG
HincII 58 GTCGAC GT[CT]^[GA]AC
2134 GTCAAC
HindII 58 GTCGAC GT[CT]^[GA]AC
2134 GTCAAC
MaeII 1637 ACGT A^CGT
2138 ACGT
MroI 1586 TCCGGA T^CCGGA
1874 TCCGGA
MslI 1935 CATCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2037 CATGAGCATG
MvnI 962 CGCG CG^CG
1309 CGCG
NspI 2042 GCATGC [GA]CATG^[CT]
2253 GCATGC
PvuII 625 CAGCTG CAG^CTG
2310 CAGCTG
SphI 2042 GCATGC GCATG^C
2253 GCATGC
TaqI 59 TCGA T^CGA
879 TCGA
TthHB8I 59 TCGA T^CGA
879 TCGA
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AspHI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI TseI Tsp45I TspRI
XcmI XhoII