Restriction map for
pFN21AB4980
(Linear) (Six-base) MAPSORT of: KIBB4980.seq Check: 7086 from: 1 to: 2541
KIBB4980.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 83 2174 2541
Size: 83 2091 367
Fragments arranged by size:
2091 367 83
AccI GT'mk_AC
Cuts at: 0 59 2541
Size: 59 2482
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 298 613 868 1390 1671 2541
Size: 175 123 315 255 522 281 870
Fragments arranged by size:
870 522 315 281 255 175 123
AflIII A'CryG_T
Cuts at: 0 2138 2541
Size: 2138 403
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 247 2541
Size: 247 2294
AlwNI CAG_nnn'CTG
Cuts at: 0 178 627 846 1215 2229 2541
Size: 178 449 219 369 1014 312
Fragments arranged by size:
1014 449 369 312 219 178
ApaI G_GGCC'C
Cuts at: 0 912 2541
Size: 912 1629
AvaI C'yCGr_G
Cuts at: 0 758 2541
Size: 758 1783
BamHI G'GATC_C
Cuts at: 0 599 2541
Size: 599 1942
BanI G'GyrC_C
Cuts at: 0 1335 1998 2064 2541
Size: 1335 663 66 477
Fragments arranged by size:
1335 663 477 66
BanII G_rGCy'C
Cuts at: 0 752 912 1664 1982 2541
Size: 752 160 752 318 559
Fragments arranged by size:
752 752 559 318 160
BbsI GAAGACnn'nnnn_
Cuts at: 0 856 2312 2369 2541
Size: 856 1456 57 172
Fragments arranged by size:
1456 856 172 57
BbvCI CC'TCA_GC
Cuts at: 0 464 1382 2541
Size: 464 918 1159
Fragments arranged by size:
1159 918 464
BclI T'GATC_A
Cuts at: 0 1610 2541
Size: 1610 931
BglI GCCn_nnn'nGGC
Cuts at: 0 1742 2541
Size: 1742 799
BglII A'GATC_T
Cuts at: 0 1439 2541
Size: 1439 1102
BlpI GC'TnA_GC
Cuts at: 0 1328 1829 2541
Size: 1328 501 712
Fragments arranged by size:
1328 712 501
Bme1580I G_kGCm'C
Cuts at: 0 165 912 1037 1126 1316 1340 2069 2350
Size: 165 747 125 89 190 24 729 281
Cuts at: 2350 2460 2541
Size: 110 81
Fragments arranged by size:
747 729 281 190 165 125 110 89
81 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 527 1813 2528 2541
Size: 527 1286 715 13
Fragments arranged by size:
1286 715 527 13
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 995 2541
Size: 995 1546
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 581 655 893 1016 1031 1192 1966
Size: 121 460 74 238 123 15 161 774
Cuts at: 1966 2440 2541
Size: 474 101
Fragments arranged by size:
774 474 460 238 161 123 121 101
74 15
Bpu10I CC'TnA_GC
Cuts at: 0 464 1382 2317 2541
Size: 464 918 935 224
Fragments arranged by size:
935 918 464 224
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 271 367 766 915 1078 2541
Size: 271 96 399 149 163 1463
Fragments arranged by size:
1463 399 271 163 149 96
BsaI GGTCTCn'nnnn_
Cuts at: 0 24 547 2541
Size: 24 523 1994
Fragments arranged by size:
1994 523 24
BsaHI Gr'CG_yC
Cuts at: 0 137 2541
Size: 137 2404
BsaWI w'CCGG_w
Cuts at: 0 1586 1874 2541
Size: 1586 288 667
Fragments arranged by size:
1586 667 288
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1336 1366 1568 1598 2077 2107 2450 2480
Size: 1336 30 202 30 479 30 343 30
Cuts at: 2480 2541
Size: 61
Fragments arranged by size:
1336 479 343 202 61 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 667 1156 1669 2366 2541
Size: 667 489 513 697 175
Fragments arranged by size:
697 667 513 489 175
BseYI C'CCAG_C
Cuts at: 0 707 912 1521 1926 2179 2541
Size: 707 205 609 405 253 362
Fragments arranged by size:
707 609 405 362 253 205
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 469 2541
Size: 64 405 2072
Fragments arranged by size:
2072 405 64
BsiHKAI G_wGCw'C
Cuts at: 0 226 1160 1418 1982 2541
Size: 226 934 258 564 559
Fragments arranged by size:
934 564 559 258 226
BsmI GAATG_Cn'
Cuts at: 0 1049 2541
Size: 1049 1492
Bsp1286I G_dGCh'C
Cuts at: 0 165 226 752 912 1037 1126 1160 1316
Size: 165 61 526 160 125 89 34 156
Cuts at: 1316 1340 1418 1664 1982 2069 2350 2460 2541
Size: 24 78 246 318 87 281 110 81
Fragments arranged by size:
526 318 281 246 165 160 156 125
110 89 87 81 78 61 34 24
BspEI T'CCGG_A
Cuts at: 0 1586 1874 2541
Size: 1586 288 667
Fragments arranged by size:
1586 667 288
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 83 1094 2174 2541
Size: 83 1011 1080 367
Fragments arranged by size:
1080 1011 367 83
BsrBI CCG'CTC
Cuts at: 0 1116 1332 2541
Size: 1116 216 1209
Fragments arranged by size:
1209 1116 216
BsrDI GCAATG_nn'
Cuts at: 0 1715 2541
Size: 1715 826
BsrFI r'CCGG_y
Cuts at: 0 776 1604 2541
Size: 776 828 937
Fragments arranged by size:
937 828 776
BstYI r'GATC_y
Cuts at: 0 428 599 1439 1514 1821 2498 2541
Size: 428 171 840 75 307 677 43
Fragments arranged by size:
840 677 428 307 171 75 43
Bsu36I CC'TnA_GG
Cuts at: 0 980 2246 2342 2521 2541
Size: 980 1266 96 179 20
Fragments arranged by size:
1266 980 179 96 20
BtgI C'CryG_G
Cuts at: 0 1242 1527 1946 1994 2297 2541
Size: 1242 285 419 48 303 244
Fragments arranged by size:
1242 419 303 285 244 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 749 2541
Size: 749 1792
BtsI GCAGTG_nn'
Cuts at: 0 1552 2541
Size: 1552 989
EaeI y'GGCC_r
Cuts at: 0 287 471 536 774 2541
Size: 287 184 65 238 1767
Fragments arranged by size:
1767 287 238 184 65
EarI CTCTTCn'nnn_
Cuts at: 0 589 1165 1429 2541
Size: 589 576 264 1112
Fragments arranged by size:
1112 589 576 264
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 331 2541
Size: 331 2210
EcoICRI GAG'CTC
Cuts at: 0 1980 2541
Size: 1980 561
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 175 298 581 613 655 868 893
Size: 121 54 123 283 32 42 213 25
Cuts at: 893 1016 1031 1192 1390 1671 1966 2440 2541
Size: 123 15 161 198 281 295 474 101
Fragments arranged by size:
474 295 283 281 213 198 161 123
123 121 101 54 42 32 25 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 454 2541
Size: 454 2087
EcoO109I rG'GnC_Cy
Cuts at: 0 270 531 908 909 985 1212 2226 2400
Size: 270 261 377 1 76 227 1014 174
Cuts at: 2400 2447 2541
Size: 47 94
Fragments arranged by size:
1014 377 270 261 227 174 94 76
47 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 414 2541
Size: 414 2127
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 246 278 486 518 1004 1036 2541
Size: 246 32 208 32 486 32 1505
Fragments arranged by size:
1505 486 246 208 32 32 32
HincII GTy'rAC
Cuts at: 0 60 2136 2541
Size: 60 2076 405
Fragments arranged by size:
2076 405 60
HindIII A'AGCT_T
Cuts at: 0 2410 2541
Size: 2410 131
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 2541
Size: 158 2383
MscI TGG'CCA
Cuts at: 0 289 473 538 2541
Size: 289 184 65 2003
Fragments arranged by size:
2003 289 184 65
MslI CAynn'nnrTG
Cuts at: 0 1939 2041 2541
Size: 1939 102 500
Fragments arranged by size:
1939 500 102
MspA1I CmG'CkG
Cuts at: 0 48 627 927 2312 2541
Size: 48 579 300 1385 229
Fragments arranged by size:
1385 579 300 229 48
NaeI GCC'GGC
Cuts at: 0 778 2541
Size: 778 1763
NcoI C'CATG_G
Cuts at: 0 1242 1994 2297 2541
Size: 1242 752 303 244
Fragments arranged by size:
1242 752 303 244
NgoMIV G'CCGG_C
Cuts at: 0 776 2541
Size: 776 1765
NspI r_CATG'y
Cuts at: 0 2046 2257 2541
Size: 2046 211 284
Fragments arranged by size:
2046 284 211
PasI CC'CwG_GG
Cuts at: 0 113 1340 2223 2541
Size: 113 1227 883 318
Fragments arranged by size:
1227 883 318 113
PfoI T'CCnGG_A
Cuts at: 0 671 1562 1817 2153 2417 2541
Size: 671 891 255 336 264 124
Fragments arranged by size:
891 671 336 264 255 124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1051 1083 2387 2419 2541
Size: 1051 32 1304 32 122
Fragments arranged by size:
1304 1051 122 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 270 985 2400 2447 2541
Size: 270 715 1415 47 94
Fragments arranged by size:
1415 715 270 94 47
PspOMI G'GGCC_C
Cuts at: 0 908 2541
Size: 908 1633
PstI C_TGCA'G
Cuts at: 0 413 455 551 2171 2474 2541
Size: 413 42 96 1620 303 67
Fragments arranged by size:
1620 413 303 96 67 42
PvuII CAG'CTG
Cuts at: 0 627 2312 2541
Size: 627 1685 229
Fragments arranged by size:
1685 627 229
SacI G_AGCT'C
Cuts at: 0 1982 2541
Size: 1982 559
SalI G'TCGA_C
Cuts at: 0 58 2541
Size: 58 2483
SanDI GG'GwC_CC
Cuts at: 0 270 2541
Size: 270 2271
SapI GCTCTTCn'nnn_
Cuts at: 0 1165 2541
Size: 1165 1376
SfcI C'TryA_G
Cuts at: 0 409 451 547 712 2167 2470 2541
Size: 409 42 96 165 1455 303 71
Fragments arranged by size:
1455 409 303 165 96 71 42
SmaI CCC'GGG
Cuts at: 0 760 2541
Size: 760 1781
SmlI C'TyrA_G
Cuts at: 0 250 382 745 894 1093 2541
Size: 250 132 363 149 199 1448
Fragments arranged by size:
1448 363 250 199 149 132
SphI G_CATG'C
Cuts at: 0 2046 2257 2541
Size: 2046 211 284
Fragments arranged by size:
2046 284 211
StuI AGG'CCT
Cuts at: 0 196 1471 2114 2541
Size: 196 1275 643 427
Fragments arranged by size:
1275 643 427 196
StyI C'CwwG_G
Cuts at: 0 1037 1242 1859 1994 2013 2297 2450 2541
Size: 1037 205 617 135 19 284 153 91
Fragments arranged by size:
1037 617 284 205 153 135 91 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 79 1826 2541
Size: 79 1747 715
Fragments arranged by size:
1747 715 79
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1336 1368 2077 2109 2450 2482 2541
Size: 1336 32 709 32 341 32 59
Fragments arranged by size:
1336 709 341 59 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 178 2541
Size: 178 2363
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 816 924 1800 2541
Size: 816 108 876 741
Fragments arranged by size:
876 816 741 108
XmaI C'CCGG_G
Cuts at: 0 758 2541
Size: 758 1783
XmnI GAAnn'nnTTC
Cuts at: 0 1441 2541
Size: 1441 1100
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AlwNI ApaI AvaI
BamHI BanI BanII BbsI BbvCI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstYI Bsu36I
BtgI BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI Hin4I HincII HindIII MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PasI PfoI
PpiI PpuMI PspOMI PstI PvuII SacI SalI SanDI
SapI SfcI SmaI SmlI SphI StuI StyI TaqII
TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbeI BcgI BciVI BfrBI BmgBI BmtI BsaAI BsaBI
BsiEI BsiWI BsmBI BspHI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I ClaI CspCI DraI DraIII
DrdI EagI EcoRI EcoRV FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI NarI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI TatI XbaI XhoI ZraI