Restriction map for
pFN21AB4964
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# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AccIII AflII Aor51HI AscI
AseI AsnI Asp718I AspI AviII
BbvCI BfrI BsaBI BseAI BsgI
BsiWI BspEI BsrDI BssHII Bst1107I
BstZ17I EagI EclXI Eco47III Eco52I
EcoRV FseI FspI KpnI KspI
MamI MroI NdeI NotI NruI
PacI PaeR7I PflFI PmeI PshBI
PvuI SacII SbfI ScaI SfiI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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AatII 420 GACGTC GACGT^C
AclI 1550 AACGTT AA^CGTT
AgeI 2275 ACCGGT A^CCGGT
AocI 1152 CCTGAGG CC^T[ACGT]AGG
ApaI 165 GGGCCC GGGCC^C
Asp700I 3949 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 2900 TGGCCA TGG^CCA
BbeI 63 GGCGCC GGCGC^C
BcgI 1522 GCAGTTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1747 GGATAC GTATCC(6/5)
BglII 3347 AGATCT A^GATCT
BlpI 1146 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1146 GCTGAGC GC^T[ACGT]AGC
Bsp1407I 4358 TGTACA T^GTACA
BspDI 3742 ATCGAT AT^CGAT
BspLU11I 2678 ACATGT A^CATGT
BspMI 3792 GCAGGT ACCTGC(4/8)
BsrBI 729 CCGCTC CCGCTC(-3/-3)
BsrGI 4358 TGTACA T^GTACA
BssSI 292 CTCGTG CACGAG(-5/-1)
BstBI 152 TTCGAA TT^CGAA
BstXI 1076 CCAGGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1152 CCTGAGG CC^T[ACGT]AGG
CelII 1146 GCTGAGC GC^T[ACGT]AGC
ClaI 3742 ATCGAT AT^CGAT
CpoI 57 CGGTCCG CG^G[AT]CCG
Eco81I 1152 CCTGAGG CC^T[ACGT]AGG
EcoNI 1230 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 544 GAATTC G^AATTC
EcoT22I 2101 ATGCAT ATGCA^T
HindIII 1396 AAGCTT A^AGCTT
HpaI 1465 GTTAAC GTT^AAC
KasI 63 GGCGCC G^GCGCC
MfeI 1799 CAATTG C^AATTG
MluI 4353 ACGCGT A^CGCGT
MluNI 2900 TGGCCA TGG^CCA
MscI 2900 TGGCCA TGG^CCA
MunI 1799 CAATTG C^AATTG
NaeI 36 GCCGGC GCC^GGC
NarI 63 GGCGCC GG^CGCC
NgoMIV 36 GCCGGC G^CCGGC
NheI 1927 GCTAGC G^CTAGC
NsiI 2101 ATGCAT ATGCA^T
NspV 152 TTCGAA TT^CGAA
PinAI 2275 ACCGGT A^CCGGT
PshAI 642 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1550 AACGTT AA^CGTT
RsrII 57 CGGTCCG CG^G[AT]CCG
SacI 796 GAGCTC GAGCT^C
SalI 3886 GTCGAC G^TCGAC
SapI 2577 GCTCTTC GCTCTTC(1/4)
SexAI 1064 ACCAGGT A^CC[AT]GGT
SfoI 63 GGCGCC GGC^GCC
SfuI 152 TTCGAA TT^CGAA
SspBI 4358 TGTACA T^GTACA
SspI 103 AATATT AAT^ATT
XcmI 1877 CCAGCAAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3949 GAACGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 262 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2833 GACAACAGGTC
Alw44I 1069 GTGCAC G^TGCAC
2620 GTGCAC
AlwNI 331 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
3642 CAGAAGCTG
ApaLI 1069 GTGCAC G^TGCAC
2620 GTGCAC
AspEI 262 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2833 GACAACAGGTC
AvrII 3145 CCTAGG C^CTAGG
3895 CCTAGG
BanI 63 GGCGCC G^G[CT][GA]CC
1624 GGCACC
BbrPI 253 CACGTG CAC^GTG
3204 CACGTG
BclI 398 TGATCA T^GATCA
608 TGATCA
BglI 1939 GCCAATCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2476 GCCGCCATGGC
BlnI 3145 CCTAGG C^CTAGG
3895 CCTAGG
BsaAI 253 CACGTG [CT]AC^GT[GA]
3204 CACGTG
BsiEI 2562 CGACCG CG[GA][CT]^CG
4204 CGGTCG
BsmBI 1629 CGTCTC CGTCTC(1/5)
1279 GAGACG
BspHI 1955 TCATGA T^CATGA
2019 TCATGA
DraI 3256 TTTAAA TTT^AAA
4332 TTTAAA
DraIII 130 CACGACGTG CAC[ACGT][ACGT][ACGT]^GTG
1159 CACGCCGTG
DrdI 496 GACTGGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2143 GACGCCTTGGTC
EaeI 2900 TGGCCA [CT]^GGCC[GA]
2993 TGGCCG
Eam1105I 262 GACCTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2833 GACAACAGGTC
FbaI 398 TGATCA T^GATCA
608 TGATCA
HaeII 63 GGCGCC [GA]GCGC^[CT]
1660 AGCGCC
NcoI 1782 CCATGG C^CATGG
2480 CCATGG
PmaCI 253 CACGTG CAC^GTG
3204 CACGTG
PmlI 253 CACGTG CAC^GTG
3204 CACGTG
PstI 3639 CTGCAG CTGCA^G
4341 CTGCAG
RcaI 1955 TCATGA T^CATGA
2019 TCATGA
XbaI 1647 TCTAGA T^CTAGA
3022 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI ApoI
AspHI AvaI AvaII BamHI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaI BsaJI
BsaWI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII