(Circular) (Six-base) MAPSORT of: pFN21AB4877.seq Check: 5585 from: 1 to: 7123 pFN21AB4877 7123 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4933 278 Size: 53 83 186 936 3397 2468 Fragments arranged by size: 3397 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3365 4466 1937 Size: 1428 1101 4594 Fragments arranged by size: 4594 1428 1101 Acc65I G'GTAC_C Cuts at: 4444 4444 Size: 7123 AclI AA'CG_TT Cuts at: 1563 5250 5623 1563 Size: 3687 373 3063 Fragments arranged by size: 3687 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2057 3147 3378 3441 3902 4288 5186 6570 Size: 425 1090 231 63 461 386 898 1384 Cuts at: 6570 6838 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1090 898 461 425 386 268 231 63 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6926 AflIII A'CryG_T Cuts at: 1203 3167 6022 6999 1203 Size: 1964 2855 977 1327 Fragments arranged by size: 2855 1964 1327 977 AgeI A'CCGG_T Cuts at: 5954 5954 Size: 7123 AhdI GACnn_n'nnGTC Cuts at: 5852 5852 Size: 7123 AleI CACnn'nnGTG Cuts at: 1166 3303 1166 Size: 2137 4986 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7123 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3828 3860 3828 Size: 32 7091 AlwNI CAG_nnn'CTG Cuts at: 3470 3593 4866 6438 3470 Size: 123 1273 1572 4155 Fragments arranged by size: 4155 1572 1273 123 ApaI G_GGCC'C Cuts at: 2363 2363 Size: 7123 ApaLI G'TGCA_C Cuts at: 5180 6336 5180 Size: 1156 5967 ApoI r'AATT_y Cuts at: 1493 3074 3314 3641 3673 4099 4432 4658 4810 Size: 1581 240 327 32 426 333 226 152 Cuts at: 4810 6919 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1581 426 333 327 240 226 152 32 AseI AT'TA_AT Cuts at: 160 1032 5677 160 Size: 872 4645 1606 Fragments arranged by size: 4645 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7123 AvaI C'yCGr_G Cuts at: 1870 1943 2168 2212 2225 2356 2438 2606 4448 Size: 73 225 44 13 131 82 168 1842 Cuts at: 4448 1870 Size: 4545 Fragments arranged by size: 4545 1842 225 168 131 82 73 44 13 BamHI G'GATC_C Cuts at: 4453 4820 4453 Size: 367 6756 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2599 4444 5899 618 Size: 325 217 627 812 1845 1455 1842 Fragments arranged by size: 1845 1842 1455 812 627 325 217 BanII G_rGCy'C Cuts at: 1391 2064 2106 2169 2363 2439 3625 4117 4442 Size: 673 42 63 194 76 1186 492 325 Cuts at: 4442 1391 Size: 4072 Fragments arranged by size: 4072 1186 673 492 325 194 76 63 42 BbeI G_GCGC'C Cuts at: 2603 2603 Size: 7123 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2397 3786 928 Size: 971 498 1389 4265 Fragments arranged by size: 4265 1389 971 498 BbvCI CC'TCA_GC Cuts at: 2763 2763 Size: 7123 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5314 5348 1903 Size: 34 3377 34 3678 Fragments arranged by size: 3678 3377 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5015 6225 5015 Size: 1210 5913 BclI T'GATC_A Cuts at: 1548 1548 Size: 7123 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2345 2549 5734 Size: 107 122 71 631 605 673 204 3185 Cuts at: 5734 136 Size: 1525 Fragments arranged by size: 3185 1525 673 631 605 204 122 107 71 BglII A'GATC_T Cuts at: 3226 7118 3226 Size: 3892 3231 BlpI GC'TnA_GC Cuts at: 4540 4540 Size: 7123 Bme1580I G_kGCm'C Cuts at: 1790 2363 2478 4216 5184 6340 1790 Size: 573 115 1738 968 1156 2573 Fragments arranged by size: 2573 1738 1156 968 573 115 BmgBI CAC'GTC Cuts at: 1318 4073 1318 Size: 2755 4368 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3622 4854 5812 Size: 416 53 83 399 24 2205 1232 958 Cuts at: 5812 442 Size: 1753 Fragments arranged by size: 2205 1753 1232 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7123 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2240 2401 2879 2240 Size: 161 478 6484 Fragments arranged by size: 6484 478 161 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 2931 5783 1239 Size: 863 829 2852 2579 Fragments arranged by size: 2852 2579 863 829 Bpu10I CC'TnA_GC Cuts at: 2763 2763 Size: 7123 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2048 2078 5248 6113 6411 2048 Size: 30 3170 865 298 2760 Fragments arranged by size: 3170 2760 865 298 30 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2096 5786 882 Size: 617 597 3690 2219 Fragments arranged by size: 3690 2219 617 597 BsaAI yAC'GTr Cuts at: 493 1204 4914 493 Size: 711 3710 2702 Fragments arranged by size: 3710 2702 711 BsaBI GATnn'nnATC Cuts at: 7117 7117 Size: 7123 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2074 2600 4930 5312 Size: 53 83 186 936 541 526 2330 382 Cuts at: 5312 5947 275 Size: 635 1451 Fragments arranged by size: 2330 1451 936 635 541 526 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5556 5954 6228 6375 6786 5556 Size: 398 274 147 411 5893 Fragments arranged by size: 5893 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6898 Fragments arranged by size: 6898 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2072 2153 2273 2276 2291 2294 2342 2384 Size: 886 81 120 3 15 3 48 42 Cuts at: 2384 2387 2390 2751 3730 4122 4125 1186 Size: 3 3 361 979 392 3 4184 Fragments arranged by size: 4184 979 886 392 361 120 81 48 42 15 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2204 6326 6822 2204 Size: 4122 496 2505 Fragments arranged by size: 4122 2505 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3327 1163 Size: 573 1591 4959 Fragments arranged by size: 4959 1591 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2244 2352 4573 5334 6362 1540 Size: 275 186 243 108 2221 761 1028 2301 Fragments arranged by size: 2301 2221 1028 761 275 243 186 108 BsiHKAI G_wGCw'C Cuts at: 2064 2106 2439 5184 5269 6340 2064 Size: 42 333 2745 85 1071 2847 Fragments arranged by size: 2847 2745 1071 333 85 42 BsmI GAATG_Cn' Cuts at: 4278 4634 4727 4278 Size: 356 93 6674 Fragments arranged by size: 6674 356 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2867 2867 Size: 7123 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2064 2106 2169 2363 2439 2478 3625 Size: 399 274 42 63 194 76 39 1147 Cuts at: 3625 4117 4216 4442 5184 5269 6340 1391 Size: 492 99 226 742 85 1071 2174 Fragments arranged by size: 2174 1147 1071 742 492 399 274 226 194 99 85 76 63 42 39 BspEI T'CCGG_A Cuts at: 6786 6786 Size: 7123 BspHI T'CATG_A Cuts at: 5012 6742 5012 Size: 1730 5393 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3557 4478 844 Size: 2713 921 3489 Fragments arranged by size: 3489 2713 921 BsrBI CCG'CTC Cuts at: 1394 1823 2297 5010 1394 Size: 429 474 2713 3507 Fragments arranged by size: 3507 2713 474 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5618 5792 65 Size: 1183 4370 174 1396 Fragments arranged by size: 4370 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2243 2500 5767 5954 1700 Size: 110 433 257 3267 187 2869 Fragments arranged by size: 3267 2869 433 257 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7123 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7123 BssSI C'ACGA_G Cuts at: 2694 4209 5183 6195 2694 Size: 1515 974 1012 3622 Fragments arranged by size: 3622 1515 1012 974 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7123 BstBI TT'CG_AA Cuts at: 5927 5927 Size: 7123 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7123 BstXI CCAn_nnnn'nTGG Cuts at: 3635 3635 Size: 7123 BstYI r'GATC_y Cuts at: 1972 3226 4453 4820 5205 5222 6674 6760 6772 Size: 1254 1227 367 385 17 1452 86 12 Cuts at: 6772 7118 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1254 1227 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3366 3366 Size: 7123 BtgI C'CryG_G Cuts at: 513 1065 2160 2238 2340 2553 2710 4231 4391 Size: 552 1095 78 102 213 157 1521 160 Cuts at: 4391 513 Size: 3245 Fragments arranged by size: 3245 1521 1095 552 213 160 157 102 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2265 487 Size: 683 56 1039 5345 Fragments arranged by size: 5345 1039 683 56 BtsI GCAGTG_nn' Cuts at: 4639 5433 5453 4639 Size: 794 20 6309 Fragments arranged by size: 6309 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7123 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4072 4107 4249 4284 529 Size: 35 3508 35 142 35 3368 Fragments arranged by size: 3508 3368 142 35 35 35 DraI TTT'AAA Cuts at: 2845 4427 2845 Size: 1582 5541 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3697 6130 809 Size: 507 2381 2433 1802 Fragments arranged by size: 2433 2381 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2241 2349 2707 Size: 54 1425 50 275 18 411 108 358 Cuts at: 2707 3272 4060 4228 4570 5459 7088 8 Size: 565 788 168 342 889 1629 43 Fragments arranged by size: 1629 1425 889 788 565 411 358 342 275 168 108 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2241 2349 4570 1537 Size: 275 429 108 2221 4090 Fragments arranged by size: 4090 2221 429 275 108 EarI CTCTTCn'nnn_ Cuts at: 1353 2250 2262 2268 2442 2555 2727 2911 3354 Size: 897 12 6 174 113 172 184 443 Cuts at: 3354 3417 3981 5053 1353 Size: 63 564 1072 3423 Fragments arranged by size: 3423 1072 897 564 443 184 174 172 113 63 12 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2679 4146 5710 6084 6230 387 Size: 1064 138 1090 1467 1564 374 146 1280 Fragments arranged by size: 1564 1467 1280 1090 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2062 2104 2437 2062 Size: 42 333 6748 Fragments arranged by size: 6748 333 42 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2102 2931 3147 3378 3441 3902 Size: 393 425 45 829 216 231 63 461 Cuts at: 3902 4288 5186 5783 6570 6838 1239 Size: 386 898 597 787 268 1524 Fragments arranged by size: 1524 898 829 787 597 461 425 393 386 268 231 216 63 45 EcoNI CCTnn'n_nnAGG Cuts at: 2228 3875 2228 Size: 1647 5476 EcoO109I rG'GnC_Cy Cuts at: 3416 3416 Size: 7123 EcoRI G'AATT_C Cuts at: 4432 4810 4432 Size: 378 6745 FspI TGC'GCA Cuts at: 5629 5629 Size: 7123 HaeII r_GCGC'y Cuts at: 1678 2212 2603 6270 1678 Size: 534 391 3667 2531 Fragments arranged by size: 3667 2531 534 391 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5764 5796 5838 5870 1373 Size: 32 135 32 4192 32 42 32 2626 Fragments arranged by size: 4192 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4467 4713 669 Size: 988 281 2529 246 3079 Fragments arranged by size: 3079 2529 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7123 HpaI GTT'AAC Cuts at: 4713 4713 Size: 7123 KasI G'GCGC_C Cuts at: 2599 2599 Size: 7123 KpnI G_GTAC'C Cuts at: 4448 4448 Size: 7123 MfeI C'AATT_G Cuts at: 4722 4722 Size: 7123 MluI A'CGCG_T Cuts at: 6999 6999 Size: 7123 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2025 6237 6421 1305 Size: 720 4212 184 2007 Fragments arranged by size: 4212 2007 720 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4062 10 Size: 54 1425 343 2230 3071 Fragments arranged by size: 3071 2230 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2709 2796 3303 5081 5440 5599 518 Size: 648 1543 87 507 1778 359 159 2042 Fragments arranged by size: 2042 1778 1543 648 507 359 159 87 MspA1I CmG'CkG Cuts at: 1606 2204 2240 2342 2555 2568 4539 5216 6364 Size: 598 36 102 213 13 1971 677 1148 Cuts at: 6364 6609 1606 Size: 245 2120 Fragments arranged by size: 2120 1971 1148 677 598 245 213 102 36 13 NaeI GCC'GGC Cuts at: 2245 2502 2245 Size: 257 6866 NarI GG'CG_CC Cuts at: 2600 2600 Size: 7123 NcoI C'CATG_G Cuts at: 513 1065 2160 4391 513 Size: 552 1095 2231 3245 Fragments arranged by size: 3245 2231 1095 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7123 NgoMIV G'CCGG_C Cuts at: 2243 2500 2243 Size: 257 6866 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7123 NotI GC'GGCC_GC Cuts at: 4570 4570 Size: 7123 NspI r_CATG'y Cuts at: 1132 1229 3114 3171 3290 3500 3842 4156 4481 Size: 97 1885 57 119 210 342 314 325 Cuts at: 4481 6026 1132 Size: 1545 2229 Fragments arranged by size: 2229 1885 1545 342 325 314 210 119 97 57 PasI CC'CwG_GG Cuts at: 1348 4118 1348 Size: 2770 4353 PciI A'CATG_T Cuts at: 3167 6022 3167 Size: 2855 4268 PflMI CCAn_nnn'nTGG Cuts at: 1108 3470 1108 Size: 2362 4761 PfoI T'CCnGG_A Cuts at: 2418 2418 Size: 7123 PmeI GTTT'AAAC Cuts at: 4427 4427 Size: 7123 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3989 4021 5192 5224 6705 6737 3989 Size: 32 1171 32 1481 32 4375 Fragments arranged by size: 4375 1481 1171 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2088 1613 Size: 475 6648 PsiI TTA'TAA Cuts at: 2826 4693 2826 Size: 1867 5256 PspOMI G'GGCC_C Cuts at: 2359 2359 Size: 7123 PspXI vC'TCGA_Gb Cuts at: 2225 2225 Size: 7123 PstI C_TGCA'G Cuts at: 830 4475 830 Size: 3645 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7123 SacI G_AGCT'C Cuts at: 2064 2106 2439 2064 Size: 42 333 6748 Fragments arranged by size: 6748 333 42 SacII CC_GC'GG Cuts at: 2241 2343 2556 2241 Size: 102 213 6808 Fragments arranged by size: 6808 213 102 SalI G'TCGA_C Cuts at: 1936 4465 1936 Size: 2529 4594 SapI GCTCTTCn'nnn_ Cuts at: 2555 2911 3981 2555 Size: 356 1070 5697 Fragments arranged by size: 5697 1070 356 SbfI CC_TGCA'GG Cuts at: 4475 4475 Size: 7123 ScaI AGT'ACT Cuts at: 5371 7083 5371 Size: 1712 5411 SfcI C'TryA_G Cuts at: 826 1045 4471 5606 6287 6478 826 Size: 219 3426 1135 681 191 1471 Fragments arranged by size: 3426 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2601 2601 Size: 7123 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7123 SgrAI Cr'CCGG_yG Cuts at: 1700 2500 1700 Size: 800 6323 SmaI CCC'GGG Cuts at: 1872 2170 2608 4450 1872 Size: 298 438 1842 4545 Fragments arranged by size: 4545 1842 438 298 SmlI C'TyrA_G Cuts at: 820 1017 1943 2063 2093 2225 5227 6128 6390 Size: 197 926 120 30 132 3002 901 262 Cuts at: 6390 820 Size: 1553 Fragments arranged by size: 3002 1553 926 901 262 197 132 120 30 SnaBI TAC'GTA Cuts at: 493 4914 493 Size: 4421 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7123 SphI G_CATG'C Cuts at: 1132 4481 1132 Size: 3349 3774 SspI AAT'ATT Cuts at: 5 52 5047 5 Size: 47 4995 2081 Fragments arranged by size: 4995 2081 47 StuI AGG'CCT Cuts at: 1517 2223 1517 Size: 706 6417 StyI C'CwwG_G Cuts at: 513 1065 2160 2544 4391 4411 4562 513 Size: 552 1095 384 1847 20 151 3074 Fragments arranged by size: 3074 1847 1095 552 384 151 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5150 5167 5320 5505 5950 325 Size: 1255 3570 17 153 185 445 1498 Fragments arranged by size: 3570 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2724 5369 7081 Size: 274 80 33 51 1443 747 2645 1712 Cuts at: 7081 96 Size: 138 Fragments arranged by size: 2645 1712 1443 747 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7083 7115 7083 Size: 32 7091 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7123 XbaI T'CTAG_A Cuts at: 4459 4459 Size: 7123 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4070 4070 Size: 7123 XhoI C'TCGA_G Cuts at: 1943 2225 1943 Size: 282 6841 XmaI C'CCGG_G Cuts at: 1870 2168 2606 4448 1870 Size: 298 438 1842 4545 Fragments arranged by size: 4545 1842 438 298 XmnI GAAnn'nnTTC Cuts at: 5252 5252 Size: 7123 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4931 276 Size: 53 83 186 936 3397 2468 Fragments arranged by size: 3397 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BsiWI Bsu36I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PsrI PvuII RsrII SanDI SexAI SfiI SrfI SwaI