Restriction map for
pFN21AB4877
(Linear) (Six-base) MAPSORT of: KIBB4877.seq Check: 6222 from: 1 to: 2418
KIBB4877.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AccI GT'mk_AC
Cuts at: 0 1360 2418
Size: 1360 1058
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 1142 1373 1436 1897 2283 2418
Size: 52 1090 231 63 461 386 135
Fragments arranged by size:
1090 461 386 231 135 63 52
AflIII A'CryG_T
Cuts at: 0 1162 2418
Size: 1162 1256
AleI CACnn'nnGTG
Cuts at: 0 1298 2418
Size: 1298 1120
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1823 1855 2418
Size: 1823 32 563
Fragments arranged by size:
1823 563 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1465 1588 2418
Size: 1465 123 830
Fragments arranged by size:
1465 830 123
ApaI G_GGCC'C
Cuts at: 0 358 2418
Size: 358 2060
ApoI r'AATT_y
Cuts at: 0 1069 1309 1636 1668 2094 2418
Size: 1069 240 327 32 426 324
Fragments arranged by size:
1069 426 327 324 240 32
AvaI C'yCGr_G
Cuts at: 0 163 207 220 351 433 601 2418
Size: 163 44 13 131 82 168 1817
Fragments arranged by size:
1817 168 163 131 82 44 13
BanI G'GyrC_C
Cuts at: 0 594 2418
Size: 594 1824
BanII G_rGCy'C
Cuts at: 0 59 101 164 358 434 1620 2112 2418
Size: 59 42 63 194 76 1186 492 306
Fragments arranged by size:
1186 492 306 194 76 63 59 42
BbeI G_GCGC'C
Cuts at: 0 598 2418
Size: 598 1820
BbsI GAAGACnn'nnnn_
Cuts at: 0 392 1781 2418
Size: 392 1389 637
Fragments arranged by size:
1389 637 392
BbvCI CC'TCA_GC
Cuts at: 0 758 2418
Size: 758 1660
BglI GCCn_nnn'nGGC
Cuts at: 0 340 544 2418
Size: 340 204 1874
Fragments arranged by size:
1874 340 204
BglII A'GATC_T
Cuts at: 0 1221 2418
Size: 1221 1197
Bme1580I G_kGCm'C
Cuts at: 0 358 473 2211 2418
Size: 358 115 1738 207
Fragments arranged by size:
1738 358 207 115
BmgBI CAC'GTC
Cuts at: 0 2068 2418
Size: 2068 350
BmrI ACTGGGnnnn_n'
Cuts at: 0 1617 2418
Size: 1617 801
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 235 396 874 2418
Size: 235 161 478 1544
Fragments arranged by size:
1544 478 235 161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 926 2418
Size: 97 829 1492
Fragments arranged by size:
1492 829 97
Bpu10I CC'TnA_GC
Cuts at: 0 758 2418
Size: 758 1660
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 73 2418
Size: 43 30 2345
Fragments arranged by size:
2345 43 30
BsaI GGTCTCn'nnnn_
Cuts at: 0 91 2418
Size: 91 2327
BsaHI Gr'CG_yC
Cuts at: 0 69 595 2418
Size: 69 526 1823
Fragments arranged by size:
1823 526 69
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 148 268 271 286 289 337 379
Size: 67 81 120 3 15 3 48 42
Cuts at: 379 382 385 746 1725 2117 2120 2418
Size: 3 3 361 979 392 3 298
Fragments arranged by size:
979 392 361 298 120 81 67 48
42 15 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 199 2418
Size: 199 2219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1322 2418
Size: 1322 1096
BsiEI CG_ry'CG
Cuts at: 0 239 347 2418
Size: 239 108 2071
Fragments arranged by size:
2071 239 108
BsiHKAI G_wGCw'C
Cuts at: 0 59 101 434 2418
Size: 59 42 333 1984
Fragments arranged by size:
1984 333 59 42
BsmI GAATG_Cn'
Cuts at: 0 2273 2418
Size: 2273 145
BsmBI CGTCTCn'nnnn_
Cuts at: 0 862 2418
Size: 862 1556
Bsp1286I G_dGCh'C
Cuts at: 0 59 101 164 358 434 473 1620 2112
Size: 59 42 63 194 76 39 1147 492
Cuts at: 2112 2211 2418
Size: 99 207
Fragments arranged by size:
1147 492 207 194 99 76 63 59
42 39
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1552 2418
Size: 1552 866
BsrBI CCG'CTC
Cuts at: 0 292 2418
Size: 292 2126
BsrFI r'CCGG_y
Cuts at: 0 238 495 2418
Size: 238 257 1923
Fragments arranged by size:
1923 257 238
BssSI C'ACGA_G
Cuts at: 0 689 2204 2418
Size: 689 1515 214
Fragments arranged by size:
1515 689 214
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1630 2418
Size: 1630 788
BstYI r'GATC_y
Cuts at: 0 1221 2418
Size: 1221 1197
BstZ17I GTA'TAC
Cuts at: 0 1361 2418
Size: 1361 1057
BtgI C'CryG_G
Cuts at: 0 155 233 335 548 705 2226 2386 2418
Size: 155 78 102 213 157 1521 160 32
Fragments arranged by size:
1521 213 160 157 155 102 78 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 260 2418
Size: 260 2158
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2067 2102 2244 2279 2418
Size: 2067 35 142 35 139
Fragments arranged by size:
2067 142 139 35 35
DraI TTT'AAA
Cuts at: 0 840 2418
Size: 840 1578
DrdI GACnn_nn'nnGTC
Cuts at: 0 1692 2418
Size: 1692 726
EaeI y'GGCC_r
Cuts at: 0 236 344 702 1267 2055 2223 2418
Size: 236 108 358 565 788 168 195
Fragments arranged by size:
788 565 358 236 195 168 108
EagI C'GGCC_G
Cuts at: 0 236 344 2418
Size: 236 108 2074
Fragments arranged by size:
2074 236 108
EarI CTCTTCn'nnn_
Cuts at: 0 245 257 263 437 550 722 906 1349
Size: 245 12 6 174 113 172 184 443
Cuts at: 1349 1412 1976 2418
Size: 63 564 442
Fragments arranged by size:
564 443 442 245 184 174 172 113
63 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 674 2141 2418
Size: 674 1467 277
Fragments arranged by size:
1467 674 277
EcoICRI GAG'CTC
Cuts at: 0 57 99 432 2418
Size: 57 42 333 1986
Fragments arranged by size:
1986 333 57 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 97 926 1142 1373 1436 1897 2283
Size: 52 45 829 216 231 63 461 386
Cuts at: 2283 2418
Size: 135
Fragments arranged by size:
829 461 386 231 216 135 63 52
45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 223 1870 2418
Size: 223 1647 548
Fragments arranged by size:
1647 548 223
EcoO109I rG'GnC_Cy
Cuts at: 0 1411 2418
Size: 1411 1007
HaeII r_GCGC'y
Cuts at: 0 207 598 2418
Size: 207 391 1820
Fragments arranged by size:
1820 391 207
KasI G'GCGC_C
Cuts at: 0 594 2418
Size: 594 1824
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 20 2418
Size: 20 2398
MscI TGG'CCA
Cuts at: 0 2057 2418
Size: 2057 361
MslI CAynn'nnrTG
Cuts at: 0 704 791 1298 2418
Size: 704 87 507 1120
Fragments arranged by size:
1120 704 507 87
MspA1I CmG'CkG
Cuts at: 0 199 235 337 550 563 2418
Size: 199 36 102 213 13 1855
Fragments arranged by size:
1855 213 199 102 36 13
NaeI GCC'GGC
Cuts at: 0 240 497 2418
Size: 240 257 1921
Fragments arranged by size:
1921 257 240
NarI GG'CG_CC
Cuts at: 0 595 2418
Size: 595 1823
NcoI C'CATG_G
Cuts at: 0 155 2386 2418
Size: 155 2231 32
Fragments arranged by size:
2231 155 32
NgoMIV G'CCGG_C
Cuts at: 0 238 495 2418
Size: 238 257 1923
Fragments arranged by size:
1923 257 238
NspI r_CATG'y
Cuts at: 0 1109 1166 1285 1495 1837 2151 2418
Size: 1109 57 119 210 342 314 267
Fragments arranged by size:
1109 342 314 267 210 119 57
PasI CC'CwG_GG
Cuts at: 0 2113 2418
Size: 2113 305
PciI A'CATG_T
Cuts at: 0 1162 2418
Size: 1162 1256
PflMI CCAn_nnn'nTGG
Cuts at: 0 1465 2418
Size: 1465 953
PfoI T'CCnGG_A
Cuts at: 0 413 2418
Size: 413 2005
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1984 2016 2418
Size: 1984 32 402
Fragments arranged by size:
1984 402 32
PshAI GACnn'nnGTC
Cuts at: 0 83 2418
Size: 83 2335
PsiI TTA'TAA
Cuts at: 0 821 2418
Size: 821 1597
PspOMI G'GGCC_C
Cuts at: 0 354 2418
Size: 354 2064
PspXI vC'TCGA_Gb
Cuts at: 0 220 2418
Size: 220 2198
SacI G_AGCT'C
Cuts at: 0 59 101 434 2418
Size: 59 42 333 1984
Fragments arranged by size:
1984 333 59 42
SacII CC_GC'GG
Cuts at: 0 236 338 551 2418
Size: 236 102 213 1867
Fragments arranged by size:
1867 236 213 102
SapI GCTCTTCn'nnn_
Cuts at: 0 550 906 1976 2418
Size: 550 356 1070 442
Fragments arranged by size:
1070 550 442 356
SfoI GGC'GCC
Cuts at: 0 596 2418
Size: 596 1822
SgrAI Cr'CCGG_yG
Cuts at: 0 495 2418
Size: 495 1923
SmaI CCC'GGG
Cuts at: 0 165 603 2418
Size: 165 438 1815
Fragments arranged by size:
1815 438 165
SmlI C'TyrA_G
Cuts at: 0 58 88 220 2418
Size: 58 30 132 2198
Fragments arranged by size:
2198 132 58 30
StuI AGG'CCT
Cuts at: 0 218 2418
Size: 218 2200
StyI C'CwwG_G
Cuts at: 0 155 539 2386 2406 2418
Size: 155 384 1847 20 12
Fragments arranged by size:
1847 384 155 20 12
TatI w'GTAC_w
Cuts at: 0 719 2418
Size: 719 1699
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2065 2418
Size: 2065 353
XhoI C'TCGA_G
Cuts at: 0 220 2418
Size: 220 2198
XmaI C'CCGG_G
Cuts at: 0 163 601 2418
Size: 163 438 1817
Fragments arranged by size:
1817 438 163
Enzymes that do cut:
AccI AcuI AflIII AleI AloI AlwNI ApaI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BglI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrFI BssSI BstXI BstYI BstZ17I
BtgI BtgZI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PflMI PfoI PpiI PshAI PsiI PspOMI
PspXI SacI SacII SapI SfoI SgrAI SmaI SmlI
StuI StyI TatI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BciVI BclI BfrBI BlpI BmtI BsaAI BsaBI
BsaWI BsaXI BsiWI BspEI BspHI BsrDI BsrGI BssHII
BstAPI BstBI BstEII Bsu36I BtsI ClaI DraIII EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PmeI PmlI PpuMI PsrI PstI PvuI
PvuII RsrII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SgfI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TstI Tth111I XbaI XmnI ZraI