Restriction map for
pFN21AB4875
(Linear) (Six-base) MAPSORT of: KIBB4875.seq Check: 3538 from: 1 to: 3126
KIBB4875.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
Acc65I G'GTAC_C
Cuts at: 0 948 3126
Size: 948 2178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 3126
Size: 425 2701
AfeI AGC'GCT
Cuts at: 0 1532 2137 3126
Size: 1532 605 989
Fragments arranged by size:
1532 989 605
AflIII A'CryG_T
Cuts at: 0 1220 1367 3126
Size: 1220 147 1759
Fragments arranged by size:
1759 1220 147
AleI CACnn'nnGTG
Cuts at: 0 1743 2128 3126
Size: 1743 385 998
Fragments arranged by size:
1743 998 385
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1834 3126
Size: 1834 1292
AlwNI CAG_nnn'CTG
Cuts at: 0 313 1605 1938 2205 2241 2368 2421 2461
Size: 313 1292 333 267 36 127 53 40
Cuts at: 2461 2911 3126
Size: 450 215
Fragments arranged by size:
1292 450 333 313 267 215 127 53
40 36
ApaI G_GGCC'C
Cuts at: 0 145 718 857 1143 1711 2361 3126
Size: 145 573 139 286 568 650 765
Fragments arranged by size:
765 650 573 568 286 145 139
AscI GG'CGCG_CC
Cuts at: 0 2020 2176 3126
Size: 2020 156 950
Fragments arranged by size:
2020 950 156
AvaI C'yCGr_G
Cuts at: 0 505 1703 1765 2170 2221 2840 3126
Size: 505 1198 62 405 51 619 286
Fragments arranged by size:
1198 619 505 405 286 62 51
BanI G'GyrC_C
Cuts at: 0 483 766 823 897 948 1688 1714 1761
Size: 483 283 57 74 51 740 26 47
Cuts at: 1761 1818 1863 2250 2682 3126
Size: 57 45 387 432 444
Fragments arranged by size:
740 483 444 432 387 283 74 57
57 51 47 45 26
BanII G_rGCy'C
Cuts at: 0 31 145 387 506 718 857 913 934
Size: 31 114 242 119 212 139 56 21
Cuts at: 934 1036 1143 1711 1738 1791 2361 3106 3126
Size: 102 107 568 27 53 570 745 20
Fragments arranged by size:
745 570 568 242 212 139 119 114
107 102 56 53 31 27 21 20
BbeI G_GCGC'C
Cuts at: 0 901 1692 1718 1765 1867 2254 2686 3126
Size: 901 791 26 47 102 387 432 440
Fragments arranged by size:
901 791 440 432 387 102 47 26
BbvCI CC'TCA_GC
Cuts at: 0 2692 3126
Size: 2692 434
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1956 1990 2212 2246 3126
Size: 1956 34 222 34 880
Fragments arranged by size:
1956 880 222 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 96 852 1025 1283 1786 1808 1832 2093
Size: 96 756 173 258 503 22 24 261
Cuts at: 2093 2155 2349 3121 3126
Size: 62 194 772 5
Fragments arranged by size:
772 756 503 261 258 194 173 96
62 24 22 5
BlpI GC'TnA_GC
Cuts at: 0 907 933 985 2900 3126
Size: 907 26 52 1915 226
Fragments arranged by size:
1915 907 226 52 26
Bme1580I G_kGCm'C
Cuts at: 0 145 210 216 486 557 718 769 857
Size: 145 65 6 270 71 161 51 88
Cuts at: 857 1143 1389 1711 1823 2361 3126
Size: 286 246 322 112 538 765
Fragments arranged by size:
765 538 322 286 270 246 161 145
112 88 71 65 51 6
BmgBI CAC'GTC
Cuts at: 0 360 1908 3126
Size: 360 1548 1218
Fragments arranged by size:
1548 1218 360
BmrI ACTGGGnnnn_n'
Cuts at: 0 2539 2600 2648 3126
Size: 2539 61 48 478
Fragments arranged by size:
2539 478 61 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 441 2823 2949 3126
Size: 441 2382 126 177
Fragments arranged by size:
2382 441 177 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 646 907 2438 2794 2848 3025 3126
Size: 610 36 261 1531 356 54 177 101
Fragments arranged by size:
1531 610 356 261 177 101 54 36
Bpu10I CC'TnA_GC
Cuts at: 0 1790 2692 2953 3126
Size: 1790 902 261 173
Fragments arranged by size:
1790 902 261 173
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 3126
Size: 352 2774
BsaI GGTCTCn'nnnn_
Cuts at: 0 398 2044 2864 2937 3126
Size: 398 1646 820 73 189
Fragments arranged by size:
1646 820 398 189 73
BsaAI yAC'GTr
Cuts at: 0 894 1221 1368 2130 2713 3126
Size: 894 327 147 762 583 413
Fragments arranged by size:
894 762 583 413 327 147
BsaHI Gr'CG_yC
Cuts at: 0 898 1689 1715 1762 1864 2108 2251 2683
Size: 898 791 26 47 102 244 143 432
Cuts at: 2683 3126
Size: 443
Fragments arranged by size:
898 791 443 432 244 143 102 47
26
BsaWI w'CCGG_w
Cuts at: 0 809 1268 3126
Size: 809 459 1858
Fragments arranged by size:
1858 809 459
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1033 1063 2767 2797 2821 2851 3065 3095
Size: 1033 30 1704 30 24 30 214 30
Cuts at: 3095 3126
Size: 31
Fragments arranged by size:
1704 1033 214 31 30 30 30 30
24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 607 757 1054 1057 1255 2491 2563 3022
Size: 607 150 297 3 198 1236 72 459
Cuts at: 3022 3025 3070 3126
Size: 3 45 56
Fragments arranged by size:
1236 607 459 297 198 150 72 56
45 3 3
BseYI C'CCAG_C
Cuts at: 0 285 711 913 1098 1142 1830 2209 2234
Size: 285 426 202 185 44 688 379 25
Cuts at: 2234 3099 3126
Size: 865 27
Fragments arranged by size:
865 688 426 379 285 202 185 44
27 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 757 1383 2392 2407 2479 3086 3126
Size: 545 212 626 1009 15 72 607 40
Fragments arranged by size:
1009 626 607 545 212 72 40 15
BsiEI CG_ry'CG
Cuts at: 0 578 1553 1844 1878 1956 2295 3126
Size: 578 975 291 34 78 339 831
Fragments arranged by size:
975 831 578 339 291 78 34
BsiHKAI G_wGCw'C
Cuts at: 0 506 632 893 3126
Size: 506 126 261 2233
Fragments arranged by size:
2233 506 261 126
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2146 3126
Size: 2146 980
Bsp1286I G_dGCh'C
Cuts at: 0 31 145 210 216 387 486 506 557
Size: 31 114 65 6 171 99 20 51
Cuts at: 557 632 718 769 857 893 913 934 1036
Size: 75 86 51 88 36 20 21 102
Cuts at: 1036 1143 1389 1711 1738 1791 1823 2361 3106
Size: 107 246 322 27 53 32 538 745
Cuts at: 3106 3126
Size: 20
Fragments arranged by size:
745 538 322 246 171 114 107 102
99 88 86 75 65 53 51 51
36 32 31 27 21 20 20 20
6
BspEI T'CCGG_A
Cuts at: 0 809 1268 3126
Size: 809 459 1858
Fragments arranged by size:
1858 809 459
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 249 479 1613 2981 3126
Size: 249 230 1134 1368 145
Fragments arranged by size:
1368 1134 249 230 145
BsrBI CCG'CTC
Cuts at: 0 1352 1799 2291 3126
Size: 1352 447 492 835
Fragments arranged by size:
1352 835 492 447
BsrFI r'CCGG_y
Cuts at: 0 13 1071 1552 2294 2348 3038 3126
Size: 13 1058 481 742 54 690 88
Fragments arranged by size:
1058 742 690 481 88 54 13
BsrGI T'GTAC_A
Cuts at: 0 1049 2321 3126
Size: 1049 1272 805
Fragments arranged by size:
1272 1049 805
BssHII G'CGCG_C
Cuts at: 0 1561 1755 2020 2176 3126
Size: 1561 194 265 156 950
Fragments arranged by size:
1561 950 265 194 156
BssSI C'ACGA_G
Cuts at: 0 1204 3126
Size: 1204 1922
BstAPI GCAn_nnn'nTGC
Cuts at: 0 369 1605 2421 3126
Size: 369 1236 816 705
Fragments arranged by size:
1236 816 705 369
BstEII G'GTnAC_C
Cuts at: 0 1629 2391 3126
Size: 1629 762 735
Fragments arranged by size:
1629 762 735
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1318 3099 3126
Size: 1318 1781 27
Fragments arranged by size:
1781 1318 27
BstYI r'GATC_y
Cuts at: 0 2364 2466 3126
Size: 2364 102 660
Fragments arranged by size:
2364 660 102
Bsu36I CC'TnA_GG
Cuts at: 0 2876 3126
Size: 2876 250
BtgI C'CryG_G
Cuts at: 0 819 859 952 1577 1950 1956 2003 2040
Size: 819 40 93 625 373 6 47 37
Cuts at: 2040 3126
Size: 1086
Fragments arranged by size:
1086 819 625 373 93 47 40 37
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 179 1118 3126
Size: 179 939 2008
Fragments arranged by size:
2008 939 179
BtsI GCAGTG_nn'
Cuts at: 0 280 562 731 2769 3126
Size: 280 282 169 2038 357
Fragments arranged by size:
2038 357 282 280 169
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2699 2734 3126
Size: 2699 35 392
Fragments arranged by size:
2699 392 35
DraIII CAC_nnn'GTG
Cuts at: 0 820 3126
Size: 820 2306
DrdI GACnn_nn'nnGTC
Cuts at: 0 1638 3126
Size: 1638 1488
EaeI y'GGCC_r
Cuts at: 0 575 1424 1523 1550 1554 1580 1616 1841
Size: 575 849 99 27 4 26 36 225
Cuts at: 1841 1875 1953 1995 2196 2292 2471 2579 3126
Size: 34 78 42 201 96 179 108 547
Fragments arranged by size:
849 575 547 225 201 179 108 99
96 78 42 36 34 27 26 4
EagI C'GGCC_G
Cuts at: 0 575 1550 1841 1875 1953 2292 3126
Size: 575 975 291 34 78 339 834
Fragments arranged by size:
975 834 575 339 291 78 34
EarI CTCTTCn'nnn_
Cuts at: 0 221 268 3126
Size: 221 47 2858
Fragments arranged by size:
2858 221 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1493 3004 3067 3126
Size: 1493 1511 63 59
Fragments arranged by size:
1511 1493 63 59
EcoICRI GAG'CTC
Cuts at: 0 504 3126
Size: 504 2622
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 610 646 907 2438 2794 2848 3025
Size: 425 185 36 261 1531 356 54 177
Cuts at: 3025 3126
Size: 101
Fragments arranged by size:
1531 425 356 261 185 177 101 54
36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2632 2977 3007 3126
Size: 2632 345 30 119
Fragments arranged by size:
2632 345 119 30
EcoO109I rG'GnC_Cy
Cuts at: 0 98 141 182 431 853 854 1483 1707
Size: 98 43 41 249 422 1 629 224
Cuts at: 1707 1988 2357 2358 2458 2541 2696 2768 2970
Size: 281 369 1 100 83 155 72 202
Cuts at: 2970 3126
Size: 156
Fragments arranged by size:
629 422 369 281 249 224 202 156
155 100 98 83 72 43 41 1
1
FseI GG_CCGG'CC
Cuts at: 0 1556 2298 3126
Size: 1556 742 828
Fragments arranged by size:
1556 828 742
FspI TGC'GCA
Cuts at: 0 154 3126
Size: 154 2972
HaeII r_GCGC'y
Cuts at: 0 901 1278 1534 1692 1718 1765 1867 2068
Size: 901 377 256 158 26 47 102 201
Cuts at: 2068 2139 2254 2686 3126
Size: 71 115 432 440
Fragments arranged by size:
901 440 432 377 256 201 158 115
102 71 47 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2821 2840 2853 2872 3126
Size: 2821 19 13 19 254
Fragments arranged by size:
2821 254 19 19 13
KasI G'GCGC_C
Cuts at: 0 897 1688 1714 1761 1863 2250 2682 3126
Size: 897 791 26 47 102 387 432 444
Fragments arranged by size:
897 791 444 432 387 102 47 26
KpnI G_GTAC'C
Cuts at: 0 952 3126
Size: 952 2174
MfeI C'AATT_G
Cuts at: 0 1890 3126
Size: 1890 1236
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1017 2513 2732 3126
Size: 1017 1496 219 394
Fragments arranged by size:
1496 1017 394 219
MscI TGG'CCA
Cuts at: 0 1525 1582 1618 2581 3126
Size: 1525 57 36 963 545
Fragments arranged by size:
1525 963 545 57 36
MslI CAynn'nnrTG
Cuts at: 0 1743 1816 2128 3126
Size: 1743 73 312 998
Fragments arranged by size:
1743 998 312 73
MspA1I CmG'CkG
Cuts at: 0 105 158 276 758 906 917 1732 1938
Size: 105 53 118 482 148 11 815 206
Cuts at: 1938 1958 2031 2230 2418 3099 3126
Size: 20 73 199 188 681 27
Fragments arranged by size:
815 681 482 206 199 188 148 118
105 73 53 27 20 11
NaeI GCC'GGC
Cuts at: 0 15 1554 2296 2350 3126
Size: 15 1539 742 54 776
Fragments arranged by size:
1539 776 742 54 15
NarI GG'CG_CC
Cuts at: 0 898 1689 1715 1762 1864 2251 2683 3126
Size: 898 791 26 47 102 387 432 443
Fragments arranged by size:
898 791 443 432 387 102 47 26
NcoI C'CATG_G
Cuts at: 0 1577 3126
Size: 1577 1549
NgoMIV G'CCGG_C
Cuts at: 0 13 1552 2294 2348 3126
Size: 13 1539 742 54 778
Fragments arranged by size:
1539 778 742 54 13
NruI TCG'CGA
Cuts at: 0 1904 3126
Size: 1904 1222
PasI CC'CwG_GG
Cuts at: 0 1677 3126
Size: 1677 1449
PflMI CCAn_nnn'nTGG
Cuts at: 0 1578 2004 2461 2866 2911 3126
Size: 1578 426 457 405 45 215
Fragments arranged by size:
1578 457 426 405 215 45
PfoI T'CCnGG_A
Cuts at: 0 587 1310 3126
Size: 587 723 1816
Fragments arranged by size:
1816 723 587
PmlI CAC'GTG
Cuts at: 0 894 1221 1368 2130 3126
Size: 894 327 147 762 996
Fragments arranged by size:
996 894 762 327 147
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 595 627 1198 1230 3126
Size: 595 32 571 32 1896
Fragments arranged by size:
1896 595 571 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 431 1988 2768 2970 3126
Size: 431 1557 780 202 156
Fragments arranged by size:
1557 780 431 202 156
PspOMI G'GGCC_C
Cuts at: 0 141 714 853 1139 1707 2357 3126
Size: 141 573 139 286 568 650 769
Fragments arranged by size:
769 650 573 568 286 141 139
PstI C_TGCA'G
Cuts at: 0 246 1727 2030 2339 2966 2978 3050 3126
Size: 246 1481 303 309 627 12 72 76
Fragments arranged by size:
1481 627 309 303 246 76 72 12
PvuII CAG'CTG
Cuts at: 0 906 917 1938 2031 2418 3099 3126
Size: 906 11 1021 93 387 681 27
Fragments arranged by size:
1021 906 681 387 93 27 11
SacI G_AGCT'C
Cuts at: 0 506 3126
Size: 506 2620
SacII CC_GC'GG
Cuts at: 0 1959 3126
Size: 1959 1167
SanDI GG'GwC_CC
Cuts at: 0 431 1988 2768 3126
Size: 431 1557 780 358
Fragments arranged by size:
1557 780 431 358
SapI GCTCTTCn'nnn_
Cuts at: 0 268 3126
Size: 268 2858
SfcI C'TryA_G
Cuts at: 0 242 618 1416 1723 2026 2335 2962 2974
Size: 242 376 798 307 303 309 627 12
Cuts at: 2974 3046 3126
Size: 72 80
Fragments arranged by size:
798 627 376 309 307 303 242 80
72 12
SfoI GGC'GCC
Cuts at: 0 899 1690 1716 1763 1865 2252 2684 3126
Size: 899 791 26 47 102 387 432 442
Fragments arranged by size:
899 791 442 432 387 102 47 26
SmaI CCC'GGG
Cuts at: 0 1767 2172 3126
Size: 1767 405 954
Fragments arranged by size:
1767 954 405
SmlI C'TyrA_G
Cuts at: 0 331 3126
Size: 331 2795
StuI AGG'CCT
Cuts at: 0 527 3126
Size: 527 2599
StyI C'CwwG_G
Cuts at: 0 874 944 1577 2255 3126
Size: 874 70 633 678 871
Fragments arranged by size:
874 871 678 633 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 520 631 957 1453 1669 3123 3126
Size: 520 111 326 496 216 1454 3
Fragments arranged by size:
1454 520 496 326 216 111 3
TatI w'GTAC_w
Cuts at: 0 1013 1049 2321 3126
Size: 1013 36 1272 805
Fragments arranged by size:
1272 1013 805 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 804 836 2767 2799 2929 2961 3065 3097
Size: 804 32 1931 32 130 32 104 32
Cuts at: 3097 3126
Size: 29
Fragments arranged by size:
1931 804 130 104 32 32 32 32
29
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1419 1641 3117 3126
Size: 1419 222 1476 9
Fragments arranged by size:
1476 1419 222 9
XmaI C'CCGG_G
Cuts at: 0 1765 2170 3126
Size: 1765 405 956
Fragments arranged by size:
1765 956 405
XmnI GAAnn'nnTTC
Cuts at: 0 691 3126
Size: 691 2435
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AleI AlfI AlwNI ApaI
AscI AvaI BanI BanII BbeI BbvCI BcgI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI FspI
HaeII Hin4I KasI KpnI MfeI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NruI PasI PflMI
PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI
SacII SanDI SapI SfcI SfoI SmaI SmlI StuI
StyI TaqII TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AloI
ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbsI
BciVI BclI BfrBI BglII BmtI BsaBI BsiWI BsmI
BspHI BsrDI BstBI BstZ17I ClaI DraI EcoRI EcoRV
FalI FspAI HincII HindIII HpaI MluI NdeI NheI
NotI NsiI NspI PacI PciI PmeI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
SwaI Tth111I XbaI XhoI ZraI