(Circular) (Six-base) MAPSORT of: pFN21AB4848.seq Check: 2806 from: 1 to: 5962 pFN21AB4848 5962 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3772 278 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3305 1937 Size: 1368 4594 Acc65I G'GTAC_C Cuts at: 2220 3283 2220 Size: 1063 4899 AclI AA'CG_TT Cuts at: 1563 4089 4462 1563 Size: 2526 373 3063 Fragments arranged by size: 3063 2526 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4025 5409 5677 1632 Size: 2393 1384 268 1917 Fragments arranged by size: 2393 1917 1384 268 AfeI AGC'GCT Cuts at: 2159 2159 Size: 5962 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 4861 5838 1203 Size: 3658 977 1327 Fragments arranged by size: 3658 1327 977 AgeI A'CCGG_T Cuts at: 2217 4793 2217 Size: 2576 3386 AhdI GACnn_n'nnGTC Cuts at: 4691 4691 Size: 5962 AleI CACnn'nnGTG Cuts at: 1166 2246 1166 Size: 1080 4882 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5962 AlwNI CAG_nnn'CTG Cuts at: 3090 3197 3705 5277 3090 Size: 107 508 1572 3775 Fragments arranged by size: 3775 1572 508 107 ApaLI G'TGCA_C Cuts at: 2189 4019 5175 2189 Size: 1830 1156 2976 Fragments arranged by size: 2976 1830 1156 ApoI r'AATT_y Cuts at: 1493 3271 3497 3649 5758 1493 Size: 1778 226 152 2109 1697 Fragments arranged by size: 2109 1778 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4516 160 Size: 872 3484 1606 Fragments arranged by size: 3484 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 3287 1870 Size: 73 1344 4545 Fragments arranged by size: 4545 1344 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2212 2245 2967 3000 2212 Size: 33 722 33 5174 Fragments arranged by size: 5174 722 33 33 BamHI G'GATC_C Cuts at: 3292 3659 3292 Size: 367 5595 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2220 3283 4738 618 Size: 325 217 627 433 1063 1455 1842 Fragments arranged by size: 1842 1455 1063 627 433 325 217 BanII G_rGCy'C Cuts at: 1391 2531 2858 2966 3222 3257 3281 1391 Size: 1140 327 108 256 35 24 4072 Fragments arranged by size: 4072 1140 327 256 108 35 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2272 928 Size: 971 373 4618 Fragments arranged by size: 4618 971 373 BbvCI CC'TCA_GC Cuts at: 3174 3174 Size: 5962 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4153 4187 1903 Size: 34 2216 34 3678 Fragments arranged by size: 3678 2216 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2968 3854 5064 2968 Size: 886 1210 3866 Fragments arranged by size: 3866 1210 886 BclI T'GATC_A Cuts at: 1548 2826 1548 Size: 1278 4684 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3226 4573 136 Size: 107 122 71 631 605 1554 1347 1525 Fragments arranged by size: 1554 1525 1347 631 605 122 107 71 BglII A'GATC_T Cuts at: 5957 5957 Size: 5962 BlpI GC'TnA_GC Cuts at: 2880 3198 3379 2880 Size: 318 181 5463 Fragments arranged by size: 5463 318 181 Bme1580I G_kGCm'C Cuts at: 1790 2193 4023 5179 1790 Size: 403 1830 1156 2573 Fragments arranged by size: 2573 1830 1156 403 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5962 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3693 4651 442 Size: 416 53 83 399 24 2276 958 1753 Fragments arranged by size: 2276 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3119 4622 1239 Size: 1880 1503 2579 Fragments arranged by size: 2579 1880 1503 Bpu10I CC'TnA_GC Cuts at: 3174 3174 Size: 5962 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2240 4087 4952 5250 2240 Size: 1847 865 298 2952 Fragments arranged by size: 2952 1847 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2204 2432 2591 3065 4625 882 Size: 617 705 228 159 474 1560 2219 Fragments arranged by size: 2219 1560 705 617 474 228 159 BsaAI yAC'GTr Cuts at: 493 1204 3753 493 Size: 711 2549 2702 Fragments arranged by size: 2702 2549 711 BsaBI GATnn'nnATC Cuts at: 5956 5956 Size: 5962 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3769 4151 4786 275 Size: 53 83 186 936 2236 382 635 1451 Fragments arranged by size: 2236 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2217 4395 4793 5067 5214 5625 2217 Size: 2178 398 274 147 411 2554 Fragments arranged by size: 2554 2178 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5737 Fragments arranged by size: 5737 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2495 2811 3122 1186 Size: 1309 316 311 4026 Fragments arranged by size: 4026 1309 316 311 BseYI C'CCAG_C Cuts at: 3083 3146 3160 3229 5165 5661 3083 Size: 63 14 69 1936 496 3384 Fragments arranged by size: 3384 1936 496 69 63 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5389 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2803 3412 4173 5201 1540 Size: 275 186 802 609 761 1028 2301 Fragments arranged by size: 2301 1028 802 761 609 275 186 BsiHKAI G_wGCw'C Cuts at: 2193 2966 2988 4023 4108 5179 2193 Size: 773 22 1035 85 1071 2976 Fragments arranged by size: 2976 1071 1035 773 85 22 BsiWI C'GTAC_G Cuts at: 2120 2120 Size: 5962 BsmI GAATG_Cn' Cuts at: 2029 3473 3566 2029 Size: 1444 93 4425 Fragments arranged by size: 4425 1444 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2193 2531 2858 2966 2988 3222 3257 Size: 399 403 338 327 108 22 234 35 Cuts at: 3257 3281 4023 4108 5179 1391 Size: 24 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 403 399 338 327 234 108 85 35 24 22 BspEI T'CCGG_A Cuts at: 5625 5625 Size: 5962 BspHI T'CATG_A Cuts at: 3851 5581 3851 Size: 1730 4232 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2957 3317 844 Size: 2113 360 3489 Fragments arranged by size: 3489 2113 360 BsrBI CCG'CTC Cuts at: 1394 1823 2175 2594 3849 1394 Size: 429 352 419 1255 3507 Fragments arranged by size: 3507 1255 429 419 352 BsrDI GCAATG_nn' Cuts at: 65 1248 2106 2334 4457 4631 65 Size: 1183 858 228 2123 174 1396 Fragments arranged by size: 2123 1396 1183 858 228 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2217 3126 4606 4793 1700 Size: 110 407 909 1480 187 2869 Fragments arranged by size: 2869 1480 909 407 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5962 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5962 BssSI C'ACGA_G Cuts at: 2837 4022 5034 2837 Size: 1185 1012 3765 Fragments arranged by size: 3765 1185 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2949 794 Size: 2155 3807 BstBI TT'CG_AA Cuts at: 4766 4766 Size: 5962 BstEII G'GTnAC_C Cuts at: 1184 2351 1184 Size: 1167 4795 BstXI CCAn_nnnn'nTGG Cuts at: 2248 3103 3229 2248 Size: 855 126 4981 Fragments arranged by size: 4981 855 126 BstYI r'GATC_y Cuts at: 1972 3292 3659 4044 4061 5513 5599 5611 5957 Size: 1320 367 385 17 1452 86 12 346 Cuts at: 5957 1972 Size: 1977 Fragments arranged by size: 1977 1452 1320 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2194 2286 513 Size: 552 939 190 92 4189 Fragments arranged by size: 4189 939 552 190 92 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5223 Fragments arranged by size: 5223 683 56 BtsI GCAGTG_nn' Cuts at: 3155 3478 4272 4292 3155 Size: 323 794 20 4825 Fragments arranged by size: 4825 794 323 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5962 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5927 DraI TTT'AAA Cuts at: 3266 3266 Size: 5962 DraIII CAC_nnn'GTG Cuts at: 2692 2692 Size: 5962 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4969 809 Size: 507 3653 1802 Fragments arranged by size: 3653 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 3409 4298 Size: 54 1425 50 275 18 303 1276 889 Cuts at: 4298 5927 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1276 889 303 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3409 1537 Size: 275 1597 4090 Fragments arranged by size: 4090 1597 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2165 2240 2582 2813 2846 2889 3110 3892 Size: 812 75 342 231 33 43 221 782 Cuts at: 3892 1353 Size: 3423 Fragments arranged by size: 3423 812 782 342 231 221 75 43 33 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4549 4923 5069 387 Size: 1064 138 2960 374 146 1280 Fragments arranged by size: 2960 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2964 2964 Size: 5962 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3119 4025 4622 5409 5677 1239 Size: 393 1487 906 597 787 268 1524 Fragments arranged by size: 1524 1487 906 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2065 2065 Size: 5962 EcoRI G'AATT_C Cuts at: 3271 3649 3271 Size: 378 5584 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2281 2281 Size: 5962 FspI TGC'GCA Cuts at: 4468 4468 Size: 5962 HaeII r_GCGC'y Cuts at: 1678 2161 2266 5109 1678 Size: 483 105 2843 2531 Fragments arranged by size: 2843 2531 483 105 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4603 4635 4677 4709 1373 Size: 32 135 32 3031 32 42 32 2626 Fragments arranged by size: 3031 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3306 3552 669 Size: 988 281 1368 246 3079 Fragments arranged by size: 3079 1368 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5962 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KpnI G_GTAC'C Cuts at: 2224 3287 2224 Size: 1063 4899 MfeI C'AATT_G Cuts at: 3561 3561 Size: 5962 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2637 5076 5260 1305 Size: 1332 2439 184 2007 Fragments arranged by size: 2439 2007 1332 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4140 Fragments arranged by size: 4140 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2246 2630 2777 2942 3920 4279 4438 Size: 648 1080 384 147 165 978 359 159 Cuts at: 4438 518 Size: 2042 Fragments arranged by size: 2042 1080 978 648 384 359 165 159 147 MspA1I CmG'CkG Cuts at: 1606 2884 3087 3378 4055 5203 5448 1606 Size: 1278 203 291 677 1148 245 2120 Fragments arranged by size: 2120 1278 1148 677 291 245 203 NaeI GCC'GGC Cuts at: 3128 3128 Size: 5962 NcoI C'CATG_G Cuts at: 513 1065 2004 2286 513 Size: 552 939 282 4189 Fragments arranged by size: 4189 939 552 282 NdeI CA'TA_TG Cuts at: 387 387 Size: 5962 NgoMIV G'CCGG_C Cuts at: 3126 3126 Size: 5962 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5962 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NspI r_CATG'y Cuts at: 1132 1229 2563 3320 4865 1132 Size: 97 1334 757 1545 2229 Fragments arranged by size: 2229 1545 1334 757 97 PasI CC'CwG_GG Cuts at: 1348 2061 1348 Size: 713 5249 PciI A'CATG_T Cuts at: 4861 4861 Size: 5962 PflMI CCAn_nnn'nTGG Cuts at: 1108 2041 2692 1108 Size: 933 651 4378 Fragments arranged by size: 4378 933 651 PfoI T'CCnGG_A Cuts at: 2205 2205 Size: 5962 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2732 2764 4031 4063 5544 5576 2732 Size: 32 1267 32 1481 32 3118 Fragments arranged by size: 3118 1481 1267 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2065 2065 Size: 5962 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5962 PsiI TTA'TAA Cuts at: 3532 3532 Size: 5962 PstI C_TGCA'G Cuts at: 830 3314 830 Size: 2484 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 PvuII CAG'CTG Cuts at: 2884 3087 2884 Size: 203 5759 SacI G_AGCT'C Cuts at: 2966 2966 Size: 5962 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SanDI GG'GwC_CC Cuts at: 2065 2065 Size: 5962 SapI GCTCTTCn'nnn_ Cuts at: 2165 2846 2165 Size: 681 5281 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 4210 5922 4210 Size: 1712 4250 SfcI C'TryA_G Cuts at: 826 1045 3310 4445 5126 5317 826 Size: 219 2265 1135 681 191 1471 Fragments arranged by size: 2265 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 3289 1872 Size: 1417 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2255 4066 4967 5229 820 Size: 197 926 312 1811 901 262 1553 Fragments arranged by size: 1811 1553 926 901 312 262 197 SnaBI TAC'GTA Cuts at: 493 3753 493 Size: 3260 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 3320 1132 Size: 2188 3774 SspI AAT'ATT Cuts at: 5 52 3886 5 Size: 47 3834 2081 Fragments arranged by size: 3834 2081 47 StuI AGG'CCT Cuts at: 1517 2699 1517 Size: 1182 4780 StyI C'CwwG_G Cuts at: 513 1065 2004 2286 3018 3096 3222 3401 513 Size: 552 939 282 732 78 126 179 3074 Fragments arranged by size: 3074 939 732 552 282 179 126 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2456 3097 3221 3989 4006 4159 4344 Size: 1255 876 641 124 768 17 153 185 Cuts at: 4344 4789 325 Size: 445 1498 Fragments arranged by size: 1498 1255 876 768 641 445 185 153 124 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4208 5920 96 Size: 274 80 33 51 1443 2231 1712 138 Fragments arranged by size: 2231 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2243 2275 2374 2406 5922 5954 2243 Size: 32 99 32 3516 32 2251 Fragments arranged by size: 3516 2251 99 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5962 XbaI T'CTAG_A Cuts at: 2917 3298 2917 Size: 381 5581 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3256 3256 Size: 5962 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 3287 1870 Size: 1417 4545 XmnI GAAnn'nnTTC Cuts at: 4091 4091 Size: 5962 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3770 276 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BbeI BfrBI BplI BsmBI BstZ17I Bsu36I EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI