Restriction map for
pFN21AB4745
(Linear) (Six-base) MAPSORT of: KIBB4745.seq Check: 2688 from: 1 to: 1119
KIBB4745.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AccI GT'mk_AC
Cuts at: 0 839 1119
Size: 839 280
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 1119
Size: 280 839
AfeI AGC'GCT
Cuts at: 0 421 1119
Size: 421 698
AleI CACnn'nnGTG
Cuts at: 0 697 1119
Size: 697 422
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 163 195 1119
Size: 163 32 924
Fragments arranged by size:
924 163 32
AlwNI CAG_nnn'CTG
Cuts at: 0 96 609 1119
Size: 96 513 510
Fragments arranged by size:
513 510 96
ApaI G_GGCC'C
Cuts at: 0 341 1119
Size: 341 778
ApaLI G'TGCA_C
Cuts at: 0 971 1119
Size: 971 148
AvaI C'yCGr_G
Cuts at: 0 345 1119
Size: 345 774
BanII G_rGCy'C
Cuts at: 0 33 341 551 1119
Size: 33 308 210 568
Fragments arranged by size:
568 308 210 33
Bme1580I G_kGCm'C
Cuts at: 0 341 975 1119
Size: 341 634 144
Fragments arranged by size:
634 341 144
BmgBI CAC'GTC
Cuts at: 0 864 1119
Size: 864 255
BmrI ACTGGGnnnn_n'
Cuts at: 0 344 1119
Size: 344 775
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 655 799 937 1119
Size: 479 176 144 138 182
Fragments arranged by size:
479 182 176 144 138
BsaI GGTCTCn'nnnn_
Cuts at: 0 18 1119
Size: 18 1101
BsaAI yAC'GTr
Cuts at: 0 39 1119
Size: 39 1080
BsaHI Gr'CG_yC
Cuts at: 0 871 1119
Size: 871 248
BsaWI w'CCGG_w
Cuts at: 0 590 1119
Size: 590 529
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 163 193 1119
Size: 163 30 926
Fragments arranged by size:
926 163 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 461 577 652 1119
Size: 461 116 75 467
Fragments arranged by size:
467 461 116 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 622 697 708 1119
Size: 19 603 75 11 411
Fragments arranged by size:
603 411 75 19 11
BsiHKAI G_wGCw'C
Cuts at: 0 33 551 795 812 975 1119
Size: 33 518 244 17 163 144
Fragments arranged by size:
518 244 163 144 33 17
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1086 1119
Size: 1086 33
Bsp1286I G_dGCh'C
Cuts at: 0 33 341 551 795 812 975 1119
Size: 33 308 210 244 17 163 144
Fragments arranged by size:
308 244 210 163 144 33 17
BspEI T'CCGG_A
Cuts at: 0 590 1119
Size: 590 529
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 402 1119
Size: 402 717
BsrBI CCG'CTC
Cuts at: 0 621 1119
Size: 621 498
BsrFI r'CCGG_y
Cuts at: 0 727 892 1040 1119
Size: 727 165 148 79
Fragments arranged by size:
727 165 148 79
BstAPI GCAn_nnn'nTGC
Cuts at: 0 609 1119
Size: 609 510
BstXI CCAn_nnnn'nTGG
Cuts at: 0 711 1024 1119
Size: 711 313 95
Fragments arranged by size:
711 313 95
BstYI r'GATC_y
Cuts at: 0 581 1119
Size: 581 538
BtgI C'CryG_G
Cuts at: 0 167 323 914 992 1119
Size: 167 156 591 78 127
Fragments arranged by size:
591 167 156 127 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 894 1119
Size: 894 225
BtsI GCAGTG_nn'
Cuts at: 0 792 1119
Size: 792 327
DraIII CAC_nnn'GTG
Cuts at: 0 42 147 1119
Size: 42 105 972
Fragments arranged by size:
972 105 42
DrdI GACnn_nn'nnGTC
Cuts at: 0 1078 1119
Size: 1078 41
EaeI y'GGCC_r
Cuts at: 0 320 701 917 1119
Size: 320 381 216 202
Fragments arranged by size:
381 320 216 202
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 738 1119
Size: 738 381
EcoICRI GAG'CTC
Cuts at: 0 31 549 1119
Size: 31 518 570
Fragments arranged by size:
570 518 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 479 655 799 937 1119
Size: 280 199 176 144 138 182
Fragments arranged by size:
280 199 182 176 144 138
EcoNI CCTnn'n_nnAGG
Cuts at: 0 592 1119
Size: 592 527
EcoO109I rG'GnC_Cy
Cuts at: 0 5 93 294 338 1071 1119
Size: 5 88 201 44 733 48
Fragments arranged by size:
733 201 88 48 44 5
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 150 243 1119
Size: 150 93 876
Fragments arranged by size:
876 150 93
HaeII r_GCGC'y
Cuts at: 0 423 1119
Size: 423 696
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 653 685 870 902 1119
Size: 653 32 185 32 217
Fragments arranged by size:
653 217 185 32 32
HincII GTy'rAC
Cuts at: 0 930 1119
Size: 930 189
MscI TGG'CCA
Cuts at: 0 322 703 919 1119
Size: 322 381 216 200
Fragments arranged by size:
381 322 216 200
MslI CAynn'nnrTG
Cuts at: 0 115 143 436 673 697 913 1119
Size: 115 28 293 237 24 216 206
Fragments arranged by size:
293 237 216 206 115 28 24
MspA1I CmG'CkG
Cuts at: 0 87 606 945 1119
Size: 87 519 339 174
Fragments arranged by size:
519 339 174 87
NaeI GCC'GGC
Cuts at: 0 894 1042 1119
Size: 894 148 77
Fragments arranged by size:
894 148 77
NcoI C'CATG_G
Cuts at: 0 992 1119
Size: 992 127
NgoMIV G'CCGG_C
Cuts at: 0 892 1040 1119
Size: 892 148 79
Fragments arranged by size:
892 148 79
PflMI CCAn_nnn'nTGG
Cuts at: 0 147 1119
Size: 147 972
PmlI CAC'GTG
Cuts at: 0 39 1119
Size: 39 1080
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 163 195 1119
Size: 163 32 924
Fragments arranged by size:
924 163 32
PpuMI rG'GwC_Cy
Cuts at: 0 5 1071 1119
Size: 5 1066 48
Fragments arranged by size:
1066 48 5
PspOMI G'GGCC_C
Cuts at: 0 337 1119
Size: 337 782
PstI C_TGCA'G
Cuts at: 0 92 284 1119
Size: 92 192 835
Fragments arranged by size:
835 192 92
PvuII CAG'CTG
Cuts at: 0 87 606 1119
Size: 87 519 513
Fragments arranged by size:
519 513 87
SacI G_AGCT'C
Cuts at: 0 33 551 1119
Size: 33 518 568
Fragments arranged by size:
568 518 33
SanDI GG'GwC_CC
Cuts at: 0 5 1119
Size: 5 1114
SexAI A'CCwGG_T
Cuts at: 0 710 1119
Size: 710 409
SfcI C'TryA_G
Cuts at: 0 88 280 1119
Size: 88 192 839
Fragments arranged by size:
839 192 88
SmaI CCC'GGG
Cuts at: 0 347 1119
Size: 347 772
StuI AGG'CCT
Cuts at: 0 391 958 1119
Size: 391 567 161
Fragments arranged by size:
567 391 161
StyI C'CwwG_G
Cuts at: 0 992 1119
Size: 992 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 40 1119
Size: 40 1079
TatI w'GTAC_w
Cuts at: 0 665 1119
Size: 665 454
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 965 997 1119
Size: 965 32 122
Fragments arranged by size:
965 122 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 912 1119
Size: 912 207
XmaI C'CCGG_G
Cuts at: 0 345 1119
Size: 345 774
Enzymes that do cut:
AccI AcuI AfeI AleI AloI AlwNI ApaI ApaLI
AvaI BanII Bme1580I BmgBI BmrI BpmI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrFI BstAPI BstXI BstYI BtgI
BtgZI BtsI DraIII DrdI EaeI EciI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII MscI MslI
MspA1I NaeI NcoI NgoMIV PflMI PmlI PpiI PpuMI
PspOMI PstI PvuII SacI SanDI SexAI SfcI SmaI
StuI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AlfI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmtI BplI Bpu10I BpuEI BsaBI
BseYI BsiEI BsiWI BsmI BspHI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI
EagI EarI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI MmeI NarI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XhoI XmnI ZraI