Restriction map for
pFN21AB4737
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# NO CUT ENZYMES ( 86 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AspI AviII
BamHI BbeI BcgI BfrI BglII
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrGI BssHII BstBI ClaI
CpoI DraI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoRV EcoT22I
FseI FspI HaeII HindIII HpaI
KasI KpnI Ksp632I MamI MluI
MroI NarI NdeI NheI NotI
NsiI NspV PacI PflFI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI SapI ScaI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI SspBI SwaI Tth111I XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 4063 GAATTC [GA]^AATT[CT]
AcyI 1243 GGCGTC G[GA]^CG[CT]C
Alw44I 2882 GTGCAC G^TGCAC
ApaLI 2882 GTGCAC G^TGCAC
ApoI 4063 GAATTC [GA]^AATT[CT]
Asp700I 2446 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 3154 CCTAGG C^CTAGG
BalI 3779 TGGCCA TGG^CCA
BbrPI 119 CACGTG CAC^GTG
BbvCI 3904 GCTGAGG CCTCAGC(-5/-2)
BclI 3117 TGATCA T^GATCA
BlnI 3154 CCTAGG C^CTAGG
BsaAI 119 CACGTG [CT]AC^GT[GA]
BsaHI 1243 GGCGTC G[GA]^CG[CT]C
BsrBI 1068 GAGCGG CCGCTC(-3/-3)
Bst1107I 3577 GTATAC GTA^TAC
BstAPI 3084 GCAAGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 3740 CCAAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1162 GGATCT [GA]^GATC[CT]
BstZ17I 3577 GTATAC GTA^TAC
EcoRI 4063 GAATTC G^AATTC
FbaI 3117 TGATCA T^GATCA
HgaI 1244 GCGTC GACGC(5/10)
Hin1I 1243 GGCGTC G[GA]^CG[CT]C
KspI 978 CCGCGG CCGC^GG
MfeI 1436 CAATTG C^AATTG
MflI 1162 GGATCT [GA]^GATC[CT]
MluNI 3779 TGGCCA TGG^CCA
MscI 3779 TGGCCA TGG^CCA
MslI 3109 CACCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1436 CAATTG C^AATTG
NruI 2811 TCGCGA TCG^CGA
PaeR7I 2631 CTCGAG C^TCGAG
PflMI 3638 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 119 CACGTG CAC^GTG
PmlI 119 CACGTG CAC^GTG
PshAI 2470 GACCAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3654 GAGCTC GAGCT^C
SacII 978 CCGCGG CCGC^GG
SbfI 382 CCTGCAGG CCTGCA^GG
Sse8387I 382 CCTGCAGG CCTGCA^GG
SspI 2857 AATATT AAT^ATT
TfiI 1478 GATTC G^A[AT]TC
Van91I 3638 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3463 TCTAGA T^CTAGA
XhoI 2631 CTCGAG C^TCGAG
XhoII 1162 GGATCT [GA]^GATC[CT]
XmnI 2446 GAAGGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 2648 GTCTAC GT^[AC][GT]AC
3577 GTATAC
AfaI 2558 GTAC GT^AC
3215 GTAC
AflIII 669 ACGTGT A^C[GA][CT]GT
3672 ACACGT
AlwI 1507 GATCC GGATC(4/5)
1162 GGATC
BciVI 15 GGATAC GTATCC(6/5)
3608 GTATCC
BlpI 3 GCTCAGC GC^T[ACGT]AGC
3368 GCTCAGC
Bpu1102I 3 GCTCAGC GC^T[ACGT]AGC
3368 GCTCAGC
BsgI 308 GTGCAG GTGCAG(16/14)
2504 GTGCAG
BsmI 3008 GCATTC GAATGC(1/-1)
3233 GCATTC
BsrDI 2694 CATTGC GCAATG(2/0)
3825 CATTGC
CelII 3 GCTCAGC GC^T[ACGT]AGC
3368 GCTCAGC
CfoI 2841 GCGC GCG^C
3771 GCGC
EaeI 2484 TGGCCG [CT]^GGCC[GA]
3779 TGGCCA
EcoNI 3544 CCTAACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3855 CCTGAAAAAGG
HhaI 2841 GCGC GCG^C
3771 GCGC
HinP1I 2841 GCGC G^CGC
3771 GCGC
MseI 283 TTAA T^TAA
3064 TTAA
NaeI 1168 GCCGGC GCC^GGC
2698 GCCGGC
NcoI 1725 CCATGG C^CATGG
3111 CCATGG
NgoMIV 1168 GCCGGC G^CCGGC
2698 GCCGGC
NspI 61 ACATGC [GA]CATG^[CT]
3484 ACATGC
PstI 383 CTGCAG CTGCA^G
2876 CTGCAG
RsaI 2558 GTAC GT^AC
3215 GTAC
StuI 864 AGGCCT AGG^CCT
1426 AGGCCT
Tru9I 283 TTAA T^TAA
3064 TTAA
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# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AluI AlwNI AocI
ApaI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BssSI BstEII
BstF5I BstNI BstPI BstUI Bsu36I
Cac8I Cfr10I Cfr13I DdeI DpnII
DraII DraIII DrdI DsaI Eco81I
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PvuII Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfiI
SmlI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI