Restriction map for pFN21AB4712
 (Circular) (Six-base) MAPSORT of: pFN21AB4712.seq  Check: 6991  from: 1  to: 5446



pFN21AB4712  5446 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:25  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3256     278 
   Size:         53      83     186     936    1720    2468

  Fragments arranged by size: 

                2468    1720     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2789    1937 
   Size:        852    4594
 
Acc65I G'GTAC_C

Cuts at:   2767    2767 
   Size:       5446
 
AclI AA'CG_TT

Cuts at:   1563    2157    3573    3946    1563 
   Size:        594    1416     373    3063

  Fragments arranged by size: 

                3063    1416     594     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2396    2430    3509    4893    5161    1632 
   Size:        764      34    1079    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1079     764     268      34
 
AfeI AGC'GCT

Cuts at:   2060    2060 
   Size:       5446
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5249
 
AflIII A'CryG_T

Cuts at:   1203    4345    5322    1203 
   Size:       3142     977    1327

  Fragments arranged by size: 

                3142    1327     977
 
AgeI A'CCGG_T

Cuts at:   4277    4277 
   Size:       5446
 
AhdI GACnn_n'nnGTC

Cuts at:   4175    4175 
   Size:       5446
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5446
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5446
 
AlwNI CAG_nnn'CTG

Cuts at:   3189    4761    3189 
   Size:       1572    3874
 
ApaLI G'TGCA_C

Cuts at:   3503    4659    3503 
   Size:       1156    4290
 
ApoI r'AATT_y

Cuts at:   1493    2755    2981    3133    5242    1493 
   Size:       1262     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1262     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4000     160 
   Size:        872    2968    1606

  Fragments arranged by size: 

                2968    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5446
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2771    1870 
   Size:         73     828    4545

  Fragments arranged by size: 

                4545     828      73
 
BamHI G'GATC_C

Cuts at:   2776    3143    2776 
   Size:        367    5079
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2767    4222     618 
   Size:        325     217     627     980    1455    1842

  Fragments arranged by size: 

                1842    1455     980     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2765    1391 
   Size:       1374    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2216    2539     928 
   Size:        971     317     323    3835

  Fragments arranged by size: 

                3835     971     323     317
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3637    3671    1903 
   Size:         34    1700      34    3678

  Fragments arranged by size: 

                3678    1700      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3338    4548    3338 
   Size:       1210    4236
 
BclI T'GATC_A

Cuts at:   1548    2328    1548 
   Size:        780    4666
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2042    2051    4057 
   Size:        107     122      71     631     605     370       9    2006

Cuts at:   4057     136 
   Size:       1525

  Fragments arranged by size: 

                2006    1525     631     605     370     122     107      71
                   9
 
BglII A'GATC_T

Cuts at:   5441    5441 
   Size:       5446
 
BlpI GC'TnA_GC

Cuts at:   2306    2863    2306 
   Size:        557    4889
 
Bme1580I G_kGCm'C

Cuts at:   1790    2170    3507    4663    1790 
   Size:        380    1337    1156    2573

  Fragments arranged by size: 

                2573    1337    1156     380
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5446
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2344    3177    4135 
   Size:        416      53      83     399      24     927     833     958

Cuts at:   4135     442 
   Size:       1753

  Fragments arranged by size: 

                1753     958     927     833     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5446
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2203    4106    1239 
   Size:        964    1903    2579

  Fragments arranged by size: 

                2579    1903     964
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3571    4436    4734    3571 
   Size:        865     298    4283

  Fragments arranged by size: 

                4283     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2339    4109     882 
   Size:        617     840    1770    2219

  Fragments arranged by size: 

                2219    1770     840     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3237     493 
   Size:        711    2033    2702

  Fragments arranged by size: 

                2702    2033     711
 
BsaBI GATnn'nnATC

Cuts at:   5440    5440 
   Size:       5446
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2613    3253    3635    4270 
   Size:         53      83     186     936    1080     640     382     635

Cuts at:   4270     275 
   Size:       1451

  Fragments arranged by size: 

                1451    1080     936     640     635     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   2420    3879    4277    4551    4698    5109    2420 
   Size:       1459     398     274     147     411    2757

  Fragments arranged by size: 

                2757    1459     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2276    2306    2630    2642    2660 
   Size:         30     165      30     873      30     324      12      18

Cuts at:   2660    2672    1178 
   Size:         12    3952

  Fragments arranged by size: 

                3952     873     324     165      30      30      30      18
                  12      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2669    1186 
   Size:       1483    3963
 
BseYI C'CCAG_C

Cuts at:   4649    5145    4649 
   Size:        496    4950
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2048    1163 
   Size:        573     312    4561

  Fragments arranged by size: 

                4561     573     312
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2896    3657    4685    1540 
   Size:        275     186     895     761    1028    2301

  Fragments arranged by size: 

                2301    1028     895     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2496    3507    3592    4663    2496 
   Size:       1011      85    1071    3279

  Fragments arranged by size: 

                3279    1071    1011      85
 
BsmI GAATG_Cn'

Cuts at:   2957    3050    2957 
   Size:         93    5353
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2170    2496    2765    3507    3592    4663    1391 
   Size:        399     380     326     269     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     380     326     269      85
            
 
BspEI T'CCGG_A

Cuts at:   5109    5109 
   Size:       5446
 
BspHI T'CATG_A

Cuts at:   3335    5065    3335 
   Size:       1730    3716
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2801     844 
   Size:       1957    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2175    3333    1394 
   Size:        429     352    1158    3507

  Fragments arranged by size: 

                3507    1158     429     352
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2233    3941    4115      65 
   Size:       1183     985    1708     174    1396

  Fragments arranged by size: 

                1708    1396    1183     985     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4090    4277    1700 
   Size:        110    2280     187    2869

  Fragments arranged by size: 

                2869    2280     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5446
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5446
 
BssSI C'ACGA_G

Cuts at:   2477    3506    4518    2477 
   Size:       1029    1012    3405

  Fragments arranged by size: 

                3405    1029    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2026     794 
   Size:       1232    4214
 
BstBI TT'CG_AA

Cuts at:   4250    4250 
   Size:       5446
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5446
 
BstYI r'GATC_y

Cuts at:   1972    2559    2776    3143    3528    3545    4997    5083    5095 
   Size:        587     217     367     385      17    1452      86      12

Cuts at:   5095    5441    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     587     385     367     346     217      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2076     513 
   Size:        552     939      72    3883

  Fragments arranged by size: 

                3883     939     552      72
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2602     487 
   Size:        683      56    1376    3331

  Fragments arranged by size: 

                3331    1376     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2962    3756    3776    2962 
   Size:        794      20    4632

  Fragments arranged by size: 

                4632     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5446
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5411
 
DraI TTT'AAA

Cuts at:   2750    2750 
   Size:       5446
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4453     809 
   Size:        507    3137    1802

  Fragments arranged by size: 

                3137    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2043    2073    2079 
   Size:         54    1425      50     275      18     213      30       6

Cuts at:   2079    2415    2592    2893    3782    5411       8 
   Size:        336     177     301     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     336     301     275     213     177
                  54      50      43      30      18       6
 
EagI C'GGCC_G

Cuts at:   1537    1812    2893    1537 
   Size:        275    1081    4090

  Fragments arranged by size: 

                4090    1081     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2182    3376    1353 
   Size:        829    1194    3423

  Fragments arranged by size: 

                3423    1194     829
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2069    4033    4407    4553     387 
   Size:       1064     138     480    1964     374     146    1280

  Fragments arranged by size: 

                1964    1280    1064     480     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2203    2396    2430    3509    4106    4893    5161 
   Size:        393     571     193      34    1079     597     787     268

Cuts at:   5161    1239 
   Size:       1524

  Fragments arranged by size: 

                1524    1079     787     597     571     393     268     193
                  34
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2639    2639 
   Size:       5446
 
EcoRI G'AATT_C

Cuts at:   2755    3133    2755 
   Size:        378    5068
 
FspI TGC'GCA

Cuts at:   3952    3952 
   Size:       5446
 
HaeII r_GCGC'y

Cuts at:   1678    2062    4593    1678 
   Size:        384    2531    2531

  Fragments arranged by size: 

                2531    2531     384
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2099    2131    4087    4119    4161 
   Size:         32     135      32     527      32    1956      32      42

Cuts at:   4161    4193    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1956     527     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2790    3036     669 
   Size:        988     281     852     246    3079

  Fragments arranged by size: 

                3079     988     852     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5446
 
HpaI GTT'AAC

Cuts at:   3036    3036 
   Size:       5446
 
KpnI G_GTAC'C

Cuts at:   2771    2771 
   Size:       5446
 
MfeI C'AATT_G

Cuts at:   3045    3045 
   Size:       5446
 
MluI A'CGCG_T

Cuts at:   5322    5322 
   Size:       5446
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2262    4560    4744    1305 
   Size:        957    2298     184    2007

  Fragments arranged by size: 

                2298    2007     957     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2417    2594      10 
   Size:         54    1425     343     585     177    2862

  Fragments arranged by size: 

                2862    1425     585     343     177      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3404    3763    3922     518 
   Size:        648    2238     359     159    2042

  Fragments arranged by size: 

                2238    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2078    2862    3539    4687    4932    1606 
   Size:        472     784     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     784     677     472     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3955

  Fragments arranged by size: 

                3955     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5446
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5446
 
NotI GC'GGCC_GC

Cuts at:   2893    2893 
   Size:       5446
 
NspI r_CATG'y

Cuts at:   1132    1229    2500    2804    4349    1132 
   Size:         97    1271     304    1545    2229

  Fragments arranged by size: 

                2229    1545    1271     304      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5446
 
PciI A'CATG_T

Cuts at:   4345    4345 
   Size:       5446
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5446
 
PfoI T'CCnGG_A

Cuts at:   2180    2180 
   Size:       5446
 
PmeI GTTT'AAAC

Cuts at:   2750    2750 
   Size:       5446
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3515    3547    5028    5060    3515 
   Size:         32    1481      32    3901

  Fragments arranged by size: 

                3901    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5446
 
PsiI TTA'TAA

Cuts at:   3016    3016 
   Size:       5446
 
PstI C_TGCA'G

Cuts at:    830    2221    2798     830 
   Size:       1391     577    3478

  Fragments arranged by size: 

                3478    1391     577
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5446
 
SacII CC_GC'GG

Cuts at:   2079    2079 
   Size:       5446
 
SalI G'TCGA_C

Cuts at:   1936    2788    1936 
   Size:        852    4594
 
SapI GCTCTTCn'nnn_

Cuts at:   2182    2182 
   Size:       5446
 
SbfI CC_TGCA'GG

Cuts at:   2798    2798 
   Size:       5446
 
ScaI AGT'ACT

Cuts at:   3694    5406    3694 
   Size:       1712    3734
 
SfcI C'TryA_G

Cuts at:    826    1045    2217    2464    2794    3929    4610    4801     826 
   Size:        219    1172     247     330    1135     681     191    1471

  Fragments arranged by size: 

                1471    1172    1135     681     330     247     219     191
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5446
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5446
 
SmaI CCC'GGG

Cuts at:   1872    2773    1872 
   Size:        901    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3550    4451    4713     820 
   Size:        197     926    1607     901     262    1553

  Fragments arranged by size: 

                1607    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3237     493 
   Size:       2744    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5446
 
SphI G_CATG'C

Cuts at:   1132    2804    1132 
   Size:       1672    3774
 
SspI AAT'ATT

Cuts at:      5      52    3370       5 
   Size:         47    3318    2081

  Fragments arranged by size: 

                3318    2081      47
 
StuI AGG'CCT

Cuts at:   1517    2462    2526    1517 
   Size:        945      64    4437

  Fragments arranged by size: 

                4437     945      64
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2885     513 
   Size:        552     939     881    3074

  Fragments arranged by size: 

                3074     939     881     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2522    3473    3490    3643    3828    4273     325 
   Size:       1255     942     951      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255     951     942     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3692    5404      96 
   Size:        274      80      33      51    1443    1715    1712     138

  Fragments arranged by size: 

                1715    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2276    2308    5406    5438    2276 
   Size:         32    3098      32    2284

  Fragments arranged by size: 

                3098    2284      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5446
 
XbaI T'CTAG_A

Cuts at:   2782    2782 
   Size:       5446
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5446
 
XmaI C'CCGG_G

Cuts at:   1870    2771    1870 
   Size:        901    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3575    3575 
   Size:       5446
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3254     276 
   Size:         53      83     186     936    1720    2468

  Fragments arranged by size: 

                2468    1720     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoNI    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PshAI     PsiI     PstI     PvuI    SacII     SalI
     SapI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AloI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI EcoO109I    EcoRV     FalI     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PmlI    PpuMI
   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI    SanDI    SexAI
     SfiI     SfoI     SrfI     SwaI     XcmI
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