(Circular) (Six-base) MAPSORT of: pFN21AB4712.seq Check: 6991 from: 1 to: 5446 pFN21AB4712 5446 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3256 278 Size: 53 83 186 936 1720 2468 Fragments arranged by size: 2468 1720 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2789 1937 Size: 852 4594 Acc65I G'GTAC_C Cuts at: 2767 2767 Size: 5446 AclI AA'CG_TT Cuts at: 1563 2157 3573 3946 1563 Size: 594 1416 373 3063 Fragments arranged by size: 3063 1416 594 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2396 2430 3509 4893 5161 1632 Size: 764 34 1079 1384 268 1917 Fragments arranged by size: 1917 1384 1079 764 268 34 AfeI AGC'GCT Cuts at: 2060 2060 Size: 5446 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5249 AflIII A'CryG_T Cuts at: 1203 4345 5322 1203 Size: 3142 977 1327 Fragments arranged by size: 3142 1327 977 AgeI A'CCGG_T Cuts at: 4277 4277 Size: 5446 AhdI GACnn_n'nnGTC Cuts at: 4175 4175 Size: 5446 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5446 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5446 AlwNI CAG_nnn'CTG Cuts at: 3189 4761 3189 Size: 1572 3874 ApaLI G'TGCA_C Cuts at: 3503 4659 3503 Size: 1156 4290 ApoI r'AATT_y Cuts at: 1493 2755 2981 3133 5242 1493 Size: 1262 226 152 2109 1697 Fragments arranged by size: 2109 1697 1262 226 152 AseI AT'TA_AT Cuts at: 160 1032 4000 160 Size: 872 2968 1606 Fragments arranged by size: 2968 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5446 AvaI C'yCGr_G Cuts at: 1870 1943 2771 1870 Size: 73 828 4545 Fragments arranged by size: 4545 828 73 BamHI G'GATC_C Cuts at: 2776 3143 2776 Size: 367 5079 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2767 4222 618 Size: 325 217 627 980 1455 1842 Fragments arranged by size: 1842 1455 980 627 325 217 BanII G_rGCy'C Cuts at: 1391 2765 1391 Size: 1374 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2216 2539 928 Size: 971 317 323 3835 Fragments arranged by size: 3835 971 323 317 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3637 3671 1903 Size: 34 1700 34 3678 Fragments arranged by size: 3678 1700 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3338 4548 3338 Size: 1210 4236 BclI T'GATC_A Cuts at: 1548 2328 1548 Size: 780 4666 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2042 2051 4057 Size: 107 122 71 631 605 370 9 2006 Cuts at: 4057 136 Size: 1525 Fragments arranged by size: 2006 1525 631 605 370 122 107 71 9 BglII A'GATC_T Cuts at: 5441 5441 Size: 5446 BlpI GC'TnA_GC Cuts at: 2306 2863 2306 Size: 557 4889 Bme1580I G_kGCm'C Cuts at: 1790 2170 3507 4663 1790 Size: 380 1337 1156 2573 Fragments arranged by size: 2573 1337 1156 380 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5446 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2344 3177 4135 Size: 416 53 83 399 24 927 833 958 Cuts at: 4135 442 Size: 1753 Fragments arranged by size: 1753 958 927 833 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5446 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2203 4106 1239 Size: 964 1903 2579 Fragments arranged by size: 2579 1903 964 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3571 4436 4734 3571 Size: 865 298 4283 Fragments arranged by size: 4283 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2339 4109 882 Size: 617 840 1770 2219 Fragments arranged by size: 2219 1770 840 617 BsaAI yAC'GTr Cuts at: 493 1204 3237 493 Size: 711 2033 2702 Fragments arranged by size: 2702 2033 711 BsaBI GATnn'nnATC Cuts at: 5440 5440 Size: 5446 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2613 3253 3635 4270 Size: 53 83 186 936 1080 640 382 635 Cuts at: 4270 275 Size: 1451 Fragments arranged by size: 1451 1080 936 640 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2420 3879 4277 4551 4698 5109 2420 Size: 1459 398 274 147 411 2757 Fragments arranged by size: 2757 1459 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2276 2306 2630 2642 2660 Size: 30 165 30 873 30 324 12 18 Cuts at: 2660 2672 1178 Size: 12 3952 Fragments arranged by size: 3952 873 324 165 30 30 30 18 12 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2669 1186 Size: 1483 3963 BseYI C'CCAG_C Cuts at: 4649 5145 4649 Size: 496 4950 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2048 1163 Size: 573 312 4561 Fragments arranged by size: 4561 573 312 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2896 3657 4685 1540 Size: 275 186 895 761 1028 2301 Fragments arranged by size: 2301 1028 895 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2496 3507 3592 4663 2496 Size: 1011 85 1071 3279 Fragments arranged by size: 3279 1071 1011 85 BsmI GAATG_Cn' Cuts at: 2957 3050 2957 Size: 93 5353 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2170 2496 2765 3507 3592 4663 1391 Size: 399 380 326 269 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 380 326 269 85 BspEI T'CCGG_A Cuts at: 5109 5109 Size: 5446 BspHI T'CATG_A Cuts at: 3335 5065 3335 Size: 1730 3716 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2801 844 Size: 1957 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2175 3333 1394 Size: 429 352 1158 3507 Fragments arranged by size: 3507 1158 429 352 BsrDI GCAATG_nn' Cuts at: 65 1248 2233 3941 4115 65 Size: 1183 985 1708 174 1396 Fragments arranged by size: 1708 1396 1183 985 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4090 4277 1700 Size: 110 2280 187 2869 Fragments arranged by size: 2869 2280 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5446 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5446 BssSI C'ACGA_G Cuts at: 2477 3506 4518 2477 Size: 1029 1012 3405 Fragments arranged by size: 3405 1029 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2026 794 Size: 1232 4214 BstBI TT'CG_AA Cuts at: 4250 4250 Size: 5446 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5446 BstYI r'GATC_y Cuts at: 1972 2559 2776 3143 3528 3545 4997 5083 5095 Size: 587 217 367 385 17 1452 86 12 Cuts at: 5095 5441 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 587 385 367 346 217 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2076 513 Size: 552 939 72 3883 Fragments arranged by size: 3883 939 552 72 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2602 487 Size: 683 56 1376 3331 Fragments arranged by size: 3331 1376 683 56 BtsI GCAGTG_nn' Cuts at: 2962 3756 3776 2962 Size: 794 20 4632 Fragments arranged by size: 4632 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5446 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5411 DraI TTT'AAA Cuts at: 2750 2750 Size: 5446 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4453 809 Size: 507 3137 1802 Fragments arranged by size: 3137 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2073 2079 Size: 54 1425 50 275 18 213 30 6 Cuts at: 2079 2415 2592 2893 3782 5411 8 Size: 336 177 301 889 1629 43 Fragments arranged by size: 1629 1425 889 336 301 275 213 177 54 50 43 30 18 6 EagI C'GGCC_G Cuts at: 1537 1812 2893 1537 Size: 275 1081 4090 Fragments arranged by size: 4090 1081 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2182 3376 1353 Size: 829 1194 3423 Fragments arranged by size: 3423 1194 829 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2069 4033 4407 4553 387 Size: 1064 138 480 1964 374 146 1280 Fragments arranged by size: 1964 1280 1064 480 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2203 2396 2430 3509 4106 4893 5161 Size: 393 571 193 34 1079 597 787 268 Cuts at: 5161 1239 Size: 1524 Fragments arranged by size: 1524 1079 787 597 571 393 268 193 34 EcoNI CCTnn'n_nnAGG Cuts at: 2639 2639 Size: 5446 EcoRI G'AATT_C Cuts at: 2755 3133 2755 Size: 378 5068 FspI TGC'GCA Cuts at: 3952 3952 Size: 5446 HaeII r_GCGC'y Cuts at: 1678 2062 4593 1678 Size: 384 2531 2531 Fragments arranged by size: 2531 2531 384 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2099 2131 4087 4119 4161 Size: 32 135 32 527 32 1956 32 42 Cuts at: 4161 4193 1373 Size: 32 2626 Fragments arranged by size: 2626 1956 527 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2790 3036 669 Size: 988 281 852 246 3079 Fragments arranged by size: 3079 988 852 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5446 HpaI GTT'AAC Cuts at: 3036 3036 Size: 5446 KpnI G_GTAC'C Cuts at: 2771 2771 Size: 5446 MfeI C'AATT_G Cuts at: 3045 3045 Size: 5446 MluI A'CGCG_T Cuts at: 5322 5322 Size: 5446 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2262 4560 4744 1305 Size: 957 2298 184 2007 Fragments arranged by size: 2298 2007 957 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2417 2594 10 Size: 54 1425 343 585 177 2862 Fragments arranged by size: 2862 1425 585 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 3404 3763 3922 518 Size: 648 2238 359 159 2042 Fragments arranged by size: 2238 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2078 2862 3539 4687 4932 1606 Size: 472 784 677 1148 245 2120 Fragments arranged by size: 2120 1148 784 677 472 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3955 Fragments arranged by size: 3955 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5446 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5446 NotI GC'GGCC_GC Cuts at: 2893 2893 Size: 5446 NspI r_CATG'y Cuts at: 1132 1229 2500 2804 4349 1132 Size: 97 1271 304 1545 2229 Fragments arranged by size: 2229 1545 1271 304 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5446 PciI A'CATG_T Cuts at: 4345 4345 Size: 5446 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5446 PfoI T'CCnGG_A Cuts at: 2180 2180 Size: 5446 PmeI GTTT'AAAC Cuts at: 2750 2750 Size: 5446 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3515 3547 5028 5060 3515 Size: 32 1481 32 3901 Fragments arranged by size: 3901 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5446 PsiI TTA'TAA Cuts at: 3016 3016 Size: 5446 PstI C_TGCA'G Cuts at: 830 2221 2798 830 Size: 1391 577 3478 Fragments arranged by size: 3478 1391 577 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5446 SacII CC_GC'GG Cuts at: 2079 2079 Size: 5446 SalI G'TCGA_C Cuts at: 1936 2788 1936 Size: 852 4594 SapI GCTCTTCn'nnn_ Cuts at: 2182 2182 Size: 5446 SbfI CC_TGCA'GG Cuts at: 2798 2798 Size: 5446 ScaI AGT'ACT Cuts at: 3694 5406 3694 Size: 1712 3734 SfcI C'TryA_G Cuts at: 826 1045 2217 2464 2794 3929 4610 4801 826 Size: 219 1172 247 330 1135 681 191 1471 Fragments arranged by size: 1471 1172 1135 681 330 247 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5446 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5446 SmaI CCC'GGG Cuts at: 1872 2773 1872 Size: 901 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3550 4451 4713 820 Size: 197 926 1607 901 262 1553 Fragments arranged by size: 1607 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3237 493 Size: 2744 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5446 SphI G_CATG'C Cuts at: 1132 2804 1132 Size: 1672 3774 SspI AAT'ATT Cuts at: 5 52 3370 5 Size: 47 3318 2081 Fragments arranged by size: 3318 2081 47 StuI AGG'CCT Cuts at: 1517 2462 2526 1517 Size: 945 64 4437 Fragments arranged by size: 4437 945 64 StyI C'CwwG_G Cuts at: 513 1065 2004 2885 513 Size: 552 939 881 3074 Fragments arranged by size: 3074 939 881 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2522 3473 3490 3643 3828 4273 325 Size: 1255 942 951 17 153 185 445 1498 Fragments arranged by size: 1498 1255 951 942 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3692 5404 96 Size: 274 80 33 51 1443 1715 1712 138 Fragments arranged by size: 1715 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2276 2308 5406 5438 2276 Size: 32 3098 32 2284 Fragments arranged by size: 3098 2284 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5446 XbaI T'CTAG_A Cuts at: 2782 2782 Size: 5446 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5446 XmaI C'CCGG_G Cuts at: 1870 2771 1870 Size: 901 4545 XmnI GAAnn'nnTTC Cuts at: 3575 3575 Size: 5446 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3254 276 Size: 53 83 186 936 1720 2468 Fragments arranged by size: 2468 1720 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI XcmI