(Circular) (Six-base) MAPSORT of: pFN21AB4700.seq Check: 9614 from: 1 to: 7423 pFN21AB4700 7423 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5233 278 Size: 53 83 186 936 3697 2468 Fragments arranged by size: 3697 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3038 3192 4766 1937 Size: 1101 154 1574 4594 Fragments arranged by size: 4594 1574 1101 154 Acc65I G'GTAC_C Cuts at: 3542 4744 3542 Size: 1202 6221 AclI AA'CG_TT Cuts at: 1563 5550 5923 1563 Size: 3987 373 3063 Fragments arranged by size: 3987 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2045 2136 2393 5486 6870 7138 1632 Size: 413 91 257 3093 1384 268 1917 Fragments arranged by size: 3093 1917 1384 413 268 257 91 AfeI AGC'GCT Cuts at: 2025 2025 Size: 7423 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7226 AflIII A'CryG_T Cuts at: 1203 2961 4701 6322 7299 1203 Size: 1758 1740 1621 977 1327 Fragments arranged by size: 1758 1740 1621 1327 977 AgeI A'CCGG_T Cuts at: 4169 6254 4169 Size: 2085 5338 AhdI GACnn_n'nnGTC Cuts at: 6152 6152 Size: 7423 AleI CACnn'nnGTG Cuts at: 1166 4136 1166 Size: 2970 4453 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3856 1184 Size: 2672 4751 AlwNI CAG_nnn'CTG Cuts at: 2609 2834 5166 6738 2609 Size: 225 2332 1572 3294 Fragments arranged by size: 3294 2332 1572 225 ApaLI G'TGCA_C Cuts at: 5480 6636 5480 Size: 1156 6267 ApoI r'AATT_y Cuts at: 1493 3423 3747 4010 4732 4958 5110 7219 1493 Size: 1930 324 263 722 226 152 2109 1697 Fragments arranged by size: 2109 1930 1697 722 324 263 226 152 AseI AT'TA_AT Cuts at: 160 1032 2730 3798 3867 4512 5977 160 Size: 872 1698 1068 69 645 1465 1606 Fragments arranged by size: 1698 1606 1465 1068 872 645 69 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7423 AvaI C'yCGr_G Cuts at: 1870 1943 2650 4748 1870 Size: 73 707 2098 4545 Fragments arranged by size: 4545 2098 707 73 BamHI G'GATC_C Cuts at: 4753 5120 4753 Size: 367 7056 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3542 4166 4172 4744 6199 Size: 325 217 627 1755 624 6 572 1455 Cuts at: 6199 618 Size: 1842 Fragments arranged by size: 1842 1755 1455 627 624 572 325 217 6 BanII G_rGCy'C Cuts at: 1391 4458 4619 4742 1391 Size: 3067 161 123 4072 Fragments arranged by size: 4072 3067 161 123 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4353 928 Size: 971 2454 3998 Fragments arranged by size: 3998 2454 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2121 2131 2155 2165 2879 2913 5614 Size: 34 184 10 24 10 714 34 2701 Cuts at: 5614 5648 1903 Size: 34 3678 Fragments arranged by size: 3678 2701 714 184 34 34 34 24 10 10 BciVI GTATCCnnnnn_n' Cuts at: 4473 5315 6525 4473 Size: 842 1210 5371 Fragments arranged by size: 5371 1210 842 BclI T'GATC_A Cuts at: 1548 1548 Size: 7423 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6034 136 Size: 107 122 71 631 605 4362 1525 Fragments arranged by size: 4362 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3780 7418 3780 Size: 3638 3785 BlpI GC'TnA_GC Cuts at: 3990 4840 3990 Size: 850 6573 Bme1580I G_kGCm'C Cuts at: 1790 2716 5484 6640 1790 Size: 926 2768 1156 2573 Fragments arranged by size: 2768 2573 1156 926 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7423 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2494 2607 4453 Size: 416 53 83 399 24 1077 113 1846 Cuts at: 4453 4681 5154 6112 442 Size: 228 473 958 1753 Fragments arranged by size: 1846 1753 1077 958 473 416 399 228 113 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7423 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2503 3126 3834 3921 4512 6083 1239 Size: 1264 623 708 87 591 1571 2579 Fragments arranged by size: 2579 1571 1264 708 623 591 87 Bpu10I CC'TnA_GC Cuts at: 3567 3567 Size: 7423 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2520 3798 3810 4384 4615 4633 5548 6413 6711 Size: 1278 12 574 231 18 915 865 298 Cuts at: 6711 2520 Size: 3232 Fragments arranged by size: 3232 1278 915 865 574 298 231 18 12 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2327 6086 882 Size: 617 828 3759 2219 Fragments arranged by size: 3759 2219 828 617 BsaAI yAC'GTr Cuts at: 493 1204 5214 493 Size: 711 4010 2702 Fragments arranged by size: 4010 2702 711 BsaBI GATnn'nnATC Cuts at: 3228 7417 3228 Size: 4189 3234 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2066 5230 5612 6247 Size: 53 83 186 936 533 3164 382 635 Cuts at: 6247 275 Size: 1451 Fragments arranged by size: 3164 1451 936 635 533 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4169 4465 5856 6254 6528 6675 7086 4169 Size: 296 1391 398 274 147 411 4506 Fragments arranged by size: 4506 1391 411 398 296 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7198 Fragments arranged by size: 7198 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2405 4353 1186 Size: 1219 1948 4256 Fragments arranged by size: 4256 1948 1219 BseYI C'CCAG_C Cuts at: 4604 6626 7122 4604 Size: 2022 496 4905 Fragments arranged by size: 4905 2022 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2151 4114 1163 Size: 573 415 1963 4472 Fragments arranged by size: 4472 1963 573 415 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3108 4873 5634 6662 1540 Size: 275 186 1107 1765 761 1028 2301 Fragments arranged by size: 2301 1765 1107 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4619 5484 5569 6640 4619 Size: 865 85 1071 5402 Fragments arranged by size: 5402 1071 865 85 BsmI GAATG_Cn' Cuts at: 4934 5027 4934 Size: 93 7330 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2716 4458 4619 4742 5484 5569 6640 Size: 399 926 1742 161 123 742 85 1071 Cuts at: 6640 1391 Size: 2174 Fragments arranged by size: 2174 1742 1071 926 742 399 161 123 85 BspEI T'CCGG_A Cuts at: 7086 7086 Size: 7423 BspHI T'CATG_A Cuts at: 5312 7042 5312 Size: 1730 5693 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4778 844 Size: 3934 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5310 1394 Size: 429 3487 3507 Fragments arranged by size: 3507 3487 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2200 4179 5918 6092 65 Size: 1183 952 1979 1739 174 1396 Fragments arranged by size: 1979 1739 1396 1183 952 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3539 4169 6067 6254 1700 Size: 110 1729 630 1898 187 2869 Fragments arranged by size: 2869 1898 1729 630 187 110 BsrGI T'GTAC_A Cuts at: 96 3261 96 Size: 3165 4258 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7423 BssSI C'ACGA_G Cuts at: 5483 6495 5483 Size: 1012 6411 BstAPI GCAn_nnn'nTGC Cuts at: 794 2882 794 Size: 2088 5335 BstBI TT'CG_AA Cuts at: 6227 6227 Size: 7423 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7423 BstYI r'GATC_y Cuts at: 1972 3280 3643 3780 4753 5120 5505 5522 6974 Size: 1308 363 137 973 367 385 17 1452 Cuts at: 6974 7060 7072 7418 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1308 973 385 367 363 346 137 86 17 12 BstZ17I GTA'TAC Cuts at: 3039 3039 Size: 7423 Bsu36I CC'TnA_GG Cuts at: 2835 2835 Size: 7423 BtgI C'CryG_G Cuts at: 513 1065 3102 3339 4679 513 Size: 552 2037 237 1340 3257 Fragments arranged by size: 3257 2037 1340 552 237 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6684 Fragments arranged by size: 6684 683 56 BtsI GCAGTG_nn' Cuts at: 2230 2579 3264 3561 4597 4599 4939 5733 5753 Size: 349 685 297 1036 2 340 794 20 Cuts at: 5753 2230 Size: 3900 Fragments arranged by size: 3900 1036 794 685 349 340 297 20 2 ClaI AT'CG_AT Cuts at: 1554 3580 1554 Size: 2026 5397 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7388 DraI TTT'AAA Cuts at: 4002 4317 4727 4002 Size: 315 410 6698 Fragments arranged by size: 6698 410 315 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3332 6430 809 Size: 507 2016 3098 1802 Fragments arranged by size: 3098 2016 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2458 3105 4870 Size: 54 1425 50 275 18 628 647 1765 Cuts at: 4870 5759 7388 8 Size: 889 1629 43 Fragments arranged by size: 1765 1629 1425 889 647 628 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3105 4870 1537 Size: 275 1293 1765 4090 Fragments arranged by size: 4090 1765 1293 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2172 2296 2471 5353 1353 Size: 819 124 175 2882 3423 Fragments arranged by size: 3423 2882 819 175 124 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3291 6010 6384 6530 387 Size: 1064 138 1702 2719 374 146 1280 Fragments arranged by size: 2719 1702 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4617 4617 Size: 7423 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2045 2136 2393 2503 3126 3834 3921 Size: 393 413 91 257 110 623 708 87 Cuts at: 3921 4512 5486 6083 6870 7138 1239 Size: 591 974 597 787 268 1524 Fragments arranged by size: 1524 974 787 708 623 597 591 413 393 268 257 110 91 87 EcoNI CCTnn'n_nnAGG Cuts at: 2060 2060 Size: 7423 EcoO109I rG'GnC_Cy Cuts at: 2525 3571 4519 4608 2525 Size: 1046 948 89 5340 Fragments arranged by size: 5340 1046 948 89 EcoRI G'AATT_C Cuts at: 3423 3747 4732 5110 3423 Size: 324 985 378 5736 Fragments arranged by size: 5736 985 378 324 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2398 2967 3548 2398 Size: 569 581 6273 Fragments arranged by size: 6273 581 569 FspI TGC'GCA Cuts at: 5929 5929 Size: 7423 HaeII r_GCGC'y Cuts at: 1678 2027 6570 1678 Size: 349 4543 2531 Fragments arranged by size: 4543 2531 349 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2472 2504 3674 3706 3952 Size: 32 135 32 900 32 1170 32 246 Cuts at: 3952 3984 6064 6096 6138 6170 1373 Size: 32 2080 32 42 32 2626 Fragments arranged by size: 2626 2080 1170 900 246 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3444 3552 4767 5013 669 Size: 988 281 1506 108 1215 246 3079 Fragments arranged by size: 3079 1506 1215 988 281 246 108 HindIII A'AGCT_T Cuts at: 748 2278 748 Size: 1530 5893 HpaI GTT'AAC Cuts at: 5013 5013 Size: 7423 KpnI G_GTAC'C Cuts at: 3546 4748 3546 Size: 1202 6221 MfeI C'AATT_G Cuts at: 2877 3628 3669 5022 2877 Size: 751 41 1353 5278 Fragments arranged by size: 5278 1353 751 41 MluI A'CGCG_T Cuts at: 7299 7299 Size: 7423 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2645 4143 4447 6537 6721 1305 Size: 1340 1498 304 2090 184 2007 Fragments arranged by size: 2090 2007 1498 1340 304 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2460 10 Size: 54 1425 343 628 4973 Fragments arranged by size: 4973 1425 628 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2999 3116 4136 5381 5740 5899 518 Size: 648 1833 117 1020 1245 359 159 2042 Fragments arranged by size: 2042 1833 1245 1020 648 359 159 117 MspA1I CmG'CkG Cuts at: 1606 2044 2659 3008 4372 4839 5516 6664 6909 Size: 438 615 349 1364 467 677 1148 245 Cuts at: 6909 1606 Size: 2120 Fragments arranged by size: 2120 1364 1148 677 615 467 438 349 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6871 NdeI CA'TA_TG Cuts at: 387 2857 3082 4558 387 Size: 2470 225 1476 3252 Fragments arranged by size: 3252 2470 1476 225 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7423 NotI GC'GGCC_GC Cuts at: 4870 4870 Size: 7423 NruI TCG'CGA Cuts at: 2685 2685 Size: 7423 NspI r_CATG'y Cuts at: 1132 1229 2303 2720 2965 4705 4781 6326 1132 Size: 97 1074 417 245 1740 76 1545 2229 Fragments arranged by size: 2229 1740 1545 1074 417 245 97 76 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7423 PciI A'CATG_T Cuts at: 2961 4701 6322 2961 Size: 1740 1621 4062 Fragments arranged by size: 4062 1740 1621 PflMI CCAn_nnn'nTGG Cuts at: 1108 3905 4490 1108 Size: 2797 585 4041 Fragments arranged by size: 4041 2797 585 PmeI GTTT'AAAC Cuts at: 4727 4727 Size: 7423 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5492 5524 7005 7037 5492 Size: 32 1481 32 5878 Fragments arranged by size: 5878 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2525 3571 4608 2525 Size: 1046 1037 5340 Fragments arranged by size: 5340 1046 1037 PshAI GACnn'nnGTC Cuts at: 1613 3125 1613 Size: 1512 5911 PsiI TTA'TAA Cuts at: 4993 4993 Size: 7423 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3245 3277 3245 Size: 32 7391 PstI C_TGCA'G Cuts at: 830 2236 3397 3780 4377 4480 4775 830 Size: 1406 1161 383 597 103 295 3478 Fragments arranged by size: 3478 1406 1161 597 383 295 103 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7423 PvuII CAG'CTG Cuts at: 2659 3008 4372 2659 Size: 349 1364 5710 Fragments arranged by size: 5710 1364 349 RsrII CG'GwC_CG Cuts at: 4468 4468 Size: 7423 SacI G_AGCT'C Cuts at: 4619 4619 Size: 7423 SalI G'TCGA_C Cuts at: 1936 4765 1936 Size: 2829 4594 SbfI CC_TGCA'GG Cuts at: 4775 4775 Size: 7423 ScaI AGT'ACT Cuts at: 5671 7383 5671 Size: 1712 5711 SexAI A'CCwGG_T Cuts at: 2811 2811 Size: 7423 SfcI C'TryA_G Cuts at: 826 1045 2070 2232 3057 3393 3776 4373 4476 Size: 219 1025 162 825 336 383 597 103 Cuts at: 4476 4771 5906 6587 6778 826 Size: 295 1135 681 191 1471 Fragments arranged by size: 1471 1135 1025 825 681 597 383 336 295 219 191 162 103 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7423 SgrAI Cr'CCGG_yG Cuts at: 1700 4169 1700 Size: 2469 4954 SmaI CCC'GGG Cuts at: 1872 4750 1872 Size: 2878 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2535 3813 3825 4363 4612 4630 Size: 197 926 592 1278 12 538 249 18 Cuts at: 4630 5527 6428 6690 820 Size: 897 901 262 1553 Fragments arranged by size: 1553 1278 926 901 897 592 538 262 249 197 18 12 SnaBI TAC'GTA Cuts at: 493 5214 493 Size: 4721 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7423 SphI G_CATG'C Cuts at: 1132 2303 4781 1132 Size: 1171 2478 3774 Fragments arranged by size: 3774 2478 1171 SspI AAT'ATT Cuts at: 5 52 3598 3871 4081 4312 5347 5 Size: 47 3546 273 210 231 1035 2081 Fragments arranged by size: 3546 2081 1035 273 231 210 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7423 StyI C'CwwG_G Cuts at: 513 1065 3417 4862 513 Size: 552 2352 1445 3074 Fragments arranged by size: 3074 2352 1445 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2971 3236 3323 3699 4142 5450 5467 Size: 1255 1391 265 87 376 443 1308 17 Cuts at: 5467 5620 5805 6250 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1391 1308 1255 445 443 376 265 185 153 87 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2209 3261 5669 Size: 274 80 33 51 1443 232 1052 2408 Cuts at: 5669 7381 96 Size: 1712 138 Fragments arranged by size: 2408 1712 1443 1052 274 232 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2727 2759 2844 2876 4138 4170 7383 7415 2727 Size: 32 85 32 1262 32 3213 32 2735 Fragments arranged by size: 3213 2735 1262 85 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2486 2762 1316 Size: 1170 276 5977 Fragments arranged by size: 5977 1170 276 XbaI T'CTAG_A Cuts at: 4759 4759 Size: 7423 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2621 2819 4411 2621 Size: 198 1592 5633 Fragments arranged by size: 5633 1592 198 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7423 XmaI C'CCGG_G Cuts at: 1870 4748 1870 Size: 2878 4545 XmnI GAAnn'nnTTC Cuts at: 3531 5552 3531 Size: 2021 5402 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5231 276 Size: 53 83 186 936 3697 2468 Fragments arranged by size: 3697 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII RsrII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PspOMI PspXI SacII SanDI SapI SfiI SfoI SrfI SwaI