Restriction map for pFN21AB4700
 (Linear) (Six-base) MAPSORT of: KIBB4700.seq  Check: 8641  from: 1  to: 2718



KIBB4700.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AccI GT'mk_AC

Cuts at:      0    1033    1187    2718 
   Size:       1033     154    1531

  Fragments arranged by size: 

                1531    1033     154
 
Acc65I G'GTAC_C

Cuts at:      0    1537    2718 
   Size:       1537    1181
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     131     388    2718 
   Size:         40      91     257    2330

  Fragments arranged by size: 

                2330     257      91      40
 
AfeI AGC'GCT

Cuts at:      0      20    2718 
   Size:         20    2698
 
AflIII A'CryG_T

Cuts at:      0     956    2696    2718 
   Size:        956    1740      22

  Fragments arranged by size: 

                1740     956      22
 
AgeI A'CCGG_T

Cuts at:      0    2164    2718 
   Size:       2164     554
 
AleI CACnn'nnGTG

Cuts at:      0    2131    2718 
   Size:       2131     587
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1851    2718 
   Size:       1851     867
 
AlwNI CAG_nnn'CTG

Cuts at:      0     604     829    2718 
   Size:        604     225    1889

  Fragments arranged by size: 

                1889     604     225
 
ApoI r'AATT_y

Cuts at:      0    1418    1742    2005    2718 
   Size:       1418     324     263     713

  Fragments arranged by size: 

                1418     713     324     263
 
AseI AT'TA_AT

Cuts at:      0     725    1793    1862    2507    2718 
   Size:        725    1068      69     645     211

  Fragments arranged by size: 

                1068     725     645     211      69
 
AvaI C'yCGr_G

Cuts at:      0     645    2718 
   Size:        645    2073
 
BanI G'GyrC_C

Cuts at:      0    1537    2161    2167    2718 
   Size:       1537     624       6     551

  Fragments arranged by size: 

                1537     624     551       6
 
BanII G_rGCy'C

Cuts at:      0    2453    2614    2718 
   Size:       2453     161     104

  Fragments arranged by size: 

                2453     161     104
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    2348    2718 
   Size:       2348     370
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     116     126     150     160     874     908    2718 
   Size:        116      10      24      10     714      34    1810

  Fragments arranged by size: 

                1810     714     116      34      24      10      10
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    2468    2718 
   Size:       2468     250
 
BglII A'GATC_T

Cuts at:      0    1775    2718 
   Size:       1775     943
 
BlpI GC'TnA_GC

Cuts at:      0    1985    2718 
   Size:       1985     733
 
Bme1580I G_kGCm'C

Cuts at:      0     711    2718 
   Size:        711    2007
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     489     602    2448    2676    2718 
   Size:        489     113    1846     228      42

  Fragments arranged by size: 

                1846     489     228     113      42
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     498    1121    1829    1916    2507    2718 
   Size:        498     623     708      87     591     211

  Fragments arranged by size: 

                 708     623     591     498     211      87
 
Bpu10I CC'TnA_GC

Cuts at:      0    1562    2718 
   Size:       1562    1156
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     515    1793    1805    2379    2610    2628    2718 
   Size:        515    1278      12     574     231      18      90

  Fragments arranged by size: 

                1278     574     515     231      90      18      12
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     322    2718 
   Size:        322    2396
 
BsaBI GATnn'nnATC

Cuts at:      0    1223    2718 
   Size:       1223    1495
 
BsaHI Gr'CG_yC

Cuts at:      0      61    2718 
   Size:         61    2657
 
BsaWI w'CCGG_w

Cuts at:      0    2164    2460    2718 
   Size:       2164     296     258

  Fragments arranged by size: 

                2164     296     258
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     400    2348    2718 
   Size:        400    1948     370

  Fragments arranged by size: 

                1948     400     370
 
BseYI C'CCAG_C

Cuts at:      0    2599    2718 
   Size:       2599     119
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146    2109    2718 
   Size:        146    1963     609

  Fragments arranged by size: 

                1963     609     146
 
BsiEI CG_ry'CG

Cuts at:      0    1103    2718 
   Size:       1103    1615
 
BsiHKAI G_wGCw'C

Cuts at:      0    2614    2718 
   Size:       2614     104
 
Bsp1286I G_dGCh'C

Cuts at:      0     711    2453    2614    2718 
   Size:        711    1742     161     104

  Fragments arranged by size: 

                1742     711     161     104
 
BsrDI GCAATG_nn'

Cuts at:      0     195    2174    2718 
   Size:        195    1979     544

  Fragments arranged by size: 

                1979     544     195
 
BsrFI r'CCGG_y

Cuts at:      0    1534    2164    2718 
   Size:       1534     630     554

  Fragments arranged by size: 

                1534     630     554
 
BsrGI T'GTAC_A

Cuts at:      0    1256    2718 
   Size:       1256    1462
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     877    2718 
   Size:        877    1841
 
BstYI r'GATC_y

Cuts at:      0    1275    1638    1775    2718 
   Size:       1275     363     137     943

  Fragments arranged by size: 

                1275     943     363     137
 
BstZ17I GTA'TAC

Cuts at:      0    1034    2718 
   Size:       1034    1684
 
Bsu36I CC'TnA_GG

Cuts at:      0     830    2718 
   Size:        830    1888
 
BtgI C'CryG_G

Cuts at:      0    1097    1334    2674    2718 
   Size:       1097     237    1340      44

  Fragments arranged by size: 

                1340    1097     237      44
 
BtsI GCAGTG_nn'

Cuts at:      0     225     574    1259    1556    2592    2594    2718 
   Size:        225     349     685     297    1036       2     124

  Fragments arranged by size: 

                1036     685     349     297     225     124       2
 
ClaI AT'CG_AT

Cuts at:      0    1575    2718 
   Size:       1575    1143
 
DraI TTT'AAA

Cuts at:      0    1997    2312    2718 
   Size:       1997     315     406

  Fragments arranged by size: 

                1997     406     315
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1327    2718 
   Size:       1327    1391
 
EaeI y'GGCC_r

Cuts at:      0     453    1100    2718 
   Size:        453     647    1618

  Fragments arranged by size: 

                1618     647     453
 
EagI C'GGCC_G

Cuts at:      0    1100    2718 
   Size:       1100    1618
 
EarI CTCTTCn'nnn_

Cuts at:      0     167     291     466    2718 
   Size:        167     124     175    2252

  Fragments arranged by size: 

                2252     175     167     124
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1286    2718 
   Size:       1286    1432
 
EcoICRI GAG'CTC

Cuts at:      0    2612    2718 
   Size:       2612     106
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     131     388     498    1121    1829    1916    2507 
   Size:         40      91     257     110     623     708      87     591

Cuts at:   2507    2718 
   Size:        211

  Fragments arranged by size: 

                 708     623     591     257     211     110      91      87
                  40
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      55    2718 
   Size:         55    2663
 
EcoO109I rG'GnC_Cy

Cuts at:      0     520    1566    2514    2603    2718 
   Size:        520    1046     948      89     115

  Fragments arranged by size: 

                1046     948     520     115      89
 
EcoRI G'AATT_C

Cuts at:      0    1418    1742    2718 
   Size:       1418     324     976

  Fragments arranged by size: 

                1418     976     324
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     393     962    1543    2718 
   Size:        393     569     581    1175

  Fragments arranged by size: 

                1175     581     569     393
 
HaeII r_GCGC'y

Cuts at:      0      22    2718 
   Size:         22    2696
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     467     499    1669    1701    1947    1979    2718 
   Size:        467      32    1170      32     246      32     739

  Fragments arranged by size: 

                1170     739     467     246      32      32      32
 
HincII GTy'rAC

Cuts at:      0    1439    1547    2718 
   Size:       1439     108    1171

  Fragments arranged by size: 

                1439    1171     108
 
HindIII A'AGCT_T

Cuts at:      0     273    2718 
   Size:        273    2445
 
KpnI G_GTAC'C

Cuts at:      0    1541    2718 
   Size:       1541    1177
 
MfeI C'AATT_G

Cuts at:      0     872    1623    1664    2718 
   Size:        872     751      41    1054

  Fragments arranged by size: 

                1054     872     751      41
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     640    2138    2442    2718 
   Size:        640    1498     304     276

  Fragments arranged by size: 

                1498     640     304     276
 
MscI TGG'CCA

Cuts at:      0     455    2718 
   Size:        455    2263
 
MslI CAynn'nnrTG

Cuts at:      0     994    1111    2131    2718 
   Size:        994     117    1020     587

  Fragments arranged by size: 

                1020     994     587     117
 
MspA1I CmG'CkG

Cuts at:      0      39     654    1003    2367    2718 
   Size:         39     615     349    1364     351

  Fragments arranged by size: 

                1364     615     351     349      39
 
NdeI CA'TA_TG

Cuts at:      0     852    1077    2553    2718 
   Size:        852     225    1476     165

  Fragments arranged by size: 

                1476     852     225     165
 
NruI TCG'CGA

Cuts at:      0     680    2718 
   Size:        680    2038
 
NspI r_CATG'y

Cuts at:      0     298     715     960    2700    2718 
   Size:        298     417     245    1740      18

  Fragments arranged by size: 

                1740     417     298     245      18
 
PciI A'CATG_T

Cuts at:      0     956    2696    2718 
   Size:        956    1740      22

  Fragments arranged by size: 

                1740     956      22
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1900    2485    2718 
   Size:       1900     585     233

  Fragments arranged by size: 

                1900     585     233
 
PpuMI rG'GwC_Cy

Cuts at:      0     520    1566    2603    2718 
   Size:        520    1046    1037     115

  Fragments arranged by size: 

                1046    1037     520     115
 
PshAI GACnn'nnGTC

Cuts at:      0    1120    2718 
   Size:       1120    1598
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1240    1272    2718 
   Size:       1240      32    1446

  Fragments arranged by size: 

                1446    1240      32
 
PstI C_TGCA'G

Cuts at:      0     231    1392    1775    2372    2475    2718 
   Size:        231    1161     383     597     103     243

  Fragments arranged by size: 

                1161     597     383     243     231     103
 
PvuII CAG'CTG

Cuts at:      0     654    1003    2367    2718 
   Size:        654     349    1364     351

  Fragments arranged by size: 

                1364     654     351     349
 
RsrII CG'GwC_CG

Cuts at:      0    2463    2718 
   Size:       2463     255
 
SacI G_AGCT'C

Cuts at:      0    2614    2718 
   Size:       2614     104
 
SexAI A'CCwGG_T

Cuts at:      0     806    2718 
   Size:        806    1912
 
SfcI C'TryA_G

Cuts at:      0      65     227    1052    1388    1771    2368    2471    2718 
   Size:         65     162     825     336     383     597     103     247

  Fragments arranged by size: 

                 825     597     383     336     247     162     103      65
            
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2164    2718 
   Size:       2164     554
 
SmlI C'TyrA_G

Cuts at:      0     530    1808    1820    2358    2607    2625    2718 
   Size:        530    1278      12     538     249      18      93

  Fragments arranged by size: 

                1278     538     530     249      93      18      12
 
SphI G_CATG'C

Cuts at:      0     298    2718 
   Size:        298    2420
 
SspI AAT'ATT

Cuts at:      0    1593    1866    2076    2307    2718 
   Size:       1593     273     210     231     411

  Fragments arranged by size: 

                1593     411     273     231     210
 
StyI C'CwwG_G

Cuts at:      0    1412    2718 
   Size:       1412    1306
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     966    1231    1318    1694    2137    2718 
   Size:        966     265      87     376     443     581

  Fragments arranged by size: 

                 966     581     443     376     265      87
 
TatI w'GTAC_w

Cuts at:      0     204    1256    2718 
   Size:        204    1052    1462

  Fragments arranged by size: 

                1462    1052     204
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     722     754     839     871    2133    2165    2718 
   Size:        722      32      85      32    1262      32     553

  Fragments arranged by size: 

                1262     722     553      85      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     481     757    2718 
   Size:        481     276    1961

  Fragments arranged by size: 

                1961     481     276
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     616     814    2406    2718 
   Size:        616     198    1592     312

  Fragments arranged by size: 

                1592     616     312     198
 
XmnI GAAnn'nnTTC

Cuts at:      0    1526    2718 
   Size:       1526    1192
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AfeI   AflIII     AgeI     AleI     AlfI
    AlwNI     ApoI     AseI     AvaI     BanI    BanII     BbsI     BcgI
    BciVI    BglII     BlpI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BsrDI    BsrFI    BsrGI   BstAPI    BstYI  BstZ17I
   Bsu36I     BtgI     BtsI     ClaI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
    HaeII    Hin4I   HincII  HindIII     KpnI     MfeI     MmeI     MscI
     MslI   MspA1I     NdeI     NruI     NspI     PciI    PflMI    PpuMI
    PshAI     PsrI     PstI    PvuII    RsrII     SacI    SexAI     SfcI
    SgrAI     SmlI     SphI     SspI     StyI    TaqII     TatI     TstI
  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI    AflII     AhdI     AloI     ApaI    ApaLI
     AscI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BclI
    BfrBI     BglI    BmgBI     BmtI     BplI    BsaAI    BsaXI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI   BssHII    BssSI
    BstBI   BstEII    BstXI    BtgZI    CspCI   DraIII    EcoRV     FseI
     FspI    FspAI     HpaI     KasI     MluI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NsiI     PacI     PasI     PfoI     PmeI
     PmlI     PpiI     PsiI   PspOMI    PspXI     PvuI    SacII     SalI
    SanDI     SapI     SbfI     ScaI     SfiI     SfoI     SgfI     SmaI
    SnaBI     SpeI     SrfI     StuI     SwaI     XbaI     XhoI     XmaI
     ZraI
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