Restriction map for pFN21AB4679
 (Circular) (Six-base) MAPSORT of: pFN21AB4679.seq  Check: 3036  from: 1  to: 5308



pFN21AB4679  5308 bp

 With 182 enzymes: SgfI * 

                        October 20, 2009 10:40  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2426    2426 
   Size:       5308
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3118     278 
   Size:         53      83     186     936    1582    2468

  Fragments arranged by size: 

                2468    1582     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2651    1937 
   Size:        714    4594
 
Acc65I G'GTAC_C

Cuts at:   2247    2629    2247 
   Size:        382    4926
 
AclI AA'CG_TT

Cuts at:   1563    3435    3808    1563 
   Size:       1872     373    3063

  Fragments arranged by size: 

                3063    1872     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3371    4755    5023    1632 
   Size:       1739    1384     268    1917

  Fragments arranged by size: 

                1917    1739    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5111
 
AflIII A'CryG_T

Cuts at:   1203    2106    2567    4207    5184    1203 
   Size:        903     461    1640     977    1327

  Fragments arranged by size: 

                1640    1327     977     903     461
 
AgeI A'CCGG_T

Cuts at:   4139    4139 
   Size:       5308
 
AhdI GACnn_n'nnGTC

Cuts at:   4037    4037 
   Size:       5308
 
AleI CACnn'nnGTG

Cuts at:   1166    2427    1166 
   Size:       1261    4047
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5308
 
AlwNI CAG_nnn'CTG

Cuts at:   3051    4623    3051 
   Size:       1572    3736
 
ApaLI G'TGCA_C

Cuts at:   3365    4521    3365 
   Size:       1156    4152
 
ApoI r'AATT_y

Cuts at:   1493    2617    2843    2995    5104    1493 
   Size:       1124     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1124     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3862     160 
   Size:        872    2830    1606

  Fragments arranged by size: 

                2830    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5308
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2328    2633    1870 
   Size:         73     385     305    4545

  Fragments arranged by size: 

                4545     385     305      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2588    2621    2588 
   Size:         33    5275
 
BamHI G'GATC_C

Cuts at:   2638    3005    2638 
   Size:        367    4941
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2247    2629    4084     618 
   Size:        325     217     627     460     382    1455    1842

  Fragments arranged by size: 

                1842    1455     627     460     382     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2326    2627    1391 
   Size:        935     301    4072

  Fragments arranged by size: 

                4072     935     301
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2214     928 
   Size:        971     315    4022

  Fragments arranged by size: 

                4022     971     315
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2461    2495    3499    3533    1903 
   Size:         34     524      34    1004      34    3678

  Fragments arranged by size: 

                3678    1004     524      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2589    3200    4410    2589 
   Size:        611    1210    3487

  Fragments arranged by size: 

                3487    1210     611
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5308
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2298    3919     136 
   Size:        107     122      71     631     605     626    1621    1525

  Fragments arranged by size: 

                1621    1525     631     626     605     122     107      71
            
 
BglII A'GATC_T

Cuts at:   2546    5303    2546 
   Size:       2757    2551
 
BlpI GC'TnA_GC

Cuts at:   2725    2725 
   Size:       5308
 
Bme1580I G_kGCm'C

Cuts at:   1790    3369    4525    1790 
   Size:       1579    1156    2573

  Fragments arranged by size: 

                2573    1579    1156
 
BmgBI CAC'GTC

Cuts at:   1318    2107    1318 
   Size:        789    4519
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3039    3997     442 
   Size:        416      53      83     399      24    1622     958    1753

  Fragments arranged by size: 

                1753    1622     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5308
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2061    3968    1239 
   Size:        822    1907    2579

  Fragments arranged by size: 

                2579    1907     822
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2129    3433    4298    4596    2129 
   Size:       1304     865     298    2841

  Fragments arranged by size: 

                2841    1304     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3971     882 
   Size:        617    2472    2219

  Fragments arranged by size: 

                2472    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2062    2570    3099     493 
   Size:        711     858     508     529    2702

  Fragments arranged by size: 

                2702     858     711     529     508
 
BsaBI GATnn'nnATC

Cuts at:   5302    5302 
   Size:       5308
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3115    3497    4132     275 
   Size:         53      83     186     936    1582     382     635    1451

  Fragments arranged by size: 

                1582    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3741    4139    4413    4560    4971    3741 
   Size:        398     274     147     411    4078

  Fragments arranged by size: 

                4078     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2004    2034    1178 
   Size:         30     165      30     601      30    4452

  Fragments arranged by size: 

                4452     601     165      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       5308
 
BseYI C'CCAG_C

Cuts at:   4511    5007    4511 
   Size:        496    4812
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4735
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2281    2758    3519    4547    1540 
   Size:        275     186     280     477     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     477     280     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2326    3369    3454    4525    2326 
   Size:       1043      85    1071    3109

  Fragments arranged by size: 

                3109    1071    1043      85
 
BsmI GAATG_Cn'

Cuts at:   2819    2912    2819 
   Size:         93    5215
 
BsmBI CGTCTCn'nnnn_

Cuts at:   2193    2193 
   Size:       5308
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2326    2627    3369    3454    4525    1391 
   Size:        399     536     301     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     536     399     301      85
 
BspEI T'CCGG_A

Cuts at:   4971    4971 
   Size:       5308
 
BspHI T'CATG_A

Cuts at:   2551    3197    4927    2551 
   Size:        646    1730    2932

  Fragments arranged by size: 

                2932    1730     646
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2426    2663     844 
   Size:       1582     237    3489

  Fragments arranged by size: 

                3489    1582     237
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3195    1394 
   Size:        429    1372    3507

  Fragments arranged by size: 

                3507    1372     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3803    3977      65 
   Size:       1183    2555     174    1396

  Fragments arranged by size: 

                2555    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3952    4139    1700 
   Size:        110    2142     187    2869

  Fragments arranged by size: 

                2869    2142     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5308
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5308
 
BssSI C'ACGA_G

Cuts at:   3368    4380    3368 
   Size:       1012    4296
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5308
 
BstBI TT'CG_AA

Cuts at:   2521    4112    2521 
   Size:       1591    3717
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5308
 
BstYI r'GATC_y

Cuts at:   1972    2546    2638    3005    3390    3407    4859    4945    4957 
   Size:        574      92     367     385      17    1452      86      12

Cuts at:   4957    5303    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     574     385     367     346      92      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2155    2281     513 
   Size:        552    1090     126    3540

  Fragments arranged by size: 

                3540    1090     552     126
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4569

  Fragments arranged by size: 

                4569     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2824    3618    3638    2824 
   Size:        794      20    4494

  Fragments arranged by size: 

                4494     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5308
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5273
 
DraI TTT'AAA

Cuts at:   2415    2612    2415 
   Size:        197    5111
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4315     809 
   Size:        507    2999    1802

  Fragments arranged by size: 

                2999    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2278    2755    3644 
   Size:         54    1425      50     275      18     448     477     889

Cuts at:   3644    5273       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     477     448     275      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2278    2755    1537 
   Size:        275     466     477    4090

  Fragments arranged by size: 

                4090     477     466     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3238    1353 
   Size:       1885    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2334    3895    4269    4415     387 
   Size:       1064     138     745    1561     374     146    1280

  Fragments arranged by size: 

                1561    1280    1064     745     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2324    2324 
   Size:       5308
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2061    3371    3968    4755    5023    1239 
   Size:        393     429    1310     597     787     268    1524

  Fragments arranged by size: 

                1524    1310     787     597     429     393     268
 
EcoO109I rG'GnC_Cy

Cuts at:   2191    2191 
   Size:       5308
 
EcoRI G'AATT_C

Cuts at:   2617    2995    2617 
   Size:        378    4930
 
FspI TGC'GCA

Cuts at:   3814    3814 
   Size:       5308
 
HaeII r_GCGC'y

Cuts at:   1678    2127    4455    1678 
   Size:        449    2328    2531

  Fragments arranged by size: 

                2531    2328     449
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2361    2393    3949    3981    4023 
   Size:         32     135      32     789      32    1556      32      42

Cuts at:   4023    4055    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1556     789     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2652    2898     669 
   Size:        988     281     714     246    3079

  Fragments arranged by size: 

                3079     988     714     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5308
 
HpaI GTT'AAC

Cuts at:   2898    2898 
   Size:       5308
 
KpnI G_GTAC'C

Cuts at:   2251    2633    2251 
   Size:        382    4926
 
MfeI C'AATT_G

Cuts at:   2907    2907 
   Size:       5308
 
MluI A'CGCG_T

Cuts at:   5184    5184 
   Size:       5308
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4422    4606    1305 
   Size:       3117     184    2007

  Fragments arranged by size: 

                3117    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3486

  Fragments arranged by size: 

                3486    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2060    2427    3266    3625    3784     518 
   Size:        648     894     367     839     359     159    2042

  Fragments arranged by size: 

                2042     894     839     648     367     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2157    2724    3401    4549    4794    1606 
   Size:        551     567     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     567     551     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4756
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5308
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5308
 
NotI GC'GGCC_GC

Cuts at:   2755    2755 
   Size:       5308
 
NruI TCG'CGA

Cuts at:   2226    2226 
   Size:       5308
 
NspI r_CATG'y

Cuts at:   1132    1229    2428    2666    4211    1132 
   Size:         97    1199     238    1545    2229

  Fragments arranged by size: 

                2229    1545    1199     238      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5308
 
PciI A'CATG_T

Cuts at:   4207    4207 
   Size:       5308
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5308
 
PmeI GTTT'AAAC

Cuts at:   2612    2612 
   Size:       5308
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2093    2125    3377    3409    4890    4922    2093 
   Size:         32    1252      32    1481      32    2479

  Fragments arranged by size: 

                2479    1481    1252      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2191    2191 
   Size:       5308
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5308
 
PsiI TTA'TAA

Cuts at:   2878    2878 
   Size:       5308
 
PstI C_TGCA'G

Cuts at:    830    2660     830 
   Size:       1830    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5308
 
SacI G_AGCT'C

Cuts at:   2326    2326 
   Size:       5308
 
SacII CC_GC'GG

Cuts at:   2158    2158 
   Size:       5308
 
SalI G'TCGA_C

Cuts at:   1936    2650    1936 
   Size:        714    4594
 
SbfI CC_TGCA'GG

Cuts at:   2660    2660 
   Size:       5308
 
ScaI AGT'ACT

Cuts at:   3556    5268    3556 
   Size:       1712    3596
 
SfcI C'TryA_G

Cuts at:    826    1045    2656    3791    4472    4663     826 
   Size:        219    1611    1135     681     191    1471

  Fragments arranged by size: 

                1611    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5308
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5308
 
SmaI CCC'GGG

Cuts at:   1872    2635    1872 
   Size:        763    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2144    3412    4313    4575     820 
   Size:        197     926     201    1268     901     262    1553

  Fragments arranged by size: 

                1553    1268     926     901     262     201     197
 
SnaBI TAC'GTA

Cuts at:    493    3099     493 
   Size:       2606    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5308
 
SphI G_CATG'C

Cuts at:   1132    2666    1132 
   Size:       1534    3774
 
SspI AAT'ATT

Cuts at:      5      52    3232       5 
   Size:         47    3180    2081

  Fragments arranged by size: 

                3180    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5308
 
StyI C'CwwG_G

Cuts at:    513    1065    2091    2747     513 
   Size:        552    1026     656    3074

  Fragments arranged by size: 

                3074    1026     656     552
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3335    3352    3505    3690    4135     325 
   Size:       1255    1755      17     153     185     445    1498

  Fragments arranged by size: 

                1755    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3554    5266      96 
   Size:        274      80      33      51    1443    1577    1712     138

  Fragments arranged by size: 

                1712    1577    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2004    2036    5268    5300    2004 
   Size:         32    3232      32    2012

  Fragments arranged by size: 

                3232    2012      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5308
 
XbaI T'CTAG_A

Cuts at:   2644    2644 
   Size:       5308
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2400    2400 
   Size:       5308
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5308
 
XmaI C'CCGG_G

Cuts at:   1870    2633    1870 
   Size:        763    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3437    3437 
   Size:       5308
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3116     276 
   Size:         53      83     186     936    1582    2468

  Fragments arranged by size: 

                2468    1582     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbsI     BcgI
    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NruI     NspI
     PasI     PciI    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI
     PstI     PvuI     SacI    SacII     SalI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AloI     ApaI     AscI    AvrII     BbeI    BbvCI    BfrBI
     BplI   Bpu10I    BsiWI    BstXI  BstZ17I   Bsu36I   DraIII    EcoNI
    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI   NgoMIV
     NsiI     PacI     PfoI     PmlI   PspOMI    PspXI     PsrI    PvuII
    RsrII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
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