Restriction map for
pFN21AB4596
(Circular) (Six-base) MAPSORT of: pFN21AB4596.seq Check: 7311 from: 1 to: 7456
pFN21AB4596 7456 bp
With 182 enzymes: SgfI *
February 27, 2009 14:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4448 4448
Size: 7456
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5266 278
Size: 53 83 186 936 3730 2468
Fragments arranged by size:
3730 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3240 4799 1937
Size: 1303 1559 4594
Fragments arranged by size:
4594 1559 1303
Acc65I G'GTAC_C
Cuts at: 4777 4777
Size: 7456
AclI AA'CG_TT
Cuts at: 1563 5583 5956 1563
Size: 4020 373 3063
Fragments arranged by size:
4020 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2066 2116 4352 5519 6903 7171 1632
Size: 434 50 2236 1167 1384 268 1917
Fragments arranged by size:
2236 1917 1384 1167 434 268 50
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7259
AflIII A'CryG_T
Cuts at: 1203 4665 6355 7332 1203
Size: 3462 1690 977 1327
Fragments arranged by size:
3462 1690 1327 977
AgeI A'CCGG_T
Cuts at: 6287 6287
Size: 7456
AhdI GACnn_n'nnGTC
Cuts at: 6185 6185
Size: 7456
AleI CACnn'nnGTG
Cuts at: 1166 2367 4501 4721 1166
Size: 1201 2134 220 3901
Fragments arranged by size:
3901 2134 1201 220
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3537 3901 1184
Size: 2353 364 4739
Fragments arranged by size:
4739 2353 364
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2904 2936 2904
Size: 32 7424
AlwNI CAG_nnn'CTG
Cuts at: 2023 5199 6771 2023
Size: 3176 1572 2708
Fragments arranged by size:
3176 2708 1572
ApaLI G'TGCA_C
Cuts at: 2360 5513 6669 2360
Size: 3153 1156 3147
Fragments arranged by size:
3153 3147 1156
ApoI r'AATT_y
Cuts at: 1493 2277 3941 4765 4991 5143 7252 1493
Size: 784 1664 824 226 152 2109 1697
Fragments arranged by size:
2109 1697 1664 824 784 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6010 160
Size: 872 4978 1606
Fragments arranged by size:
4978 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7456
AvaI C'yCGr_G
Cuts at: 1870 1943 2160 2994 4338 4781 1870
Size: 73 217 834 1344 443 4545
Fragments arranged by size:
4545 1344 834 443 217 73
BamHI G'GATC_C
Cuts at: 2855 4786 5153 2855
Size: 1931 367 5158
Fragments arranged by size:
5158 1931 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2639 2702 3176 3307 3983
Size: 325 217 627 852 63 474 131 676
Cuts at: 3983 4777 6232 618
Size: 794 1455 1842
Fragments arranged by size:
1842 1455 852 794 676 627 474 325
217 131 63
BanII G_rGCy'C
Cuts at: 1391 4014 4775 1391
Size: 2623 761 4072
Fragments arranged by size:
4072 2623 761
BbeI G_GCGC'C
Cuts at: 3180 3180
Size: 7456
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2259 2823 4340 928
Size: 971 360 564 1517 4044
Fragments arranged by size:
4044 1517 971 564 360
BbvCI CC'TCA_GC
Cuts at: 3500 3500
Size: 7456
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5647 5681 1903
Size: 34 3710 34 3678
Fragments arranged by size:
3710 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4463 5348 6558 4463
Size: 885 1210 5361
Fragments arranged by size:
5361 1210 885
BclI T'GATC_A
Cuts at: 1548 2386 2508 2688 3730 3852 4236 1548
Size: 838 122 180 1042 122 384 4768
Fragments arranged by size:
4768 1042 838 384 180 122 122
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6067 136
Size: 107 122 71 631 605 4395 1525
Fragments arranged by size:
4395 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3678 4317 7451 3678
Size: 639 3134 3683
Fragments arranged by size:
3683 3134 639
BlpI GC'TnA_GC
Cuts at: 3148 4873 3148
Size: 1725 5731
Bme1580I G_kGCm'C
Cuts at: 1790 2364 2642 3212 3713 3986 5517 6673 1790
Size: 574 278 570 501 273 1531 1156 2573
Fragments arranged by size:
2573 1531 1156 574 570 501 278 273
BmgBI CAC'GTC
Cuts at: 1318 3955 1318
Size: 2637 4819
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3182 3723 5187
Size: 416 53 83 399 24 1765 541 1464
Cuts at: 5187 6145 442
Size: 958 1753
Fragments arranged by size:
1765 1753 1464 958 541 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7456
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3479 4382 4400 4603 6116 1239
Size: 2240 903 18 203 1513 2579
Fragments arranged by size:
2579 2240 1513 903 203 18
Bpu10I CC'TnA_GC
Cuts at: 3500 3500
Size: 7456
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5581 6446 6744 5581
Size: 865 298 6293
Fragments arranged by size:
6293 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3413 4346 6119 882
Size: 617 1914 933 1773 2219
Fragments arranged by size:
2219 1914 1773 933 617
BsaAI yAC'GTr
Cuts at: 493 1204 3858 5247 493
Size: 711 2654 1389 2702
Fragments arranged by size:
2702 2654 1389 711
BsaBI GATnn'nnATC
Cuts at: 2169 2771 4115 7450 2169
Size: 602 1344 3335 2175
Fragments arranged by size:
3335 2175 1344 602
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3177 5263 5645 6280
Size: 53 83 186 936 1644 2086 382 635
Cuts at: 6280 275
Size: 1451
Fragments arranged by size:
2086 1644 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2823 3552 5889 6287 6561 6708 7119 2823
Size: 729 2337 398 274 147 411 3160
Fragments arranged by size:
3160 2337 729 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2541 2571 2755 2785 2904
Size: 30 165 30 1138 30 184 30 119
Cuts at: 2904 2934 3725 3755 1178
Size: 30 791 30 4879
Fragments arranged by size:
4879 1138 791 184 165 119 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3323 3431 4022 4082 1186
Size: 2137 108 591 60 4560
Fragments arranged by size:
4560 2137 591 108 60
BseYI C'CCAG_C
Cuts at: 3493 4211 6659 7155 3493
Size: 718 2448 496 3794
Fragments arranged by size:
3794 2448 718 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6883
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4722 4906 5667 6695 1540
Size: 275 186 2721 184 761 1028 2301
Fragments arranged by size:
2721 2301 1028 761 275 186 184
BsiHKAI G_wGCw'C
Cuts at: 2364 5517 5602 6673 2364
Size: 3153 85 1071 3147
Fragments arranged by size:
3153 3147 1071 85
BsmI GAATG_Cn'
Cuts at: 4967 5060 4967
Size: 93 7363
BsmBI CGTCTCn'nnnn_
Cuts at: 2748 2793 4319 2748
Size: 45 1526 5885
Fragments arranged by size:
5885 1526 45
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2364 2642 3212 3713 3986 4014 4775
Size: 399 574 278 570 501 273 28 761
Cuts at: 4775 5517 5602 6673 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 761 742 574 570 501 399
278 273 85 28
BspEI T'CCGG_A
Cuts at: 2823 3552 7119 2823
Size: 729 3567 3160
Fragments arranged by size:
3567 3160 729
BspHI T'CATG_A
Cuts at: 2121 2299 5345 7075 2121
Size: 178 3046 1730 2502
Fragments arranged by size:
3046 2502 1730 178
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4448 4811 844
Size: 3604 363 3489
Fragments arranged by size:
3604 3489 363
BsrBI CCG'CTC
Cuts at: 1394 1823 2538 5343 1394
Size: 429 715 2805 3507
Fragments arranged by size:
3507 2805 715 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2244 4057 5951 6125 65
Size: 1183 996 1813 1894 174 1396
Fragments arranged by size:
1894 1813 1396 1183 996 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3405 4043 6100 6287 1700
Size: 110 1595 638 2057 187 2869
Fragments arranged by size:
2869 2057 1595 638 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7456
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7456
BssSI C'ACGA_G
Cuts at: 5516 6528 5516
Size: 1012 6444
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4056 794
Size: 3262 4194
BstBI TT'CG_AA
Cuts at: 2275 6260 2275
Size: 3985 3471
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7456
BstXI CCAn_nnnn'nTGG
Cuts at: 3160 3160
Size: 7456
BstYI r'GATC_y
Cuts at: 1972 2254 2855 3245 3598 3678 4317 4536 4786
Size: 282 601 390 353 80 639 219 250
Cuts at: 4786 5153 5538 5555 7007 7093 7105 7451 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 639 601 390 385 367 353
346 282 250 219 86 80 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3228 3366 3525 4722 513
Size: 552 2163 138 159 1197 3247
Fragments arranged by size:
3247 2163 1197 552 159 138
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4458 487
Size: 683 56 3232 3485
Fragments arranged by size:
3485 3232 683 56
BtsI GCAGTG_nn'
Cuts at: 2374 4391 4972 5766 5786 2374
Size: 2017 581 794 20 4044
Fragments arranged by size:
4044 2017 794 581 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7456
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7421
DraI TTT'AAA
Cuts at: 2530 4760 2530
Size: 2230 5226
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4560 6463 809
Size: 507 3244 1903 1802
Fragments arranged by size:
3244 1903 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2943 3393 4719
Size: 54 1425 50 275 18 1113 450 1326
Cuts at: 4719 4903 5792 7421 8
Size: 184 889 1629 43
Fragments arranged by size:
1629 1425 1326 1113 889 450 275 184
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4719 4903 1537
Size: 275 2907 184 4090
Fragments arranged by size:
4090 2907 275 184
EarI CTCTTCn'nnn_
Cuts at: 1353 3464 3714 4310 5386 1353
Size: 2111 250 596 1076 3423
Fragments arranged by size:
3423 2111 1076 596 250
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2986 6043 6417 6563 387
Size: 1064 138 1397 3057 374 146 1280
Fragments arranged by size:
3057 1397 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2116 3479 4352 4382 4400 4603
Size: 393 434 50 1363 873 30 18 203
Cuts at: 4603 5519 6116 6903 7171 1239
Size: 916 597 787 268 1524
Fragments arranged by size:
1524 1363 916 873 787 597 434 393
268 203 50 30 18
EcoNI CCTnn'n_nnAGG
Cuts at: 3913 4682 3913
Size: 769 6687
EcoO109I rG'GnC_Cy
Cuts at: 2953 3782 4134 2953
Size: 829 352 6275
Fragments arranged by size:
6275 829 352
EcoRI G'AATT_C
Cuts at: 2277 4765 5143 2277
Size: 2488 378 4590
Fragments arranged by size:
4590 2488 378
EcoRV GAT'ATC
Cuts at: 2119 4165 2119
Size: 2046 5410
FspI TGC'GCA
Cuts at: 5962 5962
Size: 7456
HaeII r_GCGC'y
Cuts at: 1678 3180 3304 6603 1678
Size: 1502 124 3299 2531
Fragments arranged by size:
3299 2531 1502 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2792 2824 2840 2872 3095
Size: 32 135 32 1220 32 16 32 223
Cuts at: 3095 3127 3412 3444 4302 4334 6097 6129 6171
Size: 32 285 32 858 32 1763 32 42
Cuts at: 6171 6203 1373
Size: 32 2626
Fragments arranged by size:
2626 1763 1220 858 285 223 135 42
32 32 32 32 32 32 32 32
32 16
HincII GTy'rAC
Cuts at: 669 1657 1938 3241 3970 4800 5046 669
Size: 988 281 1303 729 830 246 3079
Fragments arranged by size:
3079 1303 988 830 729 281 246
HindIII A'AGCT_T
Cuts at: 748 4601 748
Size: 3853 3603
HpaI GTT'AAC
Cuts at: 5046 5046
Size: 7456
KasI G'GCGC_C
Cuts at: 3176 3176
Size: 7456
KpnI G_GTAC'C
Cuts at: 4781 4781
Size: 7456
MfeI C'AATT_G
Cuts at: 5055 5055
Size: 7456
MluI A'CGCG_T
Cuts at: 7332 7332
Size: 7456
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2198 4006 6570 6754 1305
Size: 893 1808 2564 184 2007
Fragments arranged by size:
2564 2007 1808 893 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2945 10
Size: 54 1425 343 1113 4521
Fragments arranged by size:
4521 1425 1113 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2367 2645 3519 3989 4121 4501 4657
Size: 648 1201 278 874 470 132 380 156
Cuts at: 4657 4721 5414 5773 5932 518
Size: 64 693 359 159 2042
Fragments arranged by size:
2042 1201 874 693 648 470 380 359
278 159 156 132 64
MspA1I CmG'CkG
Cuts at: 1606 2878 3351 4501 4695 4872 5549 6697 6942
Size: 1272 473 1150 194 177 677 1148 245
Cuts at: 6942 1606
Size: 2120
Fragments arranged by size:
2120 1272 1150 1148 677 473 245 194
177
NarI GG'CG_CC
Cuts at: 3177 3177
Size: 7456
NcoI C'CATG_G
Cuts at: 513 1065 3366 513
Size: 552 2301 4603
Fragments arranged by size:
4603 2301 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7456
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7456
NotI GC'GGCC_GC
Cuts at: 4903 4903
Size: 7456
NspI r_CATG'y
Cuts at: 1132 1229 2905 3454 4814 6359 1132
Size: 97 1676 549 1360 1545 2229
Fragments arranged by size:
2229 1676 1545 1360 549 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7456
PciI A'CATG_T
Cuts at: 6355 6355
Size: 7456
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7456
PfoI T'CCnGG_A
Cuts at: 4484 4484
Size: 7456
PmeI GTTT'AAAC
Cuts at: 4760 4760
Size: 7456
PmlI CAC'GTG
Cuts at: 3858 3858
Size: 7456
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2904 2936 5525 5557 7038 7070 2904
Size: 32 2589 32 1481 32 3290
Fragments arranged by size:
3290 2589 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7456
PsiI TTA'TAA
Cuts at: 5026 5026
Size: 7456
PstI C_TGCA'G
Cuts at: 830 4808 830
Size: 3978 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7456
PvuII CAG'CTG
Cuts at: 2878 4501 2878
Size: 1623 5833
SalI G'TCGA_C
Cuts at: 1936 3239 4798 1936
Size: 1303 1559 4594
Fragments arranged by size:
4594 1559 1303
SapI GCTCTTCn'nnn_
Cuts at: 3714 3714
Size: 7456
SbfI CC_TGCA'GG
Cuts at: 4808 4808
Size: 7456
ScaI AGT'ACT
Cuts at: 5704 7416 5704
Size: 1712 5744
SexAI A'CCwGG_T
Cuts at: 2055 2055
Size: 7456
SfcI C'TryA_G
Cuts at: 826 1045 4804 5939 6620 6811 826
Size: 219 3759 1135 681 191 1471
Fragments arranged by size:
3759 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 3178 3178
Size: 7456
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7456
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7456
SmaI CCC'GGG
Cuts at: 1872 2162 4340 4783 1872
Size: 290 2178 443 4545
Fragments arranged by size:
4545 2178 443 290
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5560 6461 6723 820
Size: 197 926 3617 901 262 1553
Fragments arranged by size:
3617 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5247 493
Size: 4754 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7456
SphI G_CATG'C
Cuts at: 1132 4814 1132
Size: 3682 3774
SspI AAT'ATT
Cuts at: 5 52 4199 5380 5
Size: 47 4147 1181 2081
Fragments arranged by size:
4147 2081 1181 47
StuI AGG'CCT
Cuts at: 1517 3048 1517
Size: 1531 5925
StyI C'CwwG_G
Cuts at: 513 1065 2946 3366 4895 513
Size: 552 1881 420 1529 3074
Fragments arranged by size:
3074 1881 1529 552 420
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3229 3279 3587 4244 4398 5483 5500
Size: 1255 1649 50 308 657 154 1085 17
Cuts at: 5500 5653 5838 6283 325
Size: 153 185 445 1498
Fragments arranged by size:
1649 1498 1255 1085 657 445 308 185
154 153 50 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2904 5702 7414
Size: 274 80 33 51 1443 927 2798 1712
Cuts at: 7414 96
Size: 138
Fragments arranged by size:
2798 1712 1443 927 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2312 2344 2541 2573 3725 3757 4136 4168 7416
Size: 32 197 32 1152 32 379 32 3248
Cuts at: 7416 7448 2312
Size: 32 2320
Fragments arranged by size:
3248 2320 1152 379 197 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2057 3107 1316
Size: 741 1050 5665
Fragments arranged by size:
5665 1050 741
XbaI T'CTAG_A
Cuts at: 4792 4792
Size: 7456
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3793 3793
Size: 7456
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7456
XmaI C'CCGG_G
Cuts at: 1870 2160 4338 4781 1870
Size: 290 2178 443 4545
Fragments arranged by size:
4545 2178 443 290
XmnI GAAnn'nnTTC
Cuts at: 4298 5585 4298
Size: 1287 6169
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5264 276
Size: 53 83 186 936 3730 2468
Fragments arranged by size:
3730 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SfiI SrfI SwaI