(Circular) (Six-base) MAPSORT of: pFN21AB4596.seq Check: 7311 from: 1 to: 7456 pFN21AB4596 7456 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4448 4448 Size: 7456 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5266 278 Size: 53 83 186 936 3730 2468 Fragments arranged by size: 3730 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3240 4799 1937 Size: 1303 1559 4594 Fragments arranged by size: 4594 1559 1303 Acc65I G'GTAC_C Cuts at: 4777 4777 Size: 7456 AclI AA'CG_TT Cuts at: 1563 5583 5956 1563 Size: 4020 373 3063 Fragments arranged by size: 4020 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2066 2116 4352 5519 6903 7171 1632 Size: 434 50 2236 1167 1384 268 1917 Fragments arranged by size: 2236 1917 1384 1167 434 268 50 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7259 AflIII A'CryG_T Cuts at: 1203 4665 6355 7332 1203 Size: 3462 1690 977 1327 Fragments arranged by size: 3462 1690 1327 977 AgeI A'CCGG_T Cuts at: 6287 6287 Size: 7456 AhdI GACnn_n'nnGTC Cuts at: 6185 6185 Size: 7456 AleI CACnn'nnGTG Cuts at: 1166 2367 4501 4721 1166 Size: 1201 2134 220 3901 Fragments arranged by size: 3901 2134 1201 220 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3537 3901 1184 Size: 2353 364 4739 Fragments arranged by size: 4739 2353 364 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2904 2936 2904 Size: 32 7424 AlwNI CAG_nnn'CTG Cuts at: 2023 5199 6771 2023 Size: 3176 1572 2708 Fragments arranged by size: 3176 2708 1572 ApaLI G'TGCA_C Cuts at: 2360 5513 6669 2360 Size: 3153 1156 3147 Fragments arranged by size: 3153 3147 1156 ApoI r'AATT_y Cuts at: 1493 2277 3941 4765 4991 5143 7252 1493 Size: 784 1664 824 226 152 2109 1697 Fragments arranged by size: 2109 1697 1664 824 784 226 152 AseI AT'TA_AT Cuts at: 160 1032 6010 160 Size: 872 4978 1606 Fragments arranged by size: 4978 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7456 AvaI C'yCGr_G Cuts at: 1870 1943 2160 2994 4338 4781 1870 Size: 73 217 834 1344 443 4545 Fragments arranged by size: 4545 1344 834 443 217 73 BamHI G'GATC_C Cuts at: 2855 4786 5153 2855 Size: 1931 367 5158 Fragments arranged by size: 5158 1931 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2639 2702 3176 3307 3983 Size: 325 217 627 852 63 474 131 676 Cuts at: 3983 4777 6232 618 Size: 794 1455 1842 Fragments arranged by size: 1842 1455 852 794 676 627 474 325 217 131 63 BanII G_rGCy'C Cuts at: 1391 4014 4775 1391 Size: 2623 761 4072 Fragments arranged by size: 4072 2623 761 BbeI G_GCGC'C Cuts at: 3180 3180 Size: 7456 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2259 2823 4340 928 Size: 971 360 564 1517 4044 Fragments arranged by size: 4044 1517 971 564 360 BbvCI CC'TCA_GC Cuts at: 3500 3500 Size: 7456 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5647 5681 1903 Size: 34 3710 34 3678 Fragments arranged by size: 3710 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4463 5348 6558 4463 Size: 885 1210 5361 Fragments arranged by size: 5361 1210 885 BclI T'GATC_A Cuts at: 1548 2386 2508 2688 3730 3852 4236 1548 Size: 838 122 180 1042 122 384 4768 Fragments arranged by size: 4768 1042 838 384 180 122 122 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6067 136 Size: 107 122 71 631 605 4395 1525 Fragments arranged by size: 4395 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3678 4317 7451 3678 Size: 639 3134 3683 Fragments arranged by size: 3683 3134 639 BlpI GC'TnA_GC Cuts at: 3148 4873 3148 Size: 1725 5731 Bme1580I G_kGCm'C Cuts at: 1790 2364 2642 3212 3713 3986 5517 6673 1790 Size: 574 278 570 501 273 1531 1156 2573 Fragments arranged by size: 2573 1531 1156 574 570 501 278 273 BmgBI CAC'GTC Cuts at: 1318 3955 1318 Size: 2637 4819 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3182 3723 5187 Size: 416 53 83 399 24 1765 541 1464 Cuts at: 5187 6145 442 Size: 958 1753 Fragments arranged by size: 1765 1753 1464 958 541 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7456 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3479 4382 4400 4603 6116 1239 Size: 2240 903 18 203 1513 2579 Fragments arranged by size: 2579 2240 1513 903 203 18 Bpu10I CC'TnA_GC Cuts at: 3500 3500 Size: 7456 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5581 6446 6744 5581 Size: 865 298 6293 Fragments arranged by size: 6293 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3413 4346 6119 882 Size: 617 1914 933 1773 2219 Fragments arranged by size: 2219 1914 1773 933 617 BsaAI yAC'GTr Cuts at: 493 1204 3858 5247 493 Size: 711 2654 1389 2702 Fragments arranged by size: 2702 2654 1389 711 BsaBI GATnn'nnATC Cuts at: 2169 2771 4115 7450 2169 Size: 602 1344 3335 2175 Fragments arranged by size: 3335 2175 1344 602 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3177 5263 5645 6280 Size: 53 83 186 936 1644 2086 382 635 Cuts at: 6280 275 Size: 1451 Fragments arranged by size: 2086 1644 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2823 3552 5889 6287 6561 6708 7119 2823 Size: 729 2337 398 274 147 411 3160 Fragments arranged by size: 3160 2337 729 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2541 2571 2755 2785 2904 Size: 30 165 30 1138 30 184 30 119 Cuts at: 2904 2934 3725 3755 1178 Size: 30 791 30 4879 Fragments arranged by size: 4879 1138 791 184 165 119 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3323 3431 4022 4082 1186 Size: 2137 108 591 60 4560 Fragments arranged by size: 4560 2137 591 108 60 BseYI C'CCAG_C Cuts at: 3493 4211 6659 7155 3493 Size: 718 2448 496 3794 Fragments arranged by size: 3794 2448 718 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6883 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4722 4906 5667 6695 1540 Size: 275 186 2721 184 761 1028 2301 Fragments arranged by size: 2721 2301 1028 761 275 186 184 BsiHKAI G_wGCw'C Cuts at: 2364 5517 5602 6673 2364 Size: 3153 85 1071 3147 Fragments arranged by size: 3153 3147 1071 85 BsmI GAATG_Cn' Cuts at: 4967 5060 4967 Size: 93 7363 BsmBI CGTCTCn'nnnn_ Cuts at: 2748 2793 4319 2748 Size: 45 1526 5885 Fragments arranged by size: 5885 1526 45 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2364 2642 3212 3713 3986 4014 4775 Size: 399 574 278 570 501 273 28 761 Cuts at: 4775 5517 5602 6673 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 761 742 574 570 501 399 278 273 85 28 BspEI T'CCGG_A Cuts at: 2823 3552 7119 2823 Size: 729 3567 3160 Fragments arranged by size: 3567 3160 729 BspHI T'CATG_A Cuts at: 2121 2299 5345 7075 2121 Size: 178 3046 1730 2502 Fragments arranged by size: 3046 2502 1730 178 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4448 4811 844 Size: 3604 363 3489 Fragments arranged by size: 3604 3489 363 BsrBI CCG'CTC Cuts at: 1394 1823 2538 5343 1394 Size: 429 715 2805 3507 Fragments arranged by size: 3507 2805 715 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2244 4057 5951 6125 65 Size: 1183 996 1813 1894 174 1396 Fragments arranged by size: 1894 1813 1396 1183 996 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3405 4043 6100 6287 1700 Size: 110 1595 638 2057 187 2869 Fragments arranged by size: 2869 2057 1595 638 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7456 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7456 BssSI C'ACGA_G Cuts at: 5516 6528 5516 Size: 1012 6444 BstAPI GCAn_nnn'nTGC Cuts at: 794 4056 794 Size: 3262 4194 BstBI TT'CG_AA Cuts at: 2275 6260 2275 Size: 3985 3471 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7456 BstXI CCAn_nnnn'nTGG Cuts at: 3160 3160 Size: 7456 BstYI r'GATC_y Cuts at: 1972 2254 2855 3245 3598 3678 4317 4536 4786 Size: 282 601 390 353 80 639 219 250 Cuts at: 4786 5153 5538 5555 7007 7093 7105 7451 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 639 601 390 385 367 353 346 282 250 219 86 80 17 12 BtgI C'CryG_G Cuts at: 513 1065 3228 3366 3525 4722 513 Size: 552 2163 138 159 1197 3247 Fragments arranged by size: 3247 2163 1197 552 159 138 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4458 487 Size: 683 56 3232 3485 Fragments arranged by size: 3485 3232 683 56 BtsI GCAGTG_nn' Cuts at: 2374 4391 4972 5766 5786 2374 Size: 2017 581 794 20 4044 Fragments arranged by size: 4044 2017 794 581 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7456 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7421 DraI TTT'AAA Cuts at: 2530 4760 2530 Size: 2230 5226 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4560 6463 809 Size: 507 3244 1903 1802 Fragments arranged by size: 3244 1903 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2943 3393 4719 Size: 54 1425 50 275 18 1113 450 1326 Cuts at: 4719 4903 5792 7421 8 Size: 184 889 1629 43 Fragments arranged by size: 1629 1425 1326 1113 889 450 275 184 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4719 4903 1537 Size: 275 2907 184 4090 Fragments arranged by size: 4090 2907 275 184 EarI CTCTTCn'nnn_ Cuts at: 1353 3464 3714 4310 5386 1353 Size: 2111 250 596 1076 3423 Fragments arranged by size: 3423 2111 1076 596 250 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2986 6043 6417 6563 387 Size: 1064 138 1397 3057 374 146 1280 Fragments arranged by size: 3057 1397 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2116 3479 4352 4382 4400 4603 Size: 393 434 50 1363 873 30 18 203 Cuts at: 4603 5519 6116 6903 7171 1239 Size: 916 597 787 268 1524 Fragments arranged by size: 1524 1363 916 873 787 597 434 393 268 203 50 30 18 EcoNI CCTnn'n_nnAGG Cuts at: 3913 4682 3913 Size: 769 6687 EcoO109I rG'GnC_Cy Cuts at: 2953 3782 4134 2953 Size: 829 352 6275 Fragments arranged by size: 6275 829 352 EcoRI G'AATT_C Cuts at: 2277 4765 5143 2277 Size: 2488 378 4590 Fragments arranged by size: 4590 2488 378 EcoRV GAT'ATC Cuts at: 2119 4165 2119 Size: 2046 5410 FspI TGC'GCA Cuts at: 5962 5962 Size: 7456 HaeII r_GCGC'y Cuts at: 1678 3180 3304 6603 1678 Size: 1502 124 3299 2531 Fragments arranged by size: 3299 2531 1502 124 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2792 2824 2840 2872 3095 Size: 32 135 32 1220 32 16 32 223 Cuts at: 3095 3127 3412 3444 4302 4334 6097 6129 6171 Size: 32 285 32 858 32 1763 32 42 Cuts at: 6171 6203 1373 Size: 32 2626 Fragments arranged by size: 2626 1763 1220 858 285 223 135 42 32 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 3241 3970 4800 5046 669 Size: 988 281 1303 729 830 246 3079 Fragments arranged by size: 3079 1303 988 830 729 281 246 HindIII A'AGCT_T Cuts at: 748 4601 748 Size: 3853 3603 HpaI GTT'AAC Cuts at: 5046 5046 Size: 7456 KasI G'GCGC_C Cuts at: 3176 3176 Size: 7456 KpnI G_GTAC'C Cuts at: 4781 4781 Size: 7456 MfeI C'AATT_G Cuts at: 5055 5055 Size: 7456 MluI A'CGCG_T Cuts at: 7332 7332 Size: 7456 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2198 4006 6570 6754 1305 Size: 893 1808 2564 184 2007 Fragments arranged by size: 2564 2007 1808 893 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2945 10 Size: 54 1425 343 1113 4521 Fragments arranged by size: 4521 1425 1113 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2367 2645 3519 3989 4121 4501 4657 Size: 648 1201 278 874 470 132 380 156 Cuts at: 4657 4721 5414 5773 5932 518 Size: 64 693 359 159 2042 Fragments arranged by size: 2042 1201 874 693 648 470 380 359 278 159 156 132 64 MspA1I CmG'CkG Cuts at: 1606 2878 3351 4501 4695 4872 5549 6697 6942 Size: 1272 473 1150 194 177 677 1148 245 Cuts at: 6942 1606 Size: 2120 Fragments arranged by size: 2120 1272 1150 1148 677 473 245 194 177 NarI GG'CG_CC Cuts at: 3177 3177 Size: 7456 NcoI C'CATG_G Cuts at: 513 1065 3366 513 Size: 552 2301 4603 Fragments arranged by size: 4603 2301 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7456 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7456 NotI GC'GGCC_GC Cuts at: 4903 4903 Size: 7456 NspI r_CATG'y Cuts at: 1132 1229 2905 3454 4814 6359 1132 Size: 97 1676 549 1360 1545 2229 Fragments arranged by size: 2229 1676 1545 1360 549 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7456 PciI A'CATG_T Cuts at: 6355 6355 Size: 7456 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7456 PfoI T'CCnGG_A Cuts at: 4484 4484 Size: 7456 PmeI GTTT'AAAC Cuts at: 4760 4760 Size: 7456 PmlI CAC'GTG Cuts at: 3858 3858 Size: 7456 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2904 2936 5525 5557 7038 7070 2904 Size: 32 2589 32 1481 32 3290 Fragments arranged by size: 3290 2589 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7456 PsiI TTA'TAA Cuts at: 5026 5026 Size: 7456 PstI C_TGCA'G Cuts at: 830 4808 830 Size: 3978 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7456 PvuII CAG'CTG Cuts at: 2878 4501 2878 Size: 1623 5833 SalI G'TCGA_C Cuts at: 1936 3239 4798 1936 Size: 1303 1559 4594 Fragments arranged by size: 4594 1559 1303 SapI GCTCTTCn'nnn_ Cuts at: 3714 3714 Size: 7456 SbfI CC_TGCA'GG Cuts at: 4808 4808 Size: 7456 ScaI AGT'ACT Cuts at: 5704 7416 5704 Size: 1712 5744 SexAI A'CCwGG_T Cuts at: 2055 2055 Size: 7456 SfcI C'TryA_G Cuts at: 826 1045 4804 5939 6620 6811 826 Size: 219 3759 1135 681 191 1471 Fragments arranged by size: 3759 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 3178 3178 Size: 7456 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7456 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7456 SmaI CCC'GGG Cuts at: 1872 2162 4340 4783 1872 Size: 290 2178 443 4545 Fragments arranged by size: 4545 2178 443 290 SmlI C'TyrA_G Cuts at: 820 1017 1943 5560 6461 6723 820 Size: 197 926 3617 901 262 1553 Fragments arranged by size: 3617 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5247 493 Size: 4754 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7456 SphI G_CATG'C Cuts at: 1132 4814 1132 Size: 3682 3774 SspI AAT'ATT Cuts at: 5 52 4199 5380 5 Size: 47 4147 1181 2081 Fragments arranged by size: 4147 2081 1181 47 StuI AGG'CCT Cuts at: 1517 3048 1517 Size: 1531 5925 StyI C'CwwG_G Cuts at: 513 1065 2946 3366 4895 513 Size: 552 1881 420 1529 3074 Fragments arranged by size: 3074 1881 1529 552 420 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3229 3279 3587 4244 4398 5483 5500 Size: 1255 1649 50 308 657 154 1085 17 Cuts at: 5500 5653 5838 6283 325 Size: 153 185 445 1498 Fragments arranged by size: 1649 1498 1255 1085 657 445 308 185 154 153 50 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2904 5702 7414 Size: 274 80 33 51 1443 927 2798 1712 Cuts at: 7414 96 Size: 138 Fragments arranged by size: 2798 1712 1443 927 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2312 2344 2541 2573 3725 3757 4136 4168 7416 Size: 32 197 32 1152 32 379 32 3248 Cuts at: 7416 7448 2312 Size: 32 2320 Fragments arranged by size: 3248 2320 1152 379 197 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2057 3107 1316 Size: 741 1050 5665 Fragments arranged by size: 5665 1050 741 XbaI T'CTAG_A Cuts at: 4792 4792 Size: 7456 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3793 3793 Size: 7456 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7456 XmaI C'CCGG_G Cuts at: 1870 2160 4338 4781 1870 Size: 290 2178 443 4545 Fragments arranged by size: 4545 2178 443 290 XmnI GAAnn'nnTTC Cuts at: 4298 5585 4298 Size: 1287 6169 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5264 276 Size: 53 83 186 936 3730 2468 Fragments arranged by size: 3730 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SrfI SwaI