Restriction map for
pFN21AB4590
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# NO CUT ENZYMES ( 131 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BbeI BbrPI BcgI
BciVI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstF5I BstPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraII DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoT22I FokI
FseI FspI HgaI Hin1I HindIII
HpaI KasI KpnI KspI MaeII
MluI MroI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI SspBI SspI StuI
SwaI Tth111I XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 173 GTCTAC GT^[AC][GT]AC
AflII 244 CTTAAG C^TTAAG
AflIII 1958 ACATGT A^C[GA][CT]GT
AlwNI 1912 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 346 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1658 GAGCTC G[AT]GC[AT]^C
BalI 275 TGGCCA TGG^CCA
BamHI 1009 GGATCC G^GATCC
BanI 679 GGCACC G^G[CT][GA]CC
BanII 1658 GAGCTC G[GA]GC[CT]^C
BbvCI 2002 GCTGAGG CCTCAGC(-5/-2)
BcnI 1434 CCGGG CC^[GC]GG
BfrI 244 CTTAAG C^TTAAG
BmyI 1658 GAGCTC G[AGT]GC[ACT]^C
BsaBI 1897 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 1658 GAGCTC G[AT]GC[AT]^C
Bsp1286I 1658 GAGCTC G[AGT]GC[ACT]^C
BspHI 1905 TCATGA T^CATGA
BspLU11I 1958 ACATGT A^CATGT
BsrFI 1380 ACCGGC [GA]^CCGG[CT]
Cfr10I 1380 ACCGGC [GA]^CCGG[CT]
EcoRI 1078 GAATTC G^AATTC
EcoRV 919 GATATC GAT^ATC
FauI 1574 CCCGC CCCGC(4/6)
HaeII 1705 GGCGCT [GA]GCGC^[CT]
HincII 1216 GTTGAC GT[CT]^[GA]AC
HindII 1216 GTTGAC GT[CT]^[GA]AC
MamI 1897 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 907 CAATTG C^AATTG
MluNI 275 TGGCCA TGG^CCA
MscI 275 TGGCCA TGG^CCA
MunI 907 CAATTG C^AATTG
NciI 1434 CCGGG CC^[GC]GG
PflMI 1911 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1678 GACCAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1905 TCATGA T^CATGA
SacI 1658 GAGCTC GAGCT^C
SbfI 1020 CCTGCAGG CCTGCA^GG
SmlI 244 CTTAAG C^T[CT][GA]AG
Sse8387I 1020 CCTGCAGG CCTGCA^GG
TaqI 1067 TCGA T^CGA
TthHB8I 1067 TCGA T^CGA
Van91I 1911 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1650 TCTAGA T^CTAGA
XmnI 346 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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BclI 1880 TGATCA T^GATCA
1902 TGATCA
BsaI 577 GAGACC GGTCTC(1/5)
1638 GGTCTC
BsgI 1132 CTGCAC GTGCAG(16/14)
589 GTGCAG
BsiYI 1524 CCTGACCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1911 CCAGGCACTGG
BslI 1524 CCTGACCTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1911 CCAGGCACTGG
BsmFI 717 GGGAC GGGAC(10/14)
1769 GGGAC
BsrDI 960 CATTGC GCAATG(2/0)
1749 CATTGC
BstYI 819 AGATCC [GA]^GATC[CT]
1009 GGATCC
CfoI 1620 GCGC GCG^C
1706 GCGC
DrdI 1527 GACCTTGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1957 GACATGTCGGTC
FbaI 1880 TGATCA T^GATCA
1902 TGATCA
HhaI 1620 GCGC GCG^C
1706 GCGC
HinP1I 1620 GCGC G^CGC
1706 GCGC
MflI 819 AGATCC [GA]^GATC[CT]
1009 GGATCC
MslI 1749 CATTGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1968 CACAAGAGTG
MspA1I 155 CAGCTG C[AC]G^C[GT]G
690 CAGCTG
NcoI 238 CCATGG C^CATGG
859 CCATGG
NspI 708 ACATGC [GA]CATG^[CT]
1958 ACATGT
PstI 152 CTGCAG CTGCA^G
1021 CTGCAG
PvuII 155 CAGCTG CAG^CTG
690 CAGCTG
XhoII 819 AGATCC [GA]^GATC[CT]
1009 GGATCC
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbsI BbvI BfaI
BpmI BpuAI BsaJI BseRI BsmAI
BsrI BssKI BstNI Cac8I Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII EcoT14I Fnu4HI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI