(Circular) (Six-base) MAPSORT of: pFN21AB4590.seq Check: 2967 from: 1 to: 6724 pFN21AB4590 6724 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4534 278 Size: 53 83 186 936 2998 2468 Fragments arranged by size: 2998 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2179 4067 1937 Size: 242 1888 4594 Fragments arranged by size: 4594 1888 242 Acc65I G'GTAC_C Cuts at: 4045 4045 Size: 6724 AclI AA'CG_TT Cuts at: 1563 4851 5224 1563 Size: 3288 373 3063 Fragments arranged by size: 3288 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2034 2052 2412 2933 2940 4787 6171 6439 Size: 402 18 360 521 7 1847 1384 268 Cuts at: 6439 1632 Size: 1917 Fragments arranged by size: 1917 1847 1384 521 402 360 268 18 7 AflII C'TTAA_G Cuts at: 820 1017 2249 820 Size: 197 1232 5295 Fragments arranged by size: 5295 1232 197 AflIII A'CryG_T Cuts at: 1203 3963 5623 6600 1203 Size: 2760 1660 977 1327 Fragments arranged by size: 2760 1660 1327 977 AgeI A'CCGG_T Cuts at: 5555 5555 Size: 6724 AhdI GACnn_n'nnGTC Cuts at: 5453 5453 Size: 6724 AleI CACnn'nnGTG Cuts at: 1166 3977 1166 Size: 2811 3913 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2982 1184 Size: 1798 4926 AlwNI CAG_nnn'CTG Cuts at: 3922 4467 6039 3922 Size: 545 1572 4607 Fragments arranged by size: 4607 1572 545 ApaLI G'TGCA_C Cuts at: 4781 5937 4781 Size: 1156 5568 ApoI r'AATT_y Cuts at: 1493 3083 3345 3545 3852 4033 4259 4411 6520 Size: 1590 262 200 307 181 226 152 2109 Cuts at: 6520 1493 Size: 1697 Fragments arranged by size: 2109 1697 1590 307 262 226 200 181 152 AseI AT'TA_AT Cuts at: 160 1032 5278 160 Size: 872 4246 1606 Fragments arranged by size: 4246 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6724 AvaI C'yCGr_G Cuts at: 1870 1943 4049 1870 Size: 73 2106 4545 Fragments arranged by size: 4545 2106 73 BamHI G'GATC_C Cuts at: 3014 4054 4421 3014 Size: 1040 367 5317 Fragments arranged by size: 5317 1040 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2684 4045 5500 618 Size: 325 217 627 897 1361 1455 1842 Fragments arranged by size: 1842 1455 1361 897 627 325 217 BanII G_rGCy'C Cuts at: 1391 3667 4043 1391 Size: 2276 376 4072 Fragments arranged by size: 4072 2276 376 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2109 2556 2651 928 Size: 971 210 447 95 5001 Fragments arranged by size: 5001 971 447 210 95 BbvCI CC'TCA_GC Cuts at: 4008 4008 Size: 6724 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4915 4949 1903 Size: 34 2978 34 3678 Fragments arranged by size: 3678 2978 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4616 5826 4616 Size: 1210 5514 BclI T'GATC_A Cuts at: 1548 3885 3907 1548 Size: 2337 22 4365 Fragments arranged by size: 4365 2337 22 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5335 136 Size: 107 122 71 631 605 3663 1525 Fragments arranged by size: 3663 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6719 6719 Size: 6724 BlpI GC'TnA_GC Cuts at: 4141 4141 Size: 6724 Bme1580I G_kGCm'C Cuts at: 1790 4785 5941 1790 Size: 2995 1156 2573 Fragments arranged by size: 2995 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6724 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3175 4455 5413 Size: 416 53 83 399 24 1758 1280 958 Cuts at: 5413 442 Size: 1753 Fragments arranged by size: 1758 1753 1280 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6724 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2726 3486 3631 5384 1239 Size: 1487 760 145 1753 2579 Fragments arranged by size: 2579 1753 1487 760 145 Bpu10I CC'TnA_GC Cuts at: 2379 2805 3927 4008 2379 Size: 426 1122 81 5095 Fragments arranged by size: 5095 1122 426 81 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4849 5714 6012 4849 Size: 865 298 5561 Fragments arranged by size: 5561 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2576 3649 5387 882 Size: 617 1077 1073 1738 2219 Fragments arranged by size: 2219 1738 1077 1073 617 BsaAI yAC'GTr Cuts at: 493 1204 4515 493 Size: 711 3311 2702 Fragments arranged by size: 3311 2702 711 BsaBI GATnn'nnATC Cuts at: 3906 6718 3906 Size: 2812 3912 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4531 4913 5548 275 Size: 53 83 186 936 2998 382 635 1451 Fragments arranged by size: 2998 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5157 5555 5829 5976 6387 5157 Size: 398 274 147 411 5494 Fragments arranged by size: 5494 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6499 Fragments arranged by size: 6499 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2144 2307 2580 1186 Size: 958 163 273 5330 Fragments arranged by size: 5330 958 273 163 BseYI C'CCAG_C Cuts at: 2145 3265 3716 5927 6423 2145 Size: 1120 451 2211 496 2446 Fragments arranged by size: 2446 2211 1120 496 451 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2615 3122 1163 Size: 573 879 507 4765 Fragments arranged by size: 4765 879 573 507 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4174 4935 5963 1540 Size: 275 186 2173 761 1028 2301 Fragments arranged by size: 2301 2173 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3667 4785 4870 5941 3667 Size: 1118 85 1071 4450 Fragments arranged by size: 4450 1118 1071 85 BsmI GAATG_Cn' Cuts at: 4235 4328 4235 Size: 93 6631 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3667 4043 4785 4870 5941 1391 Size: 399 1877 376 742 85 1071 2174 Fragments arranged by size: 2174 1877 1071 742 399 376 85 BspEI T'CCGG_A Cuts at: 6387 6387 Size: 6724 BspHI T'CATG_A Cuts at: 3910 4613 6343 3910 Size: 703 1730 4291 Fragments arranged by size: 4291 1730 703 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4079 844 Size: 3235 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4611 1394 Size: 429 2788 3507 Fragments arranged by size: 3507 2788 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2964 3753 5219 5393 65 Size: 1183 1716 789 1466 174 1396 Fragments arranged by size: 1716 1466 1396 1183 789 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3385 5368 5555 1700 Size: 110 1575 1983 187 2869 Fragments arranged by size: 2869 1983 1575 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6724 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6724 BssSI C'ACGA_G Cuts at: 4784 5796 4784 Size: 1012 5712 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6724 BstBI TT'CG_AA Cuts at: 5528 5528 Size: 6724 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6724 BstYI r'GATC_y Cuts at: 1972 2824 3014 4054 4421 4806 4823 6275 6361 Size: 852 190 1040 367 385 17 1452 86 Cuts at: 6361 6373 6719 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1040 852 385 367 346 190 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2243 2864 3759 513 Size: 552 1178 621 895 3478 Fragments arranged by size: 3478 1178 895 621 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5985 Fragments arranged by size: 5985 683 56 BtsI GCAGTG_nn' Cuts at: 4240 5034 5054 4240 Size: 794 20 5910 Fragments arranged by size: 5910 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6724 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3963 3998 529 Size: 35 3399 35 3255 Fragments arranged by size: 3399 3255 35 35 DraI TTT'AAA Cuts at: 4028 4028 Size: 6724 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3538 3968 5731 809 Size: 507 2222 430 1763 1802 Fragments arranged by size: 2222 1802 1763 507 430 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2280 2974 3329 Size: 54 1425 50 275 18 450 694 355 Cuts at: 3329 3559 4171 5060 6689 8 Size: 230 612 889 1629 43 Fragments arranged by size: 1629 1425 889 694 612 450 355 275 230 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4171 1537 Size: 275 2359 4090 Fragments arranged by size: 4090 2359 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2186 2520 3114 4654 1353 Size: 833 334 594 1540 3423 Fragments arranged by size: 3423 1540 833 594 334 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5311 5685 5831 387 Size: 1064 138 3722 374 146 1280 Fragments arranged by size: 3722 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3665 3665 Size: 6724 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2034 2052 2412 2726 2933 2940 3486 Size: 393 402 18 360 314 207 7 546 Cuts at: 3486 3631 4787 5384 6171 6439 1239 Size: 145 1156 597 787 268 1524 Fragments arranged by size: 1524 1156 787 597 546 402 393 360 314 268 207 145 18 7 EcoRI G'AATT_C Cuts at: 3083 4033 4411 3083 Size: 950 378 5396 Fragments arranged by size: 5396 950 378 EcoRV GAT'ATC Cuts at: 2926 2926 Size: 6724 FspI TGC'GCA Cuts at: 5230 5230 Size: 6724 HaeII r_GCGC'y Cuts at: 1678 3714 5871 1678 Size: 2036 2157 2531 Fragments arranged by size: 2531 2157 2036 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2609 2641 2954 2986 2989 Size: 32 135 32 1037 32 313 32 3 Cuts at: 2989 3021 3297 3329 5365 5397 5439 5471 1373 Size: 32 276 32 2036 32 42 32 2626 Fragments arranged by size: 2626 2036 1037 313 276 135 42 32 32 32 32 32 32 32 32 3 HincII GTy'rAC Cuts at: 669 1657 1938 3223 4068 4314 669 Size: 988 281 1285 845 246 3079 Fragments arranged by size: 3079 1285 988 845 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6724 HpaI GTT'AAC Cuts at: 4314 4314 Size: 6724 KpnI G_GTAC'C Cuts at: 4049 4049 Size: 6724 MfeI C'AATT_G Cuts at: 2912 4323 2912 Size: 1411 5313 MluI A'CGCG_T Cuts at: 6600 6600 Size: 6724 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5838 6022 1305 Size: 4533 184 2007 Fragments arranged by size: 4533 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2282 10 Size: 54 1425 343 450 4452 Fragments arranged by size: 4452 1425 450 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3758 3977 4682 5041 5200 518 Size: 648 2592 219 705 359 159 2042 Fragments arranged by size: 2592 2042 705 648 359 219 159 MspA1I CmG'CkG Cuts at: 1606 2162 2697 4140 4817 5965 6210 1606 Size: 556 535 1443 677 1148 245 2120 Fragments arranged by size: 2120 1443 1148 677 556 535 245 NcoI C'CATG_G Cuts at: 513 1065 2243 2864 513 Size: 552 1178 621 4373 Fragments arranged by size: 4373 1178 621 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6724 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6724 NotI GC'GGCC_GC Cuts at: 4171 4171 Size: 6724 NspI r_CATG'y Cuts at: 1132 1229 2717 3967 4082 5627 1132 Size: 97 1488 1250 115 1545 2229 Fragments arranged by size: 2229 1545 1488 1250 115 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6724 PciI A'CATG_T Cuts at: 3963 5623 3963 Size: 1660 5064 PflMI CCAn_nnn'nTGG Cuts at: 1108 3922 1108 Size: 2814 3910 PfoI T'CCnGG_A Cuts at: 3647 3647 Size: 6724 PmeI GTTT'AAAC Cuts at: 4028 4028 Size: 6724 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4793 4825 6306 6338 4793 Size: 32 1481 32 5179 Fragments arranged by size: 5179 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3687 1613 Size: 2074 4650 PsiI TTA'TAA Cuts at: 4294 4294 Size: 6724 PstI C_TGCA'G Cuts at: 830 2161 3030 4076 830 Size: 1331 869 1046 3478 Fragments arranged by size: 3478 1331 1046 869 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6724 PvuII CAG'CTG Cuts at: 2162 2697 2162 Size: 535 6189 SacI G_AGCT'C Cuts at: 3667 3667 Size: 6724 SalI G'TCGA_C Cuts at: 1936 4066 1936 Size: 2130 4594 SbfI CC_TGCA'GG Cuts at: 3030 4076 3030 Size: 1046 5678 ScaI AGT'ACT Cuts at: 4972 6684 4972 Size: 1712 5012 SfcI C'TryA_G Cuts at: 826 1045 2157 3026 3487 3553 4072 5207 5888 Size: 219 1112 869 461 66 519 1135 681 Cuts at: 5888 6079 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1112 869 681 519 461 219 191 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6724 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6724 SmaI CCC'GGG Cuts at: 1872 4051 1872 Size: 2179 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2249 4828 5729 5991 820 Size: 197 926 306 2579 901 262 1553 Fragments arranged by size: 2579 1553 926 901 306 262 197 SnaBI TAC'GTA Cuts at: 493 4515 493 Size: 4022 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6724 SphI G_CATG'C Cuts at: 1132 4082 1132 Size: 2950 3774 SspI AAT'ATT Cuts at: 5 52 4648 5 Size: 47 4596 2081 Fragments arranged by size: 4596 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6724 StyI C'CwwG_G Cuts at: 513 1065 2073 2243 2586 2718 2864 3237 3534 Size: 552 1008 170 343 132 146 373 297 Cuts at: 3534 4163 513 Size: 629 3074 Fragments arranged by size: 3074 1008 629 552 373 343 297 170 146 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3581 3958 4751 4768 4921 5106 5551 Size: 1255 2001 377 793 17 153 185 445 Cuts at: 5551 325 Size: 1498 Fragments arranged by size: 2001 1498 1255 793 445 377 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4970 6682 96 Size: 274 80 33 51 1443 2993 1712 138 Fragments arranged by size: 2993 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6684 6716 6684 Size: 32 6692 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6724 XbaI T'CTAG_A Cuts at: 3655 4060 3655 Size: 405 6319 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6724 XmaI C'CCGG_G Cuts at: 1870 4049 1870 Size: 2179 4545 XmnI GAAnn'nnTTC Cuts at: 2355 4853 2355 Size: 2498 4226 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4532 276 Size: 53 83 186 936 2998 2468 Fragments arranged by size: 2998 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI