(Circular) (Six-base) MAPSORT of: pFN21AB4573.seq Check: 235 from: 1 to: 6649 pFN21AB4573 6649 bp With 182 enzymes: SgfI * October 20, 2009 10:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3309 3309 Size: 6649 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4459 278 Size: 53 83 186 936 2923 2468 Fragments arranged by size: 2923 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2774 3144 3597 3992 1937 Size: 837 370 453 395 4594 Fragments arranged by size: 4594 837 453 395 370 Acc65I G'GTAC_C Cuts at: 3970 3970 Size: 6649 AclI AA'CG_TT Cuts at: 1563 4776 5149 1563 Size: 3213 373 3063 Fragments arranged by size: 3213 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2465 2766 2859 3157 4712 6096 6364 1632 Size: 833 301 93 298 1555 1384 268 1917 Fragments arranged by size: 1917 1555 1384 833 301 298 268 93 AfeI AGC'GCT Cuts at: 3862 3862 Size: 6649 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6452 AflIII A'CryG_T Cuts at: 1203 5548 6525 1203 Size: 4345 977 1327 Fragments arranged by size: 4345 1327 977 AgeI A'CCGG_T Cuts at: 5480 5480 Size: 6649 AhdI GACnn_n'nnGTC Cuts at: 2646 5378 2646 Size: 2732 3917 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6649 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2828 1184 Size: 1644 5005 AlwNI CAG_nnn'CTG Cuts at: 2969 3811 4392 5964 2969 Size: 842 581 1572 3654 Fragments arranged by size: 3654 1572 842 581 ApaLI G'TGCA_C Cuts at: 4706 5862 4706 Size: 1156 5493 ApoI r'AATT_y Cuts at: 1493 2474 2516 2596 3958 4184 4336 6445 1493 Size: 981 42 80 1362 226 152 2109 1697 Fragments arranged by size: 2109 1697 1362 981 226 152 80 42 AseI AT'TA_AT Cuts at: 160 1032 5203 160 Size: 872 4171 1606 Fragments arranged by size: 4171 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6649 AvaI C'yCGr_G Cuts at: 1870 1943 3931 3974 1870 Size: 73 1988 43 4545 Fragments arranged by size: 4545 1988 73 43 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2526 2559 2526 Size: 33 6616 BamHI G'GATC_C Cuts at: 3979 4346 3979 Size: 367 6282 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2959 3970 5425 618 Size: 325 217 627 1172 1011 1455 1842 Fragments arranged by size: 1842 1455 1172 1011 627 325 217 BanII G_rGCy'C Cuts at: 1391 2909 3968 1391 Size: 1518 1059 4072 Fragments arranged by size: 4072 1518 1059 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2036 2441 3807 3937 928 Size: 971 137 405 1366 130 3640 Fragments arranged by size: 3640 1366 971 405 137 130 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4840 4874 1903 Size: 34 2903 34 3678 Fragments arranged by size: 3678 2903 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4541 5751 4541 Size: 1210 5439 BclI T'GATC_A Cuts at: 1548 1548 Size: 6649 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2913 5260 136 Size: 107 122 71 631 605 1241 2347 1525 Fragments arranged by size: 2347 1525 1241 631 605 122 107 71 BglII A'GATC_T Cuts at: 3709 6644 3709 Size: 2935 3714 BlpI GC'TnA_GC Cuts at: 4066 4066 Size: 6649 Bme1580I G_kGCm'C Cuts at: 1790 2962 4710 5866 1790 Size: 1172 1748 1156 2573 Fragments arranged by size: 2573 1748 1172 1156 BmgBI CAC'GTC Cuts at: 1318 3927 1318 Size: 2609 4040 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3262 4380 5338 Size: 416 53 83 399 24 1845 1118 958 Cuts at: 5338 442 Size: 1753 Fragments arranged by size: 1845 1753 1118 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6649 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3516 3516 Size: 6649 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2062 2584 2716 3511 5309 1239 Size: 823 522 132 795 1798 2579 Fragments arranged by size: 2579 1798 823 795 522 132 Bpu10I CC'TnA_GC Cuts at: 3363 3363 Size: 6649 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2246 4774 5639 5937 2246 Size: 2528 865 298 2958 Fragments arranged by size: 2958 2528 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3308 5312 882 Size: 617 1809 2004 2219 Fragments arranged by size: 2219 2004 1809 617 BsaAI yAC'GTr Cuts at: 493 1204 4440 493 Size: 711 3236 2702 Fragments arranged by size: 3236 2702 711 BsaBI GATnn'nnATC Cuts at: 6643 6643 Size: 6649 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4456 4838 5473 275 Size: 53 83 186 936 2923 382 635 1451 Fragments arranged by size: 2923 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3855 5082 5480 5754 5901 6312 3855 Size: 1227 398 274 147 411 4192 Fragments arranged by size: 4192 1227 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3305 3335 1178 Size: 30 165 30 1902 30 4492 Fragments arranged by size: 4492 1902 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6649 BseYI C'CCAG_C Cuts at: 2623 5852 6348 2623 Size: 3229 496 2924 Fragments arranged by size: 3229 2924 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2313 1163 Size: 573 577 5499 Fragments arranged by size: 5499 577 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4099 4860 5888 1540 Size: 275 186 2098 761 1028 2301 Fragments arranged by size: 2301 2098 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2013 2529 4710 4795 5866 2013 Size: 516 2181 85 1071 2796 Fragments arranged by size: 2796 2181 1071 516 85 BsmI GAATG_Cn' Cuts at: 3564 4160 4253 3564 Size: 596 93 5960 Fragments arranged by size: 5960 596 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2529 2909 2962 3968 4710 4795 Size: 399 223 516 380 53 1006 742 85 Cuts at: 4795 5866 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1006 742 516 399 380 223 85 53 BspEI T'CCGG_A Cuts at: 6312 6312 Size: 6649 BspHI T'CATG_A Cuts at: 2521 4538 6268 2521 Size: 2017 1730 2902 Fragments arranged by size: 2902 2017 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3309 3496 4004 844 Size: 2465 187 508 3489 Fragments arranged by size: 3489 2465 508 187 BsrBI CCG'CTC Cuts at: 1394 1823 2956 4536 1394 Size: 429 1133 1580 3507 Fragments arranged by size: 3507 1580 1133 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2047 3345 3750 5144 5318 65 Size: 1183 799 1298 405 1394 174 1396 Fragments arranged by size: 1396 1394 1298 1183 799 405 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5293 5480 1700 Size: 110 3483 187 2869 Fragments arranged by size: 3483 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6649 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6649 BssSI C'ACGA_G Cuts at: 3000 4709 5721 3000 Size: 1709 1012 3928 Fragments arranged by size: 3928 1709 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6649 BstBI TT'CG_AA Cuts at: 2848 5453 2848 Size: 2605 4044 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6649 BstXI CCAn_nnnn'nTGG Cuts at: 2916 2916 Size: 6649 BstYI r'GATC_y Cuts at: 1972 2152 3129 3709 3807 3979 4346 4731 4748 Size: 180 977 580 98 172 367 385 17 Cuts at: 4748 6200 6286 6298 6644 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 977 580 385 367 346 180 172 98 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5158 Fragments arranged by size: 5158 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5910 Fragments arranged by size: 5910 683 56 BtsI GCAGTG_nn' Cuts at: 2301 4165 4959 4979 2301 Size: 1864 794 20 3971 Fragments arranged by size: 3971 1864 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6649 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3049 3084 529 Size: 35 2485 35 4094 Fragments arranged by size: 4094 2485 35 35 DraI TTT'AAA Cuts at: 3953 3953 Size: 6649 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5656 809 Size: 507 4340 1802 Fragments arranged by size: 4340 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4096 4985 6614 Size: 54 1425 50 275 18 2266 889 1629 Cuts at: 6614 8 Size: 43 Fragments arranged by size: 2266 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4096 1537 Size: 275 2284 4090 Fragments arranged by size: 4090 2284 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3743 3780 4579 1353 Size: 2390 37 799 3423 Fragments arranged by size: 3423 2390 799 37 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5236 5610 5756 387 Size: 1064 138 3647 374 146 1280 Fragments arranged by size: 3647 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2062 2465 2584 2716 2766 2859 3157 Size: 393 430 403 119 132 50 93 298 Cuts at: 3157 3511 4712 5309 6096 6364 1239 Size: 354 1201 597 787 268 1524 Fragments arranged by size: 1524 1201 787 597 430 403 393 354 298 268 132 119 93 50 EcoO109I rG'GnC_Cy Cuts at: 2272 2973 2272 Size: 701 5948 EcoRI G'AATT_C Cuts at: 3958 4336 3958 Size: 378 6271 EcoRV GAT'ATC Cuts at: 3904 3904 Size: 6649 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2470 2470 Size: 6649 FspI TGC'GCA Cuts at: 3197 5155 3197 Size: 1958 4691 HaeII r_GCGC'y Cuts at: 1678 2125 3864 5796 1678 Size: 447 1739 1932 2531 Fragments arranged by size: 2531 1932 1739 447 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 2632 2664 3398 Size: 32 135 32 773 32 255 32 734 Cuts at: 3398 3430 3584 3616 3657 3689 5290 5322 5364 Size: 32 154 32 41 32 1601 32 42 Cuts at: 5364 5396 1373 Size: 32 2626 Fragments arranged by size: 2626 1601 773 734 255 154 135 42 41 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2775 3145 3993 4239 669 Size: 988 281 837 370 848 246 3079 Fragments arranged by size: 3079 988 848 837 370 281 246 HindIII A'AGCT_T Cuts at: 748 2447 748 Size: 1699 4950 HpaI GTT'AAC Cuts at: 4239 4239 Size: 6649 KpnI G_GTAC'C Cuts at: 3974 3974 Size: 6649 MfeI C'AATT_G Cuts at: 3258 4248 3258 Size: 990 5659 MluI A'CGCG_T Cuts at: 6525 6525 Size: 6649 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3441 3570 3856 5763 5947 1305 Size: 2136 129 286 1907 184 2007 Fragments arranged by size: 2136 2007 1907 286 184 129 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4827 Fragments arranged by size: 4827 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3114 3303 3680 4607 4966 5125 518 Size: 648 1948 189 377 927 359 159 2042 Fragments arranged by size: 2042 1948 927 648 377 359 189 159 MspA1I CmG'CkG Cuts at: 1606 2082 4065 4742 5890 6135 1606 Size: 476 1983 677 1148 245 2120 Fragments arranged by size: 2120 1983 1148 677 476 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5158 Fragments arranged by size: 5158 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6649 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6649 NotI GC'GGCC_GC Cuts at: 4096 4096 Size: 6649 NspI r_CATG'y Cuts at: 1132 1229 3212 3308 3386 4007 5552 1132 Size: 97 1983 96 78 621 1545 2229 Fragments arranged by size: 2229 1983 1545 621 97 96 78 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6649 PciI A'CATG_T Cuts at: 5548 5548 Size: 6649 PflMI CCAn_nnn'nTGG Cuts at: 1108 2969 1108 Size: 1861 4788 PfoI T'CCnGG_A Cuts at: 2394 2669 3309 2394 Size: 275 640 5734 Fragments arranged by size: 5734 640 275 PmeI GTTT'AAAC Cuts at: 3953 3953 Size: 6649 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4718 4750 6231 6263 4718 Size: 32 1481 32 5104 Fragments arranged by size: 5104 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2973 2973 Size: 6649 PshAI GACnn'nnGTC Cuts at: 1613 2466 1613 Size: 853 5796 PsiI TTA'TAA Cuts at: 3096 4219 3096 Size: 1123 5526 PstI C_TGCA'G Cuts at: 830 4001 830 Size: 3171 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6649 PvuII CAG'CTG Cuts at: 2082 2082 Size: 6649 SalI G'TCGA_C Cuts at: 1936 2773 3143 3991 1936 Size: 837 370 848 4594 Fragments arranged by size: 4594 848 837 370 SanDI GG'GwC_CC Cuts at: 2973 2973 Size: 6649 SbfI CC_TGCA'GG Cuts at: 4001 4001 Size: 6649 ScaI AGT'ACT Cuts at: 4897 6609 4897 Size: 1712 4937 SfcI C'TryA_G Cuts at: 826 1045 2530 3997 5132 5813 6004 826 Size: 219 1485 1467 1135 681 191 1471 Fragments arranged by size: 1485 1471 1467 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6649 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6649 SmaI CCC'GGG Cuts at: 1872 3976 1872 Size: 2104 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2261 4753 5654 5916 820 Size: 197 926 318 2492 901 262 1553 Fragments arranged by size: 2492 1553 926 901 318 262 197 SnaBI TAC'GTA Cuts at: 493 4440 493 Size: 3947 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6649 SphI G_CATG'C Cuts at: 1132 3386 4007 1132 Size: 2254 621 3774 Fragments arranged by size: 3774 2254 621 SspI AAT'ATT Cuts at: 5 52 4573 5 Size: 47 4521 2081 Fragments arranged by size: 4521 2081 47 StuI AGG'CCT Cuts at: 1517 2998 3170 3793 1517 Size: 1481 172 623 4373 Fragments arranged by size: 4373 1481 623 172 StyI C'CwwG_G Cuts at: 513 1065 2004 4088 513 Size: 552 939 2084 3074 Fragments arranged by size: 3074 2084 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4676 4693 4846 5031 5476 325 Size: 1255 3096 17 153 185 445 1498 Fragments arranged by size: 3096 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4895 6607 96 Size: 274 80 33 51 1443 2918 1712 138 Fragments arranged by size: 2918 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2719 2751 2775 2807 6609 6641 2719 Size: 32 24 32 3802 32 2727 Fragments arranged by size: 3802 2727 32 32 32 24 Tth111I GACn'n_nGTC Cuts at: 1316 3925 1316 Size: 2609 4040 XbaI T'CTAG_A Cuts at: 3985 3985 Size: 6649 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2815 2815 Size: 6649 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6649 XmaI C'CCGG_G Cuts at: 1870 3974 1870 Size: 2104 4545 XmnI GAAnn'nnTTC Cuts at: 2379 2438 4778 2379 Size: 59 2340 4250 Fragments arranged by size: 4250 2340 59 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4457 276 Size: 53 83 186 936 2923 2468 Fragments arranged by size: 2923 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI