Restriction map for
pFN21AB4524
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# NO CUT ENZYMES ( 87 enzymes) #
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AatII Acc65I AccI AcyI AflII
AflIII AgeI Alw44I Aor51HI ApaLI
AscI Asp718I AviII AvrII BalI
BbeI BbrPI BciVI BfrI BglI
BlnI BsaBI BsaHI BsiWI BsmBI
BspDI BspLU11I BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
CfoI ClaI CpoI DraI DraIII
DrdI Eco47III EcoO65I EcoRV FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I KasI KpnI MamI MfeI
MluI MluNI MscI MunI NarI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SpeI Sse8387I SspI StuI SwaI
Van91I XhoI
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# ONE CUT ENZYMES ( 67 enzymes) #
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AccII 34 CGCG CG^CG
AccIII 2336 TCCGGA T^CCGGA
AclI 878 AACGTT AA^CGTT
AhdI 3025 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1972 CCTGAGG CC^T[ACGT]AGG
AseI 1389 ATTAAT AT^TAAT
AsnI 1389 ATTAAT AT^TAAT
AspEI 3025 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2658 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 41 CCCGGG C^[CT]CG[GA]G
BamHI 744 GGATCC G^GATCC
BanI 1364 GGCACC G^G[CT][GA]CC
BbsI 3602 GTCTTC GAAGAC(2/6)
BbvCI 2365 GCTGAGG CCTCAGC(-5/-2)
BcgI 1041 GCAGGACCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1542 AGATCT A^GATCT
BpuAI 3602 GTCTTC GAAGAC(2/6)
BsaAI 2900 TACGTA [CT]AC^GT[GA]
BsaI 3334 GAGACC GGTCTC(1/5)
BsaWI 2336 TCCGGA [AT]^CCGG[AT]
BseAI 2336 TCCGGA T^CCGGA
BsiEI 30 CGGCCG CG[GA][CT]^CG
BsoBI 41 CCCGGG C^[CT]CG[GA]G
Bsp1407I 386 TGTACA T^GTACA
BspEI 2336 TCCGGA T^CCGGA
BspHI 594 TCATGA T^CATGA
BspMI 1797 ACCTGC ACCTGC(4/8)
BsrBI 3523 GAGCGG CCGCTC(-3/-3)
BsrFI 1361 GCCGGC [GA]^CCGG[CT]
BsrGI 386 TGTACA T^GTACA
BstUI 34 CGCG CG^CG
Bsu36I 1972 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1361 GCCGGC [GA]^CCGG[CT]
EagI 30 CGGCCG C^GGCCG
Eam1105I 3025 GACACCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 30 CGGCCG C^GGCCG
Eco52I 30 CGGCCG C^GGCCG
Eco81I 1972 CCTGAGG CC^T[ACGT]AGG
EcoRI 2096 GAATTC G^AATTC
EcoT22I 3319 ATGCAT ATGCA^T
HincII 1473 GTTAAC GT[CT]^[GA]AC
HindII 1473 GTTAAC GT[CT]^[GA]AC
HpaI 1473 GTTAAC GTT^AAC
KspI 33 CCGCGG CCGC^GG
MroI 2336 TCCGGA T^CCGGA
MvnI 34 CGCG CG^CG
NaeI 1361 GCCGGC GCC^GGC
NdeI 221 CATATG CA^TATG
NgoMIV 1361 GCCGGC G^CCGGC
NheI 3696 GCTAGC G^CTAGC
NsiI 3319 ATGCAT ATGCA^T
PflFI 2658 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1389 ATTAAT AT^TAAT
Psp1406I 878 AACGTT AA^CGTT
RcaI 594 TCATGA T^CATGA
SacI 420 GAGCTC GAGCT^C
SacII 33 CCGCGG CCGC^GG
SexAI 1764 ACCTGGT A^CC[AT]GGT
SmaI 41 CCCGGG CCC^GGG
SnaBI 2900 TACGTA TAC^GTA
SphI 2603 GCATGC GCATG^C
SspBI 386 TGTACA T^GTACA
Tth111I 2658 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3606 TCTAGA T^CTAGA
XcmI 1673 CCAAGAAAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 41 CCCGGG C^CCGGG
XmaI 41 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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ApaI 25 GGGCCC GGGCC^C
3649 GGGCCC
Asp700I 206 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1174 GAAGTCTTTC
AspHI 420 GAGCTC G[AT]GC[AT]^C
1736 GTGCTC
BclI 950 TGATCA T^GATCA
1395 TGATCA
BlpI 483 GCTCAGC GC^T[ACGT]AGC
778 GCTAAGC
Bpu1102I 483 GCTCAGC GC^T[ACGT]AGC
778 GCTAAGC
BsiHKAI 420 GAGCTC G[AT]GC[AT]^C
1736 GTGCTC
BsrDI 248 CATTGC GCAATG(2/0)
844 GCAATG
BstXI 2354 CCAAGCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3791 CCAAGGAGTTGG
CelII 483 GCTCAGC GC^T[ACGT]AGC
778 GCTAAGC
EaeI 30 CGGCCG [CT]^GGCC[GA]
3804 TGGCCG
FbaI 950 TGATCA T^GATCA
1395 TGATCA
NcoI 740 CCATGG C^CATGG
3725 CCATGG
NspI 2603 GCATGC [GA]CATG^[CT]
2887 GCATGT
ScaI 1286 AGTACT AGT^ACT
3359 AGTACT
SmlI 3103 CTCAAG C^T[CT][GA]AG
3781 CTTGAG
TaqI 1050 TCGA T^CGA
3021 TCGA
TthHB8I 1050 TCGA T^CGA
3021 TCGA
XmnI 206 GAAATTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1174 GAAGTCTTTC
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BanII BbvI
BcnI BfaI BmyI BpmI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII DsaI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII