(Circular) (Six-base) MAPSORT of: pFN21AB4524.seq Check: 3991 from: 1 to: 8521 pFN21AB4524 8521 bp With 182 enzymes: SgfI * July 27, 2009 13:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3811 3811 Size: 8521 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6331 278 Size: 53 83 186 936 4795 2468 Fragments arranged by size: 4795 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5864 1937 Size: 3927 4594 Acc65I G'GTAC_C Cuts at: 5842 5842 Size: 8521 AclI AA'CG_TT Cuts at: 1563 2884 6648 7021 1563 Size: 1321 3764 373 3063 Fragments arranged by size: 3764 3063 1321 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2147 2580 2954 3152 3456 3536 4754 6584 Size: 515 433 374 198 304 80 1218 1830 Cuts at: 6584 7968 8236 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1830 1384 1218 515 433 374 304 268 198 80 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8324 AflIII A'CryG_T Cuts at: 1203 7420 8397 1203 Size: 6217 977 1327 Fragments arranged by size: 6217 1327 977 AgeI A'CCGG_T Cuts at: 7352 7352 Size: 8521 AhdI GACnn_n'nnGTC Cuts at: 5035 7250 5035 Size: 2215 6306 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8521 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3609 1184 Size: 2425 6096 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2976 3008 4257 4289 2976 Size: 32 1249 32 7208 Fragments arranged by size: 7208 1249 32 32 AlwNI CAG_nnn'CTG Cuts at: 2302 2896 3613 6264 7836 2302 Size: 594 717 2651 1572 2987 Fragments arranged by size: 2987 2651 1572 717 594 ApaI G_GGCC'C Cuts at: 2034 5658 2034 Size: 3624 4897 ApaLI G'TGCA_C Cuts at: 6578 7734 6578 Size: 1156 7365 ApoI r'AATT_y Cuts at: 1493 2117 2212 4101 5199 5227 5402 5830 6056 Size: 624 95 1889 1098 28 175 428 226 Cuts at: 6056 6208 8317 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1889 1697 1098 624 428 226 175 152 95 28 AseI AT'TA_AT Cuts at: 160 1032 3395 7075 160 Size: 872 2363 3680 1606 Fragments arranged by size: 3680 2363 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8521 AvaI C'yCGr_G Cuts at: 1870 1943 2046 5846 1870 Size: 73 103 3800 4545 Fragments arranged by size: 4545 3800 103 73 BamHI G'GATC_C Cuts at: 2749 5851 6218 2749 Size: 3102 367 5052 Fragments arranged by size: 5052 3102 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3369 5842 7297 618 Size: 325 217 627 1582 2473 1455 1842 Fragments arranged by size: 2473 1842 1582 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2034 2072 2429 5658 5840 1391 Size: 643 38 357 3229 182 4072 Fragments arranged by size: 4072 3229 643 357 182 38 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5600 928 Size: 971 3701 3849 Fragments arranged by size: 3849 3701 971 BbvCI CC'TCA_GC Cuts at: 4371 4371 Size: 8521 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3035 3069 6712 6746 1903 Size: 34 1098 34 3643 34 3678 Fragments arranged by size: 3678 3643 1098 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6413 7623 6413 Size: 1210 7311 BclI T'GATC_A Cuts at: 1548 2955 3400 1548 Size: 1407 445 6669 Fragments arranged by size: 6669 1407 445 BfrBI ATG'CAT Cuts at: 5326 5326 Size: 8521 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7132 136 Size: 107 122 71 631 605 5460 1525 Fragments arranged by size: 5460 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3547 8516 3547 Size: 4969 3552 BlpI GC'TnA_GC Cuts at: 2489 2784 5938 2489 Size: 295 3154 5072 Fragments arranged by size: 5072 3154 295 Bme1580I G_kGCm'C Cuts at: 1790 2034 4790 4797 5658 6582 7738 1790 Size: 244 2756 7 861 924 1156 2573 Fragments arranged by size: 2756 2573 1156 924 861 244 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8521 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4280 6252 7210 Size: 416 53 83 399 24 2863 1972 958 Cuts at: 7210 442 Size: 1753 Fragments arranged by size: 2863 1972 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 5705 1056 Size: 4649 3872 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2101 4396 5576 2101 Size: 2295 1180 5046 Fragments arranged by size: 5046 2295 1180 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2171 2519 3359 3927 4035 4149 4661 4960 Size: 932 348 840 568 108 114 512 299 Cuts at: 4960 7181 1239 Size: 2221 2579 Fragments arranged by size: 2579 2221 932 840 568 512 348 299 114 108 Bpu10I CC'TnA_GC Cuts at: 4371 4371 Size: 8521 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5093 5807 6646 7511 7809 5093 Size: 714 839 865 298 5805 Fragments arranged by size: 5805 865 839 714 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5333 7184 882 Size: 617 3834 1851 2219 Fragments arranged by size: 3834 2219 1851 617 BsaAI yAC'GTr Cuts at: 493 1204 4907 6312 493 Size: 711 3703 1405 2702 Fragments arranged by size: 3703 2702 1405 711 BsaBI GATnn'nnATC Cuts at: 8515 8515 Size: 8521 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6328 6710 7345 275 Size: 53 83 186 936 4795 382 635 1451 Fragments arranged by size: 4795 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4341 6954 7352 7626 7773 8184 4341 Size: 2613 398 274 147 411 4678 Fragments arranged by size: 4678 2613 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2363 2393 2447 2477 3706 Size: 30 165 30 960 30 54 30 1229 Cuts at: 3706 3736 3811 3814 3841 3844 3943 3973 1178 Size: 30 75 3 27 3 99 30 5726 Fragments arranged by size: 5726 1229 960 165 99 75 54 30 30 30 30 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2339 3816 3819 3822 3825 3828 3831 3834 Size: 1153 1477 3 3 3 3 3 3 Cuts at: 3834 3837 3840 3843 3877 4029 4107 4140 4143 Size: 3 3 3 34 152 78 33 3 Cuts at: 4143 4182 4913 1186 Size: 39 731 4794 Fragments arranged by size: 4794 1477 1153 731 152 78 39 34 33 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2621 3630 5233 5356 7724 8220 2621 Size: 1009 1603 123 2368 496 2922 Fragments arranged by size: 2922 2368 1603 1009 496 123 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3317 4355 5706 1163 Size: 573 1581 1038 1351 3978 Fragments arranged by size: 3978 1581 1351 1038 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2038 5971 6732 7760 1540 Size: 275 186 37 3933 761 1028 2301 Fragments arranged by size: 3933 2301 1028 761 275 186 37 BsiHKAI G_wGCw'C Cuts at: 2429 3745 6582 6667 7738 2429 Size: 1316 2837 85 1071 3212 Fragments arranged by size: 3212 2837 1316 1071 85 BsmI GAATG_Cn' Cuts at: 3574 4146 4215 4972 5326 5578 5636 6032 6125 Size: 572 69 757 354 252 58 396 93 Cuts at: 6125 3574 Size: 5970 Fragments arranged by size: 5970 757 572 396 354 252 93 69 58 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2034 2072 2429 3745 4790 4797 5658 Size: 399 244 38 357 1316 1045 7 861 Cuts at: 5658 5840 6582 6667 7738 1391 Size: 182 742 85 1071 2174 Fragments arranged by size: 2174 1316 1071 1045 861 742 399 357 244 182 85 38 7 BspEI T'CCGG_A Cuts at: 4341 8184 4341 Size: 3843 4678 BspHI T'CATG_A Cuts at: 2599 6410 8140 2599 Size: 3811 1730 2980 Fragments arranged by size: 3811 2980 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3811 5876 844 Size: 2967 2065 3489 Fragments arranged by size: 3489 2967 2065 BsrBI CCG'CTC Cuts at: 1394 1823 5530 6408 1394 Size: 429 3707 878 3507 Fragments arranged by size: 3707 3507 878 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2252 2856 7016 7190 65 Size: 1183 1004 604 4160 174 1396 Fragments arranged by size: 4160 1396 1183 1004 604 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3366 7165 7352 1700 Size: 110 1556 3799 187 2869 Fragments arranged by size: 3799 2869 1556 187 110 BsrGI T'GTAC_A Cuts at: 96 2391 96 Size: 2295 6226 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8521 BssSI C'ACGA_G Cuts at: 6581 7593 6581 Size: 1012 7509 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8521 BstBI TT'CG_AA Cuts at: 7325 7325 Size: 8521 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8521 BstXI CCAn_nnnn'nTGG Cuts at: 4366 5803 4366 Size: 1437 7084 BstYI r'GATC_y Cuts at: 1972 2749 3547 5413 5851 6218 6603 6620 8072 Size: 777 798 1866 438 367 385 17 1452 Cuts at: 8072 8158 8170 8516 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1866 1452 798 777 438 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3978 3978 Size: 8521 BtgI C'CryG_G Cuts at: 513 1065 2004 2038 2745 5730 513 Size: 552 939 34 707 2985 3304 Fragments arranged by size: 3304 2985 939 707 552 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5598 487 Size: 683 56 4372 3410 Fragments arranged by size: 4372 3410 683 56 BtsI GCAGTG_nn' Cuts at: 3569 4884 6037 6831 6851 3569 Size: 1315 1153 794 20 5239 Fragments arranged by size: 5239 1315 1153 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8521 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3846 3881 4065 4100 5767 5802 529 Size: 35 3282 35 184 35 1667 35 3248 Fragments arranged by size: 3282 3248 1667 184 35 35 35 35 DraI TTT'AAA Cuts at: 5825 5825 Size: 8521 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7528 809 Size: 507 6212 1802 Fragments arranged by size: 6212 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2035 5809 5968 Size: 54 1425 50 275 18 205 3774 159 Cuts at: 5968 6857 8486 8 Size: 889 1629 43 Fragments arranged by size: 3774 1629 1425 889 275 205 159 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2035 5968 1537 Size: 275 223 3933 4090 Fragments arranged by size: 4090 3933 275 223 EarI CTCTTCn'nnn_ Cuts at: 1353 2607 3108 4009 4167 4832 5543 5642 6451 Size: 1254 501 901 158 665 711 99 809 Cuts at: 6451 1353 Size: 3423 Fragments arranged by size: 3423 1254 901 809 711 665 501 158 99 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7108 7482 7628 387 Size: 1064 138 5519 374 146 1280 Fragments arranged by size: 5519 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2427 2427 Size: 8521 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 2171 2519 2580 2954 3152 3359 Size: 393 515 24 348 61 374 198 207 Cuts at: 3359 3456 3536 3927 4035 4149 4661 4754 4960 Size: 97 80 391 108 114 512 93 206 Cuts at: 4960 6584 7181 7968 8236 1239 Size: 1624 597 787 268 1524 Fragments arranged by size: 1624 1524 787 597 515 512 393 391 374 348 268 207 206 198 114 108 97 93 80 61 24 EcoNI CCTnn'n_nnAGG Cuts at: 2321 4382 5348 2321 Size: 2061 966 5494 Fragments arranged by size: 5494 2061 966 EcoO109I rG'GnC_Cy Cuts at: 2030 2043 3049 3610 4208 4375 5654 5655 2030 Size: 13 1006 561 598 167 1279 1 4896 Fragments arranged by size: 4896 1279 1006 598 561 167 13 1 EcoRI G'AATT_C Cuts at: 4101 5830 6208 4101 Size: 1729 378 6414 Fragments arranged by size: 6414 1729 378 FspI TGC'GCA Cuts at: 7027 7027 Size: 8521 HaeII r_GCGC'y Cuts at: 1678 7668 1678 Size: 5990 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2363 2395 2447 2479 2947 Size: 32 135 32 791 32 52 32 468 Cuts at: 2947 2979 2988 3020 4862 4894 5021 5053 5590 Size: 32 9 32 1842 32 127 32 537 Cuts at: 5590 5622 7162 7194 7236 7268 1373 Size: 32 1540 32 42 32 2626 Fragments arranged by size: 2626 1842 1540 791 537 468 135 127 52 42 32 32 32 32 32 32 32 32 32 32 32 9 HincII GTy'rAC Cuts at: 669 1657 1938 3480 5865 6111 669 Size: 988 281 1542 2385 246 3079 Fragments arranged by size: 3079 2385 1542 988 281 246 HindIII A'AGCT_T Cuts at: 748 2670 3539 4316 4361 5021 748 Size: 1922 869 777 45 660 4248 Fragments arranged by size: 4248 1922 869 777 660 45 HpaI GTT'AAC Cuts at: 3480 6111 3480 Size: 2631 5890 KpnI G_GTAC'C Cuts at: 5846 5846 Size: 8521 MfeI C'AATT_G Cuts at: 6120 6120 Size: 8521 MluI A'CGCG_T Cuts at: 8397 8397 Size: 8521 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2688 2920 3447 4539 4933 5665 7635 7819 Size: 1383 232 527 1092 394 732 1970 184 Cuts at: 7819 1305 Size: 2007 Fragments arranged by size: 2007 1970 1383 1092 732 527 394 232 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6699 Fragments arranged by size: 6699 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3155 3161 5567 6479 6838 6997 518 Size: 648 1989 6 2406 912 359 159 2042 Fragments arranged by size: 2406 2042 1989 912 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2040 2493 2896 2953 2978 5237 5937 6614 Size: 434 453 403 57 25 2259 700 677 Cuts at: 6614 7762 8007 1606 Size: 1148 245 2120 Fragments arranged by size: 2259 2120 1148 700 677 453 434 403 245 57 25 NaeI GCC'GGC Cuts at: 3368 3368 Size: 8521 NcoI C'CATG_G Cuts at: 513 1065 2004 2745 5730 513 Size: 552 939 741 2985 3304 Fragments arranged by size: 3304 2985 939 741 552 NdeI CA'TA_TG Cuts at: 387 2227 387 Size: 1840 6681 NgoMIV G'CCGG_C Cuts at: 3366 3366 Size: 8521 NheI G'CTAG_C Cuts at: 1052 5701 1052 Size: 4649 3872 NotI GC'GGCC_GC Cuts at: 5968 5968 Size: 8521 NsiI A_TGCA'T Cuts at: 5328 5328 Size: 8521 NspI r_CATG'y Cuts at: 1132 1229 4612 4896 5879 7424 1132 Size: 97 3383 284 983 1545 2229 Fragments arranged by size: 3383 2229 1545 983 284 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8521 PciI A'CATG_T Cuts at: 7420 7420 Size: 8521 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8521 PfoI T'CCnGG_A Cuts at: 4174 4201 4638 4174 Size: 27 437 8057 Fragments arranged by size: 8057 437 27 PmeI GTTT'AAAC Cuts at: 5825 5825 Size: 8521 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6590 6622 8103 8135 6590 Size: 32 1481 32 6976 Fragments arranged by size: 6976 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2043 3049 3610 4375 2043 Size: 1006 561 765 6189 Fragments arranged by size: 6189 1006 765 561 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8521 PsiI TTA'TAA Cuts at: 4323 6091 4323 Size: 1768 6753 PspOMI G'GGCC_C Cuts at: 2030 5654 2030 Size: 3624 4897 PstI C_TGCA'G Cuts at: 830 3249 3451 4282 5625 5873 830 Size: 2419 202 831 1343 248 3478 Fragments arranged by size: 3478 2419 1343 831 248 202 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8521 PvuII CAG'CTG Cuts at: 2493 2953 5237 2493 Size: 460 2284 5777 Fragments arranged by size: 5777 2284 460 SacI G_AGCT'C Cuts at: 2429 2429 Size: 8521 SacII CC_GC'GG Cuts at: 2041 2041 Size: 8521 SalI G'TCGA_C Cuts at: 1936 5863 1936 Size: 3927 4594 SanDI GG'GwC_CC Cuts at: 2043 2043 Size: 8521 SbfI CC_TGCA'GG Cuts at: 5873 5873 Size: 8521 ScaI AGT'ACT Cuts at: 3293 5366 6769 8481 3293 Size: 2073 1403 1712 3333 Fragments arranged by size: 3333 2073 1712 1403 SexAI A'CCwGG_T Cuts at: 3769 3769 Size: 8521 SfcI C'TryA_G Cuts at: 826 1045 2948 3245 3447 3497 4278 5621 5869 Size: 219 1903 297 202 50 781 1343 248 Cuts at: 5869 7004 7685 7876 826 Size: 1135 681 191 1471 Fragments arranged by size: 1903 1471 1343 1135 781 681 297 248 219 202 191 50 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8521 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8521 SmaI CCC'GGG Cuts at: 1872 2048 5848 1872 Size: 176 3800 4545 Fragments arranged by size: 4545 3800 176 SmlI C'TyrA_G Cuts at: 820 1017 1943 5108 5786 6625 7526 7788 820 Size: 197 926 3165 678 839 901 262 1553 Fragments arranged by size: 3165 1553 926 901 839 678 262 197 SnaBI TAC'GTA Cuts at: 493 4907 6312 493 Size: 4414 1405 2702 Fragments arranged by size: 4414 2702 1405 SpeI A'CTAG_T Cuts at: 152 152 Size: 8521 SphI G_CATG'C Cuts at: 1132 4612 5879 1132 Size: 3480 1267 3774 Fragments arranged by size: 3774 3480 1267 SspI AAT'ATT Cuts at: 5 52 6445 5 Size: 47 6393 2081 Fragments arranged by size: 6393 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8521 StyI C'CwwG_G Cuts at: 513 1065 2004 2400 2745 5508 5718 5730 5748 Size: 552 939 396 345 2763 210 12 18 Cuts at: 5748 5796 5960 513 Size: 48 164 3074 Fragments arranged by size: 3074 2763 939 552 396 345 210 164 48 18 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2530 6548 6565 6718 6903 7348 325 Size: 1255 950 4018 17 153 185 445 1498 Fragments arranged by size: 4018 1498 1255 950 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2391 3291 5364 Size: 274 80 33 51 1443 414 900 2073 Cuts at: 5364 6767 8479 96 Size: 1403 1712 138 Fragments arranged by size: 2073 1712 1443 1403 900 414 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3221 3253 3775 3807 3814 3846 8481 8513 3221 Size: 32 522 32 7 32 4635 32 3229 Fragments arranged by size: 4635 3229 522 32 32 32 32 7 Tth111I GACn'n_nGTC Cuts at: 1316 4666 1316 Size: 3350 5171 XbaI T'CTAG_A Cuts at: 5611 5857 5611 Size: 246 8275 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3685 3685 Size: 8521 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8521 XmaI C'CCGG_G Cuts at: 1870 2046 5846 1870 Size: 176 3800 4545 Fragments arranged by size: 4545 3800 176 XmnI GAAnn'nnTTC Cuts at: 2215 3183 6650 2215 Size: 968 3467 4086 Fragments arranged by size: 4086 3467 968 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6329 276 Size: 53 83 186 936 4795 2468 Fragments arranged by size: 4795 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PspXI PsrI RsrII SapI SfiI SfoI SrfI SwaI