(Circular) (Six-base) MAPSORT of: pFN21AB4523.seq Check: 4672 from: 1 to: 6220 pFN21AB4523 6220 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2216 2964 2216 Size: 748 5472 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4030 278 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2553 3563 1937 Size: 616 1010 4594 Fragments arranged by size: 4594 1010 616 Acc65I G'GTAC_C Cuts at: 3541 3541 Size: 6220 AclI AA'CG_TT Cuts at: 1563 4347 4720 1563 Size: 2784 373 3063 Fragments arranged by size: 3063 2784 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3174 4283 5667 5935 1632 Size: 1542 1109 1384 268 1917 Fragments arranged by size: 1917 1542 1384 1109 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6023 AflIII A'CryG_T Cuts at: 1203 2136 2138 5119 6096 1203 Size: 933 2 2981 977 1327 Fragments arranged by size: 2981 1327 977 933 2 AgeI A'CCGG_T Cuts at: 2889 5051 2889 Size: 2162 4058 AhdI GACnn_n'nnGTC Cuts at: 3013 4949 3013 Size: 1936 4284 AleI CACnn'nnGTG Cuts at: 1166 2836 1166 Size: 1670 4550 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6220 AlwNI CAG_nnn'CTG Cuts at: 2098 2488 2576 3095 3358 3963 5535 2098 Size: 390 88 519 263 605 1572 2783 Fragments arranged by size: 2783 1572 605 519 390 263 88 ApaI G_GGCC'C Cuts at: 2082 2375 2082 Size: 293 5927 ApaLI G'TGCA_C Cuts at: 2110 4277 5433 2110 Size: 2167 1156 2897 Fragments arranged by size: 2897 2167 1156 ApoI r'AATT_y Cuts at: 1493 3529 3755 3907 6016 1493 Size: 2036 226 152 2109 1697 Fragments arranged by size: 2109 2036 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3174 4774 160 Size: 872 2142 1600 1606 Fragments arranged by size: 2142 1606 1600 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 AvaI C'yCGr_G Cuts at: 1870 1943 2082 3221 3545 1870 Size: 73 139 1139 324 4545 Fragments arranged by size: 4545 1139 324 139 73 BamHI G'GATC_C Cuts at: 3550 3917 3550 Size: 367 5853 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2326 2822 2975 3541 4996 Size: 325 217 627 539 496 153 566 1455 Cuts at: 4996 618 Size: 1842 Fragments arranged by size: 1842 1455 627 566 539 496 325 217 153 BanII G_rGCy'C Cuts at: 1391 2082 2264 2375 3539 1391 Size: 691 182 111 1164 4072 Fragments arranged by size: 4072 1164 691 182 111 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3485 3513 928 Size: 971 1586 28 3635 Fragments arranged by size: 3635 1586 971 28 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2296 2330 4411 4445 1903 Size: 34 359 34 2081 34 3678 Fragments arranged by size: 3678 2081 359 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4112 5322 4112 Size: 1210 5010 BclI T'GATC_A Cuts at: 1548 3349 1548 Size: 1801 4419 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4831 136 Size: 107 122 71 631 605 3159 1525 Fragments arranged by size: 3159 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6215 6215 Size: 6220 BlpI GC'TnA_GC Cuts at: 3494 3637 3494 Size: 143 6077 Bme1580I G_kGCm'C Cuts at: 1790 2082 2114 2331 2375 2978 3309 4281 5437 Size: 292 32 217 44 603 331 972 1156 Cuts at: 5437 1790 Size: 2573 Fragments arranged by size: 2573 1156 972 603 331 292 217 44 32 BmgBI CAC'GTC Cuts at: 1318 3511 1318 Size: 2193 4027 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2325 3951 4909 Size: 416 53 83 399 24 908 1626 958 Cuts at: 4909 442 Size: 1753 Fragments arranged by size: 1753 1626 958 908 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6220 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2108 2525 2701 3282 4880 1239 Size: 869 417 176 581 1598 2579 Fragments arranged by size: 2579 1598 869 581 417 176 Bpu10I CC'TnA_GC Cuts at: 2258 2352 2581 2258 Size: 94 229 5897 Fragments arranged by size: 5897 229 94 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2327 2341 4345 5210 5508 2327 Size: 14 2004 865 298 3039 Fragments arranged by size: 3039 2004 865 298 14 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4883 882 Size: 617 3384 2219 Fragments arranged by size: 3384 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2139 4011 493 Size: 711 935 1872 2702 Fragments arranged by size: 2702 1872 935 711 BsaBI GATnn'nnATC Cuts at: 6214 6214 Size: 6220 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4027 4409 5044 275 Size: 53 83 186 936 2494 382 635 1451 Fragments arranged by size: 2494 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2889 4653 5051 5325 5472 5883 2889 Size: 1764 398 274 147 411 3226 Fragments arranged by size: 3226 1764 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2546 2576 2674 2704 3176 Size: 30 165 30 1143 30 98 30 472 Cuts at: 3176 3206 1178 Size: 30 4192 Fragments arranged by size: 4192 1143 472 165 98 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2017 3279 1186 Size: 831 1262 4127 Fragments arranged by size: 4127 1262 831 BseYI C'CCAG_C Cuts at: 3489 5423 5919 3489 Size: 1934 496 3790 Fragments arranged by size: 3790 1934 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2942 3521 1163 Size: 573 1206 579 3862 Fragments arranged by size: 3862 1206 579 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2019 3670 4431 5459 1540 Size: 275 186 18 1651 761 1028 2301 Fragments arranged by size: 2301 1651 1028 761 275 186 18 BsiHKAI G_wGCw'C Cuts at: 2114 2196 2264 2358 2865 4281 4366 5437 2114 Size: 82 68 94 507 1416 85 1071 2897 Fragments arranged by size: 2897 1416 1071 507 94 85 82 68 BsmI GAATG_Cn' Cuts at: 3731 3824 3731 Size: 93 6127 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2114 2196 2264 2331 2358 2375 Size: 399 292 32 82 68 67 27 17 Cuts at: 2375 2865 2978 3309 3539 4281 4366 5437 1391 Size: 490 113 331 230 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 490 399 331 292 230 113 85 82 68 67 32 27 17 BspEI T'CCGG_A Cuts at: 5883 5883 Size: 6220 BspHI T'CATG_A Cuts at: 2857 3082 4109 5839 2857 Size: 225 1027 1730 3238 Fragments arranged by size: 3238 1730 1027 225 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2216 2280 2964 3575 844 Size: 1372 64 684 611 3489 Fragments arranged by size: 3489 1372 684 611 64 BsrBI CCG'CTC Cuts at: 1394 1823 4107 1394 Size: 429 2284 3507 Fragments arranged by size: 3507 2284 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3108 4715 4889 65 Size: 1183 1860 1607 174 1396 Fragments arranged by size: 1860 1607 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2060 2399 2889 4864 5051 1700 Size: 110 250 339 490 1975 187 2869 Fragments arranged by size: 2869 1975 490 339 250 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6220 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6220 BssSI C'ACGA_G Cuts at: 2718 4280 5292 2718 Size: 1562 1012 3646 Fragments arranged by size: 3646 1562 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2118 2200 794 Size: 1324 82 4814 Fragments arranged by size: 4814 1324 82 BstBI TT'CG_AA Cuts at: 5024 5024 Size: 6220 BstEII G'GTnAC_C Cuts at: 1184 2336 2586 1184 Size: 1152 250 4818 Fragments arranged by size: 4818 1152 250 BstYI r'GATC_y Cuts at: 1972 2500 2545 3550 3917 4302 4319 5771 5857 Size: 528 45 1005 367 385 17 1452 86 Cuts at: 5857 5869 6215 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1005 528 385 367 346 86 45 17 12 Bsu36I CC'TnA_GG Cuts at: 2569 2569 Size: 6220 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4729 Fragments arranged by size: 4729 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2380 487 Size: 683 56 1154 4327 Fragments arranged by size: 4327 1154 683 56 BtsI GCAGTG_nn' Cuts at: 2284 3398 3736 4530 4550 2284 Size: 1114 338 794 20 3954 Fragments arranged by size: 3954 1114 794 338 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6220 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6185 DraI TTT'AAA Cuts at: 3199 3524 3199 Size: 325 5895 DraIII CAC_nnn'GTG Cuts at: 2200 2200 Size: 6220 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5227 809 Size: 507 3911 1802 Fragments arranged by size: 3911 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 3667 4556 Size: 54 1425 50 275 18 186 1651 889 Cuts at: 4556 6185 8 Size: 1629 43 Fragments arranged by size: 1651 1629 1425 889 275 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2016 3667 1537 Size: 275 204 1651 4090 Fragments arranged by size: 4090 1651 275 204 EarI CTCTTCn'nnn_ Cuts at: 1353 2719 4150 1353 Size: 1366 1431 3423 Fragments arranged by size: 3423 1431 1366 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4807 5181 5327 387 Size: 1064 138 435 2783 374 146 1280 Fragments arranged by size: 2783 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2262 2262 Size: 6220 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2108 2525 2701 3174 3282 4283 4880 Size: 393 476 417 176 473 108 1001 597 Cuts at: 4880 5667 5935 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1001 787 597 476 473 417 393 268 176 108 EcoO109I rG'GnC_Cy Cuts at: 2079 2154 2269 2371 2868 2079 Size: 75 115 102 497 5431 Fragments arranged by size: 5431 497 115 102 75 EcoRI G'AATT_C Cuts at: 3529 3907 3529 Size: 378 5842 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2944 2944 Size: 6220 FspI TGC'GCA Cuts at: 2204 2276 3470 4726 2204 Size: 72 1194 1256 3698 Fragments arranged by size: 3698 1256 1194 72 FspAI rTGC'GCAy Cuts at: 2204 2204 Size: 6220 HaeII r_GCGC'y Cuts at: 1678 5367 1678 Size: 3689 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 2852 2884 2999 Size: 32 135 32 773 32 475 32 115 Cuts at: 2999 3031 4861 4893 4935 4967 1373 Size: 32 1830 32 42 32 2626 Fragments arranged by size: 2626 1830 773 475 135 115 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3564 3810 669 Size: 988 281 1626 246 3079 Fragments arranged by size: 3079 1626 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6220 HpaI GTT'AAC Cuts at: 3810 3810 Size: 6220 KpnI G_GTAC'C Cuts at: 3545 3545 Size: 6220 MfeI C'AATT_G Cuts at: 2904 3819 2904 Size: 915 5305 MluI A'CGCG_T Cuts at: 6096 6096 Size: 6220 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5334 5518 1305 Size: 4029 184 2007 Fragments arranged by size: 4029 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4398 Fragments arranged by size: 4398 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2463 2836 3029 3176 4178 4537 4696 Size: 648 1297 373 193 147 1002 359 159 Cuts at: 4696 518 Size: 2042 Fragments arranged by size: 2042 1297 1002 648 373 359 193 159 147 MspA1I CmG'CkG Cuts at: 1606 2067 2118 2679 2836 3286 3636 4313 5461 Size: 461 51 561 157 450 350 677 1148 Cuts at: 5461 5706 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 561 461 450 350 245 157 51 NaeI GCC'GGC Cuts at: 2062 2401 2062 Size: 339 5881 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4729 Fragments arranged by size: 4729 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6220 NgoMIV G'CCGG_C Cuts at: 2060 2399 2060 Size: 339 5881 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6220 NotI GC'GGCC_GC Cuts at: 3667 3667 Size: 6220 NspI r_CATG'y Cuts at: 1132 1229 3049 3578 5123 1132 Size: 97 1820 529 1545 2229 Fragments arranged by size: 2229 1820 1545 529 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6220 PciI A'CATG_T Cuts at: 5119 5119 Size: 6220 PflMI CCAn_nnn'nTGG Cuts at: 1108 2488 3381 1108 Size: 1380 893 3947 Fragments arranged by size: 3947 1380 893 PfoI T'CCnGG_A Cuts at: 2541 2541 Size: 6220 PmeI GTTT'AAAC Cuts at: 3524 3524 Size: 6220 PmlI CAC'GTG Cuts at: 2139 2139 Size: 6220 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4289 4321 5802 5834 4289 Size: 32 1481 32 4675 Fragments arranged by size: 4675 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2269 2868 2269 Size: 599 5621 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6220 PsiI TTA'TAA Cuts at: 3790 3790 Size: 6220 PspOMI G'GGCC_C Cuts at: 2078 2371 2078 Size: 293 5927 PstI C_TGCA'G Cuts at: 830 3572 830 Size: 2742 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6220 PvuII CAG'CTG Cuts at: 2118 2836 3286 2118 Size: 718 450 5052 Fragments arranged by size: 5052 718 450 SacI G_AGCT'C Cuts at: 2264 2264 Size: 6220 SalI G'TCGA_C Cuts at: 1936 3562 1936 Size: 1626 4594 SanDI GG'GwC_CC Cuts at: 2868 2868 Size: 6220 SbfI CC_TGCA'GG Cuts at: 3572 3572 Size: 6220 ScaI AGT'ACT Cuts at: 3159 4468 6180 3159 Size: 1309 1712 3199 Fragments arranged by size: 3199 1712 1309 SfcI C'TryA_G Cuts at: 826 1045 3568 4703 5384 5575 826 Size: 219 2523 1135 681 191 1471 Fragments arranged by size: 2523 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6220 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6220 SmaI CCC'GGG Cuts at: 1872 2084 3547 1872 Size: 212 1463 4545 Fragments arranged by size: 4545 1463 212 SmlI C'TyrA_G Cuts at: 820 1017 1943 2320 2342 4324 5225 5487 820 Size: 197 926 377 22 1982 901 262 1553 Fragments arranged by size: 1982 1553 926 901 377 262 197 22 SnaBI TAC'GTA Cuts at: 493 4011 493 Size: 3518 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6220 SphI G_CATG'C Cuts at: 1132 3578 1132 Size: 2446 3774 SspI AAT'ATT Cuts at: 5 52 2709 4144 5 Size: 47 2657 1435 2081 Fragments arranged by size: 2657 2081 1435 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6220 StyI C'CwwG_G Cuts at: 513 1065 2004 2788 2980 3659 513 Size: 552 939 784 192 679 3074 Fragments arranged by size: 3074 939 784 679 552 192 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2297 2993 4247 4264 4417 4602 5047 Size: 1255 717 696 1254 17 153 185 445 Cuts at: 5047 325 Size: 1498 Fragments arranged by size: 1498 1255 1254 717 696 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3157 4466 6178 Size: 274 80 33 51 1443 1180 1309 1712 Cuts at: 6178 96 Size: 138 Fragments arranged by size: 1712 1443 1309 1180 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2674 2706 3176 3208 6180 6212 2674 Size: 32 470 32 2972 32 2682 Fragments arranged by size: 2972 2682 470 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6220 XbaI T'CTAG_A Cuts at: 3556 3556 Size: 6220 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2176 2224 2987 3061 3142 2176 Size: 48 763 74 81 5254 Fragments arranged by size: 5254 763 81 74 48 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6220 XmaI C'CCGG_G Cuts at: 1870 2082 3545 1870 Size: 212 1463 4545 Fragments arranged by size: 4545 1463 212 XmnI GAAnn'nnTTC Cuts at: 2246 2708 4349 2246 Size: 462 1641 4117 Fragments arranged by size: 4117 1641 462 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4028 276 Size: 53 83 186 936 2494 2468 Fragments arranged by size: 2494 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI EcoRV FseI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI