Restriction map for
pFN21AB4523
(Linear) (Six-base) MAPSORT of: KIBB4523.seq Check: 6169 from: 1 to: 1515
KIBB4523.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 211 959 1515
Size: 211 748 556
Fragments arranged by size:
748 556 211
AccI GT'mk_AC
Cuts at: 0 548 1515
Size: 548 967
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1169 1515
Size: 1169 346
AflIII A'CryG_T
Cuts at: 0 131 133 1515
Size: 131 2 1382
Fragments arranged by size:
1382 131 2
AgeI A'CCGG_T
Cuts at: 0 884 1515
Size: 884 631
AhdI GACnn_n'nnGTC
Cuts at: 0 1008 1515
Size: 1008 507
AleI CACnn'nnGTG
Cuts at: 0 831 1515
Size: 831 684
AlwNI CAG_nnn'CTG
Cuts at: 0 93 483 571 1090 1353 1515
Size: 93 390 88 519 263 162
Fragments arranged by size:
519 390 263 162 93 88
ApaI G_GGCC'C
Cuts at: 0 77 370 1515
Size: 77 293 1145
Fragments arranged by size:
1145 293 77
ApaLI G'TGCA_C
Cuts at: 0 105 1515
Size: 105 1410
AseI AT'TA_AT
Cuts at: 0 1169 1515
Size: 1169 346
AvaI C'yCGr_G
Cuts at: 0 77 1216 1515
Size: 77 1139 299
Fragments arranged by size:
1139 299 77
BanI G'GyrC_C
Cuts at: 0 321 817 970 1515
Size: 321 496 153 545
Fragments arranged by size:
545 496 321 153
BanII G_rGCy'C
Cuts at: 0 77 259 370 1515
Size: 77 182 111 1145
Fragments arranged by size:
1145 182 111 77
BbsI GAAGACnn'nnnn_
Cuts at: 0 1480 1508 1515
Size: 1480 28 7
Fragments arranged by size:
1480 28 7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 291 325 1515
Size: 291 34 1190
Fragments arranged by size:
1190 291 34
BclI T'GATC_A
Cuts at: 0 1344 1515
Size: 1344 171
BlpI GC'TnA_GC
Cuts at: 0 1489 1515
Size: 1489 26
Bme1580I G_kGCm'C
Cuts at: 0 77 109 326 370 973 1304 1515
Size: 77 32 217 44 603 331 211
Fragments arranged by size:
603 331 217 211 77 44 32
BmgBI CAC'GTC
Cuts at: 0 1506 1515
Size: 1506 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 320 1515
Size: 320 1195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 520 696 1277 1515
Size: 103 417 176 581 238
Fragments arranged by size:
581 417 238 176 103
Bpu10I CC'TnA_GC
Cuts at: 0 253 347 576 1515
Size: 253 94 229 939
Fragments arranged by size:
939 253 229 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 336 1515
Size: 322 14 1179
Fragments arranged by size:
1179 322 14
BsaAI yAC'GTr
Cuts at: 0 134 1515
Size: 134 1381
BsaWI w'CCGG_w
Cuts at: 0 884 1515
Size: 884 631
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 541 571 669 699 1171 1201 1515
Size: 541 30 98 30 472 30 314
Fragments arranged by size:
541 472 314 98 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 12 1274 1515
Size: 12 1262 241
Fragments arranged by size:
1262 241 12
BseYI C'CCAG_C
Cuts at: 0 1484 1515
Size: 1484 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 1515
Size: 937 578
BsiEI CG_ry'CG
Cuts at: 0 14 1515
Size: 14 1501
BsiHKAI G_wGCw'C
Cuts at: 0 109 191 259 353 860 1515
Size: 109 82 68 94 507 655
Fragments arranged by size:
655 507 109 94 82 68
Bsp1286I G_dGCh'C
Cuts at: 0 77 109 191 259 326 353 370 860
Size: 77 32 82 68 67 27 17 490
Cuts at: 860 973 1304 1515
Size: 113 331 211
Fragments arranged by size:
490 331 211 113 82 77 68 67
32 27 17
BspHI T'CATG_A
Cuts at: 0 852 1077 1515
Size: 852 225 438
Fragments arranged by size:
852 438 225
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 211 275 959 1515
Size: 211 64 684 556
Fragments arranged by size:
684 556 211 64
BsrDI GCAATG_nn'
Cuts at: 0 1103 1515
Size: 1103 412
BsrFI r'CCGG_y
Cuts at: 0 55 394 884 1515
Size: 55 339 490 631
Fragments arranged by size:
631 490 339 55
BssSI C'ACGA_G
Cuts at: 0 713 1515
Size: 713 802
BstAPI GCAn_nnn'nTGC
Cuts at: 0 113 195 1515
Size: 113 82 1320
Fragments arranged by size:
1320 113 82
BstEII G'GTnAC_C
Cuts at: 0 331 581 1515
Size: 331 250 934
Fragments arranged by size:
934 331 250
BstYI r'GATC_y
Cuts at: 0 495 540 1515
Size: 495 45 975
Fragments arranged by size:
975 495 45
Bsu36I CC'TnA_GG
Cuts at: 0 564 1515
Size: 564 951
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 375 1515
Size: 375 1140
BtsI GCAGTG_nn'
Cuts at: 0 279 1393 1515
Size: 279 1114 122
Fragments arranged by size:
1114 279 122
DraI TTT'AAA
Cuts at: 0 1194 1515
Size: 1194 321
DraIII CAC_nnn'GTG
Cuts at: 0 195 1515
Size: 195 1320
EaeI y'GGCC_r
Cuts at: 0 11 1515
Size: 11 1504
EagI C'GGCC_G
Cuts at: 0 11 1515
Size: 11 1504
EarI CTCTTCn'nnn_
Cuts at: 0 714 1515
Size: 714 801
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1515
Size: 19 1496
EcoICRI GAG'CTC
Cuts at: 0 257 1515
Size: 257 1258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 520 696 1169 1277 1515
Size: 103 417 176 473 108 238
Fragments arranged by size:
473 417 238 176 108 103
EcoO109I rG'GnC_Cy
Cuts at: 0 74 149 264 366 863 1515
Size: 74 75 115 102 497 652
Fragments arranged by size:
652 497 115 102 75 74
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 939 1515
Size: 939 576
FspI TGC'GCA
Cuts at: 0 199 271 1465 1515
Size: 199 72 1194 50
Fragments arranged by size:
1194 199 72 50
FspAI rTGC'GCAy
Cuts at: 0 199 1515
Size: 199 1316
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 847 879 994 1026 1515
Size: 340 32 475 32 115 32 489
Fragments arranged by size:
489 475 340 115 32 32 32
MfeI C'AATT_G
Cuts at: 0 899 1515
Size: 899 616
MslI CAynn'nnrTG
Cuts at: 0 458 831 1024 1171 1515
Size: 458 373 193 147 344
Fragments arranged by size:
458 373 344 193 147
MspA1I CmG'CkG
Cuts at: 0 62 113 674 831 1281 1515
Size: 62 51 561 157 450 234
Fragments arranged by size:
561 450 234 157 62 51
NaeI GCC'GGC
Cuts at: 0 57 396 1515
Size: 57 339 1119
Fragments arranged by size:
1119 339 57
NgoMIV G'CCGG_C
Cuts at: 0 55 394 1515
Size: 55 339 1121
Fragments arranged by size:
1121 339 55
NspI r_CATG'y
Cuts at: 0 1044 1515
Size: 1044 471
PflMI CCAn_nnn'nTGG
Cuts at: 0 483 1376 1515
Size: 483 893 139
Fragments arranged by size:
893 483 139
PfoI T'CCnGG_A
Cuts at: 0 536 1515
Size: 536 979
PmlI CAC'GTG
Cuts at: 0 134 1515
Size: 134 1381
PpuMI rG'GwC_Cy
Cuts at: 0 264 863 1515
Size: 264 599 652
Fragments arranged by size:
652 599 264
PspOMI G'GGCC_C
Cuts at: 0 73 366 1515
Size: 73 293 1149
Fragments arranged by size:
1149 293 73
PvuII CAG'CTG
Cuts at: 0 113 831 1281 1515
Size: 113 718 450 234
Fragments arranged by size:
718 450 234 113
SacI G_AGCT'C
Cuts at: 0 259 1515
Size: 259 1256
SanDI GG'GwC_CC
Cuts at: 0 863 1515
Size: 863 652
ScaI AGT'ACT
Cuts at: 0 1154 1515
Size: 1154 361
SmaI CCC'GGG
Cuts at: 0 79 1515
Size: 79 1436
SmlI C'TyrA_G
Cuts at: 0 315 337 1515
Size: 315 22 1178
Fragments arranged by size:
1178 315 22
SspI AAT'ATT
Cuts at: 0 704 1515
Size: 704 811
StyI C'CwwG_G
Cuts at: 0 783 975 1515
Size: 783 192 540
Fragments arranged by size:
783 540 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 292 988 1515
Size: 292 696 527
Fragments arranged by size:
696 527 292
TatI w'GTAC_w
Cuts at: 0 1152 1515
Size: 1152 363
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 669 701 1171 1203 1515
Size: 669 32 470 32 312
Fragments arranged by size:
669 470 312 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 171 219 982 1056 1137 1515
Size: 171 48 763 74 81 378
Fragments arranged by size:
763 378 171 81 74 48
XmaI C'CCGG_G
Cuts at: 0 77 1515
Size: 77 1438
XmnI GAAnn'nnTTC
Cuts at: 0 241 703 1515
Size: 241 462 812
Fragments arranged by size:
812 462 241
Enzymes that do cut:
AarI AccI AcuI AflIII AgeI AhdI AleI AlwNI
ApaI ApaLI AseI AvaI BanI BanII BbsI BcgI
BclI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspHI BspMI BsrDI BsrFI BssSI BstAPI BstEII
BstYI Bsu36I BtgZI BtsI DraI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I FalI FspI FspAI
Hin4I MfeI MslI MspA1I NaeI NgoMIV NspI PflMI
PfoI PmlI PpuMI PspOMI PvuII SacI SanDI ScaI
SmaI SmlI SspI StyI TaqII TatI TstI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AlfI AloI ApoI
AscI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI
BfrBI BglI BglII BmtI BplI BsaI BsaBI BsaHI
BsiWI BsmI BsmBI BspEI BsrBI BsrGI BssHII BstBI
BstXI BstZ17I BtgI ClaI CspCI DrdI EcoNI EcoRI
EcoRV FseI HaeII HincII HindIII HpaI KasI KpnI
MluI MmeI MscI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PciI PmeI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacII SalI
SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI
XhoI ZraI