Restriction map for
pFN21AB4477
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# NO CUT ENZYMES ( 89 enzymes) #
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AccI AccII AccIII AclI AcsI
AflII AgeI ApoI AscI AseI
AsnI AspI AviII AvrII BamHI
BbrPI BbvCI BcgI BciVI BfrI
BlnI BsaAI BsaBI BseAI BsiWI
BsmI BspDI BspEI BspLU11I BssHII
Bst1107I BstBI BstUI BstZ17I ClaI
CpoI DraI DrdI EcoRI EcoRV
EcoT22I FseI FspI HpaI KspI
MamI MfeI MluI MroI MseI
MunI MvnI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
SfiI SfuI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tru9I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AatII 1318 GACGTC GACGT^C
Acc65I 437 GGTACC G^GTACC
AflIII 3248 ACACGT A^C[GA][CT]GT
Alw44I 2636 GTGCAC G^TGCAC
Aor51HI 2189 AGCGCT AGC^GCT
ApaI 2658 GGGCCC GGGCC^C
ApaLI 2636 GTGCAC G^TGCAC
Asp718I 437 GGTACC G^GTACC
BbeI 3130 GGCGCC GGCGC^C
BclI 1865 TGATCA T^GATCA
BsaI 2102 GGTCTC GGTCTC(1/5)
BsaWI 2099 TCCGGT [AT]^CCGG[AT]
BsiEI 2822 CGGCCG CG[GA][CT]^CG
BsmBI 612 CGTCTC CGTCTC(1/5)
Bsp1407I 1079 TGTACA T^GTACA
BsrGI 1079 TGTACA T^GTACA
BssSI 2859 CACGAG CACGAG(-5/-1)
BstAPI 2767 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EagI 2822 CGGCCG C^GGCCG
EclXI 2822 CGGCCG C^GGCCG
Eco47III 2189 AGCGCT AGC^GCT
Eco52I 2822 CGGCCG C^GGCCG
EcoNI 1389 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1865 TGATCA T^GATCA
HincII 412 GTCAAC GT[CT]^[GA]AC
HindII 412 GTCAAC GT[CT]^[GA]AC
HindIII 45 AAGCTT A^AGCTT
KasI 3130 GGCGCC G^GCGCC
KpnI 437 GGTACC GGTAC^C
NarI 3130 GGCGCC GG^CGCC
PflMI 1262 CCATCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ScaI 21 AGTACT AGT^ACT
SfoI 3130 GGCGCC GGC^GCC
SgrAI 2154 CACCGGCG C[GA]^CCGG[CT]G
SspBI 1079 TGTACA T^GTACA
Van91I 1262 CCATCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AhdI 2272 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3040 GACCAGCCGTC
AlwNI 175 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
2768 CAGCCTCTG
AspEI 2272 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3040 GACCAGCCGTC
BglI 1436 GCCCAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2824 GCCGGGGAGGC
BlpI 477 GCTGAGC GC^T[ACGT]AGC
2599 GCTGAGC
Bpu1102I 477 GCTGAGC GC^T[ACGT]AGC
2599 GCTGAGC
BspHI 923 TCATGA T^CATGA
1007 TCATGA
BsrBI 191 CCGCTC CCGCTC(-3/-3)
3115 GAGCGG
BstEII 2379 GGTGACC G^GT[ACGT]ACC
2520 GGTCACC
BstPI 2379 GGTGACC G^GT[ACGT]ACC
2520 GGTCACC
CelII 477 GCTGAGC GC^T[ACGT]AGC
2599 GCTGAGC
DraIII 1180 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2241 CACGGAGTG
Eam1105I 2272 GACTTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3040 GACCAGCCGTC
EcoO65I 2379 GGTGACC G^GT[ACGT]ACC
2520 GGTCACC
HgaI 2887 GACGC GACGC(5/10)
1598 GCGTC
NspI 1364 GCATGT [GA]CATG^[CT]
1477 GCATGT
PvuII 2015 CAGCTG CAG^CTG
2597 CAGCTG
RcaI 923 TCATGA T^CATGA
1007 TCATGA
SexAI 947 ACCTGGT A^CC[AT]GGT
2375 ACCAGGT
TaqI 94 TCGA T^CGA
1776 TCGA
TthHB8I 94 TCGA T^CGA
1776 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AocI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TfiI TseI Tsp45I
Tsp509I TspRI XcmI XhoII XmaCI
XmaI XmnI