(Circular) (Six-base) MAPSORT of: pFN21AB4477.seq Check: 1822 from: 1 to: 8152 pFN21AB4477 8152 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3327 5962 278 Size: 53 83 186 936 1791 2635 2468 Fragments arranged by size: 2635 2468 1791 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5495 1937 Size: 3558 4594 Acc65I G'GTAC_C Cuts at: 2442 5473 2442 Size: 3031 5121 AclI AA'CG_TT Cuts at: 1563 6279 6652 1563 Size: 4716 373 3063 Fragments arranged by size: 4716 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2132 5336 5386 6215 7599 7867 1632 Size: 500 3204 50 829 1384 268 1917 Fragments arranged by size: 3204 1917 1384 829 500 268 50 AfeI AGC'GCT Cuts at: 4196 4196 Size: 8152 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7955 AflIII A'CryG_T Cuts at: 1203 5253 7051 8028 1203 Size: 4050 1798 977 1327 Fragments arranged by size: 4050 1798 1327 977 AgeI A'CCGG_T Cuts at: 6983 6983 Size: 8152 AhdI GACnn_n'nnGTC Cuts at: 4282 5050 6881 4282 Size: 768 1831 5553 Fragments arranged by size: 5553 1831 768 AleI CACnn'nnGTG Cuts at: 1166 3698 4329 1166 Size: 2532 631 4989 Fragments arranged by size: 4989 2532 631 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8152 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2975 3007 2975 Size: 32 8120 AlwNI CAG_nnn'CTG Cuts at: 2185 4778 5895 7467 2185 Size: 2593 1117 1572 2870 Fragments arranged by size: 2870 2593 1572 1117 ApaI G_GGCC'C Cuts at: 4667 4667 Size: 8152 ApaLI G'TGCA_C Cuts at: 4641 6209 7365 4641 Size: 1568 1156 5428 Fragments arranged by size: 5428 1568 1156 ApoI r'AATT_y Cuts at: 1493 5461 5687 5839 7948 1493 Size: 3968 226 152 2109 1697 Fragments arranged by size: 3968 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6706 160 Size: 872 5674 1606 Fragments arranged by size: 5674 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8152 AvaI C'yCGr_G Cuts at: 1870 1943 2979 3284 3382 4272 4389 4477 4543 Size: 73 1036 305 98 890 117 88 66 Cuts at: 4543 4813 5085 5477 1870 Size: 270 272 392 4545 Fragments arranged by size: 4545 1036 890 392 305 272 270 117 98 88 73 66 BamHI G'GATC_C Cuts at: 5482 5849 5482 Size: 367 7785 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2442 4652 5135 5473 6928 Size: 325 217 627 655 2210 483 338 1455 Cuts at: 6928 618 Size: 1842 Fragments arranged by size: 2210 1842 1455 655 627 483 338 325 217 BanII G_rGCy'C Cuts at: 1391 2688 2988 4078 4097 4484 4667 4814 5471 Size: 1297 300 1090 19 387 183 147 657 Cuts at: 5471 1391 Size: 4072 Fragments arranged by size: 4072 1297 1090 657 387 300 183 147 19 BbeI G_GCGC'C Cuts at: 5139 5139 Size: 8152 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2879 3893 4300 4444 5046 928 Size: 971 980 1014 407 144 602 4034 Fragments arranged by size: 4034 1014 980 971 602 407 144 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6343 6377 1903 Size: 34 4406 34 3678 Fragments arranged by size: 4406 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6044 7254 6044 Size: 1210 6942 BclI T'GATC_A Cuts at: 1548 3870 1548 Size: 2322 5830 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3447 4835 6763 Size: 107 122 71 631 605 1775 1388 1928 Cuts at: 6763 136 Size: 1525 Fragments arranged by size: 1928 1775 1525 1388 631 605 122 107 71 BglII A'GATC_T Cuts at: 3099 3148 5355 8147 3099 Size: 49 2207 2792 3104 Fragments arranged by size: 3104 2792 2207 49 BlpI GC'TnA_GC Cuts at: 2483 4605 5569 2483 Size: 2122 964 5066 Fragments arranged by size: 5066 2122 964 Bme1580I G_kGCm'C Cuts at: 1790 3997 4326 4645 4655 4667 6213 7369 1790 Size: 2207 329 319 10 12 1546 1156 2573 Fragments arranged by size: 2573 2207 1546 1156 329 319 12 10 BmgBI CAC'GTC Cuts at: 1318 3700 1318 Size: 2382 5770 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2229 5883 6841 Size: 416 53 83 399 24 812 3654 958 Cuts at: 6841 442 Size: 1753 Fragments arranged by size: 3654 1753 958 812 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8152 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2452 3759 4940 2452 Size: 1307 1181 5664 Fragments arranged by size: 5664 1307 1181 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2447 3143 3273 3649 4171 4468 4812 5100 Size: 1208 696 130 376 522 297 344 288 Cuts at: 5100 5252 5351 6812 1239 Size: 152 99 1461 2579 Fragments arranged by size: 2579 1461 1208 696 522 376 344 297 288 152 130 99 Bpu10I CC'TnA_GC Cuts at: 3212 4096 4636 3212 Size: 884 540 6728 Fragments arranged by size: 6728 884 540 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6277 7142 7440 6277 Size: 865 298 6989 Fragments arranged by size: 6989 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4113 6815 882 Size: 617 2614 2702 2219 Fragments arranged by size: 2702 2614 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5943 493 Size: 711 4739 2702 Fragments arranged by size: 4739 2702 711 BsaBI GATnn'nnATC Cuts at: 8146 8146 Size: 8152 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3324 3603 5136 5959 Size: 53 83 186 936 1791 279 1533 823 Cuts at: 5959 6341 6976 275 Size: 382 635 1451 Fragments arranged by size: 1791 1533 1451 936 823 635 382 279 186 83 53 BsaWI w'CCGG_w Cuts at: 4104 6585 6983 7257 7404 7815 4104 Size: 2481 398 274 147 411 4441 Fragments arranged by size: 4441 2481 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2606 2636 2975 3005 3293 Size: 30 165 30 1203 30 339 30 288 Cuts at: 3293 3323 3497 3527 4241 4271 4358 4388 4685 Size: 30 174 30 714 30 87 30 297 Cuts at: 4685 4715 1178 Size: 30 4615 Fragments arranged by size: 4615 1203 714 339 297 288 174 165 87 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2611 4181 4184 4688 4693 4702 5402 1186 Size: 1425 1570 3 504 5 9 700 3936 Fragments arranged by size: 3936 1570 1425 700 504 9 5 3 BseYI C'CCAG_C Cuts at: 2388 2803 3589 4027 4046 4058 4904 4993 7355 Size: 415 786 438 19 12 846 89 2362 Cuts at: 7355 7851 2388 Size: 496 2689 Fragments arranged by size: 2689 2362 846 786 496 438 415 89 19 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2959 2978 3264 5465 1163 Size: 573 1223 19 286 2201 3850 Fragments arranged by size: 3850 2201 1223 573 286 19 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4830 5602 6363 7391 1540 Size: 275 186 2829 772 761 1028 2301 Fragments arranged by size: 2829 2301 1028 772 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3684 4645 4941 6213 6298 7369 3684 Size: 961 296 1272 85 1071 4467 Fragments arranged by size: 4467 1272 1071 961 296 85 BsmI GAATG_Cn' Cuts at: 5663 5756 5663 Size: 93 8059 BsmBI CGTCTCn'nnnn_ Cuts at: 2623 2623 Size: 8152 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2688 2988 3684 3997 4078 4097 4326 Size: 399 898 300 696 313 81 19 229 Cuts at: 4326 4484 4645 4655 4667 4814 4941 5471 6213 Size: 158 161 10 12 147 127 530 742 Cuts at: 6213 6298 7369 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 898 742 696 530 399 313 300 229 161 158 147 127 85 81 19 12 10 BspEI T'CCGG_A Cuts at: 7815 7815 Size: 8152 BspHI T'CATG_A Cuts at: 2928 3012 6041 7771 2928 Size: 84 3029 1730 3309 Fragments arranged by size: 3309 3029 1730 84 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3207 4513 4759 5507 844 Size: 2363 1306 246 748 3489 Fragments arranged by size: 3489 2363 1306 748 246 BsrBI CCG'CTC Cuts at: 1394 1823 2198 5122 6039 1394 Size: 429 375 2924 917 3507 Fragments arranged by size: 3507 2924 917 429 375 BsrDI GCAATG_nn' Cuts at: 65 1248 2128 2470 3770 4057 6647 6821 65 Size: 1183 880 342 1300 287 2590 174 1396 Fragments arranged by size: 2590 1396 1300 1183 880 342 287 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3054 3507 3959 4160 4339 5237 6796 Size: 110 1244 453 452 201 179 898 1559 Cuts at: 6796 6983 1700 Size: 187 2869 Fragments arranged by size: 2869 1559 1244 898 453 452 201 187 179 110 BsrGI T'GTAC_A Cuts at: 96 3084 96 Size: 2988 5164 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8152 BssSI C'ACGA_G Cuts at: 4864 6212 7224 4864 Size: 1348 1012 5792 Fragments arranged by size: 5792 1348 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4778 794 Size: 3984 4168 BstBI TT'CG_AA Cuts at: 6956 6956 Size: 8152 BstEII G'GTnAC_C Cuts at: 1184 4384 4525 1184 Size: 3200 141 4811 Fragments arranged by size: 4811 3200 141 BstXI CCAn_nnnn'nTGG Cuts at: 2899 4870 5152 2899 Size: 1971 282 5899 Fragments arranged by size: 5899 1971 282 BstYI r'GATC_y Cuts at: 1972 3099 3148 5355 5482 5849 6234 6251 7703 Size: 1127 49 2207 127 367 385 17 1452 Cuts at: 7703 7789 7801 8147 1972 Size: 86 12 346 1977 Fragments arranged by size: 2207 1977 1452 1127 385 367 346 127 86 49 17 12 Bsu36I CC'TnA_GG Cuts at: 2178 3041 4585 2178 Size: 863 1544 5745 Fragments arranged by size: 5745 1544 863 BtgI C'CryG_G Cuts at: 513 1065 2004 2446 2892 3072 3111 3607 4231 Size: 552 939 442 446 180 39 496 624 Cuts at: 4231 4287 4530 513 Size: 56 243 4135 Fragments arranged by size: 4135 939 624 552 496 446 442 243 180 56 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7413 Fragments arranged by size: 7413 683 56 BtsI GCAGTG_nn' Cuts at: 2116 2592 3497 4585 5232 5383 5668 6462 6482 Size: 476 905 1088 647 151 285 794 20 Cuts at: 6482 2116 Size: 3786 Fragments arranged by size: 3786 1088 905 794 647 476 285 151 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8152 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8117 DraI TTT'AAA Cuts at: 5456 5456 Size: 8152 DraIII CAC_nnn'GTG Cuts at: 3190 4251 3190 Size: 1061 7091 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7159 809 Size: 507 5843 1802 Fragments arranged by size: 5843 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2153 2280 2380 Size: 54 1425 50 275 18 323 127 100 Cuts at: 2380 2536 2646 2676 2865 3090 3937 4827 4944 Size: 156 110 30 189 225 847 890 117 Cuts at: 4944 5211 5388 5599 6488 8117 8 Size: 267 177 211 889 1629 43 Fragments arranged by size: 1629 1425 890 889 847 323 275 267 225 211 189 177 156 127 117 110 100 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 4827 5599 1537 Size: 275 3015 772 4090 Fragments arranged by size: 4090 3015 772 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2898 3963 4349 5100 6082 1353 Size: 1545 1065 386 751 982 3423 Fragments arranged by size: 3423 1545 1065 982 751 386 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4204 4954 6739 7113 7259 387 Size: 1064 138 2615 750 1785 374 146 1280 Fragments arranged by size: 2615 1785 1280 1064 750 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2132 2447 3143 3273 3649 4171 4468 Size: 393 500 315 696 130 376 522 297 Cuts at: 4468 4812 5100 5252 5336 5351 5386 6215 6812 Size: 344 288 152 84 15 35 829 597 Cuts at: 6812 7599 7867 1239 Size: 787 268 1524 Fragments arranged by size: 1524 829 787 696 597 522 500 393 376 344 315 297 288 268 152 130 84 35 15 EcoNI CCTnn'n_nnAGG Cuts at: 3398 3398 Size: 8152 EcoO109I rG'GnC_Cy Cuts at: 2392 3634 3660 3794 4063 4590 4663 4664 4921 Size: 1242 26 134 269 527 73 1 257 Cuts at: 4921 4980 2392 Size: 59 5564 Fragments arranged by size: 5564 1242 527 269 257 134 73 59 26 1 EcoRI G'AATT_C Cuts at: 5461 5839 5461 Size: 378 7774 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3151 3871 5323 3151 Size: 720 1452 5980 Fragments arranged by size: 5980 1452 720 FspI TGC'GCA Cuts at: 6658 6658 Size: 8152 HaeII r_GCGC'y Cuts at: 1678 3442 3829 3867 4198 5139 5177 7299 1678 Size: 1764 387 38 331 941 38 2122 2531 Fragments arranged by size: 2531 2122 1764 941 387 331 38 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3497 3529 4241 4268 4273 Size: 32 135 32 1925 32 712 27 5 Cuts at: 4273 4300 4358 4390 5036 5068 5304 5336 6793 Size: 27 58 32 646 32 236 32 1457 Cuts at: 6793 6825 6867 6899 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1925 1457 712 646 236 135 58 42 32 32 32 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 2419 5496 5742 669 Size: 988 281 481 3077 246 3079 Fragments arranged by size: 3079 3077 988 481 281 246 HindIII A'AGCT_T Cuts at: 748 2050 748 Size: 1302 6850 HpaI GTT'AAC Cuts at: 5742 5742 Size: 8152 KasI G'GCGC_C Cuts at: 5135 5135 Size: 8152 KpnI G_GTAC'C Cuts at: 2446 5477 2446 Size: 3031 5121 MfeI C'AATT_G Cuts at: 5751 5751 Size: 8152 MluI A'CGCG_T Cuts at: 8028 8028 Size: 8152 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2183 3345 7266 7450 1305 Size: 878 1162 3921 184 2007 Fragments arranged by size: 3921 2007 1162 878 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2282 2382 2538 3092 5213 Size: 54 1425 343 450 100 156 554 2121 Cuts at: 5213 10 Size: 2949 Fragments arranged by size: 2949 2121 1425 554 450 343 156 100 54 MslI CAynn'nnrTG Cuts at: 518 1166 2121 2789 2897 3698 4329 6110 6469 Size: 648 955 668 108 801 631 1781 359 Cuts at: 6469 6628 518 Size: 159 2042 Fragments arranged by size: 2042 1781 955 801 668 648 631 359 159 108 MspA1I CmG'CkG Cuts at: 1606 2152 2471 2490 4022 4604 5133 5387 5568 Size: 546 319 19 1532 582 529 254 181 Cuts at: 5568 6245 7393 7638 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1532 1148 677 582 546 529 319 254 245 181 19 NaeI GCC'GGC Cuts at: 3961 4341 5239 3961 Size: 380 898 6874 Fragments arranged by size: 6874 898 380 NarI GG'CG_CC Cuts at: 5136 5136 Size: 8152 NcoI C'CATG_G Cuts at: 513 1065 2004 2446 2892 3111 4231 513 Size: 552 939 442 446 219 1120 4434 Fragments arranged by size: 4434 1120 939 552 446 442 219 NdeI CA'TA_TG Cuts at: 387 387 Size: 8152 NgoMIV G'CCGG_C Cuts at: 3959 4339 5237 3959 Size: 380 898 6874 Fragments arranged by size: 6874 898 380 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8152 NotI GC'GGCC_GC Cuts at: 5599 5599 Size: 8152 NspI r_CATG'y Cuts at: 1132 1229 3373 3486 5510 7055 1132 Size: 97 2144 113 2024 1545 2229 Fragments arranged by size: 2229 2144 2024 1545 113 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8152 PciI A'CATG_T Cuts at: 7051 7051 Size: 8152 PflMI CCAn_nnn'nTGG Cuts at: 1108 3273 1108 Size: 2165 5987 PfoI T'CCnGG_A Cuts at: 3030 3552 3665 3924 4080 5157 5343 3030 Size: 522 113 259 156 1077 186 5839 Fragments arranged by size: 5839 1077 522 259 186 156 113 PmeI GTTT'AAAC Cuts at: 5456 5456 Size: 8152 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2975 3007 6221 6253 7734 7766 2975 Size: 32 3214 32 1481 32 3361 Fragments arranged by size: 3361 3214 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2392 3660 3794 4063 4590 4980 2392 Size: 1268 134 269 527 390 5564 Fragments arranged by size: 5564 1268 527 390 269 134 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8152 PsiI TTA'TAA Cuts at: 5722 5722 Size: 8152 PspOMI G'GGCC_C Cuts at: 4663 4663 Size: 8152 PstI C_TGCA'G Cuts at: 830 2926 3633 3679 4603 4783 5504 830 Size: 2096 707 46 924 180 721 3478 Fragments arranged by size: 3478 2096 924 721 707 180 46 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8152 PvuII CAG'CTG Cuts at: 4022 4604 4022 Size: 582 7570 SalI G'TCGA_C Cuts at: 1936 5494 1936 Size: 3558 4594 SanDI GG'GwC_CC Cuts at: 2392 4063 4590 4980 2392 Size: 1671 527 390 5564 Fragments arranged by size: 5564 1671 527 390 SbfI CC_TGCA'GG Cuts at: 5504 5504 Size: 8152 ScaI AGT'ACT Cuts at: 2028 6400 8112 2028 Size: 4372 1712 2068 Fragments arranged by size: 4372 2068 1712 SexAI A'CCwGG_T Cuts at: 2952 4380 2952 Size: 1428 6724 SfcI C'TryA_G Cuts at: 826 1045 2922 3629 3675 4599 4779 5500 6635 Size: 219 1877 707 46 924 180 721 1135 Cuts at: 6635 7316 7507 826 Size: 681 191 1471 Fragments arranged by size: 1877 1471 1135 924 721 707 681 219 191 180 46 SfoI GGC'GCC Cuts at: 5137 5137 Size: 8152 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8152 SgrAI Cr'CCGG_yG Cuts at: 1700 4160 1700 Size: 2460 5692 SmaI CCC'GGG Cuts at: 1872 2981 3384 4479 5479 1872 Size: 1109 403 1095 1000 4545 Fragments arranged by size: 4545 1109 1095 1000 403 SmlI C'TyrA_G Cuts at: 820 1017 1943 6256 7157 7419 820 Size: 197 926 4313 901 262 1553 Fragments arranged by size: 4313 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5943 493 Size: 5450 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8152 SphI G_CATG'C Cuts at: 1132 5510 1132 Size: 4378 3774 SrfI GCCC'GGGC Cuts at: 4479 4479 Size: 8152 SspI AAT'ATT Cuts at: 5 52 6076 5 Size: 47 6024 2081 Fragments arranged by size: 6024 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8152 StyI C'CwwG_G Cuts at: 513 1065 2004 2211 2383 2446 2892 2965 3111 Size: 552 939 207 172 63 446 73 146 Cuts at: 3111 3720 4011 4231 4239 5145 5591 513 Size: 609 291 220 8 906 446 3074 Fragments arranged by size: 3074 939 906 609 552 446 446 291 220 207 172 146 73 63 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6179 6196 6349 6534 6979 325 Size: 1255 4599 17 153 185 445 1498 Fragments arranged by size: 4599 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2026 3084 6398 Size: 274 80 33 51 1443 49 1058 3314 Cuts at: 6398 8110 96 Size: 1712 138 Fragments arranged by size: 3314 1712 1443 1058 274 138 80 51 49 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4235 4267 4685 4717 8112 8144 4235 Size: 32 418 32 3395 32 4243 Fragments arranged by size: 4243 3395 418 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8152 XbaI T'CTAG_A Cuts at: 5488 5488 Size: 8152 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2375 3118 5326 2375 Size: 743 2208 5201 Fragments arranged by size: 5201 2208 743 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8152 XmaI C'CCGG_G Cuts at: 1870 2979 3382 4477 5477 1870 Size: 1109 403 1095 1000 4545 Fragments arranged by size: 4545 1109 1095 1000 403 XmnI GAAnn'nnTTC Cuts at: 2145 2220 3134 6281 2145 Size: 75 914 3147 4016 Fragments arranged by size: 4016 3147 914 75 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3325 5960 276 Size: 53 83 186 936 1791 2635 2468 Fragments arranged by size: 2635 2468 1791 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SapI SfiI SwaI