Restriction map for pFN21AB4477
 (Linear) (Six-base) MAPSORT of: KIBB4477.seq  Check: 9982  from: 1  to: 3447



KIBB4477.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:26  ..

AatII G_ACGT'C

Cuts at:      0    1322    3447 
   Size:       1322    2125
 
Acc65I G'GTAC_C

Cuts at:      0     437    3447 
   Size:        437    3010
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127    3331    3381    3447 
   Size:        127    3204      50      66

  Fragments arranged by size: 

                3204     127      66      50
 
AfeI AGC'GCT

Cuts at:      0    2191    3447 
   Size:       2191    1256
 
AflIII A'CryG_T

Cuts at:      0    3248    3447 
   Size:       3248     199
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2277    3045    3447 
   Size:       2277     768     402

  Fragments arranged by size: 

                2277     768     402
 
AleI CACnn'nnGTG

Cuts at:      0    1693    2324    3447 
   Size:       1693     631    1123

  Fragments arranged by size: 

                1693    1123     631
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     970    1002    3447 
   Size:        970      32    2445

  Fragments arranged by size: 

                2445     970      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     180    2773    3447 
   Size:        180    2593     674

  Fragments arranged by size: 

                2593     674     180
 
ApaI G_GGCC'C

Cuts at:      0    2662    3447 
   Size:       2662     785
 
ApaLI G'TGCA_C

Cuts at:      0    2636    3447 
   Size:       2636     811
 
AvaI C'yCGr_G

Cuts at:      0     974    1279    1377    2267    2384    2472    2538    2808 
   Size:        974     305      98     890     117      88      66     270

Cuts at:   2808    3080    3447 
   Size:        272     367

  Fragments arranged by size: 

                 974     890     367     305     272     270     117      98
                  88      66
 
BanI G'GyrC_C

Cuts at:      0     437    2647    3130    3447 
   Size:        437    2210     483     317

  Fragments arranged by size: 

                2210     483     437     317
 
BanII G_rGCy'C

Cuts at:      0     683     983    2073    2092    2479    2662    2809    3447 
   Size:        683     300    1090      19     387     183     147     638

  Fragments arranged by size: 

                1090     683     638     387     300     183     147      19
            
 
BbeI G_GCGC'C

Cuts at:      0    3134    3447 
   Size:       3134     313
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     874    1888    2295    2439    3041    3447 
   Size:        874    1014     407     144     602     406

  Fragments arranged by size: 

                1014     874     602     407     406     144
 
BclI T'GATC_A

Cuts at:      0    1865    3447 
   Size:       1865    1582
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1442    2830    3447 
   Size:       1442    1388     617

  Fragments arranged by size: 

                1442    1388     617
 
BglII A'GATC_T

Cuts at:      0    1094    1143    3350    3447 
   Size:       1094      49    2207      97

  Fragments arranged by size: 

                2207    1094      97      49
 
BlpI GC'TnA_GC

Cuts at:      0     478    2600    3447 
   Size:        478    2122     847

  Fragments arranged by size: 

                2122     847     478
 
Bme1580I G_kGCm'C

Cuts at:      0    1992    2321    2640    2650    2662    3447 
   Size:       1992     329     319      10      12     785

  Fragments arranged by size: 

                1992     785     329     319      12      10
 
BmgBI CAC'GTC

Cuts at:      0    1695    3447 
   Size:       1695    1752
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     224    3447 
   Size:        224    3223
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     447    1754    2935    3447 
   Size:        447    1307    1181     512

  Fragments arranged by size: 

                1307    1181     512     447
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     442    1138    1268    1644    2166    2463    2807    3095 
   Size:        442     696     130     376     522     297     344     288

Cuts at:   3095    3247    3346    3447 
   Size:        152      99     101

  Fragments arranged by size: 

                 696     522     442     376     344     297     288     152
                 130     101      99
 
Bpu10I CC'TnA_GC

Cuts at:      0    1207    2091    2631    3447 
   Size:       1207     884     540     816

  Fragments arranged by size: 

                1207     884     816     540
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2108    3447 
   Size:       2108    1339
 
BsaHI Gr'CG_yC

Cuts at:      0    1319    1598    3131    3447 
   Size:       1319     279    1533     316

  Fragments arranged by size: 

                1533    1319     316     279
 
BsaWI w'CCGG_w

Cuts at:      0    2099    3447 
   Size:       2099    1348
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     601     631     970    1000    1288    1318    1492    1522 
   Size:        601      30     339      30     288      30     174      30

Cuts at:   1522    2236    2266    2353    2383    2680    2710    3447 
   Size:        714      30      87      30     297      30     737

  Fragments arranged by size: 

                 737     714     601     339     297     288     174      87
                  30      30      30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     606    2176    2179    2683    2688    2697    3397    3447 
   Size:        606    1570       3     504       5       9     700      50

  Fragments arranged by size: 

                1570     700     606     504      50       9       5       3
            
 
BseYI C'CCAG_C

Cuts at:      0     383     798    1584    2022    2041    2053    2899    2988 
   Size:        383     415     786     438      19      12     846      89

Cuts at:   2988    3447 
   Size:        459

  Fragments arranged by size: 

                 846     786     459     438     415     383      89      19
                  12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     954     973    1259    3447 
   Size:        954      19     286    2188

  Fragments arranged by size: 

                2188     954     286      19
 
BsiEI CG_ry'CG

Cuts at:      0    2825    3447 
   Size:       2825     622
 
BsiHKAI G_wGCw'C

Cuts at:      0    1679    2640    2936    3447 
   Size:       1679     961     296     511

  Fragments arranged by size: 

                1679     961     511     296
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     618    3447 
   Size:        618    2829
 
Bsp1286I G_dGCh'C

Cuts at:      0     683     983    1679    1992    2073    2092    2321    2479 
   Size:        683     300     696     313      81      19     229     158

Cuts at:   2479    2640    2650    2662    2809    2936    3447 
   Size:        161      10      12     147     127     511

  Fragments arranged by size: 

                 696     683     511     313     300     229     161     158
                 147     127      81      19      12      10
 
BspHI T'CATG_A

Cuts at:      0     923    1007    3447 
   Size:        923      84    2440

  Fragments arranged by size: 

                2440     923      84
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1202    2508    2754    3447 
   Size:       1202    1306     246     693

  Fragments arranged by size: 

                1306    1202     693     246
 
BsrBI CCG'CTC

Cuts at:      0     193    3117    3447 
   Size:        193    2924     330

  Fragments arranged by size: 

                2924     330     193
 
BsrDI GCAATG_nn'

Cuts at:      0     123     465    1765    2052    3447 
   Size:        123     342    1300     287    1395

  Fragments arranged by size: 

                1395    1300     342     287     123
 
BsrFI r'CCGG_y

Cuts at:      0    1049    1502    1954    2155    2334    3232    3447 
   Size:       1049     453     452     201     179     898     215

  Fragments arranged by size: 

                1049     898     453     452     215     201     179
 
BsrGI T'GTAC_A

Cuts at:      0    1079    3447 
   Size:       1079    2368
 
BssSI C'ACGA_G

Cuts at:      0    2859    3447 
   Size:       2859     588
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2773    3447 
   Size:       2773     674
 
BstEII G'GTnAC_C

Cuts at:      0    2379    2520    3447 
   Size:       2379     141     927

  Fragments arranged by size: 

                2379     927     141
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     894    2865    3147    3447 
   Size:        894    1971     282     300

  Fragments arranged by size: 

                1971     894     300     282
 
BstYI r'GATC_y

Cuts at:      0    1094    1143    3350    3447 
   Size:       1094      49    2207      97

  Fragments arranged by size: 

                2207    1094      97      49
 
Bsu36I CC'TnA_GG

Cuts at:      0     173    1036    2580    3447 
   Size:        173     863    1544     867

  Fragments arranged by size: 

                1544     867     863     173
 
BtgI C'CryG_G

Cuts at:      0     441     887    1067    1106    1602    2226    2282    2525 
   Size:        441     446     180      39     496     624      56     243

Cuts at:   2525    3447 
   Size:        922

  Fragments arranged by size: 

                 922     624     496     446     441     243     180      56
                  39
 
BtsI GCAGTG_nn'

Cuts at:      0     111     587    1492    2580    3227    3378    3447 
   Size:        111     476     905    1088     647     151      69

  Fragments arranged by size: 

                1088     905     647     476     151     111      69
 
DraIII CAC_nnn'GTG

Cuts at:      0    1185    2246    3447 
   Size:       1185    1061    1201

  Fragments arranged by size: 

                1201    1185    1061
 
EaeI y'GGCC_r

Cuts at:      0     148     275     375     531     641     671     860    1085 
   Size:        148     127     100     156     110      30     189     225

Cuts at:   1085    1932    2822    2939    3206    3383    3447 
   Size:        847     890     117     267     177      64

  Fragments arranged by size: 

                 890     847     267     225     189     177     156     148
                 127     117     110     100      64      30
 
EagI C'GGCC_G

Cuts at:      0    2822    3447 
   Size:       2822     625
 
EarI CTCTTCn'nnn_

Cuts at:      0     893    1958    2344    3095    3447 
   Size:        893    1065     386     751     352

  Fragments arranged by size: 

                1065     893     751     386     352
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2199    2949    3447 
   Size:       2199     750     498

  Fragments arranged by size: 

                2199     750     498
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     442    1138    1268    1644    2166    2463    2807 
   Size:        127     315     696     130     376     522     297     344

Cuts at:   2807    3095    3247    3331    3346    3381    3447 
   Size:        288     152      84      15      35      66

  Fragments arranged by size: 

                 696     522     376     344     315     297     288     152
                 130     127      84      66      35      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1393    3447 
   Size:       1393    2054
 
EcoO109I rG'GnC_Cy

Cuts at:      0     387    1629    1655    1789    2058    2585    2658    2659 
   Size:        387    1242      26     134     269     527      73       1

Cuts at:   2659    2916    2975    3447 
   Size:        257      59     472

  Fragments arranged by size: 

                1242     527     472     387     269     257     134      73
                  59      26       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1146    1866    3318    3447 
   Size:       1146     720    1452     129

  Fragments arranged by size: 

                1452    1146     720     129
 
HaeII r_GCGC'y

Cuts at:      0    1437    1824    1862    2193    3134    3172    3447 
   Size:       1437     387      38     331     941      38     275

  Fragments arranged by size: 

                1437     941     387     331     275      38      38
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1492    1524    2236    2263    2268    2295    2353    2385 
   Size:       1492      32     712      27       5      27      58      32

Cuts at:   2385    3031    3063    3299    3331    3447 
   Size:        646      32     236      32     116

  Fragments arranged by size: 

                1492     712     646     236     116      58      32      32
                  32      32      27      27       5
 
HincII GTy'rAC

Cuts at:      0     414    3447 
   Size:        414    3033
 
HindIII A'AGCT_T

Cuts at:      0      45    3447 
   Size:         45    3402
 
KasI G'GCGC_C

Cuts at:      0    3130    3447 
   Size:       3130     317
 
KpnI G_GTAC'C

Cuts at:      0     441    3447 
   Size:        441    3006
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     178    1340    3447 
   Size:        178    1162    2107

  Fragments arranged by size: 

                2107    1162     178
 
MscI TGG'CCA

Cuts at:      0     277     377     533    1087    3208    3447 
   Size:        277     100     156     554    2121     239

  Fragments arranged by size: 

                2121     554     277     239     156     100
 
MslI CAynn'nnrTG

Cuts at:      0     116     784     892    1693    2324    3447 
   Size:        116     668     108     801     631    1123

  Fragments arranged by size: 

                1123     801     668     631     116     108
 
MspA1I CmG'CkG

Cuts at:      0     147     466     485    2017    2599    3128    3382    3447 
   Size:        147     319      19    1532     582     529     254      65

  Fragments arranged by size: 

                1532     582     529     319     254     147      65      19
            
 
NaeI GCC'GGC

Cuts at:      0    1956    2336    3234    3447 
   Size:       1956     380     898     213

  Fragments arranged by size: 

                1956     898     380     213
 
NarI GG'CG_CC

Cuts at:      0    3131    3447 
   Size:       3131     316
 
NcoI C'CATG_G

Cuts at:      0     441     887    1106    2226    3447 
   Size:        441     446     219    1120    1221

  Fragments arranged by size: 

                1221    1120     446     441     219
 
NgoMIV G'CCGG_C

Cuts at:      0    1954    2334    3232    3447 
   Size:       1954     380     898     215

  Fragments arranged by size: 

                1954     898     380     215
 
NspI r_CATG'y

Cuts at:      0    1368    1481    3447 
   Size:       1368     113    1966

  Fragments arranged by size: 

                1966    1368     113
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1268    3447 
   Size:       1268    2179
 
PfoI T'CCnGG_A

Cuts at:      0    1025    1547    1660    1919    2075    3152    3338    3447 
   Size:       1025     522     113     259     156    1077     186     109

  Fragments arranged by size: 

                1077    1025     522     259     186     156     113     109
            
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     970    1002    3447 
   Size:        970      32    2445

  Fragments arranged by size: 

                2445     970      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     387    1655    1789    2058    2585    2975    3447 
   Size:        387    1268     134     269     527     390     472

  Fragments arranged by size: 

                1268     527     472     390     387     269     134
 
PspOMI G'GGCC_C

Cuts at:      0    2658    3447 
   Size:       2658     789
 
PstI C_TGCA'G

Cuts at:      0     921    1628    1674    2598    2778    3447 
   Size:        921     707      46     924     180     669

  Fragments arranged by size: 

                 924     921     707     669     180      46
 
PvuII CAG'CTG

Cuts at:      0    2017    2599    3447 
   Size:       2017     582     848

  Fragments arranged by size: 

                2017     848     582
 
SanDI GG'GwC_CC

Cuts at:      0     387    2058    2585    2975    3447 
   Size:        387    1671     527     390     472

  Fragments arranged by size: 

                1671     527     472     390     387
 
ScaI AGT'ACT

Cuts at:      0      23    3447 
   Size:         23    3424
 
SexAI A'CCwGG_T

Cuts at:      0     947    2375    3447 
   Size:        947    1428    1072

  Fragments arranged by size: 

                1428    1072     947
 
SfcI C'TryA_G

Cuts at:      0     917    1624    1670    2594    2774    3447 
   Size:        917     707      46     924     180     673

  Fragments arranged by size: 

                 924     917     707     673     180      46
 
SfoI GGC'GCC

Cuts at:      0    3132    3447 
   Size:       3132     315
 
SgrAI Cr'CCGG_yG

Cuts at:      0    2155    3447 
   Size:       2155    1292
 
SmaI CCC'GGG

Cuts at:      0     976    1379    2474    3447 
   Size:        976     403    1095     973

  Fragments arranged by size: 

                1095     976     973     403
 
SrfI GCCC'GGGC

Cuts at:      0    2474    3447 
   Size:       2474     973
 
StyI C'CwwG_G

Cuts at:      0     206     378     441     887     960    1106    1715    2006 
   Size:        206     172      63     446      73     146     609     291

Cuts at:   2006    2226    2234    3140    3447 
   Size:        220       8     906     307

  Fragments arranged by size: 

                 906     609     446     307     291     220     206     172
                 146      73      63       8
 
TatI w'GTAC_w

Cuts at:      0      21    1079    3447 
   Size:         21    1058    2368

  Fragments arranged by size: 

                2368    1058      21
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2230    2262    2680    2712    3447 
   Size:       2230      32     418      32     735

  Fragments arranged by size: 

                2230     735     418      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     370    1113    3321    3447 
   Size:        370     743    2208     126

  Fragments arranged by size: 

                2208     743     370     126
 
XmaI C'CCGG_G

Cuts at:      0     974    1377    2472    3447 
   Size:        974     403    1095     975

  Fragments arranged by size: 

                1095     975     974     403
 
XmnI GAAnn'nnTTC

Cuts at:      0     140     215    1129    3447 
   Size:        140      75     914    2318

  Fragments arranged by size: 

                2318     914     140      75
 
ZraI GAC'GTC

Cuts at:      0    1320    3447 
   Size:       1320    2127
 

 Enzymes that do cut:

    AatII   Acc65I     AcuI     AfeI   AflIII     AhdI     AleI     AloI
    AlwNI     ApaI    ApaLI     AvaI     BanI    BanII     BbeI     BbsI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtgI     BtsI   DraIII     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I     FalI    HaeII    Hin4I   HincII  HindIII
     KasI     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI    PflMI     PfoI     PpiI    PpuMI   PspOMI
     PstI    PvuII    SanDI     ScaI    SexAI     SfcI     SfoI    SgrAI
     SmaI     SrfI     StyI     TatI     TstI     XcmI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AccI     AclI    AflII     AgeI     AlfI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI    BciVI
    BfrBI     BmtI    BpuEI    BsaAI    BsaBI    BsiWI     BsmI    BspEI
   BssHII    BstBI  BstZ17I    BtgZI     ClaI    CspCI     DraI     DrdI
  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI     HpaI     MfeI
     MluI     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI     SapI     SbfI     SfiI     SgfI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     SwaI    TaqII
  Tth111I     XbaI     XhoI
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