Restriction map for
pFN21AB4421
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BbeI BbrPI BcgI
BciVI BclI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MamI MluI MroI MspA1I NaeI
NarI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
Psp1406I PstI PvuI PvuII RsrII
SacI SacII SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 1174 GTCGAC GT^[AC][GT]AC
AccII 111 CGCG CG^CG
AflIII 1159 ACACGT A^C[GA][CT]GT
AlwI 638 GGATC GGATC(4/5)
AseI 843 ATTAAT AT^TAAT
AsnI 843 ATTAAT AT^TAAT
BalI 804 TGGCCA TGG^CCA
BanI 1479 GGCACC G^G[CT][GA]CC
BbsI 574 GAAGAC GAAGAC(2/6)
BbvCI 61 GCTGAGG CCTCAGC(-5/-2)
BcnI 39 CCCGG CC^[GC]GG
BfaI 230 CTAG C^TAG
BglII 963 AGATCT A^GATCT
BpuAI 574 GAAGAC GAAGAC(2/6)
BsaI 650 GAGACC GGTCTC(1/5)
BspHI 732 TCATGA T^CATGA
BspMI 1559 ACCTGC ACCTGC(4/8)
BsrDI 382 GCAATG GCAATG(2/0)
BstUI 111 CGCG CG^CG
BstXI 608 CCATGGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 963 AGATCT [GA]^GATC[CT]
DraI 1105 TTTAAA TTT^AAA
HincII 1174 GTCGAC GT[CT]^[GA]AC
HindII 1174 GTCGAC GT[CT]^[GA]AC
HindIII 1526 AAGCTT A^AGCTT
MaeI 230 CTAG C^TAG
MaeII 1161 ACGT A^CGT
MfeI 902 CAATTG C^AATTG
MflI 963 AGATCT [GA]^GATC[CT]
MluNI 804 TGGCCA TGG^CCA
MscI 804 TGGCCA TGG^CCA
MunI 902 CAATTG C^AATTG
MvnI 111 CGCG CG^CG
NciI 39 CCCGG CC^[GC]GG
NspI 854 ACATGC [GA]CATG^[CT]
PflMI 874 CCATTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1189 GACTC GAGTC(4/5)
PshAI 1167 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 843 ATTAAT AT^TAAT
RcaI 732 TCATGA T^CATGA
SalI 1174 GTCGAC G^TCGAC
SfcI 645 CTACAG C^T[GA][CT]AG
SmlI 514 CTTGAG C^T[CT][GA]AG
SpeI 229 ACTAGT A^CTAGT
Van91I 874 CCATTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 165 CCATGGGGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 963 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
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AciI 18 GCGG CCGC(-3/-1)
112 GCGG
AcsI 211 AAATTT [GA]^AATT[CT]
1348 AAATTT
ApoI 211 AAATTT [GA]^AATT[CT]
1348 AAATTT
Asp700I 724 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1072 GAAGAACTTC
AspHI 866 GAGCAC G[AT]GC[AT]^C
1555 GAGCAC
BanII 114 GGGCTC G[GA]GC[CT]^C
1096 GAGCCC
BseRI 133 GAGGAG GAGGAG(10/8)
530 GAGGAG
BsiHKAI 866 GAGCAC G[AT]GC[AT]^C
1555 GAGCAC
BsmAI 50 GAGAC GTCTC(1/5)
650 GAGAC
BsmFI 20 GGGAC GGGAC(10/14)
1022 GGGAC
DsaI 165 CCATGG C^C[GA][CT]GG
608 CCATGG
EarI 1126 GAAGAG CTCTTC(1/4)
1373 GAAGAG
FauI 18 GCGGG CCCGC(4/6)
112 GCGGG
HapII 40 CCGG C^CGG
661 CCGG
HgaI 109 GACGC GACGC(5/10)
1024 GACGC
HpaII 40 CCGG C^CGG
661 CCGG
HphI 681 TCACC GGTGA(8/7)
1300 TCACC
Ksp632I 1126 GAAGAG CTCTTC(1/4)
1373 GAAGAG
MslI 363 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
808 CACAATCGTG
MspI 40 CCGG C^CGG
661 CCGG
MwoI 969 GCTTAAAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1387 GCCAGCACAGC
NcoI 165 CCATGG C^CATGG
608 CCATGG
NdeI 182 CATATG CA^TATG
1235 CATATG
SfaNI 453 GCATC GCATC(5/9)
1474 GCATC
Tsp45I 745 GTCAC ^GT[GC]AC
1456 GTGAC
XmnI 724 GAATCTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1072 GAAGAACTTC
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AfaI AluI AvaII BbvI BmyI
BpmI BsaJI BsiYI BslI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII EaeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
ItaI MaeIII MboI MboII MnlI
MseI MvaI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SspI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I