Restriction map for pFN21AB4421
 (Circular) (Six-base) MAPSORT of: pFN21AB4421.seq  Check: 4933  from: 1  to: 6280



pFN21AB4421  6280 bp

 With 182 enzymes: SgfI * 

                        February 27, 2009 14:33  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   3573    3573 
   Size:       6280
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    4090     278 
   Size:         53      83     186     936    2554    2468

  Fragments arranged by size: 

                2554    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3180    3623    1937 
   Size:       1243     443    4594

  Fragments arranged by size: 

                4594    1243     443
 
Acc65I G'GTAC_C

Cuts at:   3601    3601 
   Size:       6280
 
AclI AA'CG_TT

Cuts at:   1563    4407    4780    1563 
   Size:       2844     373    3063

  Fragments arranged by size: 

                3063    2844     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2681    3150    4343    5727    5995    1632 
   Size:       1049     469    1193    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1193    1049     469     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    6083
 
AflIII A'CryG_T

Cuts at:   1203    3164    5179    6156    1203 
   Size:       1961    2015     977    1327

  Fragments arranged by size: 

                2015    1961    1327     977
 
AgeI A'CCGG_T

Cuts at:   5111    5111 
   Size:       6280
 
AhdI GACnn_n'nnGTC

Cuts at:   5009    5009 
   Size:       6280
 
AleI CACnn'nnGTG

Cuts at:   1166    2817    1166 
   Size:       1651    4629
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       6280
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3282    3314    3282 
   Size:         32    6248
 
AlwNI CAG_nnn'CTG

Cuts at:   4023    5595    4023 
   Size:       1572    4708
 
ApaLI G'TGCA_C

Cuts at:   4337    5493    4337 
   Size:       1156    5124
 
ApoI r'AATT_y

Cuts at:   1493    2216    3353    3589    3815    3967    6076    1493 
   Size:        723    1137     236     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1137     723     236     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    2849    4834     160 
   Size:        872    1817    1985    1606

  Fragments arranged by size: 

                1985    1817    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6280
 
AvaI C'yCGr_G

Cuts at:   1870    1943    3605    1870 
   Size:         73    1662    4545

  Fragments arranged by size: 

                4545    1662      73
 
BamHI G'GATC_C

Cuts at:   3610    3977    3610 
   Size:        367    5913
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3484    3601    5056     618 
   Size:        325     217     627    1697     117    1455    1842

  Fragments arranged by size: 

                1842    1697    1455     627     325     217     117
 
BanII G_rGCy'C

Cuts at:   1391    2123    3105    3599    1391 
   Size:        732     982     494    4072

  Fragments arranged by size: 

                4072     982     732     494
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2586     928 
   Size:        971     687    4622

  Fragments arranged by size: 

                4622     971     687
 
BbvCI CC'TCA_GC

Cuts at:   2067    2067 
   Size:       6280
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4471    4505    1903 
   Size:         34    2534      34    3678

  Fragments arranged by size: 

                3678    2534      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   4172    5382    4172 
   Size:       1210    5070
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       6280
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4891     136 
   Size:        107     122      71     631     605    3219    1525

  Fragments arranged by size: 

                3219    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   2968    6275    2968 
   Size:       3307    2973
 
BlpI GC'TnA_GC

Cuts at:   3697    3697 
   Size:       6280
 
Bme1580I G_kGCm'C

Cuts at:   1790    4341    5497    1790 
   Size:       2551    1156    2573

  Fragments arranged by size: 

                2573    2551    1156
 
BmgBI CAC'GTC

Cuts at:   1318    3167    1318 
   Size:       1849    4431
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2361    2895    3082 
   Size:        416      53      83     399      24     944     534     187

Cuts at:   3082    3341    4011    4969     442 
   Size:        259     670     958    1753

  Fragments arranged by size: 

                1753     958     944     670     534     416     399     259
                 187      83      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       6280
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2156    2465    2889    3054    4940    1239 
   Size:        917     309     424     165    1886    2579

  Fragments arranged by size: 

                2579    1886     917     424     309     165
 
Bpu10I CC'TnA_GC

Cuts at:   2067    2067 
   Size:       6280
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2540    4405    5270    5568    2540 
   Size:       1865     865     298    3252

  Fragments arranged by size: 

                3252    1865     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2649    4943     882 
   Size:        617    1150    2294    2219

  Fragments arranged by size: 

                2294    2219    1150     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    4071     493 
   Size:        711    2867    2702

  Fragments arranged by size: 

                2867    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   6274    6274 
   Size:       6280
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    4087    4469    5104     275 
   Size:         53      83     186     936    2554     382     635    1451

  Fragments arranged by size: 

                2554    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   4713    5111    5385    5532    5943    4713 
   Size:        398     274     147     411    5050

  Fragments arranged by size: 

                5050     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    6055

  Fragments arranged by size: 

                6055     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2153    2550    1186 
   Size:        967     397    4916

  Fragments arranged by size: 

                4916     967     397
 
BseYI C'CCAG_C

Cuts at:   2038    5483    5979    2038 
   Size:       3445     496    2339

  Fragments arranged by size: 

                3445    2339     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5707
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3730    4491    5519    1540 
   Size:        275     186    1729     761    1028    2301

  Fragments arranged by size: 

                2301    1729    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2875    3564    4341    4426    5497    2875 
   Size:        689     777      85    1071    3658

  Fragments arranged by size: 

                3658    1071     777     689      85
 
BsmI GAATG_Cn'

Cuts at:   3791    3884    3791 
   Size:         93    6187
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2123    2875    3105    3564    3599    4341    4426 
   Size:        399     333     752     230     459      35     742      85

Cuts at:   4426    5497    1391 
   Size:       1071    2174

  Fragments arranged by size: 

                2174    1071     752     742     459     399     333     230
                  85      35
 
BspEI T'CCGG_A

Cuts at:   5943    5943 
   Size:       6280
 
BspHI T'CATG_A

Cuts at:   2737    4169    5899    2737 
   Size:       1432    1730    3118

  Fragments arranged by size: 

                3118    1730    1432
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3573    3635     844 
   Size:       2729      62    3489

  Fragments arranged by size: 

                3489    2729      62
 
BsrBI CCG'CTC

Cuts at:   1394    1823    4167    1394 
   Size:        429    2344    3507

  Fragments arranged by size: 

                3507    2344     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2394    4775    4949      65 
   Size:       1183    1146    2381     174    1396

  Fragments arranged by size: 

                2381    1396    1183    1146     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4924    5111    1700 
   Size:        110    3114     187    2869

  Fragments arranged by size: 

                3114    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       6280
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       6280
 
BssSI C'ACGA_G

Cuts at:   4340    5352    4340 
   Size:       1012    5268
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       6280
 
BstBI TT'CG_AA

Cuts at:   5084    5084 
   Size:       6280
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       6280
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2620    2620 
   Size:       6280
 
BstYI r'GATC_y

Cuts at:   1972    2968    3610    3977    4362    4379    5831    5917    5929 
   Size:        996     642     367     385      17    1452      86      12

Cuts at:   5929    6275    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     996     642     385     367     346      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2170    2613     513 
   Size:        552    1105     443    4180

  Fragments arranged by size: 

                4180    1105     552     443
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5541

  Fragments arranged by size: 

                5541     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2583    3796    4590    4610    2583 
   Size:       1213     794      20    4253

  Fragments arranged by size: 

                4253    1213     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       6280
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    6245
 
DraI TTT'AAA

Cuts at:   3112    3584    3112 
   Size:        472    5808
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    5287     809 
   Size:        507    3971    1802

  Fragments arranged by size: 

                3971    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2809    3390    3554 
   Size:         54    1425      50     275      18     979     581     164

Cuts at:   3554    3727    4616    6245       8 
   Size:        173     889    1629      43

  Fragments arranged by size: 

                1629    1425     979     889     581     275     173     164
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3727    1537 
   Size:        275    1915    4090

  Fragments arranged by size: 

                4090    1915     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3126    3373    4210    1353 
   Size:       1773     247     837    3423

  Fragments arranged by size: 

                3423    1773     837     247
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4867    5241    5387     387 
   Size:       1064     138    3278     374     146    1280

  Fragments arranged by size: 

                3278    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2156    2465    2681    2889    3054    3150    4343 
   Size:        393     524     309     216     208     165      96    1193

Cuts at:   4343    4940    5727    5995    1239 
   Size:        597     787     268    1524

  Fragments arranged by size: 

                1524    1193     787     597     524     393     309     268
                 216     208     165      96
 
EcoRI G'AATT_C

Cuts at:   3589    3967    3589 
   Size:        378    5902
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2479    3301    3402    2479 
   Size:        822     101    5357

  Fragments arranged by size: 

                5357     822     101
 
FspI TGC'GCA

Cuts at:   4786    4786 
   Size:       6280
 
HaeII r_GCGC'y

Cuts at:   1678    5427    1678 
   Size:       3749    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2105    2137    4921    4953    4995 
   Size:         32     135      32     533      32    2784      32      42

Cuts at:   4995    5027    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2784    2626     533     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3181    3624    3870     669 
   Size:        988     281    1243     443     246    3079

  Fragments arranged by size: 

                3079    1243     988     443     281     246
 
HindIII A'AGCT_T

Cuts at:    748    3531     748 
   Size:       2783    3497
 
HpaI GTT'AAC

Cuts at:   3870    3870 
   Size:       6280
 
KpnI G_GTAC'C

Cuts at:   3605    3605 
   Size:       6280
 
MfeI C'AATT_G

Cuts at:   2907    3879    2907 
   Size:        972    5308
 
MluI A'CGCG_T

Cuts at:   6156    6156 
   Size:       6280
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2161    5394    5578    1305 
   Size:        856    3233     184    2007

  Fragments arranged by size: 

                3233    2007     856     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2811      10 
   Size:         54    1425     343     979    3479

  Fragments arranged by size: 

                3479    1425     979     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2372    2817    4238    4597    4756     518 
   Size:        648    1206     445    1421     359     159    2042

  Fragments arranged by size: 

                2042    1421    1206     648     445     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    3696    4373    5521    5766    1606 
   Size:       2090     677    1148     245    2120

  Fragments arranged by size: 

                2120    2090    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2170    2613     513 
   Size:        552    1105     443    4180

  Fragments arranged by size: 

                4180    1105     552     443
 
NdeI CA'TA_TG

Cuts at:    387    2188    3241     387 
   Size:       1801    1053    3426

  Fragments arranged by size: 

                3426    1801    1053
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       6280
 
NotI GC'GGCC_GC

Cuts at:   3727    3727 
   Size:       6280
 
NspI r_CATG'y

Cuts at:   1132    1229    2863    3638    5183    1132 
   Size:         97    1634     775    1545    2229

  Fragments arranged by size: 

                2229    1634    1545     775      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       6280
 
PciI A'CATG_T

Cuts at:   5179    5179 
   Size:       6280
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2885    1108 
   Size:       1777    4503
 
PmeI GTTT'AAAC

Cuts at:   3584    3584 
   Size:       6280
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4349    4381    5862    5894    4349 
   Size:         32    1481      32    4735

  Fragments arranged by size: 

                4735    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    3176    1613 
   Size:       1563    4717
 
PsiI TTA'TAA

Cuts at:   3850    3850 
   Size:       6280
 
PstI C_TGCA'G

Cuts at:    830    3632     830 
   Size:       2802    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       6280
 
SalI G'TCGA_C

Cuts at:   1936    3179    3622    1936 
   Size:       1243     443    4594

  Fragments arranged by size: 

                4594    1243     443
 
SbfI CC_TGCA'GG

Cuts at:   3632    3632 
   Size:       6280
 
ScaI AGT'ACT

Cuts at:   4528    6240    4528 
   Size:       1712    4568
 
SfcI C'TryA_G

Cuts at:    826    1045    2650    3628    4763    5444    5635     826 
   Size:        219    1605     978    1135     681     191    1471

  Fragments arranged by size: 

                1605    1471    1135     978     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       6280
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       6280
 
SmaI CCC'GGG

Cuts at:   1872    3607    1872 
   Size:       1735    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2519    4384    5285    5547     820 
   Size:        197     926     576    1865     901     262    1553

  Fragments arranged by size: 

                1865    1553     926     901     576     262     197
 
SnaBI TAC'GTA

Cuts at:    493    4071     493 
   Size:       3578    2702
 
SpeI A'CTAG_T

Cuts at:    152    2234     152 
   Size:       2082    4198
 
SphI G_CATG'C

Cuts at:   1132    3638    1132 
   Size:       2506    3774
 
SspI AAT'ATT

Cuts at:      5      52    2361    2440    2571    4204       5 
   Size:         47    2309      79     131    1633    2081

  Fragments arranged by size: 

                2309    2081    1633     131      79      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       6280
 
StyI C'CwwG_G

Cuts at:    513    1065    2170    2613    2689    3719     513 
   Size:        552    1105     443      76    1030    3074

  Fragments arranged by size: 

                3074    1105    1030     552     443      76
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    4307    4324    4477    4662    5107     325 
   Size:       1255    2727      17     153     185     445    1498

  Fragments arranged by size: 

                2727    1498    1255     445     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3256    4526    6238 
   Size:        274      80      33      51    1443    1279    1270    1712

Cuts at:   6238      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1279    1270     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3388    3420    6240    6272    3388 
   Size:         32    2820      32    3396

  Fragments arranged by size: 

                3396    2820      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       6280
 
XbaI T'CTAG_A

Cuts at:   3616    3616 
   Size:       6280
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2177    2177 
   Size:       6280
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       6280
 
XmaI C'CCGG_G

Cuts at:   1870    3605    1870 
   Size:       1735    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2733    3081    4409    2733 
   Size:        348    1328    4604

  Fragments arranged by size: 

                4604    1328     348
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    4088     276 
   Size:         53      83     186     936    2554    2468

  Fragments arranged by size: 

                2554    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI
     MscI     MslI   MspA1I     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PmeI     PpiI    PshAI     PsiI     PstI
     PvuI     SalI     SbfI     ScaI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BfrBI     BplI
    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII  EcoICRI    EcoNI EcoO109I
    EcoRV     FseI    FspAI     KasI     NaeI     NarI   NgoMIV     NruI
     NsiI     PacI     PfoI     PmlI    PpuMI   PspOMI    PspXI     PsrI
    PvuII    RsrII     SacI    SacII    SanDI     SapI    SexAI     SfiI
     SfoI     SrfI     SwaI
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