(Circular) (Six-base) MAPSORT of: pFN21AB4421.seq Check: 4933 from: 1 to: 6280 pFN21AB4421 6280 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3573 3573 Size: 6280 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4090 278 Size: 53 83 186 936 2554 2468 Fragments arranged by size: 2554 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3180 3623 1937 Size: 1243 443 4594 Fragments arranged by size: 4594 1243 443 Acc65I G'GTAC_C Cuts at: 3601 3601 Size: 6280 AclI AA'CG_TT Cuts at: 1563 4407 4780 1563 Size: 2844 373 3063 Fragments arranged by size: 3063 2844 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2681 3150 4343 5727 5995 1632 Size: 1049 469 1193 1384 268 1917 Fragments arranged by size: 1917 1384 1193 1049 469 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6083 AflIII A'CryG_T Cuts at: 1203 3164 5179 6156 1203 Size: 1961 2015 977 1327 Fragments arranged by size: 2015 1961 1327 977 AgeI A'CCGG_T Cuts at: 5111 5111 Size: 6280 AhdI GACnn_n'nnGTC Cuts at: 5009 5009 Size: 6280 AleI CACnn'nnGTG Cuts at: 1166 2817 1166 Size: 1651 4629 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6280 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3282 3314 3282 Size: 32 6248 AlwNI CAG_nnn'CTG Cuts at: 4023 5595 4023 Size: 1572 4708 ApaLI G'TGCA_C Cuts at: 4337 5493 4337 Size: 1156 5124 ApoI r'AATT_y Cuts at: 1493 2216 3353 3589 3815 3967 6076 1493 Size: 723 1137 236 226 152 2109 1697 Fragments arranged by size: 2109 1697 1137 723 236 226 152 AseI AT'TA_AT Cuts at: 160 1032 2849 4834 160 Size: 872 1817 1985 1606 Fragments arranged by size: 1985 1817 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6280 AvaI C'yCGr_G Cuts at: 1870 1943 3605 1870 Size: 73 1662 4545 Fragments arranged by size: 4545 1662 73 BamHI G'GATC_C Cuts at: 3610 3977 3610 Size: 367 5913 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3484 3601 5056 618 Size: 325 217 627 1697 117 1455 1842 Fragments arranged by size: 1842 1697 1455 627 325 217 117 BanII G_rGCy'C Cuts at: 1391 2123 3105 3599 1391 Size: 732 982 494 4072 Fragments arranged by size: 4072 982 732 494 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2586 928 Size: 971 687 4622 Fragments arranged by size: 4622 971 687 BbvCI CC'TCA_GC Cuts at: 2067 2067 Size: 6280 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4471 4505 1903 Size: 34 2534 34 3678 Fragments arranged by size: 3678 2534 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4172 5382 4172 Size: 1210 5070 BclI T'GATC_A Cuts at: 1548 1548 Size: 6280 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4891 136 Size: 107 122 71 631 605 3219 1525 Fragments arranged by size: 3219 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2968 6275 2968 Size: 3307 2973 BlpI GC'TnA_GC Cuts at: 3697 3697 Size: 6280 Bme1580I G_kGCm'C Cuts at: 1790 4341 5497 1790 Size: 2551 1156 2573 Fragments arranged by size: 2573 2551 1156 BmgBI CAC'GTC Cuts at: 1318 3167 1318 Size: 1849 4431 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2361 2895 3082 Size: 416 53 83 399 24 944 534 187 Cuts at: 3082 3341 4011 4969 442 Size: 259 670 958 1753 Fragments arranged by size: 1753 958 944 670 534 416 399 259 187 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6280 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 2465 2889 3054 4940 1239 Size: 917 309 424 165 1886 2579 Fragments arranged by size: 2579 1886 917 424 309 165 Bpu10I CC'TnA_GC Cuts at: 2067 2067 Size: 6280 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2540 4405 5270 5568 2540 Size: 1865 865 298 3252 Fragments arranged by size: 3252 1865 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2649 4943 882 Size: 617 1150 2294 2219 Fragments arranged by size: 2294 2219 1150 617 BsaAI yAC'GTr Cuts at: 493 1204 4071 493 Size: 711 2867 2702 Fragments arranged by size: 2867 2702 711 BsaBI GATnn'nnATC Cuts at: 6274 6274 Size: 6280 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4087 4469 5104 275 Size: 53 83 186 936 2554 382 635 1451 Fragments arranged by size: 2554 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4713 5111 5385 5532 5943 4713 Size: 398 274 147 411 5050 Fragments arranged by size: 5050 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6055 Fragments arranged by size: 6055 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2153 2550 1186 Size: 967 397 4916 Fragments arranged by size: 4916 967 397 BseYI C'CCAG_C Cuts at: 2038 5483 5979 2038 Size: 3445 496 2339 Fragments arranged by size: 3445 2339 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5707 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3730 4491 5519 1540 Size: 275 186 1729 761 1028 2301 Fragments arranged by size: 2301 1729 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2875 3564 4341 4426 5497 2875 Size: 689 777 85 1071 3658 Fragments arranged by size: 3658 1071 777 689 85 BsmI GAATG_Cn' Cuts at: 3791 3884 3791 Size: 93 6187 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2123 2875 3105 3564 3599 4341 4426 Size: 399 333 752 230 459 35 742 85 Cuts at: 4426 5497 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 752 742 459 399 333 230 85 35 BspEI T'CCGG_A Cuts at: 5943 5943 Size: 6280 BspHI T'CATG_A Cuts at: 2737 4169 5899 2737 Size: 1432 1730 3118 Fragments arranged by size: 3118 1730 1432 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3573 3635 844 Size: 2729 62 3489 Fragments arranged by size: 3489 2729 62 BsrBI CCG'CTC Cuts at: 1394 1823 4167 1394 Size: 429 2344 3507 Fragments arranged by size: 3507 2344 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2394 4775 4949 65 Size: 1183 1146 2381 174 1396 Fragments arranged by size: 2381 1396 1183 1146 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4924 5111 1700 Size: 110 3114 187 2869 Fragments arranged by size: 3114 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6280 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6280 BssSI C'ACGA_G Cuts at: 4340 5352 4340 Size: 1012 5268 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6280 BstBI TT'CG_AA Cuts at: 5084 5084 Size: 6280 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6280 BstXI CCAn_nnnn'nTGG Cuts at: 2620 2620 Size: 6280 BstYI r'GATC_y Cuts at: 1972 2968 3610 3977 4362 4379 5831 5917 5929 Size: 996 642 367 385 17 1452 86 12 Cuts at: 5929 6275 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 996 642 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2170 2613 513 Size: 552 1105 443 4180 Fragments arranged by size: 4180 1105 552 443 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5541 Fragments arranged by size: 5541 683 56 BtsI GCAGTG_nn' Cuts at: 2583 3796 4590 4610 2583 Size: 1213 794 20 4253 Fragments arranged by size: 4253 1213 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6280 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6245 DraI TTT'AAA Cuts at: 3112 3584 3112 Size: 472 5808 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5287 809 Size: 507 3971 1802 Fragments arranged by size: 3971 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2809 3390 3554 Size: 54 1425 50 275 18 979 581 164 Cuts at: 3554 3727 4616 6245 8 Size: 173 889 1629 43 Fragments arranged by size: 1629 1425 979 889 581 275 173 164 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3727 1537 Size: 275 1915 4090 Fragments arranged by size: 4090 1915 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3126 3373 4210 1353 Size: 1773 247 837 3423 Fragments arranged by size: 3423 1773 837 247 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4867 5241 5387 387 Size: 1064 138 3278 374 146 1280 Fragments arranged by size: 3278 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2465 2681 2889 3054 3150 4343 Size: 393 524 309 216 208 165 96 1193 Cuts at: 4343 4940 5727 5995 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1193 787 597 524 393 309 268 216 208 165 96 EcoRI G'AATT_C Cuts at: 3589 3967 3589 Size: 378 5902 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2479 3301 3402 2479 Size: 822 101 5357 Fragments arranged by size: 5357 822 101 FspI TGC'GCA Cuts at: 4786 4786 Size: 6280 HaeII r_GCGC'y Cuts at: 1678 5427 1678 Size: 3749 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2105 2137 4921 4953 4995 Size: 32 135 32 533 32 2784 32 42 Cuts at: 4995 5027 1373 Size: 32 2626 Fragments arranged by size: 2784 2626 533 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3181 3624 3870 669 Size: 988 281 1243 443 246 3079 Fragments arranged by size: 3079 1243 988 443 281 246 HindIII A'AGCT_T Cuts at: 748 3531 748 Size: 2783 3497 HpaI GTT'AAC Cuts at: 3870 3870 Size: 6280 KpnI G_GTAC'C Cuts at: 3605 3605 Size: 6280 MfeI C'AATT_G Cuts at: 2907 3879 2907 Size: 972 5308 MluI A'CGCG_T Cuts at: 6156 6156 Size: 6280 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2161 5394 5578 1305 Size: 856 3233 184 2007 Fragments arranged by size: 3233 2007 856 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2811 10 Size: 54 1425 343 979 3479 Fragments arranged by size: 3479 1425 979 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2372 2817 4238 4597 4756 518 Size: 648 1206 445 1421 359 159 2042 Fragments arranged by size: 2042 1421 1206 648 445 359 159 MspA1I CmG'CkG Cuts at: 1606 3696 4373 5521 5766 1606 Size: 2090 677 1148 245 2120 Fragments arranged by size: 2120 2090 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2170 2613 513 Size: 552 1105 443 4180 Fragments arranged by size: 4180 1105 552 443 NdeI CA'TA_TG Cuts at: 387 2188 3241 387 Size: 1801 1053 3426 Fragments arranged by size: 3426 1801 1053 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6280 NotI GC'GGCC_GC Cuts at: 3727 3727 Size: 6280 NspI r_CATG'y Cuts at: 1132 1229 2863 3638 5183 1132 Size: 97 1634 775 1545 2229 Fragments arranged by size: 2229 1634 1545 775 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6280 PciI A'CATG_T Cuts at: 5179 5179 Size: 6280 PflMI CCAn_nnn'nTGG Cuts at: 1108 2885 1108 Size: 1777 4503 PmeI GTTT'AAAC Cuts at: 3584 3584 Size: 6280 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4349 4381 5862 5894 4349 Size: 32 1481 32 4735 Fragments arranged by size: 4735 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3176 1613 Size: 1563 4717 PsiI TTA'TAA Cuts at: 3850 3850 Size: 6280 PstI C_TGCA'G Cuts at: 830 3632 830 Size: 2802 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6280 SalI G'TCGA_C Cuts at: 1936 3179 3622 1936 Size: 1243 443 4594 Fragments arranged by size: 4594 1243 443 SbfI CC_TGCA'GG Cuts at: 3632 3632 Size: 6280 ScaI AGT'ACT Cuts at: 4528 6240 4528 Size: 1712 4568 SfcI C'TryA_G Cuts at: 826 1045 2650 3628 4763 5444 5635 826 Size: 219 1605 978 1135 681 191 1471 Fragments arranged by size: 1605 1471 1135 978 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6280 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6280 SmaI CCC'GGG Cuts at: 1872 3607 1872 Size: 1735 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2519 4384 5285 5547 820 Size: 197 926 576 1865 901 262 1553 Fragments arranged by size: 1865 1553 926 901 576 262 197 SnaBI TAC'GTA Cuts at: 493 4071 493 Size: 3578 2702 SpeI A'CTAG_T Cuts at: 152 2234 152 Size: 2082 4198 SphI G_CATG'C Cuts at: 1132 3638 1132 Size: 2506 3774 SspI AAT'ATT Cuts at: 5 52 2361 2440 2571 4204 5 Size: 47 2309 79 131 1633 2081 Fragments arranged by size: 2309 2081 1633 131 79 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6280 StyI C'CwwG_G Cuts at: 513 1065 2170 2613 2689 3719 513 Size: 552 1105 443 76 1030 3074 Fragments arranged by size: 3074 1105 1030 552 443 76 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4307 4324 4477 4662 5107 325 Size: 1255 2727 17 153 185 445 1498 Fragments arranged by size: 2727 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3256 4526 6238 Size: 274 80 33 51 1443 1279 1270 1712 Cuts at: 6238 96 Size: 138 Fragments arranged by size: 1712 1443 1279 1270 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3388 3420 6240 6272 3388 Size: 32 2820 32 3396 Fragments arranged by size: 3396 2820 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6280 XbaI T'CTAG_A Cuts at: 3616 3616 Size: 6280 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2177 2177 Size: 6280 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6280 XmaI C'CCGG_G Cuts at: 1870 3605 1870 Size: 1735 4545 XmnI GAAnn'nnTTC Cuts at: 2733 3081 4409 2733 Size: 348 1328 4604 Fragments arranged by size: 4604 1328 348 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4088 276 Size: 53 83 186 936 2554 2468 Fragments arranged by size: 2554 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI