Restriction map for
pFN21AB4421
(Linear) (Six-base) MAPSORT of: KIBB4421.seq Check: 2747 from: 1 to: 1575
KIBB4421.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1568 1575
Size: 1568 7
AccI GT'mk_AC
Cuts at: 0 1175 1575
Size: 1175 400
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 1145 1575
Size: 676 469 430
Fragments arranged by size:
676 469 430
AflIII A'CryG_T
Cuts at: 0 1159 1575
Size: 1159 416
AleI CACnn'nnGTG
Cuts at: 0 812 1575
Size: 812 763
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1277 1309 1575
Size: 1277 32 266
Fragments arranged by size:
1277 266 32
ApoI r'AATT_y
Cuts at: 0 211 1348 1575
Size: 211 1137 227
Fragments arranged by size:
1137 227 211
AseI AT'TA_AT
Cuts at: 0 844 1575
Size: 844 731
BanI G'GyrC_C
Cuts at: 0 1479 1575
Size: 1479 96
BanII G_rGCy'C
Cuts at: 0 118 1100 1575
Size: 118 982 475
Fragments arranged by size:
982 475 118
BbsI GAAGACnn'nnnn_
Cuts at: 0 581 1575
Size: 581 994
BbvCI CC'TCA_GC
Cuts at: 0 62 1575
Size: 62 1513
BglII A'GATC_T
Cuts at: 0 963 1575
Size: 963 612
BmgBI CAC'GTC
Cuts at: 0 1162 1575
Size: 1162 413
BmrI ACTGGGnnnn_n'
Cuts at: 0 356 890 1077 1336 1575
Size: 356 534 187 259 239
Fragments arranged by size:
534 356 259 239 187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 460 884 1049 1575
Size: 151 309 424 165 526
Fragments arranged by size:
526 424 309 165 151
Bpu10I CC'TnA_GC
Cuts at: 0 62 1575
Size: 62 1513
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 1575
Size: 535 1040
BsaI GGTCTCn'nnnn_
Cuts at: 0 644 1575
Size: 644 931
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 545 1575
Size: 148 397 1030
Fragments arranged by size:
1030 397 148
BseYI C'CCAG_C
Cuts at: 0 33 1575
Size: 33 1542
BsiHKAI G_wGCw'C
Cuts at: 0 870 1559 1575
Size: 870 689 16
Fragments arranged by size:
870 689 16
Bsp1286I G_dGCh'C
Cuts at: 0 118 870 1100 1559 1575
Size: 118 752 230 459 16
Fragments arranged by size:
752 459 230 118 16
BspHI T'CATG_A
Cuts at: 0 732 1575
Size: 732 843
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1568 1575
Size: 1568 7
BsrDI GCAATG_nn'
Cuts at: 0 389 1575
Size: 389 1186
BstXI CCAn_nnnn'nTGG
Cuts at: 0 615 1575
Size: 615 960
BstYI r'GATC_y
Cuts at: 0 963 1575
Size: 963 612
BtgI C'CryG_G
Cuts at: 0 165 608 1575
Size: 165 443 967
Fragments arranged by size:
967 443 165
BtsI GCAGTG_nn'
Cuts at: 0 578 1575
Size: 578 997
DraI TTT'AAA
Cuts at: 0 1107 1575
Size: 1107 468
EaeI y'GGCC_r
Cuts at: 0 804 1385 1549 1575
Size: 804 581 164 26
Fragments arranged by size:
804 581 164 26
EarI CTCTTCn'nnn_
Cuts at: 0 1121 1368 1575
Size: 1121 247 207
Fragments arranged by size:
1121 247 207
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 460 676 884 1049 1145 1575
Size: 151 309 216 208 165 96 430
Fragments arranged by size:
430 309 216 208 165 151 96
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 474 1296 1397 1575
Size: 474 822 101 178
Fragments arranged by size:
822 474 178 101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 100 132 1575
Size: 100 32 1443
Fragments arranged by size:
1443 100 32
HincII GTy'rAC
Cuts at: 0 1176 1575
Size: 1176 399
HindIII A'AGCT_T
Cuts at: 0 1526 1575
Size: 1526 49
MfeI C'AATT_G
Cuts at: 0 902 1575
Size: 902 673
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 156 1575
Size: 156 1419
MscI TGG'CCA
Cuts at: 0 806 1575
Size: 806 769
MslI CAynn'nnrTG
Cuts at: 0 367 812 1575
Size: 367 445 763
Fragments arranged by size:
763 445 367
NcoI C'CATG_G
Cuts at: 0 165 608 1575
Size: 165 443 967
Fragments arranged by size:
967 443 165
NdeI CA'TA_TG
Cuts at: 0 183 1236 1575
Size: 183 1053 339
Fragments arranged by size:
1053 339 183
NspI r_CATG'y
Cuts at: 0 858 1575
Size: 858 717
PflMI CCAn_nnn'nTGG
Cuts at: 0 880 1575
Size: 880 695
PshAI GACnn'nnGTC
Cuts at: 0 1171 1575
Size: 1171 404
SalI G'TCGA_C
Cuts at: 0 1174 1575
Size: 1174 401
SfcI C'TryA_G
Cuts at: 0 645 1575
Size: 645 930
SmlI C'TyrA_G
Cuts at: 0 514 1575
Size: 514 1061
SpeI A'CTAG_T
Cuts at: 0 229 1575
Size: 229 1346
SspI AAT'ATT
Cuts at: 0 356 435 566 1575
Size: 356 79 131 1009
Fragments arranged by size:
1009 356 131 79
StyI C'CwwG_G
Cuts at: 0 165 608 684 1575
Size: 165 443 76 891
Fragments arranged by size:
891 443 165 76
TatI w'GTAC_w
Cuts at: 0 1251 1575
Size: 1251 324
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1383 1415 1575
Size: 1383 32 160
Fragments arranged by size:
1383 160 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 172 1575
Size: 172 1403
XmnI GAAnn'nnTTC
Cuts at: 0 728 1076 1575
Size: 728 348 499
Fragments arranged by size:
728 499 348
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AloI ApoI AseI
BanI BanII BbsI BbvCI BglII BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BseRI BseYI BsiHKAI Bsp1286I BspHI
BspMI BsrDI BstXI BstYI BtgI BtsI DraI EaeI
EarI Eco57MI FalI Hin4I HincII HindIII MfeI MmeI
MscI MslI NcoI NdeI NspI PflMI PshAI SalI
SfcI SmlI SpeI SspI StyI TatI TstI XcmI
XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AlfI
AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BbeI BcgI BciVI BclI BfrBI BglI BlpI
Bme1580I BmtI BplI BsaAI BsaBI BsaHI BsaWI BsaXI
BsgI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII
HpaI KasI KpnI MluI MspA1I NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SphI SrfI StuI SwaI TaqII Tth111I XbaI
XhoI XmaI ZraI