Restriction map for
pFN21AB4380
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI AocI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AvrII BamHI BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmAI BsmBI BsmI BspDI
BspEI BspHI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HpaI KasI KpnI Ksp632I MamI
MfeI MflI MluI MroI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SalI SapI
SbfI ScaI SexAI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI TaqI TfiI
Tsp509I TthHB8I Van91I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 29 enzymes) #
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AfaI 675 GTAC GT^AC
ApaI 84 GGGCCC GGGCC^C
AspI 409 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 749 TGCGCA TGC^GCA
BbvCI 1017 CCTCAGC CCTCAGC(-5/-2)
BglI 760 GCCCTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1117 CTGGAG CTGGAG(16/14)
Bsp1407I 674 TGTACA T^GTACA
BspLU11I 419 ACATGT A^CATGT
BspMI 1147 ACCTGC ACCTGC(4/8)
BsrBI 644 CCGCTC CCGCTC(-3/-3)
BsrGI 674 TGTACA T^GTACA
BsrI 365 ACTGG ACTGG(1/-1)
BssSI 394 CTCGTG CACGAG(-5/-1)
BstAPI 1007 GCATCCTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
EcoT14I 521 CCAAGG C^C[AT][AT]GG
FspI 749 TGCGCA TGC^GCA
MaeIII 343 GTCAC ^GT[ACGT]AC
MseI 811 TTAA T^TAA
PflFI 409 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 145 GGGACCT [GA]G^G[AT]CC[CT]
RsaI 675 GTAC GT^AC
SfcI 1086 CTACAG C^T[GA][CT]AG
SfiI 759 GGCCCTCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 674 TGTACA T^GTACA
StyI 521 CCAAGG C^C[AT][AT]GG
Tru9I 811 TTAA T^TAA
Tsp45I 343 GTCAC ^GT[GC]AC
Tth111I 409 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 901 GACGCC G[GA]^CG[CT]C
1129 GGCGTC
AflIII 419 ACATGT A^C[GA][CT]GT
881 ACGTGT
AlwI 246 GATCC GGATC(4/5)
1160 GGATC
Aor51HI 788 AGCGCT AGC^GCT
992 AGCGCT
AvaI 51 CCCGGG C^[CT]CG[GA]G
88 CCCGGG
AvaII 146 GGACC G^G[AT]CC
459 GGACC
BalI 299 TGGCCA TGG^CCA
518 TGGCCA
BanI 13 GGCACC G^G[CT][GA]CC
1144 GGCACC
BanII 84 GGGCCC G[GA]GC[CT]^C
640 GAGCCC
BfaI 41 CTAG C^TAG
340 CTAG
BsaHI 901 GACGCC G[GA]^CG[CT]C
1129 GGCGTC
BsgI 1149 CTGCAC GTGCAG(16/14)
868 GTGCAG
BsoBI 51 CCCGGG C^[CT]CG[GA]G
88 CCCGGG
BsrDI 303 CATTGC GCAATG(2/0)
738 CATTGC
Eco47III 788 AGCGCT AGC^GCT
992 AGCGCT
FauI 497 CCCGC CCCGC(4/6)
643 CCCGC
Hin1I 901 GACGCC G[GA]^CG[CT]C
1129 GGCGTC
HinfI 912 GAGTC G^A[ACGT]TC
1120 GAGTC
KspI 593 CCGCGG CCGC^GG
1037 CCGCGG
MaeI 41 CTAG C^TAG
340 CTAG
MluNI 299 TGGCCA TGG^CCA
518 TGGCCA
MscI 299 TGGCCA TGG^CCA
518 TGGCCA
NspI 419 ACATGT [GA]CATG^[CT]
626 ACATGC
PleI 912 GAGTC GAGTC(4/5)
1120 GAGTC
SacII 593 CCGCGG CCGC^GG
1037 CCGCGG
SmaI 51 CCCGGG CCC^GGG
88 CCCGGG
TspRI 364 CACTG CA[GC]TG[ACGT][ACGT]^
1089 CAGTG
XmaCI 51 CCCGGG C^CCGGG
88 CCCGGG
XmaI 51 CCCGGG C^CCGGG
88 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AluI AspHI BbsI
BbvI BcnI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrFI
BssKI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HpaII HphI ItaI MaeII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NciI NdeII NgoMIV NlaIII NlaIV
PvuII Sau3AI Sau96I ScrFI SfaNI
SmlI TseI