Restriction map for
pFN21AB4380
(Linear) (Six-base) MAPSORT of: KIBB4380.seq Check: 5667 from: 1 to: 1173
KIBB4380.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1156 1173
Size: 1156 17
AfeI AGC'GCT
Cuts at: 0 790 994 1173
Size: 790 204 179
Fragments arranged by size:
790 204 179
AflIII A'CryG_T
Cuts at: 0 419 881 1173
Size: 419 462 292
Fragments arranged by size:
462 419 292
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1105 1137 1173
Size: 1105 32 36
Fragments arranged by size:
1105 36 32
ApaI G_GGCC'C
Cuts at: 0 88 1173
Size: 88 1085
AvaI C'yCGr_G
Cuts at: 0 51 88 1173
Size: 51 37 1085
Fragments arranged by size:
1085 51 37
BanI G'GyrC_C
Cuts at: 0 13 1144 1173
Size: 13 1131 29
Fragments arranged by size:
1131 29 13
BanII G_rGCy'C
Cuts at: 0 88 644 1173
Size: 88 556 529
Fragments arranged by size:
556 529 88
BbsI GAAGACnn'nnnn_
Cuts at: 0 274 573 1103 1125 1173
Size: 274 299 530 22 48
Fragments arranged by size:
530 299 274 48 22
BbvCI CC'TCA_GC
Cuts at: 0 1018 1173
Size: 1018 155
BglI GCCn_nnn'nGGC
Cuts at: 0 766 1173
Size: 766 407
BlpI GC'TnA_GC
Cuts at: 0 325 400 451 890 1173
Size: 325 75 51 439 283
Fragments arranged by size:
439 325 283 75 51
Bme1580I G_kGCm'C
Cuts at: 0 88 335 1173
Size: 88 247 838
Fragments arranged by size:
838 247 88
BmgBI CAC'GTC
Cuts at: 0 1154 1173
Size: 1154 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1138 1173
Size: 1138 35
Bpu10I CC'TnA_GC
Cuts at: 0 1018 1173
Size: 1018 155
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 748 1057 1173
Size: 171 577 309 116
Fragments arranged by size:
577 309 171 116
BsaAI yAC'GTr
Cuts at: 0 243 468 729 864 1173
Size: 243 225 261 135 309
Fragments arranged by size:
309 261 243 225 135
BsaHI Gr'CG_yC
Cuts at: 0 902 1130 1173
Size: 902 228 43
Fragments arranged by size:
902 228 43
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 12 24 130 1057 1173
Size: 12 12 106 927 116
Fragments arranged by size:
927 116 106 12 12
BseYI C'CCAG_C
Cuts at: 0 46 1173
Size: 46 1127
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 889 1134 1173
Size: 889 245 39
Fragments arranged by size:
889 245 39
BsiHKAI G_wGCw'C
Cuts at: 0 401 452 734 1173
Size: 401 51 282 439
Fragments arranged by size:
439 401 282 51
Bsp1286I G_dGCh'C
Cuts at: 0 88 335 401 452 644 734 1173
Size: 88 247 66 51 192 90 439
Fragments arranged by size:
439 247 192 90 88 66 51
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1156 1173
Size: 1156 17
BsrBI CCG'CTC
Cuts at: 0 646 1173
Size: 646 527
BsrDI GCAATG_nn'
Cuts at: 0 302 737 1173
Size: 302 435 436
Fragments arranged by size:
436 435 302
BsrFI r'CCGG_y
Cuts at: 0 94 506 769 1047 1173
Size: 94 412 263 278 126
Fragments arranged by size:
412 278 263 126 94
BsrGI T'GTAC_A
Cuts at: 0 674 1173
Size: 674 499
BssSI C'ACGA_G
Cuts at: 0 394 1173
Size: 394 779
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1013 1173
Size: 1013 160
BtgI C'CryG_G
Cuts at: 0 197 473 515 593 704 905 1037 1173
Size: 197 276 42 78 111 201 132 136
Fragments arranged by size:
276 201 197 136 132 111 78 42
EaeI y'GGCC_r
Cuts at: 0 272 299 470 518 596 1173
Size: 272 27 171 48 78 577
Fragments arranged by size:
577 272 171 78 48 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1138 1173
Size: 1138 35
EcoO109I rG'GnC_Cy
Cuts at: 0 84 85 146 1173
Size: 84 1 61 1027
Fragments arranged by size:
1027 84 61 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 825 1173
Size: 825 348
FspI TGC'GCA
Cuts at: 0 751 1173
Size: 751 422
FspAI rTGC'GCAy
Cuts at: 0 751 1173
Size: 751 422
HaeII r_GCGC'y
Cuts at: 0 101 173 792 996 1173
Size: 101 72 619 204 177
Fragments arranged by size:
619 204 177 101 72
HincII GTy'rAC
Cuts at: 0 417 879 1053 1173
Size: 417 462 174 120
Fragments arranged by size:
462 417 174 120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1026 1128 1173
Size: 1026 102 45
Fragments arranged by size:
1026 102 45
MscI TGG'CCA
Cuts at: 0 301 520 1173
Size: 301 219 653
Fragments arranged by size:
653 301 219
MslI CAynn'nnrTG
Cuts at: 0 241 631 811 1173
Size: 241 390 180 362
Fragments arranged by size:
390 362 241 180
MspA1I CmG'CkG
Cuts at: 0 59 595 873 918 947 1022 1039 1173
Size: 59 536 278 45 29 75 17 134
Fragments arranged by size:
536 278 134 75 59 45 29 17
NaeI GCC'GGC
Cuts at: 0 96 508 771 1173
Size: 96 412 263 402
Fragments arranged by size:
412 402 263 96
NgoMIV G'CCGG_C
Cuts at: 0 94 506 769 1173
Size: 94 412 263 404
Fragments arranged by size:
412 404 263 94
NspI r_CATG'y
Cuts at: 0 423 630 1173
Size: 423 207 543
Fragments arranged by size:
543 423 207
PciI A'CATG_T
Cuts at: 0 419 1173
Size: 419 754
PfoI T'CCnGG_A
Cuts at: 0 1156 1173
Size: 1156 17
PpuMI rG'GwC_Cy
Cuts at: 0 146 1173
Size: 146 1027
PspOMI G'GGCC_C
Cuts at: 0 84 1173
Size: 84 1089
PvuII CAG'CTG
Cuts at: 0 59 873 918 947 1022 1173
Size: 59 814 45 29 75 151
Fragments arranged by size:
814 151 75 59 45 29
SacII CC_GC'GG
Cuts at: 0 596 1040 1173
Size: 596 444 133
Fragments arranged by size:
596 444 133
SfcI C'TryA_G
Cuts at: 0 1086 1173
Size: 1086 87
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 766 1173
Size: 766 407
SmaI CCC'GGG
Cuts at: 0 53 90 1173
Size: 53 37 1083
Fragments arranged by size:
1083 53 37
SmlI C'TyrA_G
Cuts at: 0 150 763 1072 1173
Size: 150 613 309 101
Fragments arranged by size:
613 309 150 101
SrfI GCCC'GGGC
Cuts at: 0 53 1173
Size: 53 1120
StyI C'CwwG_G
Cuts at: 0 521 1173
Size: 521 652
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 534 912 1173
Size: 534 378 261
Fragments arranged by size:
534 378 261
TatI w'GTAC_w
Cuts at: 0 674 1173
Size: 674 499
Tth111I GACn'n_nGTC
Cuts at: 0 412 1173
Size: 412 761
XmaI C'CCGG_G
Cuts at: 0 51 88 1173
Size: 51 37 1085
Fragments arranged by size:
1085 51 37
Enzymes that do cut:
AarI AfeI AflIII AloI ApaI AvaI BanI BanII
BbsI BbvCI BglI BlpI Bme1580I BmgBI BpmI Bpu10I
BpuEI BsaAI BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BtgI
EaeI Eco57MI EcoO109I FalI FspI FspAI HaeII HincII
MmeI MscI MslI MspA1I NaeI NgoMIV NspI PciI
PfoI PpuMI PspOMI PvuII SacII SfcI SfiI SmaI
SmlI SrfI StyI TaqII TatI Tth111I XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI
BglII BmrI BmtI BplI BsaI BsaBI BsaWI BsaXI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BssHII BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FseI Hin4I HindIII HpaI KasI
KpnI MfeI MluI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfoI SgfI
SgrAI SnaBI SpeI SphI SspI StuI SwaI TstI
XbaI XcmI XhoI XmnI ZraI