Restriction map for
pFN21AB4329
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccII AclI AflII
AflIII AgeI Alw44I ApaLI AscI
Asp700I Asp718I AspI AviII BbrPI
BbvCI BcgI BclI BfrI BglII
BlpI Bpu1102I BsiEI BsiWI Bsp1407I
BspLU11I BsrFI BsrGI BssHII BssSI
Bst1107I BstEII BstPI BstUI BstZ17I
CelII Cfr10I CpoI DraII EagI
EarI EclXI Eco52I EcoNI EcoO109I
EcoO65I EcoT22I FbaI FseI FspI
HgaI HpaI KpnI Ksp632I KspI
MluI MvnI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PflFI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI
SgrAI SmaI SpeI SphI Sse8387I
SspBI Tth111I XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccIII 2176 TCCGGA T^CCGGA
AcsI 1760 GAATTC [GA]^AATT[CT]
AcyI 1580 GGCGCC G[GA]^CG[CT]C
AhdI 670 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1057 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1166 AGCGCT AGC^GCT
ApaI 2373 GGGCCC GGGCC^C
ApoI 1760 GAATTC [GA]^AATT[CT]
AseI 610 ATTAAT AT^TAAT
AsnI 610 ATTAAT AT^TAAT
AspEI 670 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1331 CTCGAG C^[CT]CG[GA]G
AvrII 1965 CCTAGG C^CTAGG
BamHI 399 GGATCC G^GATCC
BbeI 1580 GGCGCC GGCGC^C
BciVI 2711 GTATCC GTATCC(6/5)
BlnI 1965 CCTAGG C^CTAGG
BsaAI 2708 TACGTA [CT]AC^GT[GA]
BsaHI 1580 GGCGCC G[GA]^CG[CT]C
BsaI 1559 GAGACC GGTCTC(1/5)
BsaWI 2176 TCCGGA [AT]^CCGG[AT]
BseAI 2176 TCCGGA T^CCGGA
BsmBI 1651 GAGACG CGTCTC(1/5)
BsmI 637 GAATGC GAATGC(1/-1)
BsoBI 1331 CTCGAG C^[CT]CG[GA]G
BspDI 2431 ATCGAT AT^CGAT
BspEI 2176 TCCGGA T^CCGGA
BstBI 1109 TTCGAA TT^CGAA
Cac8I 1122 GCGGGC GC[ACGT]^[ACGT]GC
ClaI 2431 ATCGAT AT^CGAT
DrdI 1976 GACTAGATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 670 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1166 AGCGCT AGC^GCT
EcoRI 1760 GAATTC G^AATTC
EcoRV 448 GATATC GAT^ATC
Hin1I 1580 GGCGCC G[GA]^CG[CT]C
HincII 1099 GTTGAC GT[CT]^[GA]AC
HindII 1099 GTTGAC GT[CT]^[GA]AC
KasI 1580 GGCGCC G^GCGCC
MfeI 1229 CAATTG C^AATTG
MroI 2176 TCCGGA T^CCGGA
MunI 1229 CAATTG C^AATTG
NarI 1580 GGCGCC GG^CGCC
NcoI 326 CCATGG C^CATGG
NspI 2654 ACATGC [GA]CATG^[CT]
NspV 1109 TTCGAA TT^CGAA
PaeR7I 1331 CTCGAG C^TCGAG
PflMI 459 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1903 GACCTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 610 ATTAAT AT^TAAT
PvuII 2448 CAGCTG CAG^CTG
SacI 2756 GAGCTC GAGCT^C
SexAI 2109 ACCTGGT A^CC[AT]GGT
SfoI 1580 GGCGCC GGC^GCC
SfuI 1109 TTCGAA TT^CGAA
SnaBI 2708 TACGTA TAC^GTA
SspI 42 AATATT AAT^ATT
StuI 937 AGGCCT AGG^CCT
SwaI 2573 ATTTAAAT ATTT^AAAT
Van91I 459 CCAAATGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 798 TCTAGA T^CTAGA
XhoI 1331 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 343 GTAGAC GT^[AC][GT]AC
2788 GTCTAC
AfaI 981 GTAC GT^AC
2661 GTAC
AspHI 1794 GAGCAC G[AT]GC[AT]^C
2756 GAGCTC
BalI 248 TGGCCA TGG^CCA
2309 TGGCCA
BanI 1580 GGCGCC G^G[CT][GA]CC
2106 GGCACC
BanII 2373 GGGCCC G[GA]GC[CT]^C
2756 GAGCTC
BpmI 2407 CTCCAG CTGGAG(16/14)
1408 CTGGAG
BsgI 892 GTGCAG GTGCAG(16/14)
1438 GTGCAG
BsiHKAI 1794 GAGCAC G[AT]GC[AT]^C
2756 GAGCTC
BspHI 1022 TCATGA T^CATGA
2381 TCATGA
BsrBI 373 CCGCTC CCGCTC(-3/-3)
724 GAGCGG
BsrDI 1050 CATTGC GCAATG(2/0)
1181 CATTGC
BstAPI 2023 GCAACCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2649 GCAGGACATGC
BstXI 1142 CCATCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1940 CCATGCAGATGG
DraI 1893 TTTAAA TTT^AAA
2574 TTTAAA
DraIII 1290 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
2108 CACCTGGTG
DsaI 125 CCGTGG C^C[GA][CT]GG
326 CCATGG
FauI 726 GCGGG CCCGC(4/6)
1122 GCGGG
HindIII 199 AAGCTT A^AGCTT
1401 AAGCTT
MaeII 158 ACGT A^CGT
2709 ACGT
MluNI 248 TGGCCA TGG^CCA
2309 TGGCCA
MscI 248 TGGCCA TGG^CCA
2309 TGGCCA
MspA1I 773 CAGCGG C[AC]G^C[GT]G
2448 CAGCTG
RcaI 1022 TCATGA T^CATGA
2381 TCATGA
RsaI 981 GTAC GT^AC
2661 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AluI AlwI AocI AvaII
BbsI BbvI BcnI BfaI BglI
BmyI BpuAI BsaBI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstYI Bsu36I CfoI Cfr13I DdeI
DpnII EaeI Eco81I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII