(Circular) (Six-base) MAPSORT of: pFN21AB4329.seq Check: 3396 from: 1 to: 7516 pFN21AB4329 7516 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3800 4060 3800 Size: 260 7256 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5326 278 Size: 53 83 186 936 3790 2468 Fragments arranged by size: 3790 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2349 4794 4859 1937 Size: 412 2445 65 4594 Fragments arranged by size: 4594 2445 412 65 Acc65I G'GTAC_C Cuts at: 4837 4837 Size: 7516 AclI AA'CG_TT Cuts at: 1563 4815 5643 6016 1563 Size: 3252 828 373 3063 Fragments arranged by size: 3252 3063 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2867 4435 4538 4697 4772 4788 5579 6963 Size: 1235 1568 103 159 75 16 791 1384 Cuts at: 6963 7231 1632 Size: 268 1917 Fragments arranged by size: 1917 1568 1384 1235 791 268 159 103 75 16 AfeI AGC'GCT Cuts at: 3173 3173 Size: 7516 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7319 AflIII A'CryG_T Cuts at: 1203 6415 7392 1203 Size: 5212 977 1327 Fragments arranged by size: 5212 1327 977 AgeI A'CCGG_T Cuts at: 6347 6347 Size: 7516 AhdI GACnn_n'nnGTC Cuts at: 2680 6245 2680 Size: 3565 3951 AleI CACnn'nnGTG Cuts at: 1166 3440 4753 1166 Size: 2274 1313 3929 Fragments arranged by size: 3929 2274 1313 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2449 3458 1184 Size: 1265 1009 5242 Fragments arranged by size: 5242 1265 1009 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4701 4733 4701 Size: 32 7484 AlwNI CAG_nnn'CTG Cuts at: 3067 5259 6831 3067 Size: 2192 1572 3752 Fragments arranged by size: 3752 2192 1572 ApaI G_GGCC'C Cuts at: 4382 4382 Size: 7516 ApaLI G'TGCA_C Cuts at: 5573 6729 5573 Size: 1156 6360 ApoI r'AATT_y Cuts at: 1493 3765 4825 5051 5203 7312 1493 Size: 2272 1060 226 152 2109 1697 Fragments arranged by size: 2272 2109 1697 1060 226 152 AseI AT'TA_AT Cuts at: 160 1032 2616 6070 160 Size: 872 1584 3454 1606 Fragments arranged by size: 3454 1606 1584 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7516 AvaI C'yCGr_G Cuts at: 1870 1943 3336 4841 1870 Size: 73 1393 1505 4545 Fragments arranged by size: 4545 1505 1393 73 AvrII C'CTAG_G Cuts at: 3970 3970 Size: 7516 BamHI G'GATC_C Cuts at: 2404 4846 5213 2404 Size: 2442 367 4707 Fragments arranged by size: 4707 2442 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3585 4111 4837 6292 618 Size: 325 217 627 1798 526 726 1455 1842 Fragments arranged by size: 1842 1798 1455 726 627 526 325 217 BanII G_rGCy'C Cuts at: 1391 4382 4765 4835 1391 Size: 2991 383 70 4072 Fragments arranged by size: 4072 2991 383 70 BbeI G_GCGC'C Cuts at: 3589 3589 Size: 7516 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2965 4435 4504 928 Size: 971 1066 1470 69 3940 Fragments arranged by size: 3940 1470 1066 971 69 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5707 5741 1903 Size: 34 3770 34 3678 Fragments arranged by size: 3770 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4727 5408 6618 4727 Size: 681 1210 5625 Fragments arranged by size: 5625 1210 681 BclI T'GATC_A Cuts at: 1548 1548 Size: 7516 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2261 2549 3825 Size: 107 122 71 631 605 589 288 1276 Cuts at: 3825 6127 136 Size: 2302 1525 Fragments arranged by size: 2302 1525 1276 631 605 589 288 122 107 71 BglII A'GATC_T Cuts at: 7511 7511 Size: 7516 BlpI GC'TnA_GC Cuts at: 4933 4933 Size: 7516 Bme1580I G_kGCm'C Cuts at: 1790 4209 4382 5577 6733 1790 Size: 2419 173 1195 1156 2573 Fragments arranged by size: 2573 2419 1195 1156 173 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7516 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3914 4376 5247 Size: 416 53 83 399 24 2497 462 871 Cuts at: 5247 6205 442 Size: 958 1753 Fragments arranged by size: 2497 1753 958 871 462 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7516 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3434 4397 6176 1239 Size: 2195 963 1779 2579 Fragments arranged by size: 2579 2195 1779 963 Bpu10I CC'TnA_GC Cuts at: 3060 3060 Size: 7516 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3030 3467 3544 3858 5641 6506 6804 3030 Size: 437 77 314 1783 865 298 3742 Fragments arranged by size: 3742 1783 865 437 314 298 77 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3558 6179 882 Size: 617 2059 2621 2219 Fragments arranged by size: 2621 2219 2059 617 BsaAI yAC'GTr Cuts at: 493 1204 4715 5307 493 Size: 711 3511 592 2702 Fragments arranged by size: 3511 2702 711 592 BsaBI GATnn'nnATC Cuts at: 2298 2403 2520 2666 7510 2298 Size: 105 117 146 4844 2304 Fragments arranged by size: 4844 2304 146 117 105 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3586 5323 5705 6340 Size: 53 83 186 936 2053 1737 382 635 Cuts at: 6340 275 Size: 1451 Fragments arranged by size: 2053 1737 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4181 5949 6347 6621 6768 7179 4181 Size: 1768 398 274 147 411 4518 Fragments arranged by size: 4518 1768 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4021 4051 1178 Size: 30 165 30 2618 30 4643 Fragments arranged by size: 4643 2618 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3668 3990 4299 1186 Size: 2482 322 309 4403 Fragments arranged by size: 4403 2482 322 309 BseYI C'CCAG_C Cuts at: 4301 6719 7215 4301 Size: 2418 496 4602 Fragments arranged by size: 4602 2418 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2918 3464 1163 Size: 573 1182 546 5215 Fragments arranged by size: 5215 1182 573 546 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4966 5727 6755 1540 Size: 275 186 2965 761 1028 2301 Fragments arranged by size: 2965 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3803 4765 5577 5662 6733 3803 Size: 962 812 85 1071 4586 Fragments arranged by size: 4586 1071 962 812 85 BsmI GAATG_Cn' Cuts at: 2648 5027 5120 2648 Size: 2379 93 5044 Fragments arranged by size: 5044 2379 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3650 3650 Size: 7516 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3803 4209 4382 4765 4835 5577 5662 Size: 399 2013 406 173 383 70 742 85 Cuts at: 5662 6733 1391 Size: 1071 2174 Fragments arranged by size: 2174 2013 1071 742 406 399 383 173 85 70 BspEI T'CCGG_A Cuts at: 4181 7179 4181 Size: 2998 4518 BspHI T'CATG_A Cuts at: 3027 4386 5405 7135 3027 Size: 1359 1019 1730 3408 Fragments arranged by size: 3408 1730 1359 1019 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3508 3800 4060 4159 4871 844 Size: 2664 292 260 99 712 3489 Fragments arranged by size: 3489 2664 712 292 260 99 BsrBI CCG'CTC Cuts at: 1394 1823 2380 2731 5403 1394 Size: 429 557 351 2672 3507 Fragments arranged by size: 3507 2672 557 429 351 BsrDI GCAATG_nn' Cuts at: 65 1248 3054 3185 6011 6185 65 Size: 1183 1806 131 2826 174 1396 Fragments arranged by size: 2826 1806 1396 1183 174 131 BsrFI r'CCGG_y Cuts at: 1700 1810 6160 6347 1700 Size: 110 4350 187 2869 Fragments arranged by size: 4350 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7516 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7516 BssSI C'ACGA_G Cuts at: 5576 6588 5576 Size: 1012 6504 BstAPI GCAn_nnn'nTGC Cuts at: 794 4034 4660 794 Size: 3240 626 3650 Fragments arranged by size: 3650 3240 626 BstBI TT'CG_AA Cuts at: 3115 6320 3115 Size: 3205 4311 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7516 BstXI CCAn_nnnn'nTGG Cuts at: 3154 3952 3154 Size: 798 6718 BstYI r'GATC_y Cuts at: 1972 2293 2404 2503 3892 3964 4846 5213 5598 Size: 321 111 99 1389 72 882 367 385 Cuts at: 5598 5615 7067 7153 7165 7511 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1389 882 385 367 346 321 111 99 86 72 17 12 Bsu36I CC'TnA_GG Cuts at: 2817 2912 3858 4134 2817 Size: 95 946 276 6199 Fragments arranged by size: 6199 946 276 95 BtgI C'CryG_G Cuts at: 513 1065 2130 2331 513 Size: 552 1065 201 5698 Fragments arranged by size: 5698 1065 552 201 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3274 487 Size: 683 56 2048 4729 Fragments arranged by size: 4729 2048 683 56 BtsI GCAGTG_nn' Cuts at: 2772 3834 3966 4072 5032 5826 5846 2772 Size: 1062 132 106 960 794 20 4442 Fragments arranged by size: 4442 1062 960 794 132 106 20 ClaI AT'CG_AT Cuts at: 1554 4437 1554 Size: 2883 4633 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2514 2549 529 Size: 35 1950 35 5496 Fragments arranged by size: 5496 1950 35 35 DraI TTT'AAA Cuts at: 3900 4581 4820 3900 Size: 681 239 6596 Fragments arranged by size: 6596 681 239 DraIII CAC_nnn'GTG Cuts at: 3300 4118 3300 Size: 818 6698 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3987 6523 809 Size: 507 2671 2536 1802 Fragments arranged by size: 2671 2536 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2253 4314 4704 Size: 54 1425 50 275 18 423 2061 390 Cuts at: 4704 4963 5852 7481 8 Size: 259 889 1629 43 Fragments arranged by size: 2061 1629 1425 889 423 390 275 259 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4963 1537 Size: 275 3151 4090 Fragments arranged by size: 4090 3151 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5446 1353 Size: 4093 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6103 6477 6623 387 Size: 1064 138 4514 374 146 1280 Fragments arranged by size: 4514 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4763 4763 Size: 7516 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2867 3434 4397 4435 4538 4697 4772 Size: 393 1235 567 963 38 103 159 75 Cuts at: 4772 4788 5579 6176 6963 7231 1239 Size: 16 791 597 787 268 1524 Fragments arranged by size: 1524 1235 963 791 787 597 567 393 268 159 103 75 38 16 EcoRI G'AATT_C Cuts at: 3765 4825 5203 3765 Size: 1060 378 6078 Fragments arranged by size: 6078 1060 378 EcoRV GAT'ATC Cuts at: 2455 2455 Size: 7516 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3899 3899 Size: 7516 FspI TGC'GCA Cuts at: 6022 6022 Size: 7516 HaeII r_GCGC'y Cuts at: 1678 3175 3589 4507 6663 1678 Size: 1497 414 918 2156 2531 Fragments arranged by size: 2531 2156 1497 918 414 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2173 2205 2666 2698 4384 Size: 32 135 32 601 32 461 32 1686 Cuts at: 4384 4416 6157 6189 6231 6263 1373 Size: 32 1741 32 42 32 2626 Fragments arranged by size: 2626 1741 1686 601 461 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3106 4860 5106 669 Size: 988 281 1168 1754 246 3079 Fragments arranged by size: 3079 1754 1168 988 281 246 HindIII A'AGCT_T Cuts at: 748 2204 3406 748 Size: 1456 1202 4858 Fragments arranged by size: 4858 1456 1202 HpaI GTT'AAC Cuts at: 5106 5106 Size: 7516 KasI G'GCGC_C Cuts at: 3585 3585 Size: 7516 KpnI G_GTAC'C Cuts at: 4841 4841 Size: 7516 MfeI C'AATT_G Cuts at: 3234 5115 3234 Size: 1881 5635 MluI A'CGCG_T Cuts at: 7392 7392 Size: 7516 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3000 6630 6814 1305 Size: 1695 3630 184 2007 Fragments arranged by size: 3630 2007 1695 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2255 4316 10 Size: 54 1425 343 423 2061 3210 Fragments arranged by size: 3210 2061 1425 423 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3152 3440 3950 4214 4391 4581 4753 Size: 648 1986 288 510 264 177 190 172 Cuts at: 4753 5474 5833 5992 518 Size: 721 359 159 2042 Fragments arranged by size: 2042 1986 721 648 510 359 288 264 190 177 172 159 MspA1I CmG'CkG Cuts at: 1606 2780 4455 4932 5609 6757 7002 1606 Size: 1174 1675 477 677 1148 245 2120 Fragments arranged by size: 2120 1675 1174 1148 677 477 245 NarI GG'CG_CC Cuts at: 3586 3586 Size: 7516 NcoI C'CATG_G Cuts at: 513 1065 2331 513 Size: 552 1266 5698 Fragments arranged by size: 5698 1266 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7516 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7516 NotI GC'GGCC_GC Cuts at: 4963 4963 Size: 7516 NspI r_CATG'y Cuts at: 1132 1229 4663 4874 6419 1132 Size: 97 3434 211 1545 2229 Fragments arranged by size: 3434 2229 1545 211 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7516 PciI A'CATG_T Cuts at: 6415 6415 Size: 7516 PflMI CCAn_nnn'nTGG Cuts at: 1108 2470 1108 Size: 1362 6154 PfoI T'CCnGG_A Cuts at: 2670 3411 2670 Size: 741 6775 PmeI GTTT'AAAC Cuts at: 4820 4820 Size: 7516 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3299 3331 4177 4209 5585 5617 7098 7130 3299 Size: 32 846 32 1376 32 1481 32 3685 Fragments arranged by size: 3685 1481 1376 846 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3912 1613 Size: 2299 5217 PsiI TTA'TAA Cuts at: 5086 5086 Size: 7516 PspOMI G'GGCC_C Cuts at: 4378 4378 Size: 7516 PspXI vC'TCGA_Gb Cuts at: 3336 3336 Size: 7516 PstI C_TGCA'G Cuts at: 830 3169 4156 4248 4868 830 Size: 2339 987 92 620 3478 Fragments arranged by size: 3478 2339 987 620 92 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7516 PvuII CAG'CTG Cuts at: 4455 4455 Size: 7516 SacI G_AGCT'C Cuts at: 4765 4765 Size: 7516 SalI G'TCGA_C Cuts at: 1936 4858 1936 Size: 2922 4594 SbfI CC_TGCA'GG Cuts at: 4868 4868 Size: 7516 ScaI AGT'ACT Cuts at: 5764 7476 5764 Size: 1712 5804 SexAI A'CCwGG_T Cuts at: 4114 4114 Size: 7516 SfcI C'TryA_G Cuts at: 826 1045 3090 3165 4152 4244 4864 5999 6680 Size: 219 2045 75 987 92 620 1135 681 Cuts at: 6680 6871 826 Size: 191 1471 Fragments arranged by size: 2045 1471 1135 987 681 620 219 191 92 75 SfoI GGC'GCC Cuts at: 3587 3587 Size: 7516 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7516 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7516 SmaI CCC'GGG Cuts at: 1872 4843 1872 Size: 2971 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3045 3336 3482 3523 3873 5620 Size: 197 926 1102 291 146 41 350 1747 Cuts at: 5620 6521 6783 820 Size: 901 262 1553 Fragments arranged by size: 1747 1553 1102 926 901 350 291 262 197 146 41 SnaBI TAC'GTA Cuts at: 493 4715 5307 493 Size: 4222 592 2702 Fragments arranged by size: 4222 2702 592 SpeI A'CTAG_T Cuts at: 152 152 Size: 7516 SphI G_CATG'C Cuts at: 1132 4874 1132 Size: 3742 3774 SspI AAT'ATT Cuts at: 5 52 2049 5440 5 Size: 47 1997 3391 2081 Fragments arranged by size: 3391 2081 1997 47 StuI AGG'CCT Cuts at: 1517 2944 1517 Size: 1427 6089 StyI C'CwwG_G Cuts at: 513 1065 2316 2331 3970 4201 4374 4955 513 Size: 552 1251 15 1639 231 173 581 3074 Fragments arranged by size: 3074 1639 1251 581 552 231 173 15 SwaI ATTT'AAAT Cuts at: 4581 4581 Size: 7516 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2592 3452 3902 4009 5543 5560 5713 Size: 1255 1012 860 450 107 1534 17 153 Cuts at: 5713 5898 6343 325 Size: 185 445 1498 Fragments arranged by size: 1534 1498 1255 1012 860 450 445 185 153 107 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2985 5762 7474 Size: 274 80 33 51 1443 1008 2777 1712 Cuts at: 7474 96 Size: 138 Fragments arranged by size: 2777 1712 1443 1008 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7476 7508 7476 Size: 32 7484 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7516 XbaI T'CTAG_A Cuts at: 2803 4852 2803 Size: 2049 5467 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2764 3745 4309 4642 2764 Size: 981 564 333 5638 Fragments arranged by size: 5638 981 564 333 XhoI C'TCGA_G Cuts at: 1943 3336 1943 Size: 1393 6123 XmaI C'CCGG_G Cuts at: 1870 4841 1870 Size: 2971 4545 XmnI GAAnn'nnTTC Cuts at: 5645 5645 Size: 7516 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5324 276 Size: 53 83 186 936 3790 2468 Fragments arranged by size: 3790 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BbvCI BfrBI BplI BsiWI BstZ17I EcoNI EcoO109I FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PsrI RsrII SacII SanDI SapI SfiI SrfI