Restriction map for
pFN21AB4173
##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
Acc65I AccIII AclI AcsI AflII
AgeI AocI Aor51HI ApoI AscI
AseI AsnI Asp700I Asp718I AspI
AviII AvrII BbrPI BbsI BbvCI
BcgI BclI BfrI BglII BlnI
BpuAI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HindIII HpaI KpnI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
RcaI RsrII SapI ScaI SfuI
SgrAI SmlI SnaBI SpeI SphI
SspBI SspI SwaI TfiI Tsp509I
Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AhdI 907 GACGGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 664 GTGCAC G^TGCAC
ApaLI 664 GTGCAC G^TGCAC
AspEI 907 GACGGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 1075 GGATAC GTATCC(6/5)
BfaI 2204 CTAG C^TAG
BlpI 804 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 804 GCTCAGC GC^T[ACGT]AGC
BsrBI 1797 CCGCTC CCGCTC(-3/-3)
BsrDI 944 GCAATG GCAATG(2/0)
BssSI 52 CTCGTG CACGAG(-5/-1)
CelII 804 GCTCAGC GC^T[ACGT]AGC
Eam1105I 907 GACGGCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 2179 CTCTTC CTCTTC(1/4)
HgaI 2415 GACGC GACGC(5/10)
HincII 993 GTCGAC GT[CT]^[GA]AC
HindII 993 GTCGAC GT[CT]^[GA]AC
Ksp632I 2179 CTCTTC CTCTTC(1/4)
KspI 315 CCGCGG CCGC^GG
MaeI 2204 CTAG C^TAG
MseI 1901 TTAA T^TAA
NspI 1541 GCATGT [GA]CATG^[CT]
PflMI 1155 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1375 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 2651 CGATCG CGAT^CG
SacII 315 CCGCGG CCGC^GG
SalI 993 GTCGAC G^TCGAC
SbfI 2475 CCTGCAGG CCTGCA^GG
SexAI 1523 ACCAGGT A^CC[AT]GGT
Sse8387I 2475 CCTGCAGG CCTGCA^GG
StuI 774 AGGCCT AGG^CCT
Tru9I 1901 TTAA T^TAA
Van91I 1155 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AccI 993 GTCGAC GT^[AC][GT]AC
1015 GTCTAC
AflIII 1847 ACGTGT A^C[GA][CT]GT
2670 ACGTGT
BamHI 1004 GGATCC G^GATCC
1326 GGATCC
BbeI 708 GGCGCC GGCGC^C
2733 GGCGCC
BsaAI 1627 TACGTG [CT]AC^GT[GA]
2669 TACGTG
DrdI 2791 GACACCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2806 GACATCTGTGTC
EcoNI 1745 CCTCCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2538 CCTGACCAAGG
KasI 708 GGCGCC G^GCGCC
2733 GGCGCC
NarI 708 GGCGCC GG^CGCC
2733 GGCGCC
SacI 423 GAGCTC GAGCT^C
1854 GAGCTC
SfaNI 199 GATGC GCATC(5/9)
801 GATGC
SfiI 2070 GGCCCAGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2271 GGCCGGGAAGGCC
SfoI 708 GGCGCC GGC^GCC
2733 GGCGCC
SmaI 194 CCCGGG CCC^GGG
1444 CCCGGG
XmaCI 194 CCCGGG C^CCGGG
1444 CCCGGG
XmaI 194 CCCGGG C^CCGGG
1444 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AatII AccII AciI AcyI AfaI
AluI AlwI AlwNI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EagI EclXI
Eco52I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI StyI TaqI TseI
Tsp45I TspRI TthHB8I XcmI XhoII