(Circular) (Six-base) MAPSORT of: pFN21AB4173.seq Check: 4095 from: 1 to: 7561 pFN21AB4173 7561 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4381 4381 Size: 7561 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2256 3204 3942 5371 Size: 53 83 186 936 720 948 738 1429 Cuts at: 5371 278 Size: 2468 Fragments arranged by size: 2468 1429 948 936 738 720 186 83 53 AccI GT'mk_AC Cuts at: 1937 2999 3021 4904 1937 Size: 1062 22 1883 4594 Fragments arranged by size: 4594 1883 1062 22 Acc65I G'GTAC_C Cuts at: 4882 4882 Size: 7561 AclI AA'CG_TT Cuts at: 1563 5688 6061 1563 Size: 4125 373 3063 Fragments arranged by size: 4125 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2726 4048 5624 7008 7276 1632 Size: 1094 1322 1576 1384 268 1917 Fragments arranged by size: 1917 1576 1384 1322 1094 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7364 AflIII A'CryG_T Cuts at: 1203 3852 4675 6460 7437 1203 Size: 2649 823 1785 977 1327 Fragments arranged by size: 2649 1785 1327 977 823 AgeI A'CCGG_T Cuts at: 6392 6392 Size: 7561 AhdI GACnn_n'nnGTC Cuts at: 2917 6290 2917 Size: 3373 4188 AleI CACnn'nnGTG Cuts at: 1166 2708 4265 4658 1166 Size: 1542 1557 393 4069 Fragments arranged by size: 4069 1557 1542 393 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7561 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2414 2446 3233 3265 3554 3586 2414 Size: 32 787 32 289 32 6389 Fragments arranged by size: 6389 787 289 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2353 3067 3295 3430 4237 5304 6876 2353 Size: 714 228 135 807 1067 1572 3038 Fragments arranged by size: 3038 1572 1067 807 714 228 135 ApaI G_GGCC'C Cuts at: 2199 3038 3187 3356 4490 2199 Size: 839 149 169 1134 5270 Fragments arranged by size: 5270 1134 839 169 149 ApaLI G'TGCA_C Cuts at: 2669 5618 6774 2669 Size: 2949 1156 3456 Fragments arranged by size: 3456 2949 1156 ApoI r'AATT_y Cuts at: 1493 4870 5096 5248 7357 1493 Size: 3377 226 152 2109 1697 Fragments arranged by size: 3377 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6115 160 Size: 872 5083 1606 Fragments arranged by size: 5083 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7561 AvaI C'yCGr_G Cuts at: 1870 1943 2121 2199 3349 3449 3774 4563 4886 Size: 73 178 78 1150 100 325 789 323 Cuts at: 4886 1870 Size: 4545 Fragments arranged by size: 4545 1150 789 325 323 178 100 78 73 BamHI G'GATC_C Cuts at: 3009 3331 4891 5258 3009 Size: 322 1560 367 5312 Fragments arranged by size: 5312 1560 367 322 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2016 2581 2614 2713 3532 Size: 325 217 627 229 565 33 99 819 Cuts at: 3532 3919 4336 4738 4882 6337 618 Size: 387 417 402 144 1455 1842 Fragments arranged by size: 1842 1455 819 627 565 417 402 387 325 229 217 144 99 33 BanII G_rGCy'C Cuts at: 1391 2199 2365 2432 2655 2981 3038 3045 3187 Size: 808 166 67 223 326 57 7 142 Cuts at: 3187 3356 3590 3863 4490 4880 1391 Size: 169 234 273 627 390 4072 Fragments arranged by size: 4072 808 627 390 326 273 234 223 169 166 142 67 57 7 BbeI G_GCGC'C Cuts at: 2717 4742 2717 Size: 2025 5536 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6590 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5752 5786 1903 Size: 34 3815 34 3678 Fragments arranged by size: 3815 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3074 5453 6663 3074 Size: 2379 1210 3972 Fragments arranged by size: 3972 2379 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7561 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3132 4082 4283 Size: 107 122 71 631 605 1460 950 201 Cuts at: 4283 6172 136 Size: 1889 1525 Fragments arranged by size: 1889 1525 1460 950 631 605 201 122 107 71 BglII A'GATC_T Cuts at: 7556 7556 Size: 7561 BlpI GC'TnA_GC Cuts at: 2810 4978 2810 Size: 2168 5393 Bme1580I G_kGCm'C Cuts at: 1790 2091 2199 2241 2586 2617 2673 3038 3187 Size: 301 108 42 345 31 56 365 149 Cuts at: 3187 3356 3537 4316 4490 5622 6778 1790 Size: 169 181 779 174 1132 1156 2573 Fragments arranged by size: 2573 1156 1132 779 365 345 301 181 174 169 149 108 56 42 31 BmgBI CAC'GTC Cuts at: 1318 2515 3853 4156 1318 Size: 1197 1338 303 4723 Fragments arranged by size: 4723 1338 1197 303 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2138 3112 4259 Size: 416 53 83 399 24 721 974 1147 Cuts at: 4259 5292 6250 442 Size: 1033 958 1753 Fragments arranged by size: 1753 1147 1033 974 958 721 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7561 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2171 2438 2927 3013 3638 3644 3667 4211 Size: 932 267 489 86 625 6 23 544 Cuts at: 4211 4256 4628 6221 1239 Size: 45 372 1593 2579 Fragments arranged by size: 2579 1593 932 625 544 489 372 267 86 45 23 6 Bpu10I CC'TnA_GC Cuts at: 2426 2987 2426 Size: 561 7000 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5686 6551 6849 5686 Size: 865 298 6398 Fragments arranged by size: 6398 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2585 2889 3410 4160 6224 882 Size: 617 1086 304 521 750 2064 2219 Fragments arranged by size: 2219 2064 1086 750 617 521 304 BsaAI yAC'GTr Cuts at: 493 1204 3634 4676 5352 493 Size: 711 2430 1042 676 2702 Fragments arranged by size: 2702 2430 1042 711 676 BsaBI GATnn'nnATC Cuts at: 7555 7555 Size: 7561 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2253 2714 3201 3939 Size: 53 83 186 936 720 461 487 738 Cuts at: 3939 4739 5368 5750 6385 275 Size: 800 629 382 635 1451 Fragments arranged by size: 1451 936 800 738 720 635 629 487 461 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5994 6392 6666 6813 7224 5994 Size: 398 274 147 411 6331 Fragments arranged by size: 6331 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2414 2444 3215 3245 1178 Size: 30 165 30 1011 30 771 30 5494 Fragments arranged by size: 5494 1011 771 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2128 2168 2422 2663 3130 3871 3983 4109 Size: 942 40 254 241 467 741 112 126 Cuts at: 4109 1186 Size: 4638 Fragments arranged by size: 4638 942 741 467 254 241 126 112 40 BseYI C'CCAG_C Cuts at: 2164 2396 2405 3129 3913 3990 3994 4077 4463 Size: 232 9 724 784 77 4 83 386 Cuts at: 4463 4743 6764 7260 2164 Size: 280 2021 496 2465 Fragments arranged by size: 2465 2021 784 724 496 386 280 232 83 77 9 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2061 3077 3281 3308 3701 1163 Size: 573 325 1016 204 27 393 5023 Fragments arranged by size: 5023 1016 573 393 325 204 27 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2224 2689 3310 3826 4659 5011 Size: 275 186 223 465 621 516 833 352 Cuts at: 5011 5772 6800 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 833 761 621 516 465 352 275 223 186 BsiHKAI G_wGCw'C Cuts at: 2432 2673 2793 3863 4272 5622 5707 6778 2432 Size: 241 120 1070 409 1350 85 1071 3215 Fragments arranged by size: 3215 1350 1071 1070 409 241 120 85 BsmI GAATG_Cn' Cuts at: 5072 5165 5072 Size: 93 7468 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2199 2241 2365 2432 2586 2617 Size: 399 301 108 42 124 67 154 31 Cuts at: 2617 2655 2673 2793 2981 3038 3045 3187 3356 Size: 38 18 120 188 57 7 142 169 Cuts at: 3356 3537 3590 3863 4272 4316 4490 4880 5622 Size: 181 53 273 409 44 174 390 742 Cuts at: 5622 5707 6778 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 409 399 390 301 273 188 181 174 169 154 142 124 120 108 85 67 57 53 44 42 38 31 18 7 BspEI T'CCGG_A Cuts at: 7224 7224 Size: 7561 BspHI T'CATG_A Cuts at: 5450 7180 5450 Size: 1730 5831 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3723 3918 4335 4381 4738 4916 844 Size: 2879 195 417 46 357 178 3489 Fragments arranged by size: 3489 2879 417 357 195 178 46 BsrBI CCG'CTC Cuts at: 1394 1823 3804 5448 1394 Size: 429 1981 1644 3507 Fragments arranged by size: 3507 1981 1644 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2956 6056 6230 65 Size: 1183 1708 3100 174 1396 Fragments arranged by size: 3100 1708 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2034 2673 3309 3419 3833 4072 4426 Size: 110 224 639 636 110 414 239 354 Cuts at: 4426 6205 6392 1700 Size: 1779 187 2869 Fragments arranged by size: 2869 1779 639 636 414 354 239 224 187 110 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7561 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7561 BssSI C'ACGA_G Cuts at: 2057 5621 6633 2057 Size: 3564 1012 2985 Fragments arranged by size: 3564 2985 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2075 2353 3295 4336 794 Size: 1281 278 942 1041 4019 Fragments arranged by size: 4019 1281 1041 942 278 BstBI TT'CG_AA Cuts at: 6365 6365 Size: 7561 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7561 BstXI CCAn_nnnn'nTGG Cuts at: 2962 3052 3490 4531 4807 2962 Size: 90 438 1041 276 5716 Fragments arranged by size: 5716 1041 438 276 90 BstYI r'GATC_y Cuts at: 1972 2557 3009 3331 4891 5258 5643 5660 7112 Size: 585 452 322 1560 367 385 17 1452 Cuts at: 7112 7198 7210 7556 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1560 1452 585 452 385 367 346 322 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2320 2955 3282 3820 4170 4524 Size: 552 939 316 635 327 538 350 354 Cuts at: 4524 4734 513 Size: 210 3340 Fragments arranged by size: 3340 939 635 552 538 354 350 327 316 210 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3730 487 Size: 683 56 2504 4318 Fragments arranged by size: 4318 2504 683 56 BtsI GCAGTG_nn' Cuts at: 2241 3456 4418 5077 5871 5891 2241 Size: 1215 962 659 794 20 3911 Fragments arranged by size: 3911 1215 962 794 659 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7561 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7526 DraI TTT'AAA Cuts at: 4865 4865 Size: 7561 DraIII CAC_nnn'GTG Cuts at: 2247 2838 2932 2247 Size: 591 94 6876 Fragments arranged by size: 6876 591 94 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4802 4817 6568 809 Size: 507 3486 15 1751 1802 Fragments arranged by size: 3486 1802 1751 507 15 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2221 2451 Size: 54 1425 50 275 18 213 178 230 Cuts at: 2451 2686 3279 3307 3421 3666 3817 3823 3835 Size: 235 593 28 114 245 151 6 12 Cuts at: 3835 4641 4731 5008 5897 7526 8 Size: 806 90 277 889 1629 43 Fragments arranged by size: 1629 1425 889 806 593 277 275 245 235 230 213 178 151 114 90 54 50 43 28 18 12 6 EagI C'GGCC_G Cuts at: 1537 1812 2221 2686 3307 3823 5008 1537 Size: 275 409 465 621 516 1185 4090 Fragments arranged by size: 4090 1185 621 516 465 409 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4190 5491 1353 Size: 2837 1301 3423 Fragments arranged by size: 3423 2837 1301 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6148 6522 6668 387 Size: 1064 138 4559 374 146 1280 Fragments arranged by size: 4559 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2430 3861 2430 Size: 1431 6130 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2438 2726 2927 3013 3638 3644 Size: 393 539 267 288 201 86 625 6 Cuts at: 3644 3667 4048 4211 4256 4628 5624 6221 7008 Size: 23 381 163 45 372 996 597 787 Cuts at: 7008 7276 1239 Size: 268 1524 Fragments arranged by size: 1524 996 787 625 597 539 393 381 372 288 268 267 201 163 86 45 23 6 EcoNI CCTnn'n_nnAGG Cuts at: 3754 4547 3754 Size: 793 6768 EcoO109I rG'GnC_Cy Cuts at: 2195 2196 2848 3106 3183 3353 3372 3654 3999 Size: 1 652 258 77 170 19 282 345 Cuts at: 3999 4096 4249 4348 4486 4487 4617 4750 4804 Size: 97 153 99 138 1 130 133 54 Cuts at: 4804 2195 Size: 4952 Fragments arranged by size: 4952 652 345 282 258 170 153 138 133 130 99 97 77 54 19 1 1 EcoRI G'AATT_C Cuts at: 4870 5248 4870 Size: 378 7183 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3280 3280 Size: 7561 FspI TGC'GCA Cuts at: 6067 6067 Size: 7561 HaeII r_GCGC'y Cuts at: 1678 2575 2717 2906 3316 3764 4742 6708 1678 Size: 897 142 189 410 448 978 1966 2531 Fragments arranged by size: 2531 1966 978 897 448 410 189 142 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2542 2574 2903 2935 3316 Size: 32 135 32 970 32 329 32 381 Cuts at: 3316 3348 6202 6234 6276 6308 1373 Size: 32 2854 32 42 32 2626 Fragments arranged by size: 2854 2626 970 381 329 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3000 4905 5151 669 Size: 988 281 1062 1905 246 3079 Fragments arranged by size: 3079 1905 1062 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7561 HpaI GTT'AAC Cuts at: 5151 5151 Size: 7561 KasI G'GCGC_C Cuts at: 2713 4738 2713 Size: 2025 5536 KpnI G_GTAC'C Cuts at: 4886 4886 Size: 7561 MfeI C'AATT_G Cuts at: 5160 5160 Size: 7561 MluI A'CGCG_T Cuts at: 7437 7437 Size: 7561 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3597 4782 4800 6675 6859 1305 Size: 2292 1185 18 1875 184 2007 Fragments arranged by size: 2292 2007 1875 1185 184 18 MscI TGG'CCA Cuts at: 10 64 1489 1832 3819 4643 4733 10 Size: 54 1425 343 1987 824 90 2838 Fragments arranged by size: 2838 1987 1425 824 343 90 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2708 2960 4265 4658 5519 5878 Size: 648 843 699 252 1305 393 861 359 Cuts at: 5878 6037 518 Size: 159 2042 Fragments arranged by size: 2042 1305 861 843 699 648 393 359 252 159 MspA1I CmG'CkG Cuts at: 1606 2075 2322 2680 2746 2820 3129 3430 3994 Size: 469 247 358 66 74 309 301 564 Cuts at: 3994 4705 4854 4977 5654 6802 7047 1606 Size: 711 149 123 677 1148 245 2120 Fragments arranged by size: 2120 1148 711 677 564 469 358 309 301 247 245 149 123 74 66 NaeI GCC'GGC Cuts at: 3311 3835 4074 4428 3311 Size: 524 239 354 6444 Fragments arranged by size: 6444 524 354 239 NarI GG'CG_CC Cuts at: 2714 4739 2714 Size: 2025 5536 NcoI C'CATG_G Cuts at: 513 1065 2004 2955 3282 4524 513 Size: 552 939 951 327 1242 3550 Fragments arranged by size: 3550 1242 951 939 552 327 NdeI CA'TA_TG Cuts at: 387 387 Size: 7561 NgoMIV G'CCGG_C Cuts at: 3309 3833 4072 4426 3309 Size: 524 239 354 6444 Fragments arranged by size: 6444 524 354 239 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7561 NotI GC'GGCC_GC Cuts at: 5008 5008 Size: 7561 NspI r_CATG'y Cuts at: 1132 1229 3550 4919 6464 1132 Size: 97 2321 1369 1545 2229 Fragments arranged by size: 2321 2229 1545 1369 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7561 PciI A'CATG_T Cuts at: 6460 6460 Size: 7561 PflMI CCAn_nnn'nTGG Cuts at: 1108 3166 1108 Size: 2058 5503 PfoI T'CCnGG_A Cuts at: 2148 3012 3233 3267 4188 4806 2148 Size: 864 221 34 921 618 4903 Fragments arranged by size: 4903 921 864 618 221 34 PmeI GTTT'AAAC Cuts at: 4865 4865 Size: 7561 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2414 2446 5630 5662 7143 7175 2414 Size: 32 3184 32 1481 32 2800 Fragments arranged by size: 3184 2800 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2848 3654 4750 4804 2848 Size: 806 1096 54 5605 Fragments arranged by size: 5605 1096 806 54 PshAI GACnn'nnGTC Cuts at: 1613 3384 1613 Size: 1771 5790 PsiI TTA'TAA Cuts at: 5131 5131 Size: 7561 PspOMI G'GGCC_C Cuts at: 2195 3034 3183 3352 4486 2195 Size: 839 149 169 1134 5270 Fragments arranged by size: 5270 1134 839 169 149 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3614 3646 3614 Size: 32 7529 PstI C_TGCA'G Cuts at: 830 2358 2973 3903 4332 4378 4485 4704 4710 Size: 1528 615 930 429 46 107 219 6 Cuts at: 4710 4913 830 Size: 203 3478 Fragments arranged by size: 3478 1528 930 615 429 219 203 107 46 6 PvuI CG_AT'CG Cuts at: 2001 4659 2001 Size: 2658 4903 PvuII CAG'CTG Cuts at: 2680 2746 2820 3994 4705 4854 2680 Size: 66 74 1174 711 149 5387 Fragments arranged by size: 5387 1174 711 149 74 66 SacI G_AGCT'C Cuts at: 2432 3863 2432 Size: 1431 6130 SacII CC_GC'GG Cuts at: 2323 2323 Size: 7561 SalI G'TCGA_C Cuts at: 1936 2998 4903 1936 Size: 1062 1905 4594 Fragments arranged by size: 4594 1905 1062 SanDI GG'GwC_CC Cuts at: 3654 3654 Size: 7561 SbfI CC_TGCA'GG Cuts at: 4485 4913 4485 Size: 428 7133 ScaI AGT'ACT Cuts at: 5809 7521 5809 Size: 1712 5849 SexAI A'CCwGG_T Cuts at: 3528 3528 Size: 7561 SfcI C'TryA_G Cuts at: 826 1045 2354 2629 2969 3367 3899 4328 4374 Size: 219 1309 275 340 398 532 429 46 Cuts at: 4374 4481 4700 4706 4786 4909 6044 6725 6916 Size: 107 219 6 80 123 1135 681 191 Cuts at: 6916 826 Size: 1471 Fragments arranged by size: 1471 1309 1135 681 532 429 398 340 275 219 219 191 123 107 80 46 6 SfiI GGCCn_nnn'nGGCC Cuts at: 4082 4283 4082 Size: 201 7360 SfoI GGC'GCC Cuts at: 2715 4740 2715 Size: 2025 5536 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7561 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7561 SmaI CCC'GGG Cuts at: 1872 2201 3451 4888 1872 Size: 329 1250 1437 4545 Fragments arranged by size: 4545 1437 1250 329 SmlI C'TyrA_G Cuts at: 820 1017 1943 5665 6566 6828 820 Size: 197 926 3722 901 262 1553 Fragments arranged by size: 3722 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5352 493 Size: 4859 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7561 SphI G_CATG'C Cuts at: 1132 4919 1132 Size: 3787 3774 SspI AAT'ATT Cuts at: 5 52 5485 5 Size: 47 5433 2081 Fragments arranged by size: 5433 2081 47 StuI AGG'CCT Cuts at: 1517 2781 1517 Size: 1264 6297 StyI C'CwwG_G Cuts at: 513 1065 2004 2064 2487 2586 2955 3282 4200 Size: 552 939 60 423 99 369 327 918 Cuts at: 4200 4308 4351 4524 4548 4800 5000 513 Size: 108 43 173 24 252 200 3074 Fragments arranged by size: 3074 939 918 552 423 369 327 252 200 173 108 99 60 43 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2178 2315 2410 3752 4405 5588 5605 Size: 1255 598 137 95 1342 653 1183 17 Cuts at: 5605 5758 5943 6388 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1342 1255 1183 653 598 445 185 153 137 95 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5807 7519 96 Size: 274 80 33 51 1443 3830 1712 138 Fragments arranged by size: 3830 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7521 7553 7521 Size: 32 7529 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7561 XbaI T'CTAG_A Cuts at: 4897 4897 Size: 7561 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2413 3431 3613 4460 2413 Size: 1018 182 847 5514 Fragments arranged by size: 5514 1018 847 182 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7561 XmaI C'CCGG_G Cuts at: 1870 2199 3449 4886 1870 Size: 329 1250 1437 4545 Fragments arranged by size: 4545 1437 1250 329 XmnI GAAnn'nnTTC Cuts at: 5690 5690 Size: 7561 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2254 3202 3940 5369 Size: 53 83 186 936 720 948 738 1429 Cuts at: 5369 276 Size: 2468 Fragments arranged by size: 2468 1429 948 936 738 720 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI RsrII SapI SrfI SwaI