Restriction map for
pFN21AB4173
(Linear) (Six-base) MAPSORT of: KIBB4173.seq Check: 8614 from: 1 to: 2856
KIBB4173.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2376 2856
Size: 2376 480
AatII G_ACGT'C
Cuts at: 0 251 1199 1937 2856
Size: 251 948 738 919
Fragments arranged by size:
948 919 738 251
AccI GT'mk_AC
Cuts at: 0 994 1016 2856
Size: 994 22 1840
Fragments arranged by size:
1840 994 22
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 2043 2856
Size: 721 1322 813
Fragments arranged by size:
1322 813 721
AflIII A'CryG_T
Cuts at: 0 1847 2670 2856
Size: 1847 823 186
Fragments arranged by size:
1847 823 186
AhdI GACnn_n'nnGTC
Cuts at: 0 912 2856
Size: 912 1944
AleI CACnn'nnGTG
Cuts at: 0 703 2260 2653 2856
Size: 703 1557 393 203
Fragments arranged by size:
1557 703 393 203
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 409 441 1228 1260 1549 1581 2856
Size: 409 32 787 32 289 32 1275
Fragments arranged by size:
1275 787 409 289 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 348 1062 1290 1425 2232 2856
Size: 348 714 228 135 807 624
Fragments arranged by size:
807 714 624 348 228 135
ApaI G_GGCC'C
Cuts at: 0 194 1033 1182 1351 2485 2856
Size: 194 839 149 169 1134 371
Fragments arranged by size:
1134 839 371 194 169 149
ApaLI G'TGCA_C
Cuts at: 0 664 2856
Size: 664 2192
AvaI C'yCGr_G
Cuts at: 0 116 194 1344 1444 1769 2558 2856
Size: 116 78 1150 100 325 789 298
Fragments arranged by size:
1150 789 325 298 116 100 78
BamHI G'GATC_C
Cuts at: 0 1004 1326 2856
Size: 1004 322 1530
Fragments arranged by size:
1530 1004 322
BanI G'GyrC_C
Cuts at: 0 11 576 609 708 1527 1914 2331 2733
Size: 11 565 33 99 819 387 417 402
Cuts at: 2733 2856
Size: 123
Fragments arranged by size:
819 565 417 402 387 123 99 33
11
BanII G_rGCy'C
Cuts at: 0 194 360 427 650 976 1033 1040 1182
Size: 194 166 67 223 326 57 7 142
Cuts at: 1182 1351 1585 1858 2485 2856
Size: 169 234 273 627 371
Fragments arranged by size:
627 371 326 273 234 223 194 169
166 142 67 57 7
BbeI G_GCGC'C
Cuts at: 0 712 2737 2856
Size: 712 2025 119
Fragments arranged by size:
2025 712 119
BciVI GTATCCnnnnn_n'
Cuts at: 0 1069 2856
Size: 1069 1787
BglI GCCn_nnn'nGGC
Cuts at: 0 1127 2077 2278 2856
Size: 1127 950 201 578
Fragments arranged by size:
1127 950 578 201
BlpI GC'TnA_GC
Cuts at: 0 805 2856
Size: 805 2051
Bme1580I G_kGCm'C
Cuts at: 0 86 194 236 581 612 668 1033 1182
Size: 86 108 42 345 31 56 365 149
Cuts at: 1182 1351 1532 2311 2485 2856
Size: 169 181 779 174 371
Fragments arranged by size:
779 371 365 345 181 174 169 149
108 86 56 42 31
BmgBI CAC'GTC
Cuts at: 0 510 1848 2151 2856
Size: 510 1338 303 705
Fragments arranged by size:
1338 705 510 303
BmrI ACTGGGnnnn_n'
Cuts at: 0 133 1107 2254 2856
Size: 133 974 1147 602
Fragments arranged by size:
1147 974 602 133
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 433 922 1008 1633 1639 1662 2206
Size: 166 267 489 86 625 6 23 544
Cuts at: 2206 2251 2623 2856
Size: 45 372 233
Fragments arranged by size:
625 544 489 372 267 233 166 86
45 23 6
Bpu10I CC'TnA_GC
Cuts at: 0 421 982 2856
Size: 421 561 1874
Fragments arranged by size:
1874 561 421
BsaI GGTCTCn'nnnn_
Cuts at: 0 580 884 1405 2155 2856
Size: 580 304 521 750 701
Fragments arranged by size:
750 701 580 521 304
BsaAI yAC'GTr
Cuts at: 0 1629 2671 2856
Size: 1629 1042 185
Fragments arranged by size:
1629 1042 185
BsaHI Gr'CG_yC
Cuts at: 0 248 709 1196 1934 2734 2856
Size: 248 461 487 738 800 122
Fragments arranged by size:
800 738 487 461 248 122
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 409 439 1210 1240 2856
Size: 409 30 771 30 1616
Fragments arranged by size:
1616 771 409 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 123 163 417 658 1125 1866 1978 2104
Size: 123 40 254 241 467 741 112 126
Cuts at: 2104 2856
Size: 752
Fragments arranged by size:
752 741 467 254 241 126 123 112
40
BseYI C'CCAG_C
Cuts at: 0 159 391 400 1124 1908 1985 1989 2072
Size: 159 232 9 724 784 77 4 83
Cuts at: 2072 2458 2738 2856
Size: 386 280 118
Fragments arranged by size:
784 724 386 280 232 159 118 83
77 9 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 1072 1276 1303 1696 2856
Size: 56 1016 204 27 393 1160
Fragments arranged by size:
1160 1016 393 204 56 27
BsiEI CG_ry'CG
Cuts at: 0 219 684 1305 1821 2654 2856
Size: 219 465 621 516 833 202
Fragments arranged by size:
833 621 516 465 219 202
BsiHKAI G_wGCw'C
Cuts at: 0 427 668 788 1858 2267 2856
Size: 427 241 120 1070 409 589
Fragments arranged by size:
1070 589 427 409 241 120
Bsp1286I G_dGCh'C
Cuts at: 0 86 194 236 360 427 581 612 650
Size: 86 108 42 124 67 154 31 38
Cuts at: 650 668 788 976 1033 1040 1182 1351 1532
Size: 18 120 188 57 7 142 169 181
Cuts at: 1532 1585 1858 2267 2311 2485 2856
Size: 53 273 409 44 174 371
Fragments arranged by size:
409 371 273 188 181 174 169 154
142 124 120 108 86 67 57 53
44 42 38 31 18 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1718 1913 2330 2376 2733 2856
Size: 1718 195 417 46 357 123
Fragments arranged by size:
1718 417 357 195 123 46
BsrBI CCG'CTC
Cuts at: 0 1799 2856
Size: 1799 1057
BsrDI GCAATG_nn'
Cuts at: 0 951 2856
Size: 951 1905
BsrFI r'CCGG_y
Cuts at: 0 29 668 1304 1414 1828 2067 2421 2856
Size: 29 639 636 110 414 239 354 435
Fragments arranged by size:
639 636 435 414 354 239 110 29
BssSI C'ACGA_G
Cuts at: 0 52 2856
Size: 52 2804
BstAPI GCAn_nnn'nTGC
Cuts at: 0 70 348 1290 2331 2856
Size: 70 278 942 1041 525
Fragments arranged by size:
1041 942 525 278 70
BstXI CCAn_nnnn'nTGG
Cuts at: 0 957 1047 1485 2526 2802 2856
Size: 957 90 438 1041 276 54
Fragments arranged by size:
1041 957 438 276 90 54
BstYI r'GATC_y
Cuts at: 0 552 1004 1326 2856
Size: 552 452 322 1530
Fragments arranged by size:
1530 552 452 322
BtgI C'CryG_G
Cuts at: 0 315 950 1277 1815 2165 2519 2729 2856
Size: 315 635 327 538 350 354 210 127
Fragments arranged by size:
635 538 354 350 327 315 210 127
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1725 2856
Size: 1725 1131
BtsI GCAGTG_nn'
Cuts at: 0 236 1451 2413 2856
Size: 236 1215 962 443
Fragments arranged by size:
1215 962 443 236
DraIII CAC_nnn'GTG
Cuts at: 0 242 833 927 2856
Size: 242 591 94 1929
Fragments arranged by size:
1929 591 242 94
DrdI GACnn_nn'nnGTC
Cuts at: 0 2797 2812 2856
Size: 2797 15 44
Fragments arranged by size:
2797 44 15
EaeI y'GGCC_r
Cuts at: 0 38 216 446 681 1274 1302 1416 1661
Size: 38 178 230 235 593 28 114 245
Cuts at: 1661 1812 1818 1830 2636 2726 2856
Size: 151 6 12 806 90 130
Fragments arranged by size:
806 593 245 235 230 178 151 130
114 90 38 28 12 6
EagI C'GGCC_G
Cuts at: 0 216 681 1302 1818 2856
Size: 216 465 621 516 1038
Fragments arranged by size:
1038 621 516 465 216
EarI CTCTTCn'nnn_
Cuts at: 0 2185 2856
Size: 2185 671
EcoICRI GAG'CTC
Cuts at: 0 425 1856 2856
Size: 425 1431 1000
Fragments arranged by size:
1431 1000 425
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 433 721 922 1008 1633 1639 1662
Size: 166 267 288 201 86 625 6 23
Cuts at: 1662 2043 2206 2251 2623 2856
Size: 381 163 45 372 233
Fragments arranged by size:
625 381 372 288 267 233 201 166
163 86 45 23 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1749 2542 2856
Size: 1749 793 314
Fragments arranged by size:
1749 793 314
EcoO109I rG'GnC_Cy
Cuts at: 0 190 191 843 1101 1178 1348 1367 1649
Size: 190 1 652 258 77 170 19 282
Cuts at: 1649 1994 2091 2244 2343 2481 2482 2612 2745
Size: 345 97 153 99 138 1 130 133
Cuts at: 2745 2799 2856
Size: 54 57
Fragments arranged by size:
652 345 282 258 190 170 153 138
133 130 99 97 77 57 54 19
1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1275 2856
Size: 1275 1581
HaeII r_GCGC'y
Cuts at: 0 570 712 901 1311 1759 2737 2856
Size: 570 142 189 410 448 978 119
Fragments arranged by size:
978 570 448 410 189 142 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 537 569 898 930 1311 1343 2856
Size: 537 32 329 32 381 32 1513
Fragments arranged by size:
1513 537 381 329 32 32 32
HincII GTy'rAC
Cuts at: 0 995 2856
Size: 995 1861
KasI G'GCGC_C
Cuts at: 0 708 2733 2856
Size: 708 2025 123
Fragments arranged by size:
2025 708 123
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1592 2777 2795 2856
Size: 1592 1185 18 61
Fragments arranged by size:
1592 1185 61 18
MscI TGG'CCA
Cuts at: 0 1814 2638 2728 2856
Size: 1814 824 90 128
Fragments arranged by size:
1814 824 128 90
MslI CAynn'nnrTG
Cuts at: 0 703 955 2260 2653 2856
Size: 703 252 1305 393 203
Fragments arranged by size:
1305 703 393 252 203
MspA1I CmG'CkG
Cuts at: 0 70 317 675 741 815 1124 1425 1989
Size: 70 247 358 66 74 309 301 564
Cuts at: 1989 2700 2849 2856
Size: 711 149 7
Fragments arranged by size:
711 564 358 309 301 247 149 74
70 66 7
NaeI GCC'GGC
Cuts at: 0 1306 1830 2069 2423 2856
Size: 1306 524 239 354 433
Fragments arranged by size:
1306 524 433 354 239
NarI GG'CG_CC
Cuts at: 0 709 2734 2856
Size: 709 2025 122
Fragments arranged by size:
2025 709 122
NcoI C'CATG_G
Cuts at: 0 950 1277 2519 2856
Size: 950 327 1242 337
Fragments arranged by size:
1242 950 337 327
NgoMIV G'CCGG_C
Cuts at: 0 1304 1828 2067 2421 2856
Size: 1304 524 239 354 435
Fragments arranged by size:
1304 524 435 354 239
NspI r_CATG'y
Cuts at: 0 1545 2856
Size: 1545 1311
PflMI CCAn_nnn'nTGG
Cuts at: 0 1161 2856
Size: 1161 1695
PfoI T'CCnGG_A
Cuts at: 0 143 1007 1228 1262 2183 2801 2856
Size: 143 864 221 34 921 618 55
Fragments arranged by size:
921 864 618 221 143 55 34
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 409 441 2856
Size: 409 32 2415
Fragments arranged by size:
2415 409 32
PpuMI rG'GwC_Cy
Cuts at: 0 843 1649 2745 2799 2856
Size: 843 806 1096 54 57
Fragments arranged by size:
1096 843 806 57 54
PshAI GACnn'nnGTC
Cuts at: 0 1379 2856
Size: 1379 1477
PspOMI G'GGCC_C
Cuts at: 0 190 1029 1178 1347 2481 2856
Size: 190 839 149 169 1134 375
Fragments arranged by size:
1134 839 375 190 169 149
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1609 1641 2856
Size: 1609 32 1215
Fragments arranged by size:
1609 1215 32
PstI C_TGCA'G
Cuts at: 0 353 968 1898 2327 2373 2480 2699 2705
Size: 353 615 930 429 46 107 219 6
Cuts at: 2705 2856
Size: 151
Fragments arranged by size:
930 615 429 353 219 151 107 46
6
PvuI CG_AT'CG
Cuts at: 0 2654 2856
Size: 2654 202
PvuII CAG'CTG
Cuts at: 0 675 741 815 1989 2700 2849 2856
Size: 675 66 74 1174 711 149 7
Fragments arranged by size:
1174 711 675 149 74 66 7
SacI G_AGCT'C
Cuts at: 0 427 1858 2856
Size: 427 1431 998
Fragments arranged by size:
1431 998 427
SacII CC_GC'GG
Cuts at: 0 318 2856
Size: 318 2538
SalI G'TCGA_C
Cuts at: 0 993 2856
Size: 993 1863
SanDI GG'GwC_CC
Cuts at: 0 1649 2856
Size: 1649 1207
SbfI CC_TGCA'GG
Cuts at: 0 2480 2856
Size: 2480 376
SexAI A'CCwGG_T
Cuts at: 0 1523 2856
Size: 1523 1333
SfcI C'TryA_G
Cuts at: 0 349 624 964 1362 1894 2323 2369 2476
Size: 349 275 340 398 532 429 46 107
Cuts at: 2476 2695 2701 2781 2856
Size: 219 6 80 75
Fragments arranged by size:
532 429 398 349 340 275 219 107
80 75 46 6
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2077 2278 2856
Size: 2077 201 578
Fragments arranged by size:
2077 578 201
SfoI GGC'GCC
Cuts at: 0 710 2735 2856
Size: 710 2025 121
Fragments arranged by size:
2025 710 121
SmaI CCC'GGG
Cuts at: 0 196 1446 2856
Size: 196 1250 1410
Fragments arranged by size:
1410 1250 196
StuI AGG'CCT
Cuts at: 0 776 2856
Size: 776 2080
StyI C'CwwG_G
Cuts at: 0 59 482 581 950 1277 2195 2303 2346
Size: 59 423 99 369 327 918 108 43
Cuts at: 2346 2519 2543 2795 2856
Size: 173 24 252 61
Fragments arranged by size:
918 423 369 327 252 173 108 99
61 59 43 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 173 310 405 1747 2400 2856
Size: 173 137 95 1342 653 456
Fragments arranged by size:
1342 653 456 173 137 95
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 408 1426 1608 2455 2856
Size: 408 1018 182 847 401
Fragments arranged by size:
1018 847 408 401 182
XmaI C'CCGG_G
Cuts at: 0 194 1444 2856
Size: 194 1250 1412
Fragments arranged by size:
1412 1250 194
ZraI GAC'GTC
Cuts at: 0 249 1197 1935 2856
Size: 249 948 738 921
Fragments arranged by size:
948 921 738 249
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AhdI AleI AloI
AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI
BciVI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PflMI PfoI PpiI PpuMI
PshAI PspOMI PsrI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI SexAI SfcI SfiI SfoI SmaI
StuI StyI TaqII XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AlfI ApoI AscI
AseI AsiSI AvrII BaeI BbsI BbvCI BcgI BclI
BfrBI BglII BmtI BplI BpuEI BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BspHI BsrGI BssHII BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI EciI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KpnI MfeI MluI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PmeI PmlI PsiI PspXI RsrII SapI ScaI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI Tth111I XbaI XhoI XmnI