Restriction map for
pFN21AB4107
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AlwNI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BalI BbeI BbrPI BcgI BciVI
BclI BfrI BlnI BsaAI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Cfr10I ClaI CpoI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI SapI SbfI
SexAI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AflIII 698 ACATGT A^C[GA][CT]GT
Alw44I 865 GTGCAC G^TGCAC
ApaI 1354 GGGCCC GGGCC^C
ApaLI 865 GTGCAC G^TGCAC
Asp700I 268 GAACGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 530 CTCGGG C^[CT]CG[GA]G
BamHI 144 GGATCC G^GATCC
BbsI 694 GAAGAC GAAGAC(2/6)
BbvCI 509 CCTCAGC CCTCAGC(-5/-2)
BcnI 74 CCCGG CC^[GC]GG
BglI 1266 GCCTCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 391 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 391 GCTCAGC GC^T[ACGT]AGC
BpuAI 694 GAAGAC GAAGAC(2/6)
BsaI 1326 GAGACC GGTCTC(1/5)
BsoBI 530 CTCGGG C^[CT]CG[GA]G
BspLU11I 698 ACATGT A^CATGT
BssSI 1451 CTCGTG CACGAG(-5/-1)
BstXI 1134 CCATTCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 1170 GCAGGC GC[ACGT]^[ACGT]GC
CelII 391 GCTCAGC GC^T[ACGT]AGC
DraI 132 TTTAAA TTT^AAA
EaeI 645 TGGCCG [CT]^GGCC[GA]
EcoNI 1408 CCTCCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HapII 75 CCGG C^CGG
HpaII 75 CCGG C^CGG
HphI 880 GGTGA GGTGA(8/7)
MspI 75 CCGG C^CGG
NciI 74 CCCGG CC^[GC]GG
PstI 125 CTGCAG CTGCA^G
SacI 1310 GAGCTC GAGCT^C
SfiI 1265 GGCCTCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 626 CTCAAG C^T[CT][GA]AG
SspI 368 AATATT AAT^ATT
StuI 1284 AGGCCT AGG^CCT
XmnI 268 GAACGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AocI 775 CCTGAGG CC^T[ACGT]AGG
1027 CCTGAGG
AspHI 865 GTGCAC G[AT]GC[AT]^C
1310 GAGCTC
BanI 478 GGTGCC G^G[CT][GA]CC
1533 GGTGCC
BglII 10 AGATCT A^GATCT
669 AGATCT
BsaBI 11 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
766 GATGGCTATC
BsiHKAI 865 GTGCAC G[AT]GC[AT]^C
1310 GAGCTC
BsmI 387 GAATGC GAATGC(1/-1)
1337 GAATGC
BspHI 732 TCATGA T^CATGA
1898 TCATGA
BsrI 199 CCAGT ACTGG(1/-1)
755 CCAGT
Bsu36I 775 CCTGAGG CC^T[ACGT]AGG
1027 CCTGAGG
CfoI 108 GCGC GCG^C
1521 GCGC
DraII 1353 AGGGCCC [GA]G^G[ACGT]CC[CT]
1354 GGGCCCT
Eco81I 775 CCTGAGG CC^T[ACGT]AGG
1027 CCTGAGG
EcoO109I 1353 AGGGCCC [GA]G^G[ACGT]CC[CT]
1354 GGGCCCT
HhaI 108 GCGC GCG^C
1521 GCGC
HinP1I 108 GCGC G^CGC
1521 GCGC
MaeIII 821 GTGAC ^GT[ACGT]AC
848 GTGAC
MamI 11 GATCTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
766 GATGGCTATC
MslI 733 CATGATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1135 CATTCTGATG
RcaI 732 TCATGA T^CATGA
1898 TCATGA
ScaI 228 AGTACT AGT^ACT
808 AGTACT
SfcI 125 CTGCAG C^T[GA][CT]AG
1280 CTACAG
TaqI 120 TCGA T^CGA
1728 TCGA
Tsp45I 821 GTGAC ^GT[GC]AC
848 GTGAC
TthHB8I 120 TCGA T^CGA
1728 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanII BbvI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
ItaI Ksp632I MaeI MaeII MboI
MboII MflI MnlI MseI MvaI
MwoI NdeII NlaIII NlaIV NspI
PflMI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TfiI Tru9I
TseI Tsp509I TspRI Van91I XhoII