Restriction map for
pFN21AB4082
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AgeI Alw44I AlwNI ApaLI AscI
AseI AsnI Asp718I AspI AviII
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BglII BsaBI BsaI
BseAI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BssHII BstBI BstEII
BstPI BstUI Cfr10I CpoI DraI
DraIII DsaI EagI EarI EclXI
Eco52I EcoNI EcoO65I EcoRV FauI
FseI FspI HpaI KasI KpnI
Ksp632I KspI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 592 GACGTC GACGT^C
AcyI 592 GACGTC G[GA]^CG[CT]C
AhdI 316 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 969 CCTGAGG CC^T[ACGT]AGG
ApaI 353 GGGCCC GGGCC^C
Asp700I 198 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 316 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 705 CCTAGG C^CTAGG
BanI 1840 GGTGCC G^G[CT][GA]CC
BclI 1925 TGATCA T^GATCA
BlnI 705 CCTAGG C^CTAGG
BsaAI 220 CACGTA [CT]AC^GT[GA]
BsaHI 592 GACGTC G[GA]^CG[CT]C
BsaWI 2050 TCCGGT [AT]^CCGG[AT]
BseRI 972 GAGGAG GAGGAG(10/8)
BsmI 897 GAATGC GAATGC(1/-1)
Bsp1407I 1310 TGTACA T^GTACA
BspDI 1147 ATCGAT AT^CGAT
BspLU11I 1392 ACATGT A^CATGT
BspMI 1721 GCAGGT ACCTGC(4/8)
BsrGI 1310 TGTACA T^GTACA
BssSI 1769 CTCGTG CACGAG(-5/-1)
Bst1107I 912 GTATAC GTA^TAC
BstXI 2513 CCATGATGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 912 GTATAC GTA^TAC
Bsu36I 969 CCTGAGG CC^T[ACGT]AGG
ClaI 1147 ATCGAT AT^CGAT
DrdI 658 GACTGCGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 316 GACCAGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 969 CCTGAGG CC^T[ACGT]AGG
EcoT22I 781 ATGCAT ATGCA^T
FbaI 1925 TGATCA T^GATCA
Hin1I 592 GACGTC G[GA]^CG[CT]C
HindIII 956 AAGCTT A^AGCTT
MspA1I 2123 CAGCGG C[AC]G^C[GT]G
NsiI 781 ATGCAT ATGCA^T
PaeR7I 282 CTCGAG C^TCGAG
PpuMI 2038 GGGACCC [GA]G^G[AT]CC[CT]
SacI 1067 GAGCTC GAGCT^C
SspBI 1310 TGTACA T^GTACA
XhoI 282 CTCGAG C^TCGAG
XmnI 198 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 912 GTATAC GT^[AC][GT]AC
1366 GTAGAC
AflIII 1392 ACATGT A^C[GA][CT]GT
2654 ACGTGT
AvaI 282 CTCGAG C^[CT]CG[GA]G
2168 CCCGAG
BalI 2 TGGCCA TGG^CCA
2510 TGGCCA
BamHI 1449 GGATCC G^GATCC
1644 GGATCC
BanII 353 GGGCCC G[GA]GC[CT]^C
1067 GAGCTC
BlpI 1019 GCTTAGC GC^T[ACGT]AGC
2312 GCTCAGC
Bpu1102I 1019 GCTTAGC GC^T[ACGT]AGC
2312 GCTCAGC
BsiYI 344 CCTTCTGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1965 CCTGACTGTGG
BslI 344 CCTTCTGTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1965 CCTGACTGTGG
BsmAI 1013 GAGAC GTCTC(1/5)
1456 GAGAC
BsmFI 2038 GGGAC GGGAC(10/14)
993 GTCCC
BsoBI 282 CTCGAG C^[CT]CG[GA]G
2168 CCCGAG
BsrDI 1241 GCAATG GCAATG(2/0)
2465 GCAATG
BstAPI 24 GCAAAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1024 GCAAATCCTGC
CelII 1019 GCTTAGC GC^T[ACGT]AGC
2312 GCTCAGC
EaeI 2 TGGCCA [CT]^GGCC[GA]
2510 TGGCCA
HgaI 394 GACGC GACGC(5/10)
934 GACGC
HincII 817 GTCAAC GT[CT]^[GA]AC
2211 GTCAAC
HindII 817 GTCAAC GT[CT]^[GA]AC
2211 GTCAAC
MluNI 2 TGGCCA TGG^CCA
2510 TGGCCA
MscI 2 TGGCCA TGG^CCA
2510 TGGCCA
NdeI 1301 CATATG CA^TATG
2033 CATATG
NspI 734 ACATGC [GA]CATG^[CT]
1392 ACATGT
SmlI 282 CTCGAG C^T[CT][GA]AG
717 CTCAAG
SspI 1105 AATATT AAT^ATT
1812 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
Aor51HI ApoI AspHI AvaII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BsgI BsiHKAI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII Eco47III EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII