(Circular) (Six-base) MAPSORT of: pFN21AB4082.seq Check: 3602 from: 1 to: 7387 pFN21AB4082 7387 bp With 182 enzymes: SgfI * September 11, 2009 17:03 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2601 5197 278 Size: 53 83 186 936 1065 2596 2468 Fragments arranged by size: 2596 2468 1065 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2918 3372 4730 1937 Size: 981 454 1358 4594 Fragments arranged by size: 4594 1358 981 454 Acc65I G'GTAC_C Cuts at: 4708 4708 Size: 7387 AclI AA'CG_TT Cuts at: 1563 5514 5887 1563 Size: 3951 373 3063 Fragments arranged by size: 3951 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2913 4029 5450 6834 7102 1632 Size: 1281 1116 1421 1384 268 1917 Fragments arranged by size: 1917 1421 1384 1281 1116 268 AfeI AGC'GCT Cuts at: 2152 2861 4456 2152 Size: 709 1595 5083 Fragments arranged by size: 5083 1595 709 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7190 AflIII A'CryG_T Cuts at: 1203 3397 4659 6286 7263 1203 Size: 2194 1262 1627 977 1327 Fragments arranged by size: 2194 1627 1327 1262 977 AgeI A'CCGG_T Cuts at: 6218 6218 Size: 7387 AhdI GACnn_n'nnGTC Cuts at: 2326 6116 2326 Size: 3790 3597 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7387 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7387 AlwNI CAG_nnn'CTG Cuts at: 5130 6702 5130 Size: 1572 5815 ApaI G_GGCC'C Cuts at: 2362 2362 Size: 7387 ApaLI G'TGCA_C Cuts at: 5444 6600 5444 Size: 1156 6231 ApoI r'AATT_y Cuts at: 1493 2606 2885 3809 4070 4538 4696 4922 5074 Size: 1113 279 924 261 468 158 226 152 Cuts at: 5074 7183 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1113 924 468 279 261 226 158 152 AseI AT'TA_AT Cuts at: 160 1032 5941 160 Size: 872 4909 1606 Fragments arranged by size: 4909 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7387 AvaI C'yCGr_G Cuts at: 1870 1943 2287 4173 4712 1870 Size: 73 344 1886 539 4545 Fragments arranged by size: 4545 1886 539 344 73 AvrII C'CTAG_G Cuts at: 2710 2710 Size: 7387 BamHI G'GATC_C Cuts at: 3454 3649 4717 5084 3454 Size: 195 1068 367 5757 Fragments arranged by size: 5757 1068 367 195 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3845 4708 6163 618 Size: 325 217 627 2058 863 1455 1842 Fragments arranged by size: 2058 1842 1455 863 627 325 217 BanII G_rGCy'C Cuts at: 1391 2362 3076 4706 1391 Size: 971 714 1630 4072 Fragments arranged by size: 4072 1630 971 714 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2041 2450 3762 4017 928 Size: 971 142 409 1312 255 4298 Fragments arranged by size: 4298 1312 971 409 255 142 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5578 5612 1903 Size: 34 3641 34 3678 Fragments arranged by size: 3678 3641 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5279 6489 5279 Size: 1210 6177 BclI T'GATC_A Cuts at: 1548 3930 1548 Size: 2382 5005 BfrBI ATG'CAT Cuts at: 2788 2788 Size: 7387 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5998 136 Size: 107 122 71 631 605 4326 1525 Fragments arranged by size: 4326 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7382 7382 Size: 7387 BlpI GC'TnA_GC Cuts at: 3025 4318 4804 3025 Size: 1293 486 5608 Fragments arranged by size: 5608 1293 486 Bme1580I G_kGCm'C Cuts at: 1790 2362 3045 5448 6604 1790 Size: 572 683 2403 1156 2573 Fragments arranged by size: 2573 2403 1156 683 572 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7387 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2275 5118 6076 Size: 416 53 83 399 24 858 2843 958 Cuts at: 6076 442 Size: 1753 Fragments arranged by size: 2843 1753 958 858 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7387 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3036 3067 4232 6047 1239 Size: 1797 31 1165 1815 2579 Fragments arranged by size: 2579 1815 1797 1165 31 Bpu10I CC'TnA_GC Cuts at: 4466 4466 Size: 7387 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2707 5512 6377 6675 2707 Size: 2805 865 298 3419 Fragments arranged by size: 3419 2805 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6050 882 Size: 617 4551 2219 Fragments arranged by size: 4551 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2227 5178 493 Size: 711 1023 2951 2702 Fragments arranged by size: 2951 2702 1023 711 BsaBI GATnn'nnATC Cuts at: 7381 7381 Size: 7387 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2598 5194 5576 6211 Size: 53 83 186 936 1065 2596 382 635 Cuts at: 6211 275 Size: 1451 Fragments arranged by size: 2596 1451 1065 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4055 5820 6218 6492 6639 7050 4055 Size: 1765 398 274 147 411 4392 Fragments arranged by size: 4392 1765 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2995 3025 3690 3720 1178 Size: 30 165 30 1592 30 665 30 4845 Fragments arranged by size: 4845 1592 665 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2992 1186 Size: 1806 5581 BseYI C'CCAG_C Cuts at: 2431 2862 6590 7086 2431 Size: 431 3728 496 2732 Fragments arranged by size: 3728 2732 496 431 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2159 2189 2348 2429 1163 Size: 573 423 30 159 81 6121 Fragments arranged by size: 6121 573 423 159 81 30 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4837 5598 6626 1540 Size: 275 186 2836 761 1028 2301 Fragments arranged by size: 2836 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2040 2148 3076 5448 5533 6604 2040 Size: 108 928 2372 85 1071 2823 Fragments arranged by size: 2823 2372 1071 928 108 85 BsmI GAATG_Cn' Cuts at: 2908 4898 4991 2908 Size: 1990 93 5304 Fragments arranged by size: 5304 1990 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2040 2148 2362 3045 3076 4706 5448 Size: 399 250 108 214 683 31 1630 742 Cuts at: 5448 5533 6604 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1630 1071 742 683 399 250 214 108 85 31 BspEI T'CCGG_A Cuts at: 7050 7050 Size: 7387 BspHI T'CATG_A Cuts at: 2124 3579 3600 5276 7006 2124 Size: 1455 21 1676 1730 2505 Fragments arranged by size: 2505 1730 1676 1455 21 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3717 4742 844 Size: 2873 1025 3489 Fragments arranged by size: 3489 2873 1025 BsrBI CCG'CTC Cuts at: 1394 1823 5274 1394 Size: 429 3451 3507 Fragments arranged by size: 3507 3451 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3253 4477 5882 6056 65 Size: 1183 2005 1224 1405 174 1396 Fragments arranged by size: 2005 1405 1396 1224 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6031 6218 1700 Size: 110 4221 187 2869 Fragments arranged by size: 4221 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3315 96 Size: 3219 4168 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7387 BssSI C'ACGA_G Cuts at: 3774 5447 6459 3774 Size: 1673 1012 4702 Fragments arranged by size: 4702 1673 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2035 3035 794 Size: 1241 1000 5146 Fragments arranged by size: 5146 1241 1000 BstBI TT'CG_AA Cuts at: 6191 6191 Size: 7387 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7387 BstXI CCAn_nnnn'nTGG Cuts at: 4525 4525 Size: 7387 BstYI r'GATC_y Cuts at: 1972 3454 3649 4082 4717 5084 5469 5486 6938 Size: 1482 195 433 635 367 385 17 1452 Cuts at: 6938 7024 7036 7382 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1482 1452 635 433 385 367 346 195 86 17 12 BstZ17I GTA'TAC Cuts at: 2919 2919 Size: 7387 Bsu36I CC'TnA_GG Cuts at: 2975 2975 Size: 7387 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5896 Fragments arranged by size: 5896 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6648 Fragments arranged by size: 6648 683 56 BtsI GCAGTG_nn' Cuts at: 2553 4903 5697 5717 2553 Size: 2350 794 20 4223 Fragments arranged by size: 4223 2350 794 20 ClaI AT'CG_AT Cuts at: 1554 3153 1554 Size: 1599 5788 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2562 2597 529 Size: 35 1998 35 5319 Fragments arranged by size: 5319 1998 35 35 DraI TTT'AAA Cuts at: 4691 4691 Size: 7387 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2669 6394 809 Size: 507 1353 3725 1802 Fragments arranged by size: 3725 1802 1353 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 4515 4834 Size: 54 1425 50 275 18 177 2508 319 Cuts at: 4834 5723 7352 8 Size: 889 1629 43 Fragments arranged by size: 2508 1629 1425 889 319 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4834 1537 Size: 275 3022 4090 Fragments arranged by size: 4090 3022 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5317 1353 Size: 3964 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5974 6348 6494 387 Size: 1064 138 4385 374 146 1280 Fragments arranged by size: 4385 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3074 3074 Size: 7387 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2913 3036 3067 4029 4232 5450 6047 Size: 393 1281 123 31 962 203 1218 597 Cuts at: 6047 6834 7102 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1281 1218 962 787 597 393 268 203 123 31 EcoO109I rG'GnC_Cy Cuts at: 2358 2359 4044 2358 Size: 1 1685 5701 Fragments arranged by size: 5701 1685 1 EcoRI G'AATT_C Cuts at: 2606 2885 4538 4696 5074 2606 Size: 279 1653 158 378 4919 Fragments arranged by size: 4919 1653 378 279 158 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2671 4219 2671 Size: 1548 5839 FspI TGC'GCA Cuts at: 5893 5893 Size: 7387 HaeII r_GCGC'y Cuts at: 1678 2154 2448 2863 4458 6534 1678 Size: 476 294 415 1595 2076 2531 Fragments arranged by size: 2531 2076 1595 476 415 294 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2312 2344 2505 2537 3242 Size: 32 135 32 740 32 161 32 705 Cuts at: 3242 3274 3976 4008 4441 4473 6028 6060 6102 Size: 32 702 32 433 32 1555 32 42 Cuts at: 6102 6134 1373 Size: 32 2626 Fragments arranged by size: 2626 1555 740 705 702 433 161 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2824 4218 4731 4977 669 Size: 988 281 886 1394 513 246 3079 Fragments arranged by size: 3079 1394 988 886 513 281 246 HindIII A'AGCT_T Cuts at: 748 2961 748 Size: 2213 5174 HpaI GTT'AAC Cuts at: 4977 4977 Size: 7387 KpnI G_GTAC'C Cuts at: 4712 4712 Size: 7387 MfeI C'AATT_G Cuts at: 4986 4986 Size: 7387 MluI A'CGCG_T Cuts at: 7263 7263 Size: 7387 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3883 4480 6501 6685 1305 Size: 2578 597 2021 184 2007 Fragments arranged by size: 2578 2021 2007 597 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 4517 10 Size: 54 1425 343 177 2508 2880 Fragments arranged by size: 2880 2508 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2423 3155 3281 4523 5345 5704 5863 Size: 648 1257 732 126 1242 822 359 159 Cuts at: 5863 518 Size: 2042 Fragments arranged by size: 2042 1257 1242 822 732 648 359 159 126 MspA1I CmG'CkG Cuts at: 1606 4130 4803 5480 6628 6873 1606 Size: 2524 673 677 1148 245 2120 Fragments arranged by size: 2524 2120 1148 677 673 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5896 Fragments arranged by size: 5896 939 552 NdeI CA'TA_TG Cuts at: 387 3307 4039 387 Size: 2920 732 3735 Fragments arranged by size: 3735 2920 732 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7387 NotI GC'GGCC_GC Cuts at: 4834 4834 Size: 7387 NsiI A_TGCA'T Cuts at: 2790 2790 Size: 7387 NspI r_CATG'y Cuts at: 1132 1229 2743 3401 4745 6290 1132 Size: 97 1514 658 1344 1545 2229 Fragments arranged by size: 2229 1545 1514 1344 658 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7387 PciI A'CATG_T Cuts at: 3397 6286 3397 Size: 2889 4498 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7387 PfoI T'CCnGG_A Cuts at: 2999 4433 2999 Size: 1434 5953 PmeI GTTT'AAAC Cuts at: 4691 4691 Size: 7387 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5456 5488 6969 7001 5456 Size: 32 1481 32 5842 Fragments arranged by size: 5842 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 4044 4044 Size: 7387 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7387 PsiI TTA'TAA Cuts at: 4957 4957 Size: 7387 PspOMI G'GGCC_C Cuts at: 2358 2358 Size: 7387 PstI C_TGCA'G Cuts at: 830 4739 830 Size: 3909 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7387 SacI G_AGCT'C Cuts at: 3076 3076 Size: 7387 SalI G'TCGA_C Cuts at: 1936 4729 1936 Size: 2793 4594 SanDI GG'GwC_CC Cuts at: 4044 4044 Size: 7387 SbfI CC_TGCA'GG Cuts at: 4739 4739 Size: 7387 ScaI AGT'ACT Cuts at: 5635 7347 5635 Size: 1712 5675 SfcI C'TryA_G Cuts at: 826 1045 4735 5870 6551 6742 826 Size: 219 3690 1135 681 191 1471 Fragments arranged by size: 3690 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7387 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7387 SmaI CCC'GGG Cuts at: 1872 4714 1872 Size: 2842 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2287 2722 5491 6392 6654 820 Size: 197 926 344 435 2769 901 262 1553 Fragments arranged by size: 2769 1553 926 901 435 344 262 197 SnaBI TAC'GTA Cuts at: 493 5178 493 Size: 4685 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7387 SphI G_CATG'C Cuts at: 1132 4745 1132 Size: 3613 3774 SspI AAT'ATT Cuts at: 5 52 3112 3819 5311 5 Size: 47 3060 707 1492 2081 Fragments arranged by size: 3060 2081 1492 707 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7387 StyI C'CwwG_G Cuts at: 513 1065 2004 2710 3840 4188 4425 4826 513 Size: 552 939 706 1130 348 237 401 3074 Fragments arranged by size: 3074 1130 939 706 552 401 348 237 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2854 3833 4607 5414 5431 5584 5769 Size: 1255 1274 979 774 807 17 153 185 Cuts at: 5769 6214 325 Size: 445 1498 Fragments arranged by size: 1498 1274 1255 979 807 774 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3091 3315 4230 Size: 274 80 33 51 1443 1114 224 915 Cuts at: 4230 5633 7345 96 Size: 1403 1712 138 Fragments arranged by size: 1712 1443 1403 1114 915 274 224 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2225 2257 2995 3027 3430 3462 7347 7379 2225 Size: 32 738 32 403 32 3885 32 2233 Fragments arranged by size: 3885 2233 738 403 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7387 XbaI T'CTAG_A Cuts at: 4723 4723 Size: 7387 XhoI C'TCGA_G Cuts at: 1943 2287 1943 Size: 344 7043 XmaI C'CCGG_G Cuts at: 1870 4712 1870 Size: 2842 4545 XmnI GAAnn'nnTTC Cuts at: 2207 5516 2207 Size: 3309 4078 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2599 5195 276 Size: 53 83 186 936 1065 2596 2468 Fragments arranged by size: 2596 2468 1065 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BaeI BbeI BbvCI BplI BsiWI BsmBI DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI