Restriction map for
pFN21AB4058
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# NO CUT ENZYMES ( 128 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflIII AgeI AhdI
AlwNI Aor51HI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BanI BbeI BcgI
BciVI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV FseI FspI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tsp509I Tth111I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 96 CGCG CG^CG
AflII 1513 CTTAAG C^TTAAG
Alw44I 1207 GTGCAC G^TGCAC
AocI 1332 CCTGAGG CC^T[ACGT]AGG
ApaI 350 GGGCCC GGGCC^C
ApaLI 1207 GTGCAC G^TGCAC
AspHI 1207 GTGCAC G[AT]GC[AT]^C
AvaI 886 CCCGAG C^[CT]CG[GA]G
BalI 797 TGGCCA TGG^CCA
BbrPI 1204 CACGTG CAC^GTG
BbvCI 31 CCTCAGC CCTCAGC(-5/-2)
BclI 1098 TGATCA T^GATCA
BfrI 1513 CTTAAG C^TTAAG
BsaAI 1204 CACGTG [CT]AC^GT[GA]
BsaI 1426 GAGACC GGTCTC(1/5)
BsaWI 1499 TCCGGT [AT]^CCGG[AT]
BsiHKAI 1207 GTGCAC G[AT]GC[AT]^C
BsmAI 1426 GAGAC GTCTC(1/5)
BsoBI 886 CCCGAG C^[CT]CG[GA]G
BsrBI 11 CCGCTC CCGCTC(-3/-3)
BsrDI 1140 CATTGC GCAATG(2/0)
BsrFI 60 GCCGGT [GA]^CCGG[CT]
BstAPI 919 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 96 CGCG CG^CG
Bsu36I 1332 CCTGAGG CC^T[ACGT]AGG
CfoI 753 GCGC GCG^C
Cfr10I 60 GCCGGT [GA]^CCGG[CT]
DsaI 416 CCATGG C^C[GA][CT]GG
EaeI 797 TGGCCA [CT]^GGCC[GA]
Eco81I 1332 CCTGAGG CC^T[ACGT]AGG
EcoNI 270 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1098 TGATCA T^GATCA
HaeII 752 AGCGCC [GA]GCGC^[CT]
HhaI 753 GCGC GCG^C
HinP1I 753 GCGC G^CGC
HphI 1552 GGTGA GGTGA(8/7)
MluNI 797 TGGCCA TGG^CCA
MscI 797 TGGCCA TGG^CCA
MvnI 96 CGCG CG^CG
NcoI 416 CCATGG C^CATGG
NspI 39 GCATGC [GA]CATG^[CT]
PmaCI 1204 CACGTG CAC^GTG
PmlI 1204 CACGTG CAC^GTG
ScaI 773 AGTACT AGT^ACT
SphI 39 GCATGC GCATG^C
TfiI 455 GATTC G^A[AT]TC
XbaI 1521 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
BbsI 1199 GTCTTC GAAGAC(2/6)
1487 GTCTTC
BpuAI 1199 GTCTTC GAAGAC(2/6)
1487 GTCTTC
BsgI 565 CTGCAC GTGCAG(16/14)
738 CTGCAC
EcoT22I 82 ATGCAT ATGCA^T
1415 ATGCAT
HgaI 97 GCGTC GACGC(5/10)
834 GCGTC
MaeII 1205 ACGT A^CGT
1485 ACGT
NsiI 82 ATGCAT ATGCA^T
1415 ATGCAT
PflMI 331 CCATCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1599 CCATCAACTGG
PleI 235 GACTC GAGTC(4/5)
281 GAGTC
PvuII 660 CAGCTG CAG^CTG
1025 CAGCTG
SmlI 1513 CTTAAG C^T[CT][GA]AG
1588 CTTGAG
TaqI 1173 TCGA T^CGA
1565 TCGA
TthHB8I 1173 TCGA T^CGA
1565 TCGA
Van91I 331 CCATCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1599 CCATCAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BamHI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI StyI Tru9I
TseI Tsp45I TspRI XhoII