Restriction map for
pFN21AB4058
(Linear) (Six-base) MAPSORT of: KIBB4058.seq Check: 3500 from: 1 to: 1611
KIBB4058.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 1611
Size: 548 1063
AflII C'TTAA_G
Cuts at: 0 1513 1611
Size: 1513 98
ApaI G_GGCC'C
Cuts at: 0 354 1611
Size: 354 1257
ApaLI G'TGCA_C
Cuts at: 0 1207 1611
Size: 1207 404
AvaI C'yCGr_G
Cuts at: 0 886 1611
Size: 886 725
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1429 1462 1611
Size: 1429 33 149
Fragments arranged by size:
1429 149 33
BamHI G'GATC_C
Cuts at: 0 129 908 1067 1611
Size: 129 779 159 544
Fragments arranged by size:
779 544 159 129
BanII G_rGCy'C
Cuts at: 0 354 391 885 1611
Size: 354 37 494 726
Fragments arranged by size:
726 494 354 37
BbsI GAAGACnn'nnnn_
Cuts at: 0 1192 1480 1611
Size: 1192 288 131
Fragments arranged by size:
1192 288 131
BbvCI CC'TCA_GC
Cuts at: 0 32 1611
Size: 32 1579
BclI T'GATC_A
Cuts at: 0 1098 1611
Size: 1098 513
BfrBI ATG'CAT
Cuts at: 0 84 1417 1611
Size: 84 1333 194
Fragments arranged by size:
1333 194 84
Bme1580I G_kGCm'C
Cuts at: 0 354 1211 1611
Size: 354 857 400
Fragments arranged by size:
857 400 354
BmrI ACTGGGnnnn_n'
Cuts at: 0 394 1611
Size: 394 1217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 566 934 1248 1444 1611
Size: 425 141 368 314 196 167
Fragments arranged by size:
425 368 314 196 167 141
Bpu10I CC'TnA_GC
Cuts at: 0 32 636 1451 1611
Size: 32 604 815 160
Fragments arranged by size:
815 604 160 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1609 1611
Size: 1609 2
BsaI GGTCTCn'nnnn_
Cuts at: 0 1420 1611
Size: 1420 191
BsaAI yAC'GTr
Cuts at: 0 1206 1611
Size: 1206 405
BsaWI w'CCGG_w
Cuts at: 0 1499 1611
Size: 1499 112
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 241 271 1066 1096 1396 1426 1611
Size: 241 30 795 30 300 30 185
Fragments arranged by size:
795 300 241 185 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 246 287 844 904 1126 1611
Size: 246 41 557 60 222 485
Fragments arranged by size:
557 485 246 222 60 41
BseYI C'CCAG_C
Cuts at: 0 874 1611
Size: 874 737
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 723 1611
Size: 550 173 888
Fragments arranged by size:
888 550 173
BsiHKAI G_wGCw'C
Cuts at: 0 1211 1611
Size: 1211 400
Bsp1286I G_dGCh'C
Cuts at: 0 354 391 885 1211 1611
Size: 354 37 494 326 400
Fragments arranged by size:
494 400 354 326 37
BsrBI CCG'CTC
Cuts at: 0 13 1611
Size: 13 1598
BsrDI GCAATG_nn'
Cuts at: 0 1139 1611
Size: 1139 472
BsrFI r'CCGG_y
Cuts at: 0 60 1611
Size: 60 1551
BstAPI GCAn_nnn'nTGC
Cuts at: 0 925 1611
Size: 925 686
BstYI r'GATC_y
Cuts at: 0 129 908 1067 1611
Size: 129 779 159 544
Fragments arranged by size:
779 544 159 129
Bsu36I CC'TnA_GG
Cuts at: 0 1333 1611
Size: 1333 278
BtgI C'CryG_G
Cuts at: 0 416 1611
Size: 416 1195
BtsI GCAGTG_nn'
Cuts at: 0 58 1611
Size: 58 1553
EaeI y'GGCC_r
Cuts at: 0 797 1611
Size: 797 814
EarI CTCTTCn'nnn_
Cuts at: 0 805 934 1057 1611
Size: 805 129 123 554
Fragments arranged by size:
805 554 129 123
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 179 1611
Size: 179 1432
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 425 548 566 934 1248 1444 1611
Size: 425 123 18 368 314 196 167
Fragments arranged by size:
425 368 314 196 167 123 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 274 1611
Size: 274 1337
EcoO109I rG'GnC_Cy
Cuts at: 0 350 351 422 561 961 1368 1395 1611
Size: 350 1 71 139 400 407 27 216
Fragments arranged by size:
407 400 350 216 139 71 27 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 699 1611
Size: 699 912
HaeII r_GCGC'y
Cuts at: 0 756 1611
Size: 756 855
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 241 273 619 651 1166 1198 1270 1302
Size: 241 32 346 32 515 32 72 32
Cuts at: 1302 1611
Size: 309
Fragments arranged by size:
515 346 309 241 72 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1400 1611
Size: 1400 211
MscI TGG'CCA
Cuts at: 0 799 1611
Size: 799 812
MslI CAynn'nnrTG
Cuts at: 0 805 1306 1582 1611
Size: 805 501 276 29
Fragments arranged by size:
805 501 276 29
MspA1I CmG'CkG
Cuts at: 0 36 139 227 662 759 767 840 1027
Size: 36 103 88 435 97 8 73 187
Cuts at: 1027 1611
Size: 584
Fragments arranged by size:
584 435 187 103 97 88 73 36
8
NcoI C'CATG_G
Cuts at: 0 416 1611
Size: 416 1195
NsiI A_TGCA'T
Cuts at: 0 86 1419 1611
Size: 86 1333 192
Fragments arranged by size:
1333 192 86
NspI r_CATG'y
Cuts at: 0 43 1611
Size: 43 1568
PasI CC'CwG_GG
Cuts at: 0 276 505 592 1611
Size: 276 229 87 1019
Fragments arranged by size:
1019 276 229 87
PflMI CCAn_nnn'nTGG
Cuts at: 0 337 1605 1611
Size: 337 1268 6
Fragments arranged by size:
1268 337 6
PfoI T'CCnGG_A
Cuts at: 0 911 1611
Size: 911 700
PmlI CAC'GTG
Cuts at: 0 1206 1611
Size: 1206 405
PpuMI rG'GwC_Cy
Cuts at: 0 422 961 1395 1611
Size: 422 539 434 216
Fragments arranged by size:
539 434 422 216
PspOMI G'GGCC_C
Cuts at: 0 350 1611
Size: 350 1261
PvuII CAG'CTG
Cuts at: 0 662 1027 1611
Size: 662 365 584
Fragments arranged by size:
662 584 365
SanDI GG'GwC_CC
Cuts at: 0 422 1611
Size: 422 1189
ScaI AGT'ACT
Cuts at: 0 775 1611
Size: 775 836
SmlI C'TyrA_G
Cuts at: 0 1513 1588 1611
Size: 1513 75 23
Fragments arranged by size:
1513 75 23
SphI G_CATG'C
Cuts at: 0 43 1611
Size: 43 1568
StyI C'CwwG_G
Cuts at: 0 336 416 1364 1611
Size: 336 80 948 247
Fragments arranged by size:
948 336 247 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1244 1611
Size: 1244 367
TatI w'GTAC_w
Cuts at: 0 773 1611
Size: 773 838
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1066 1098 1611
Size: 1066 32 513
Fragments arranged by size:
1066 513 32
XbaI T'CTAG_A
Cuts at: 0 1521 1611
Size: 1521 90
Enzymes that do cut:
AcuI AflII ApaI ApaLI AvaI BaeI BamHI BanII
BbsI BbvCI BclI BfrBI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BsrBI BsrDI BsrFI BstAPI BstYI Bsu36I
BtgI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I MmeI MscI MslI MspA1I NcoI
NsiI NspI PasI PflMI PfoI PmlI PpuMI PspOMI
PvuII SanDI ScaI SmlI SphI StyI TaqII TatI
TstI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApoI AscI AseI
AsiSI AvrII BanI BbeI BcgI BciVI BglI BglII
BlpI BmgBI BmtI BplI BsaBI BsaHI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrGI BssHII BssSI
BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI PacI
PciI PmeI PpiI PshAI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SapI SbfI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI StuI SwaI Tth111I XcmI XhoI XmaI
XmnI ZraI