(Circular) (Six-base) MAPSORT of: pFN21AB4031.seq Check: 6019 from: 1 to: 7075 pFN21AB4031 7075 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4885 278 Size: 53 83 186 936 3349 2468 Fragments arranged by size: 3349 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3234 4418 1937 Size: 1297 1184 4594 Fragments arranged by size: 4594 1297 1184 Acc65I G'GTAC_C Cuts at: 4396 4396 Size: 7075 AclI AA'CG_TT Cuts at: 1563 5202 5575 1563 Size: 3639 373 3063 Fragments arranged by size: 3639 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2106 2854 2856 3541 3676 3864 3983 4073 Size: 474 748 2 685 135 188 119 90 Cuts at: 4073 5138 6522 6790 1632 Size: 1065 1384 268 1917 Fragments arranged by size: 1917 1384 1065 748 685 474 268 188 135 119 90 2 AflII C'TTAA_G Cuts at: 820 1017 2209 820 Size: 197 1192 5686 Fragments arranged by size: 5686 1192 197 AflIII A'CryG_T Cuts at: 1203 3757 3955 5974 6951 1203 Size: 2554 198 2019 977 1327 Fragments arranged by size: 2554 2019 1327 977 198 AgeI A'CCGG_T Cuts at: 5906 5906 Size: 7075 AhdI GACnn_n'nnGTC Cuts at: 5804 5804 Size: 7075 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7075 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4377 1184 Size: 3193 3882 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2733 2765 2733 Size: 32 7043 AlwNI CAG_nnn'CTG Cuts at: 3655 4818 6390 3655 Size: 1163 1572 4340 Fragments arranged by size: 4340 1572 1163 ApaI G_GGCC'C Cuts at: 2707 2707 Size: 7075 ApaLI G'TGCA_C Cuts at: 5132 6288 5132 Size: 1156 5919 ApoI r'AATT_y Cuts at: 1493 2312 2455 3921 4384 4610 4762 6871 1493 Size: 819 143 1466 463 226 152 2109 1697 Fragments arranged by size: 2109 1697 1466 819 463 226 152 143 AseI AT'TA_AT Cuts at: 160 1032 3246 5629 160 Size: 872 2214 2383 1606 Fragments arranged by size: 2383 2214 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7075 AvaI C'yCGr_G Cuts at: 1870 1943 2897 4400 1870 Size: 73 954 1503 4545 Fragments arranged by size: 4545 1503 954 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2619 2652 2619 Size: 33 7042 BamHI G'GATC_C Cuts at: 4405 4772 4405 Size: 367 6708 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4396 5851 618 Size: 325 217 627 2609 1455 1842 Fragments arranged by size: 2609 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2707 4394 1391 Size: 1316 1687 4072 Fragments arranged by size: 4072 1687 1316 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2999 3712 928 Size: 971 1100 713 4291 Fragments arranged by size: 4291 1100 971 713 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5266 5300 1903 Size: 34 3329 34 3678 Fragments arranged by size: 3678 3329 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4967 6177 4967 Size: 1210 5865 BclI T'GATC_A Cuts at: 1548 1548 Size: 7075 BfrBI ATG'CAT Cuts at: 2519 2519 Size: 7075 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4370 5686 136 Size: 107 122 71 631 605 2698 1316 1525 Fragments arranged by size: 2698 1525 1316 631 605 122 107 71 BglII A'GATC_T Cuts at: 2205 4251 7070 2205 Size: 2046 2819 2210 Fragments arranged by size: 2819 2210 2046 BlpI GC'TnA_GC Cuts at: 4492 4492 Size: 7075 Bme1580I G_kGCm'C Cuts at: 1790 2707 4366 5136 6292 1790 Size: 917 1659 770 1156 2573 Fragments arranged by size: 2573 1659 1156 917 770 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7075 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4806 5764 442 Size: 416 53 83 399 24 3389 958 1753 Fragments arranged by size: 3389 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7075 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3805 4319 5735 1239 Size: 2566 514 1416 2579 Fragments arranged by size: 2579 2566 1416 514 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4088 5200 6065 6363 4088 Size: 1112 865 298 4800 Fragments arranged by size: 4800 1112 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5738 882 Size: 617 4239 2219 Fragments arranged by size: 4239 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4866 493 Size: 711 3662 2702 Fragments arranged by size: 3662 2702 711 BsaBI GATnn'nnATC Cuts at: 7069 7069 Size: 7075 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2620 4882 5264 5899 Size: 53 83 186 936 1087 2262 382 635 Cuts at: 5899 275 Size: 1451 Fragments arranged by size: 2262 1451 1087 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5508 5906 6180 6327 6738 5508 Size: 398 274 147 411 5845 Fragments arranged by size: 5845 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2301 2331 2809 2839 1178 Size: 30 165 30 898 30 478 30 5414 Fragments arranged by size: 5414 898 478 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2817 3119 1186 Size: 1631 302 5142 Fragments arranged by size: 5142 1631 302 BseYI C'CCAG_C Cuts at: 3291 6278 6774 3291 Size: 2987 496 3592 Fragments arranged by size: 3592 2987 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2988 1163 Size: 573 1252 5250 Fragments arranged by size: 5250 1252 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4525 5286 6314 1540 Size: 275 186 2524 761 1028 2301 Fragments arranged by size: 2524 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3700 4176 5136 5221 6292 3700 Size: 476 960 85 1071 4483 Fragments arranged by size: 4483 1071 960 476 85 BsmI GAATG_Cn' Cuts at: 2290 3561 4586 4679 2290 Size: 1271 1025 93 4686 Fragments arranged by size: 4686 1271 1025 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2707 3700 4176 4366 4394 5136 5221 Size: 399 917 993 476 190 28 742 85 Cuts at: 5221 6292 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 993 917 742 476 399 190 85 28 BspEI T'CCGG_A Cuts at: 6738 6738 Size: 7075 BspHI T'CATG_A Cuts at: 3712 4158 4964 6694 3712 Size: 446 806 1730 4093 Fragments arranged by size: 4093 1730 806 446 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3458 4430 844 Size: 2614 972 3489 Fragments arranged by size: 3489 2614 972 BsrBI CCG'CTC Cuts at: 1394 1823 4962 1394 Size: 429 3139 3507 Fragments arranged by size: 3507 3139 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5570 5744 65 Size: 1183 4322 174 1396 Fragments arranged by size: 4322 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5719 5906 1700 Size: 110 3909 187 2869 Fragments arranged by size: 3909 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2222 3117 3646 96 Size: 2126 895 529 3525 Fragments arranged by size: 3525 2126 895 529 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7075 BssSI C'ACGA_G Cuts at: 3891 5135 6147 3891 Size: 1244 1012 4819 Fragments arranged by size: 4819 1244 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2806 794 Size: 2012 5063 BstBI TT'CG_AA Cuts at: 5879 5879 Size: 7075 BstEII G'GTnAC_C Cuts at: 1184 4291 1184 Size: 3107 3968 BstYI r'GATC_y Cuts at: 1972 2205 3036 3322 3870 3966 4251 4405 4772 Size: 233 831 286 548 96 285 154 367 Cuts at: 4772 5157 5174 6626 6712 6724 7070 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 831 548 385 367 346 286 285 233 154 96 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2490 2490 Size: 7075 BtgI C'CryG_G Cuts at: 513 1065 4217 513 Size: 552 3152 3371 Fragments arranged by size: 3371 3152 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6336 Fragments arranged by size: 6336 683 56 BtsI GCAGTG_nn' Cuts at: 2792 2959 4051 4314 4591 5385 5405 2792 Size: 167 1092 263 277 794 20 4462 Fragments arranged by size: 4462 1092 794 277 263 167 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7075 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2287 2322 529 Size: 35 1723 35 5282 Fragments arranged by size: 5282 1723 35 35 DraI TTT'AAA Cuts at: 3529 4379 3529 Size: 850 6225 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6082 809 Size: 507 4766 1802 Fragments arranged by size: 4766 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4341 4522 5411 Size: 54 1425 50 275 18 2511 181 889 Cuts at: 5411 7040 8 Size: 1629 43 Fragments arranged by size: 2511 1629 1425 889 275 181 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4522 1537 Size: 275 2710 4090 Fragments arranged by size: 4090 2710 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2676 2943 3492 3883 4316 5005 1353 Size: 1323 267 549 391 433 689 3423 Fragments arranged by size: 3423 1323 689 549 433 391 267 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5662 6036 6182 387 Size: 1064 138 4073 374 146 1280 Fragments arranged by size: 4073 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2106 2854 2856 3541 3676 3805 3864 Size: 393 474 748 2 685 135 129 59 Cuts at: 3864 3983 4073 4319 5138 5735 6522 6790 1239 Size: 119 90 246 819 597 787 268 1524 Fragments arranged by size: 1524 819 787 748 685 597 474 393 268 246 135 129 119 90 59 2 EcoO109I rG'GnC_Cy Cuts at: 2903 2903 Size: 7075 EcoRI G'AATT_C Cuts at: 4384 4762 4384 Size: 378 6697 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2201 2972 3012 3058 4016 2201 Size: 771 40 46 958 5260 Fragments arranged by size: 5260 958 771 46 40 FspI TGC'GCA Cuts at: 5581 5581 Size: 7075 HaeII r_GCGC'y Cuts at: 1678 6222 1678 Size: 4544 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3778 3810 5716 5748 5790 Size: 32 135 32 2206 32 1906 32 42 Cuts at: 5790 5822 1373 Size: 32 2626 Fragments arranged by size: 2626 2206 1906 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2336 4419 4665 669 Size: 988 281 398 2083 246 3079 Fragments arranged by size: 3079 2083 988 398 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7075 HpaI GTT'AAC Cuts at: 4665 4665 Size: 7075 KpnI G_GTAC'C Cuts at: 4400 4400 Size: 7075 MfeI C'AATT_G Cuts at: 4674 4674 Size: 7075 MluI A'CGCG_T Cuts at: 6951 6951 Size: 7075 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2254 2524 3266 6189 6373 1305 Size: 949 270 742 2923 184 2007 Fragments arranged by size: 2923 2007 949 742 270 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5253 Fragments arranged by size: 5253 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3108 3110 5033 5392 5551 518 Size: 648 1942 2 1923 359 159 2042 Fragments arranged by size: 2042 1942 1923 648 359 159 2 MspA1I CmG'CkG Cuts at: 1606 4150 4491 5168 6316 6561 1606 Size: 2544 341 677 1148 245 2120 Fragments arranged by size: 2544 2120 1148 677 341 245 NcoI C'CATG_G Cuts at: 513 1065 4217 513 Size: 552 3152 3371 Fragments arranged by size: 3371 3152 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7075 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7075 NotI GC'GGCC_GC Cuts at: 4522 4522 Size: 7075 NsiI A_TGCA'T Cuts at: 2521 2521 Size: 7075 NspI r_CATG'y Cuts at: 1132 1229 3205 3761 3959 4433 5978 1132 Size: 97 1976 556 198 474 1545 2229 Fragments arranged by size: 2229 1976 1545 556 474 198 97 PasI CC'CwG_GG Cuts at: 1348 2538 1348 Size: 1190 5885 PciI A'CATG_T Cuts at: 3757 3955 5974 3757 Size: 198 2019 4858 Fragments arranged by size: 4858 2019 198 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7075 PfoI T'CCnGG_A Cuts at: 3490 3490 Size: 7075 PmeI GTTT'AAAC Cuts at: 4379 4379 Size: 7075 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5144 5176 6657 6689 5144 Size: 32 1481 32 5530 Fragments arranged by size: 5530 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2903 2903 Size: 7075 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7075 PsiI TTA'TAA Cuts at: 2367 4645 2367 Size: 2278 4797 PspOMI G'GGCC_C Cuts at: 2703 2703 Size: 7075 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3342 3374 3342 Size: 32 7043 PstI C_TGCA'G Cuts at: 830 2799 4248 4371 4427 830 Size: 1969 1449 123 56 3478 Fragments arranged by size: 3478 1969 1449 123 56 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7075 SalI G'TCGA_C Cuts at: 1936 4417 1936 Size: 2481 4594 SbfI CC_TGCA'GG Cuts at: 4371 4427 4371 Size: 56 7019 ScaI AGT'ACT Cuts at: 5323 7035 5323 Size: 1712 5363 SfcI C'TryA_G Cuts at: 826 1045 2795 4244 4259 4367 4423 5558 6239 Size: 219 1750 1449 15 108 56 1135 681 Cuts at: 6239 6430 826 Size: 191 1471 Fragments arranged by size: 1750 1471 1449 1135 681 219 191 108 56 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7075 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7075 SmaI CCC'GGG Cuts at: 1872 4402 1872 Size: 2530 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2209 4067 5179 6080 6342 820 Size: 197 926 266 1858 1112 901 262 1553 Fragments arranged by size: 1858 1553 1112 926 901 266 262 197 SnaBI TAC'GTA Cuts at: 493 4866 493 Size: 4373 2702 SpeI A'CTAG_T Cuts at: 152 3814 152 Size: 3662 3413 SphI G_CATG'C Cuts at: 1132 4433 1132 Size: 3301 3774 SspI AAT'ATT Cuts at: 5 52 4999 5 Size: 47 4947 2081 Fragments arranged by size: 4947 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7075 StyI C'CwwG_G Cuts at: 513 1065 2858 4217 4514 513 Size: 552 1793 1359 297 3074 Fragments arranged by size: 3074 1793 1359 552 297 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4184 5102 5119 5272 5457 5902 325 Size: 1255 2604 918 17 153 185 445 1498 Fragments arranged by size: 2604 1498 1255 918 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2222 3117 3646 Size: 274 80 33 51 1443 245 895 529 Cuts at: 3646 3811 4284 5321 7033 96 Size: 165 473 1037 1712 138 Fragments arranged by size: 1712 1443 1037 895 529 473 274 245 165 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2878 2910 2983 3015 7035 7067 2878 Size: 32 73 32 4020 32 2886 Fragments arranged by size: 4020 2886 73 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7075 XbaI T'CTAG_A Cuts at: 3361 4411 3361 Size: 1050 6025 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7075 XmaI C'CCGG_G Cuts at: 1870 4400 1870 Size: 2530 4545 XmnI GAAnn'nnTTC Cuts at: 4086 4130 5204 4086 Size: 44 1074 5957 Fragments arranged by size: 5957 1074 44 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4883 276 Size: 53 83 186 936 3349 2468 Fragments arranged by size: 3349 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI