Restriction map for
pFN21AB4031
(Linear) (Six-base) MAPSORT of: KIBB4031.seq Check: 8654 from: 1 to: 2370
KIBB4031.orf
With 182 enzymes: SgfI *
February 28, 2007 11:25 ..
AccI GT'mk_AC
Cuts at: 0 1229 2370
Size: 1229 1141
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 849 851 1536 1671 1859 1978 2068
Size: 101 748 2 685 135 188 119 90
Cuts at: 2068 2370
Size: 302
Fragments arranged by size:
748 685 302 188 135 119 101 90
2
AflII C'TTAA_G
Cuts at: 0 204 2370
Size: 204 2166
AflIII A'CryG_T
Cuts at: 0 1752 1950 2370
Size: 1752 198 420
Fragments arranged by size:
1752 420 198
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 728 760 2370
Size: 728 32 1610
Fragments arranged by size:
1610 728 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1650 2370
Size: 1650 720
ApaI G_GGCC'C
Cuts at: 0 702 2370
Size: 702 1668
ApoI r'AATT_y
Cuts at: 0 307 450 1916 2370
Size: 307 143 1466 454
Fragments arranged by size:
1466 454 307 143
AseI AT'TA_AT
Cuts at: 0 1241 2370
Size: 1241 1129
AvaI C'yCGr_G
Cuts at: 0 892 2370
Size: 892 1478
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 614 647 2370
Size: 614 33 1723
Fragments arranged by size:
1723 614 33
BanII G_rGCy'C
Cuts at: 0 702 2370
Size: 702 1668
BbsI GAAGACnn'nnnn_
Cuts at: 0 994 1707 2370
Size: 994 713 663
Fragments arranged by size:
994 713 663
BfrBI ATG'CAT
Cuts at: 0 514 2370
Size: 514 1856
BglI GCCn_nnn'nGGC
Cuts at: 0 2365 2370
Size: 2365 5
BglII A'GATC_T
Cuts at: 0 200 2246 2370
Size: 200 2046 124
Fragments arranged by size:
2046 200 124
Bme1580I G_kGCm'C
Cuts at: 0 702 2361 2370
Size: 702 1659 9
Fragments arranged by size:
1659 702 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1800 2314 2370
Size: 1800 514 56
Fragments arranged by size:
1800 514 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2083 2370
Size: 2083 287
BsaHI Gr'CG_yC
Cuts at: 0 615 2370
Size: 615 1755
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 296 326 804 834 2370
Size: 296 30 478 30 1536
Fragments arranged by size:
1536 478 296 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 812 1114 2370
Size: 812 302 1256
Fragments arranged by size:
1256 812 302
BseYI C'CCAG_C
Cuts at: 0 1286 2370
Size: 1286 1084
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 983 2370
Size: 983 1387
BsiHKAI G_wGCw'C
Cuts at: 0 1695 2171 2370
Size: 1695 476 199
Fragments arranged by size:
1695 476 199
BsmI GAATG_Cn'
Cuts at: 0 285 1556 2370
Size: 285 1271 814
Fragments arranged by size:
1271 814 285
Bsp1286I G_dGCh'C
Cuts at: 0 702 1695 2171 2361 2370
Size: 702 993 476 190 9
Fragments arranged by size:
993 702 476 190 9
BspHI T'CATG_A
Cuts at: 0 1707 2153 2370
Size: 1707 446 217
Fragments arranged by size:
1707 446 217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1453 2370
Size: 1453 917
BsrGI T'GTAC_A
Cuts at: 0 217 1112 1641 2370
Size: 217 895 529 729
Fragments arranged by size:
895 729 529 217
BssSI C'ACGA_G
Cuts at: 0 1886 2370
Size: 1886 484
BstAPI GCAn_nnn'nTGC
Cuts at: 0 801 2370
Size: 801 1569
BstEII G'GTnAC_C
Cuts at: 0 2286 2370
Size: 2286 84
BstYI r'GATC_y
Cuts at: 0 200 1031 1317 1865 1961 2246 2370
Size: 200 831 286 548 96 285 124
Fragments arranged by size:
831 548 286 285 200 124 96
Bsu36I CC'TnA_GG
Cuts at: 0 485 2370
Size: 485 1885
BtgI C'CryG_G
Cuts at: 0 2212 2370
Size: 2212 158
BtsI GCAGTG_nn'
Cuts at: 0 787 954 2046 2309 2370
Size: 787 167 1092 263 61
Fragments arranged by size:
1092 787 263 167 61
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 282 317 2370
Size: 282 35 2053
Fragments arranged by size:
2053 282 35
DraI TTT'AAA
Cuts at: 0 1524 2370
Size: 1524 846
EaeI y'GGCC_r
Cuts at: 0 2336 2370
Size: 2336 34
EarI CTCTTCn'nnn_
Cuts at: 0 671 938 1487 1878 2311 2370
Size: 671 267 549 391 433 59
Fragments arranged by size:
671 549 433 391 267 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 849 851 1536 1671 1800 1859 1978
Size: 101 748 2 685 135 129 59 119
Cuts at: 1978 2068 2314 2370
Size: 90 246 56
Fragments arranged by size:
748 685 246 135 129 119 101 90
59 56 2
EcoO109I rG'GnC_Cy
Cuts at: 0 898 2370
Size: 898 1472
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 196 967 1007 1053 2011 2370
Size: 196 771 40 46 958 359
Fragments arranged by size:
958 771 359 196 46 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1773 1805 2370
Size: 1773 32 565
Fragments arranged by size:
1773 565 32
HincII GTy'rAC
Cuts at: 0 331 2370
Size: 331 2039
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 519 1261 2370
Size: 249 270 742 1109
Fragments arranged by size:
1109 742 270 249
MslI CAynn'nnrTG
Cuts at: 0 1103 1105 2370
Size: 1103 2 1265
Fragments arranged by size:
1265 1103 2
MspA1I CmG'CkG
Cuts at: 0 2145 2370
Size: 2145 225
NcoI C'CATG_G
Cuts at: 0 2212 2370
Size: 2212 158
NsiI A_TGCA'T
Cuts at: 0 516 2370
Size: 516 1854
NspI r_CATG'y
Cuts at: 0 1200 1756 1954 2370
Size: 1200 556 198 416
Fragments arranged by size:
1200 556 416 198
PasI CC'CwG_GG
Cuts at: 0 533 2370
Size: 533 1837
PciI A'CATG_T
Cuts at: 0 1752 1950 2370
Size: 1752 198 420
Fragments arranged by size:
1752 420 198
PfoI T'CCnGG_A
Cuts at: 0 1485 2370
Size: 1485 885
PpuMI rG'GwC_Cy
Cuts at: 0 898 2370
Size: 898 1472
PsiI TTA'TAA
Cuts at: 0 362 2370
Size: 362 2008
PspOMI G'GGCC_C
Cuts at: 0 698 2370
Size: 698 1672
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1337 1369 2370
Size: 1337 32 1001
Fragments arranged by size:
1337 1001 32
PstI C_TGCA'G
Cuts at: 0 794 2243 2366 2370
Size: 794 1449 123 4
Fragments arranged by size:
1449 794 123 4
SbfI CC_TGCA'GG
Cuts at: 0 2366 2370
Size: 2366 4
SfcI C'TryA_G
Cuts at: 0 790 2239 2254 2362 2370
Size: 790 1449 15 108 8
Fragments arranged by size:
1449 790 108 15 8
SmlI C'TyrA_G
Cuts at: 0 204 2062 2370
Size: 204 1858 308
Fragments arranged by size:
1858 308 204
SpeI A'CTAG_T
Cuts at: 0 1809 2370
Size: 1809 561
StyI C'CwwG_G
Cuts at: 0 853 2212 2370
Size: 853 1359 158
Fragments arranged by size:
1359 853 158
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2179 2370
Size: 2179 191
TatI w'GTAC_w
Cuts at: 0 217 1112 1641 1806 2279 2370
Size: 217 895 529 165 473 91
Fragments arranged by size:
895 529 473 217 165 91
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 873 905 978 1010 2370
Size: 873 32 73 32 1360
Fragments arranged by size:
1360 873 73 32 32
XbaI T'CTAG_A
Cuts at: 0 1356 2370
Size: 1356 1014
XmnI GAAnn'nnTTC
Cuts at: 0 2081 2125 2370
Size: 2081 44 245
Fragments arranged by size:
2081 245 44
Enzymes that do cut:
AccI AcuI AflII AflIII AloI AlwNI ApaI ApoI
AseI AvaI BaeI BanII BbsI BfrBI BglI BglII
Bme1580I BpmI BpuEI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrGI BssSI BstAPI
BstEII BstYI Bsu36I BtgI BtsI CspCI DraI EaeI
EarI Eco57MI EcoO109I FalI Hin4I HincII MmeI MslI
MspA1I NcoI NsiI NspI PasI PciI PfoI PpuMI
PsiI PspOMI PsrI PstI SbfI SfcI SmlI SpeI
StyI TaqII TatI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AleI
AlfI ApaLI AscI AsiSI AvrII BamHI BanI BbeI
BbvCI BcgI BciVI BclI BlpI BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaWI BsiEI BsiWI
BsmBI BspEI BsrBI BsrDI BsrFI BssHII BstBI BstXI
BstZ17I BtgZI ClaI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI PacI PflMI PmeI
PmlI PpiI PshAI PspXI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI SspI StuI
SwaI Tth111I XcmI XhoI XmaI ZraI