(Circular) (Six-base) MAPSORT of: pFN21AB3935.seq Check: 2182 from: 1 to: 5971 pFN21AB3935 5971 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3064 3064 Size: 5971 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3781 278 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3314 1937 Size: 1377 4594 Acc65I G'GTAC_C Cuts at: 2647 3292 2647 Size: 645 5326 AclI AA'CG_TT Cuts at: 1563 4098 4471 1563 Size: 2535 373 3063 Fragments arranged by size: 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2153 2426 2924 2996 4034 5418 5686 1632 Size: 521 273 498 72 1038 1384 268 1917 Fragments arranged by size: 1917 1384 1038 521 498 273 268 72 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5774 AflIII A'CryG_T Cuts at: 1203 4870 5847 1203 Size: 3667 977 1327 Fragments arranged by size: 3667 1327 977 AgeI A'CCGG_T Cuts at: 4802 4802 Size: 5971 AhdI GACnn_n'nnGTC Cuts at: 4700 4700 Size: 5971 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5971 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5971 AlwNI CAG_nnn'CTG Cuts at: 2482 3714 5286 2482 Size: 1232 1572 3167 Fragments arranged by size: 3167 1572 1232 ApaLI G'TGCA_C Cuts at: 4028 5184 4028 Size: 1156 4815 ApoI r'AATT_y Cuts at: 1493 2163 2582 2732 3280 3506 3658 5767 1493 Size: 670 419 150 548 226 152 2109 1697 Fragments arranged by size: 2109 1697 670 548 419 226 152 150 AseI AT'TA_AT Cuts at: 160 1032 4525 160 Size: 872 3493 1606 Fragments arranged by size: 3493 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 3296 1870 Size: 73 1353 4545 Fragments arranged by size: 4545 1353 73 BamHI G'GATC_C Cuts at: 3301 3668 3301 Size: 367 5604 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2647 3292 4747 618 Size: 325 217 627 860 645 1455 1842 Fragments arranged by size: 1842 1455 860 645 627 325 217 BanII G_rGCy'C Cuts at: 1391 3290 1391 Size: 1899 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2345 2614 928 Size: 971 446 269 4285 Fragments arranged by size: 4285 971 446 269 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2050 2084 4162 4196 1903 Size: 34 113 34 2078 34 3678 Fragments arranged by size: 3678 2078 113 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3863 5073 3863 Size: 1210 4761 BclI T'GATC_A Cuts at: 1548 1548 Size: 5971 BfrBI ATG'CAT Cuts at: 2120 2120 Size: 5971 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3257 4582 136 Size: 107 122 71 631 605 1585 1325 1525 Fragments arranged by size: 1585 1525 1325 631 605 122 107 71 BglII A'GATC_T Cuts at: 5966 5966 Size: 5971 BlpI GC'TnA_GC Cuts at: 3388 3388 Size: 5971 Bme1580I G_kGCm'C Cuts at: 1790 2534 4032 5188 1790 Size: 744 1498 1156 2573 Fragments arranged by size: 2573 1498 1156 744 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5971 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2441 3208 3702 Size: 416 53 83 399 24 1024 767 494 Cuts at: 3702 4660 442 Size: 958 1753 Fragments arranged by size: 1753 1024 958 767 494 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2121 2909 4631 1239 Size: 882 788 1722 2579 Fragments arranged by size: 2579 1722 882 788 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2345 4096 4961 5259 2345 Size: 1751 865 298 3057 Fragments arranged by size: 3057 1751 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4634 882 Size: 617 3135 2219 Fragments arranged by size: 3135 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2548 2854 3762 493 Size: 711 1344 306 908 2702 Fragments arranged by size: 2702 1344 908 711 306 BsaBI GATnn'nnATC Cuts at: 2700 5965 2700 Size: 3265 2706 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3778 4160 4795 275 Size: 53 83 186 936 2245 382 635 1451 Fragments arranged by size: 2245 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4404 4802 5076 5223 5634 4404 Size: 398 274 147 411 4741 Fragments arranged by size: 4741 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5746 Fragments arranged by size: 5746 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2438 3251 1186 Size: 1252 813 3906 Fragments arranged by size: 3906 1252 813 BseYI C'CCAG_C Cuts at: 5174 5670 5174 Size: 496 5475 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3092 1163 Size: 573 1356 4042 Fragments arranged by size: 4042 1356 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3421 4182 5210 1540 Size: 275 186 1420 761 1028 2301 Fragments arranged by size: 2301 1420 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4032 4117 5188 4032 Size: 85 1071 4815 Fragments arranged by size: 4815 1071 85 BsmI GAATG_Cn' Cuts at: 2308 3482 3575 2308 Size: 1174 93 4704 Fragments arranged by size: 4704 1174 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2534 3290 4032 4117 5188 1391 Size: 399 744 756 742 85 1071 2174 Fragments arranged by size: 2174 1071 756 744 742 399 85 BspEI T'CCGG_A Cuts at: 5634 5634 Size: 5971 BspHI T'CATG_A Cuts at: 3860 5590 3860 Size: 1730 4241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2234 2799 3064 3220 3326 844 Size: 1390 565 265 156 106 3489 Fragments arranged by size: 3489 1390 565 265 156 106 BsrBI CCG'CTC Cuts at: 1394 1823 3858 1394 Size: 429 2035 3507 Fragments arranged by size: 3507 2035 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2083 2168 4466 4640 65 Size: 1183 835 85 2298 174 1396 Fragments arranged by size: 2298 1396 1183 835 174 85 BsrFI r'CCGG_y Cuts at: 1700 1810 3155 4615 4802 1700 Size: 110 1345 1460 187 2869 Fragments arranged by size: 2869 1460 1345 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5971 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5971 BssSI C'ACGA_G Cuts at: 4031 5043 4031 Size: 1012 4959 BstAPI GCAn_nnn'nTGC Cuts at: 794 3226 794 Size: 2432 3539 BstBI TT'CG_AA Cuts at: 4775 4775 Size: 5971 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5971 BstYI r'GATC_y Cuts at: 1972 3301 3668 4053 4070 5522 5608 5620 5966 Size: 1329 367 385 17 1452 86 12 346 Cuts at: 5966 1972 Size: 1977 Fragments arranged by size: 1977 1452 1329 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2177 513 Size: 552 939 173 4307 Fragments arranged by size: 4307 939 552 173 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5232 Fragments arranged by size: 5232 683 56 BtsI GCAGTG_nn' Cuts at: 3487 4281 4301 3487 Size: 794 20 5157 Fragments arranged by size: 5157 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5936 DraI TTT'AAA Cuts at: 2737 3275 2737 Size: 538 5433 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4978 809 Size: 507 3662 1802 Fragments arranged by size: 3662 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2764 3189 3258 Size: 54 1425 50 275 18 934 425 69 Cuts at: 3258 3418 4307 5936 8 Size: 160 889 1629 43 Fragments arranged by size: 1629 1425 934 889 425 275 160 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3418 1537 Size: 275 1606 4090 Fragments arranged by size: 4090 1606 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2415 2805 3901 1353 Size: 1062 390 1096 3423 Fragments arranged by size: 3423 1096 1062 390 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4558 4932 5078 387 Size: 1064 138 2969 374 146 1280 Fragments arranged by size: 2969 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2121 2153 2426 2909 2924 2996 4034 Size: 393 489 32 273 483 15 72 1038 Cuts at: 4034 4631 5418 5686 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1038 787 597 489 483 393 273 268 72 32 15 EcoNI CCTnn'n_nnAGG Cuts at: 2103 2103 Size: 5971 EcoO109I rG'GnC_Cy Cuts at: 2029 2356 2029 Size: 327 5644 EcoRI G'AATT_C Cuts at: 3280 3658 3280 Size: 378 5593 EcoRV GAT'ATC Cuts at: 2833 2833 Size: 5971 FspI TGC'GCA Cuts at: 4477 4477 Size: 5971 HaeII r_GCGC'y Cuts at: 1678 5118 1678 Size: 3440 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2002 2034 2335 2367 2693 Size: 32 135 32 430 32 301 32 326 Cuts at: 2693 2725 4612 4644 4686 4718 1373 Size: 32 1887 32 42 32 2626 Fragments arranged by size: 2626 1887 430 326 301 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3315 3561 669 Size: 988 281 1377 246 3079 Fragments arranged by size: 3079 1377 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5971 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KpnI G_GTAC'C Cuts at: 2651 3296 2651 Size: 645 5326 MfeI C'AATT_G Cuts at: 3570 3570 Size: 5971 MluI A'CGCG_T Cuts at: 5847 5847 Size: 5971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5085 5269 1305 Size: 3780 184 2007 Fragments arranged by size: 3780 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2766 3191 10 Size: 54 1425 343 934 425 2790 Fragments arranged by size: 2790 1425 934 425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2312 3929 4288 4447 518 Size: 648 1146 1617 359 159 2042 Fragments arranged by size: 2042 1617 1146 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2479 3387 4064 5212 5457 1606 Size: 873 908 677 1148 245 2120 Fragments arranged by size: 2120 1148 908 873 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4480 Fragments arranged by size: 4480 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5971 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5971 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NruI TCG'CGA Cuts at: 2060 2060 Size: 5971 NsiI A_TGCA'T Cuts at: 2122 2122 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 3187 3329 4874 1132 Size: 97 1958 142 1545 2229 Fragments arranged by size: 2229 1958 1545 142 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5971 PciI A'CATG_T Cuts at: 4870 4870 Size: 5971 PflMI CCAn_nnn'nTGG Cuts at: 1108 2482 1108 Size: 1374 4597 PfoI T'CCnGG_A Cuts at: 2098 2098 Size: 5971 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4040 4072 5553 5585 4040 Size: 32 1481 32 4426 Fragments arranged by size: 4426 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2594 2879 3175 1613 Size: 981 285 296 4409 Fragments arranged by size: 4409 981 296 285 PsiI TTA'TAA Cuts at: 3541 3541 Size: 5971 PstI C_TGCA'G Cuts at: 830 3231 3323 830 Size: 2401 92 3478 Fragments arranged by size: 3478 2401 92 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 PvuII CAG'CTG Cuts at: 2479 2479 Size: 5971 SalI G'TCGA_C Cuts at: 1936 3313 1936 Size: 1377 4594 SapI GCTCTTCn'nnn_ Cuts at: 2805 2805 Size: 5971 SbfI CC_TGCA'GG Cuts at: 3231 3323 3231 Size: 92 5879 ScaI AGT'ACT Cuts at: 4219 5931 4219 Size: 1712 4259 SfcI C'TryA_G Cuts at: 826 1045 3227 3319 4454 5135 5326 826 Size: 219 2182 92 1135 681 191 1471 Fragments arranged by size: 2182 1471 1135 681 219 191 92 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 3155 1700 Size: 1455 4516 SmaI CCC'GGG Cuts at: 1872 3298 1872 Size: 1426 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2360 4075 4976 5238 820 Size: 197 926 417 1715 901 262 1553 Fragments arranged by size: 1715 1553 926 901 417 262 197 SnaBI TAC'GTA Cuts at: 493 2854 3762 493 Size: 2361 908 2702 Fragments arranged by size: 2702 2361 908 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 3329 1132 Size: 2197 3774 SspI AAT'ATT Cuts at: 5 52 3895 5 Size: 47 3843 2081 Fragments arranged by size: 3843 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5971 StyI C'CwwG_G Cuts at: 513 1065 2004 2517 3410 513 Size: 552 939 513 893 3074 Fragments arranged by size: 3074 939 893 552 513 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2654 2816 2909 3998 4015 4168 4353 Size: 1255 1074 162 93 1089 17 153 185 Cuts at: 4353 4798 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1089 1074 445 185 162 153 93 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4217 5929 96 Size: 274 80 33 51 1443 2240 1712 138 Fragments arranged by size: 2240 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2524 2556 5931 5963 2524 Size: 32 3375 32 2532 Fragments arranged by size: 3375 2532 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5971 XbaI T'CTAG_A Cuts at: 3307 3307 Size: 5971 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2175 2175 Size: 5971 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5971 XmaI C'CCGG_G Cuts at: 1870 3296 1870 Size: 1426 4545 XmnI GAAnn'nnTTC Cuts at: 4100 4100 Size: 5971 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3779 276 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI