Restriction map for
pFN21AB3912
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccI AccII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AspI AvaI AvrII BalI BamHI
BanII BbrPI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BglII BlnI BsaAI BsaBI BsaI
BsiEI BsiHKAI BsiWI BsmFI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
HaeIII HincII HindII HindIII HinfI
HpaI KpnI KspI MaeI MaeII
MaeIII MamI MfeI MflI MluI
MluNI MscI MspA1I MunI MvnI
NaeI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TfiI Tsp45I
TspRI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 290 TCCGGA T^CCGGA
AciI 161 GCGG CCGC(-3/-1)
AcyI 255 GGCGCC G[GA]^CG[CT]C
AfaI 406 GTAC GT^AC
AvaII 145 GGACC G^G[AT]CC
AviII 325 TGCGCA TGC^GCA
BanI 255 GGCGCC G^G[CT][GA]CC
BbeI 255 GGCGCC GGCGC^C
BbsI 17 GTCTTC GAAGAC(2/6)
BlpI 166 GCTGAGC GC^T[ACGT]AGC
BmyI 365 GTGCCC G[AGT]GC[ACT]^C
Bpu1102I 166 GCTGAGC GC^T[ACGT]AGC
BpuAI 17 GTCTTC GAAGAC(2/6)
BsaHI 255 GGCGCC G[GA]^CG[CT]C
BsaJI 137 CCACGG C^C[ACGT][ACGT]GG
BseAI 290 TCCGGA T^CCGGA
BseRI 84 CTCCTC GAGGAG(10/8)
BsgI 434 GTGCAG GTGCAG(16/14)
BsmBI 151 GAGACG CGTCTC(1/5)
Bsp1286I 365 GTGCCC G[AGT]GC[ACT]^C
BspEI 290 TCCGGA T^CCGGA
BssKI 102 CCTGG ^CC[ACGT]GG
BstNI 102 CCTGG CC^[AT]GG
CelII 166 GCTGAGC GC^T[ACGT]AGC
Cfr13I 145 GGACC G^G[ACGT]CC
DpnII 301 GATC ^GATC
DsaI 137 CCACGG C^C[GA][CT]GG
EcoNI 259 CCTCAGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 102 CCTGG ^CC[AT]GG
FauI 161 GCGGG CCCGC(4/6)
FspI 325 TGCGCA TGC^GCA
HgaI 153 GACGC GACGC(5/10)
Hin1I 255 GGCGCC G[GA]^CG[CT]C
KasI 255 GGCGCC G^GCGCC
MboI 301 GATC ^GATC
MroI 290 TCCGGA T^CCGGA
MseI 35 TTAA T^TAA
MslI 378 CACCGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 102 CCTGG CC^[AT]GG
NarI 255 GGCGCC GG^CGCC
NdeII 301 GATC ^GATC
NspI 383 ACATGC [GA]CATG^[CT]
RsaI 406 GTAC GT^AC
SapI 181 GCTCTTC GCTCTTC(1/4)
Sau3AI 301 GATC ^GATC
Sau96I 145 GGACC G^G[ACGT]CC
ScrFI 102 CCTGG CC^[ACGT]GG
SfcI 450 CTACAG C^T[GA][CT]AG
SfoI 255 GGCGCC GGC^GCC
SmlI 334 CTCAAG C^T[CT][GA]AG
Tru9I 35 TTAA T^TAA
Tsp509I 29 AATT ^AATT
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# TWO CUT ENZYMES ( 16 enzymes) #
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BbvI 41 GCTGC GCAGC(8/12)
284 GCTGC
BpmI 240 CTCCAG CTGGAG(16/14)
313 CTGGAG
BsaWI 147 ACCGGA [AT]^CCGG[AT]
290 TCCGGA
BsiYI 242 CCAGCAGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
259 CCTCAGACAGG
BslI 242 CCAGCAGTCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
259 CCTCAGACAGG
BsmAI 151 GAGAC GTCTC(1/5)
200 GAGAC
EarI 94 CTCTTC CTCTTC(1/4)
182 CTCTTC
Fnu4HI 41 GCTGC GC^[ACGT]GC
284 GCTGC
HapII 148 CCGG C^CGG
291 CCGG
HpaII 148 CCGG C^CGG
291 CCGG
ItaI 41 GCTGC GC^[ACGT]GC
284 GCTGC
Ksp632I 94 CTCTTC CTCTTC(1/4)
182 CTCTTC
MspI 148 CCGG C^CGG
291 CCGG
NlaIII 78 CATG CATG^
384 CATG
NlaIV 238 GGCTCC GG[ACGT]^[ACGT]CC
255 GGCGCC
TseI 41 GCTGC G^C[AT]GC
284 GCTGC
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# 3 OR MORE CUT ENZYMES ( 14 enzymes) #
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AluI BstF5I Cac8I CfoI DdeI
FokI HaeII HhaI HinP1I HphI
MboII MnlI MwoI SfaNI