(Circular) (Six-base) MAPSORT of: pFN21AB3797.seq Check: 9181 from: 1 to: 6127 pFN21AB3797 6127 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3937 278 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3470 1937 Size: 1533 4594 Acc65I G'GTAC_C Cuts at: 2502 3448 2502 Size: 946 5181 AclI AA'CG_TT Cuts at: 1563 3426 4254 4627 1563 Size: 1863 828 373 3063 Fragments arranged by size: 3063 1863 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2250 2799 4190 5574 5842 1632 Size: 618 549 1391 1384 268 1917 Fragments arranged by size: 1917 1391 1384 618 549 268 AflII C'TTAA_G Cuts at: 820 1017 2717 820 Size: 197 1700 4230 Fragments arranged by size: 4230 1700 197 AflIII A'CryG_T Cuts at: 1203 2922 3265 5026 6003 1203 Size: 1719 343 1761 977 1327 Fragments arranged by size: 1761 1719 1327 977 343 AgeI A'CCGG_T Cuts at: 4958 4958 Size: 6127 AhdI GACnn_n'nnGTC Cuts at: 4856 4856 Size: 6127 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6127 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6127 AlwNI CAG_nnn'CTG Cuts at: 3870 5442 3870 Size: 1572 4555 ApaI G_GGCC'C Cuts at: 3009 3009 Size: 6127 ApaLI G'TGCA_C Cuts at: 2393 4184 5340 2393 Size: 1791 1156 3180 Fragments arranged by size: 3180 1791 1156 ApoI r'AATT_y Cuts at: 1493 3436 3662 3814 5923 1493 Size: 1943 226 152 2109 1697 Fragments arranged by size: 2109 1943 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2802 4681 160 Size: 872 1770 1879 1606 Fragments arranged by size: 1879 1770 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 AvaI C'yCGr_G Cuts at: 1870 1943 3116 3452 1870 Size: 73 1173 336 4545 Fragments arranged by size: 4545 1173 336 73 BamHI G'GATC_C Cuts at: 3457 3824 3457 Size: 367 5760 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2353 2441 2502 3448 4903 Size: 325 217 627 566 88 61 946 1455 Cuts at: 4903 618 Size: 1842 Fragments arranged by size: 1842 1455 946 627 566 325 217 88 61 BanII G_rGCy'C Cuts at: 1391 3009 3446 1391 Size: 1618 437 4072 Fragments arranged by size: 4072 1618 437 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2938 928 Size: 971 1039 4117 Fragments arranged by size: 4117 1039 971 BbvCI CC'TCA_GC Cuts at: 2705 2705 Size: 6127 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2576 2610 4318 4352 1903 Size: 34 639 34 1708 34 3678 Fragments arranged by size: 3678 1708 639 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2543 4019 5229 2543 Size: 1476 1210 3441 Fragments arranged by size: 3441 1476 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6127 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4738 136 Size: 107 122 71 631 605 3066 1525 Fragments arranged by size: 3066 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6122 6122 Size: 6127 BlpI GC'TnA_GC Cuts at: 3544 3544 Size: 6127 Bme1580I G_kGCm'C Cuts at: 1790 2397 3009 4188 5344 1790 Size: 607 612 1179 1156 2573 Fragments arranged by size: 2573 1179 1156 612 607 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6127 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3371 3858 4816 Size: 416 53 83 399 24 1954 487 958 Cuts at: 4816 442 Size: 1753 Fragments arranged by size: 1954 1753 958 487 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3188 1056 Size: 2132 3995 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2296 3184 4787 1239 Size: 1057 888 1603 2579 Fragments arranged by size: 2579 1603 1057 888 Bpu10I CC'TnA_GC Cuts at: 2705 2705 Size: 6127 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4252 5117 5415 4252 Size: 865 298 4964 Fragments arranged by size: 4964 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2152 2794 3159 4790 882 Size: 617 653 642 365 1631 2219 Fragments arranged by size: 2219 1631 653 642 617 365 BsaAI yAC'GTr Cuts at: 493 1204 3918 493 Size: 711 2714 2702 Fragments arranged by size: 2714 2702 711 BsaBI GATnn'nnATC Cuts at: 2774 6121 2774 Size: 3347 2780 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3934 4316 4951 275 Size: 53 83 186 936 2401 382 635 1451 Fragments arranged by size: 2401 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4560 4958 5232 5379 5790 4560 Size: 398 274 147 411 4897 Fragments arranged by size: 4897 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2391 2421 1178 Size: 30 165 30 988 30 4884 Fragments arranged by size: 4884 988 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3307 1186 Size: 2121 4006 BseYI C'CCAG_C Cuts at: 3221 5330 5826 3221 Size: 2109 496 3522 Fragments arranged by size: 3522 2109 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2509 1163 Size: 573 773 4781 Fragments arranged by size: 4781 773 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3577 4338 5366 1540 Size: 275 186 1576 761 1028 2301 Fragments arranged by size: 2301 1576 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2397 3113 4188 4273 5344 2397 Size: 716 1075 85 1071 3180 Fragments arranged by size: 3180 1075 1071 716 85 BsmI GAATG_Cn' Cuts at: 3349 3638 3731 3349 Size: 289 93 5745 Fragments arranged by size: 5745 289 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2111 3289 2111 Size: 1178 4949 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2397 3009 3113 3446 4188 4273 5344 Size: 399 607 612 104 333 742 85 1071 Cuts at: 5344 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 612 607 399 333 104 85 BspEI T'CCGG_A Cuts at: 5790 5790 Size: 6127 BspHI T'CATG_A Cuts at: 4016 5746 4016 Size: 1730 4397 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3482 844 Size: 2638 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4014 1394 Size: 429 2191 3507 Fragments arranged by size: 3507 2191 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2205 4622 4796 65 Size: 1183 957 2417 174 1396 Fragments arranged by size: 2417 1396 1183 957 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4771 4958 1700 Size: 110 2961 187 2869 Fragments arranged by size: 2961 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6127 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6127 BssSI C'ACGA_G Cuts at: 4187 5199 4187 Size: 1012 5115 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6127 BstBI TT'CG_AA Cuts at: 4931 4931 Size: 6127 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6127 BstYI r'GATC_y Cuts at: 1972 3457 3824 4209 4226 5678 5764 5776 6122 Size: 1485 367 385 17 1452 86 12 346 Cuts at: 6122 1972 Size: 1977 Fragments arranged by size: 1977 1485 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2495 3290 2495 Size: 795 5332 BtgI C'CryG_G Cuts at: 513 1065 2181 513 Size: 552 1116 4459 Fragments arranged by size: 4459 1116 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2601 487 Size: 683 56 1375 4013 Fragments arranged by size: 4013 1375 683 56 BtsI GCAGTG_nn' Cuts at: 3643 4437 4457 3643 Size: 794 20 5313 Fragments arranged by size: 5313 794 20 ClaI AT'CG_AT Cuts at: 1554 2217 1554 Size: 663 5464 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6092 DraI TTT'AAA Cuts at: 2313 3431 2313 Size: 1118 5009 DraIII CAC_nnn'GTG Cuts at: 2401 2401 Size: 6127 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5134 809 Size: 507 3818 1802 Fragments arranged by size: 3818 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3135 3244 3574 Size: 54 1425 50 275 18 1305 109 330 Cuts at: 3574 4463 6092 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1305 889 330 275 109 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3574 1537 Size: 275 1762 4090 Fragments arranged by size: 4090 1762 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4057 1353 Size: 2704 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4714 5088 5234 387 Size: 1064 138 3125 374 146 1280 Fragments arranged by size: 3125 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2250 2296 2799 3184 4190 4787 5574 Size: 393 618 46 503 385 1006 597 787 Cuts at: 5574 5842 1239 Size: 268 1524 Fragments arranged by size: 1524 1006 787 618 597 503 393 385 268 46 EcoNI CCTnn'n_nnAGG Cuts at: 2692 2692 Size: 6127 EcoO109I rG'GnC_Cy Cuts at: 2174 3005 3006 2174 Size: 831 1 5295 Fragments arranged by size: 5295 831 1 EcoRI G'AATT_C Cuts at: 3436 3814 3436 Size: 378 5749 FspI TGC'GCA Cuts at: 2076 4633 2076 Size: 2557 3570 HaeII r_GCGC'y Cuts at: 1678 5274 1678 Size: 3596 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2369 2401 2448 2480 3344 Size: 32 135 32 797 32 47 32 864 Cuts at: 3344 3376 4768 4800 4842 4874 1373 Size: 32 1392 32 42 32 2626 Fragments arranged by size: 2626 1392 864 797 135 47 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2983 3076 3471 3717 669 Size: 988 281 1045 93 395 246 3079 Fragments arranged by size: 3079 1045 988 395 281 246 93 HindIII A'AGCT_T Cuts at: 748 2714 748 Size: 1966 4161 HpaI GTT'AAC Cuts at: 3717 3717 Size: 6127 KpnI G_GTAC'C Cuts at: 2506 3452 2506 Size: 946 5181 MfeI C'AATT_G Cuts at: 3726 3726 Size: 6127 MluI A'CGCG_T Cuts at: 6003 6003 Size: 6127 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5241 5425 1305 Size: 3936 184 2007 Fragments arranged by size: 3936 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3137 10 Size: 54 1425 343 1305 3000 Fragments arranged by size: 3000 1425 1305 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2219 2436 2921 4085 4444 4603 518 Size: 648 1053 217 485 1164 359 159 2042 Fragments arranged by size: 2042 1164 1053 648 485 359 217 159 MspA1I CmG'CkG Cuts at: 1606 2066 2477 2819 3029 3543 4220 5368 5613 Size: 460 411 342 210 514 677 1148 245 Cuts at: 5613 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 514 460 411 342 245 210 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5575 NdeI CA'TA_TG Cuts at: 387 387 Size: 6127 NheI G'CTAG_C Cuts at: 1052 3184 1052 Size: 2132 3995 NotI GC'GGCC_GC Cuts at: 3574 3574 Size: 6127 NspI r_CATG'y Cuts at: 1132 1229 2758 2926 3269 3485 5030 1132 Size: 97 1529 168 343 216 1545 2229 Fragments arranged by size: 2229 1545 1529 343 216 168 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6127 PciI A'CATG_T Cuts at: 2922 3265 5026 2922 Size: 343 1761 4023 Fragments arranged by size: 4023 1761 343 PflMI CCAn_nnn'nTGG Cuts at: 1108 2168 1108 Size: 1060 5067 PfoI T'CCnGG_A Cuts at: 2880 2880 Size: 6127 PmeI GTTT'AAAC Cuts at: 3431 3431 Size: 6127 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4196 4228 5709 5741 4196 Size: 32 1481 32 4582 Fragments arranged by size: 4582 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2174 2174 Size: 6127 PshAI GACnn'nnGTC Cuts at: 1613 2151 1613 Size: 538 5589 PsiI TTA'TAA Cuts at: 3697 3697 Size: 6127 PspOMI G'GGCC_C Cuts at: 3005 3005 Size: 6127 PstI C_TGCA'G Cuts at: 830 3479 830 Size: 2649 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6127 PvuII CAG'CTG Cuts at: 2477 2819 3029 2477 Size: 342 210 5575 Fragments arranged by size: 5575 342 210 SalI G'TCGA_C Cuts at: 1936 3469 1936 Size: 1533 4594 SbfI CC_TGCA'GG Cuts at: 3479 3479 Size: 6127 ScaI AGT'ACT Cuts at: 4375 6087 4375 Size: 1712 4415 SfcI C'TryA_G Cuts at: 826 1045 3475 4610 5291 5482 826 Size: 219 2430 1135 681 191 1471 Fragments arranged by size: 2430 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6127 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6127 SmaI CCC'GGG Cuts at: 1872 3454 1872 Size: 1582 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2717 3116 4231 5132 5394 820 Size: 197 926 774 399 1115 901 262 1553 Fragments arranged by size: 1553 1115 926 901 774 399 262 197 SnaBI TAC'GTA Cuts at: 493 3918 493 Size: 3425 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6127 SphI G_CATG'C Cuts at: 1132 3485 1132 Size: 2353 3774 SspI AAT'ATT Cuts at: 5 52 4051 5 Size: 47 3999 2081 Fragments arranged by size: 3999 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6127 StyI C'CwwG_G Cuts at: 513 1065 2529 3566 513 Size: 552 1464 1037 3074 Fragments arranged by size: 3074 1464 1037 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2341 2888 4154 4171 4324 4509 4954 Size: 1255 761 547 1266 17 153 185 445 Cuts at: 4954 325 Size: 1498 Fragments arranged by size: 1498 1266 1255 761 547 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2259 4373 6085 Size: 274 80 33 51 1443 282 2114 1712 Cuts at: 6085 96 Size: 138 Fragments arranged by size: 2114 1712 1443 282 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6087 6119 6087 Size: 32 6095 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6127 XbaI T'CTAG_A Cuts at: 3463 3463 Size: 6127 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2218 2218 Size: 6127 XhoI C'TCGA_G Cuts at: 1943 3116 1943 Size: 1173 4954 XmaI C'CCGG_G Cuts at: 1870 3452 1870 Size: 1582 4545 XmnI GAAnn'nnTTC Cuts at: 4256 4256 Size: 6127 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3935 276 Size: 53 83 186 936 2401 2468 Fragments arranged by size: 2468 2401 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI