Restriction map for
pFN21AB3797
(Linear) (Six-base) MAPSORT of: KIBB3797.seq Check: 8293 from: 1 to: 1422
KIBB3797.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
Acc65I G'GTAC_C
Cuts at: 0 497 1422
Size: 497 925
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 794 1422
Size: 245 549 628
Fragments arranged by size:
628 549 245
AflII C'TTAA_G
Cuts at: 0 712 1422
Size: 712 710
AflIII A'CryG_T
Cuts at: 0 917 1260 1422
Size: 917 343 162
Fragments arranged by size:
917 343 162
ApaI G_GGCC'C
Cuts at: 0 1004 1422
Size: 1004 418
ApaLI G'TGCA_C
Cuts at: 0 388 1422
Size: 388 1034
AseI AT'TA_AT
Cuts at: 0 797 1422
Size: 797 625
AvaI C'yCGr_G
Cuts at: 0 1111 1422
Size: 1111 311
BanI G'GyrC_C
Cuts at: 0 348 436 497 1422
Size: 348 88 61 925
Fragments arranged by size:
925 348 88 61
BanII G_rGCy'C
Cuts at: 0 1004 1422
Size: 1004 418
BbsI GAAGACnn'nnnn_
Cuts at: 0 933 1422
Size: 933 489
BbvCI CC'TCA_GC
Cuts at: 0 700 1422
Size: 700 722
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 571 605 1422
Size: 571 34 817
Fragments arranged by size:
817 571 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 538 1422
Size: 538 884
Bme1580I G_kGCm'C
Cuts at: 0 392 1004 1422
Size: 392 612 418
Fragments arranged by size:
612 418 392
BmrI ACTGGGnnnn_n'
Cuts at: 0 1366 1422
Size: 1366 56
BmtI G_CTAG'C
Cuts at: 0 1183 1422
Size: 1183 239
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 1179 1422
Size: 291 888 243
Fragments arranged by size:
888 291 243
Bpu10I CC'TnA_GC
Cuts at: 0 700 1422
Size: 700 722
BsaI GGTCTCn'nnnn_
Cuts at: 0 147 789 1154 1422
Size: 147 642 365 268
Fragments arranged by size:
642 365 268 147
BsaBI GATnn'nnATC
Cuts at: 0 769 1422
Size: 769 653
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 386 416 1422
Size: 386 30 1006
Fragments arranged by size:
1006 386 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1302 1422
Size: 1302 120
BseYI C'CCAG_C
Cuts at: 0 1216 1422
Size: 1216 206
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 504 1422
Size: 504 918
BsiHKAI G_wGCw'C
Cuts at: 0 392 1108 1422
Size: 392 716 314
Fragments arranged by size:
716 392 314
BsmI GAATG_Cn'
Cuts at: 0 1344 1422
Size: 1344 78
BsmBI CGTCTCn'nnnn_
Cuts at: 0 106 1284 1422
Size: 106 1178 138
Fragments arranged by size:
1178 138 106
Bsp1286I G_dGCh'C
Cuts at: 0 392 1004 1108 1422
Size: 392 612 104 314
Fragments arranged by size:
612 392 314 104
BsrDI GCAATG_nn'
Cuts at: 0 200 1422
Size: 200 1222
Bsu36I CC'TnA_GG
Cuts at: 0 490 1285 1422
Size: 490 795 137
Fragments arranged by size:
795 490 137
BtgI C'CryG_G
Cuts at: 0 176 1422
Size: 176 1246
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 596 1422
Size: 596 826
ClaI AT'CG_AT
Cuts at: 0 212 1422
Size: 212 1210
DraI TTT'AAA
Cuts at: 0 308 1422
Size: 308 1114
DraIII CAC_nnn'GTG
Cuts at: 0 396 1422
Size: 396 1026
EaeI y'GGCC_r
Cuts at: 0 1130 1239 1422
Size: 1130 109 183
Fragments arranged by size:
1130 183 109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 291 794 1179 1422
Size: 245 46 503 385 243
Fragments arranged by size:
503 385 245 243 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 687 1422
Size: 687 735
EcoO109I rG'GnC_Cy
Cuts at: 0 169 1000 1001 1422
Size: 169 831 1 421
Fragments arranged by size:
831 421 169 1
FspI TGC'GCA
Cuts at: 0 71 1422
Size: 71 1351
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 364 396 443 475 1339 1371 1422
Size: 364 32 47 32 864 32 51
Fragments arranged by size:
864 364 51 47 32 32 32
HincII GTy'rAC
Cuts at: 0 978 1071 1422
Size: 978 93 351
Fragments arranged by size:
978 351 93
HindIII A'AGCT_T
Cuts at: 0 709 1422
Size: 709 713
KpnI G_GTAC'C
Cuts at: 0 501 1422
Size: 501 921
MscI TGG'CCA
Cuts at: 0 1132 1422
Size: 1132 290
MslI CAynn'nnrTG
Cuts at: 0 214 431 916 1422
Size: 214 217 485 506
Fragments arranged by size:
506 485 217 214
MspA1I CmG'CkG
Cuts at: 0 61 472 814 1024 1422
Size: 61 411 342 210 398
Fragments arranged by size:
411 398 342 210 61
NheI G'CTAG_C
Cuts at: 0 1179 1422
Size: 1179 243
NspI r_CATG'y
Cuts at: 0 753 921 1264 1422
Size: 753 168 343 158
Fragments arranged by size:
753 343 168 158
PciI A'CATG_T
Cuts at: 0 917 1260 1422
Size: 917 343 162
Fragments arranged by size:
917 343 162
PflMI CCAn_nnn'nTGG
Cuts at: 0 163 1422
Size: 163 1259
PfoI T'CCnGG_A
Cuts at: 0 875 1422
Size: 875 547
PpuMI rG'GwC_Cy
Cuts at: 0 169 1422
Size: 169 1253
PshAI GACnn'nnGTC
Cuts at: 0 146 1422
Size: 146 1276
PspOMI G'GGCC_C
Cuts at: 0 1000 1422
Size: 1000 422
PvuII CAG'CTG
Cuts at: 0 472 814 1024 1422
Size: 472 342 210 398
Fragments arranged by size:
472 398 342 210
SmlI C'TyrA_G
Cuts at: 0 712 1111 1422
Size: 712 399 311
Fragments arranged by size:
712 399 311
StyI C'CwwG_G
Cuts at: 0 524 1422
Size: 524 898
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 336 883 1422
Size: 336 547 539
Fragments arranged by size:
547 539 336
TatI w'GTAC_w
Cuts at: 0 254 1422
Size: 254 1168
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 213 1422
Size: 213 1209
XhoI C'TCGA_G
Cuts at: 0 1111 1422
Size: 1111 311
Enzymes that do cut:
Acc65I AcuI AflII AflIII ApaI ApaLI AseI AvaI
BanI BanII BbsI BbvCI BcgI BciVI Bme1580I BmrI
BmtI BpmI Bpu10I BsaI BsaBI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BsrDI Bsu36I BtgI
BtgZI ClaI DraI DraIII EaeI Eco57MI EcoNI EcoO109I
FspI Hin4I HincII HindIII KpnI MscI MslI MspA1I
NheI NspI PciI PflMI PfoI PpuMI PshAI PspOMI
PvuII SmlI StyI TaqII TatI XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AgeI AhdI AleI
AlfI AloI AlwNI ApoI AscI AsiSI AvrII BaeI
BamHI BbeI BclI BfrBI BglI BglII BlpI BmgBI
BplI BpuEI BsaAI BsaHI BsaWI BsiEI BsiWI BspEI
BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I BtsI CspCI DrdI
EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI
FspAI HaeII HpaI KasI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NotI NruI NsiI PacI
PasI PmeI PmlI PpiI PsiI PspXI PsrI PstI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XmaI XmnI ZraI