(Circular) (Six-base) MAPSORT of: pFN21AB3766.seq Check: 7427 from: 1 to: 6217 pFN21AB3766 6217 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4027 278 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3560 1937 Size: 1623 4594 Acc65I G'GTAC_C Cuts at: 3538 3538 Size: 6217 AclI AA'CG_TT Cuts at: 1563 4344 4717 1563 Size: 2781 373 3063 Fragments arranged by size: 3063 2781 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2691 2850 3015 3460 4280 5664 5932 1632 Size: 1059 159 165 445 820 1384 268 1917 Fragments arranged by size: 1917 1384 1059 820 445 268 165 159 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6020 AflIII A'CryG_T Cuts at: 1203 5116 6093 1203 Size: 3913 977 1327 Fragments arranged by size: 3913 1327 977 AgeI A'CCGG_T Cuts at: 5048 5048 Size: 6217 AhdI GACnn_n'nnGTC Cuts at: 4946 4946 Size: 6217 AleI CACnn'nnGTG Cuts at: 1166 3262 1166 Size: 2096 4121 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6217 AlwNI CAG_nnn'CTG Cuts at: 3447 3960 5532 3447 Size: 513 1572 4132 Fragments arranged by size: 4132 1572 513 ApaLI G'TGCA_C Cuts at: 4274 5430 4274 Size: 1156 5061 ApoI r'AATT_y Cuts at: 1493 3503 3526 3752 3904 6013 1493 Size: 2010 23 226 152 2109 1697 Fragments arranged by size: 2109 2010 1697 226 152 23 AseI AT'TA_AT Cuts at: 160 1032 4771 160 Size: 872 3739 1606 Fragments arranged by size: 3739 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 3542 1870 Size: 73 1599 4545 Fragments arranged by size: 4545 1599 73 AvrII C'CTAG_G Cuts at: 2615 2615 Size: 6217 BamHI G'GATC_C Cuts at: 3547 3914 3547 Size: 367 5850 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3267 3538 4993 618 Size: 325 217 627 1480 271 1455 1842 Fragments arranged by size: 1842 1480 1455 627 325 271 217 BanII G_rGCy'C Cuts at: 1391 2042 2838 3536 1391 Size: 651 796 698 4072 Fragments arranged by size: 4072 796 698 651 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5246 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4408 4442 1903 Size: 34 2471 34 3678 Fragments arranged by size: 3678 2471 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4109 5319 4109 Size: 1210 5007 BclI T'GATC_A Cuts at: 1548 3121 1548 Size: 1573 4644 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2037 4828 Size: 107 122 71 631 605 337 28 2791 Cuts at: 4828 136 Size: 1525 Fragments arranged by size: 2791 1525 631 605 337 122 107 71 28 BglII A'GATC_T Cuts at: 6212 6212 Size: 6217 BlpI GC'TnA_GC Cuts at: 3634 3634 Size: 6217 Bme1580I G_kGCm'C Cuts at: 1790 4278 5434 1790 Size: 2488 1156 2573 Fragments arranged by size: 2573 2488 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6217 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3948 4906 442 Size: 416 53 83 399 24 2531 958 1753 Fragments arranged by size: 2531 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2165 1056 Size: 1109 5108 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4877 1239 Size: 3638 2579 Bpu10I CC'TnA_GC Cuts at: 3102 3481 3102 Size: 379 5838 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4342 5207 5505 4342 Size: 865 298 5054 Fragments arranged by size: 5054 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2706 4880 882 Size: 617 1207 2174 2219 Fragments arranged by size: 2219 2174 1207 617 BsaAI yAC'GTr Cuts at: 493 1204 4008 493 Size: 711 2804 2702 Fragments arranged by size: 2804 2702 711 BsaBI GATnn'nnATC Cuts at: 6211 6211 Size: 6217 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4024 4406 5041 275 Size: 53 83 186 936 2491 382 635 1451 Fragments arranged by size: 2491 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4650 5048 5322 5469 5880 4650 Size: 398 274 147 411 4987 Fragments arranged by size: 4987 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5992 Fragments arranged by size: 5992 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6217 BseYI C'CCAG_C Cuts at: 2035 5420 5916 2035 Size: 3385 496 2336 Fragments arranged by size: 3385 2336 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2256 1163 Size: 573 520 5124 Fragments arranged by size: 5124 573 520 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3667 4428 5456 1540 Size: 275 186 1666 761 1028 2301 Fragments arranged by size: 2301 1666 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2400 2838 4278 4363 5434 2400 Size: 438 1440 85 1071 3183 Fragments arranged by size: 3183 1440 1071 438 85 BsmI GAATG_Cn' Cuts at: 3031 3728 3821 3031 Size: 697 93 5427 Fragments arranged by size: 5427 697 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2042 2400 2838 3536 4278 4363 5434 Size: 399 252 358 438 698 742 85 1071 Cuts at: 5434 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 698 438 399 358 252 85 BspEI T'CCGG_A Cuts at: 5880 5880 Size: 6217 BspHI T'CATG_A Cuts at: 2886 4106 5836 2886 Size: 1220 1730 3267 Fragments arranged by size: 3267 1730 1220 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3572 844 Size: 2728 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4104 1394 Size: 429 2281 3507 Fragments arranged by size: 3507 2281 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2904 4712 4886 65 Size: 1183 1656 1808 174 1396 Fragments arranged by size: 1808 1656 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2031 4861 5048 1700 Size: 110 221 2830 187 2869 Fragments arranged by size: 2869 2830 221 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6217 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6217 BssSI C'ACGA_G Cuts at: 4277 5289 4277 Size: 1012 5205 BstAPI GCAn_nnn'nTGC Cuts at: 794 2473 794 Size: 1679 4538 BstBI TT'CG_AA Cuts at: 5021 5021 Size: 6217 BstEII G'GTnAC_C Cuts at: 1184 2201 1184 Size: 1017 5200 BstXI CCAn_nnnn'nTGG Cuts at: 2035 2035 Size: 6217 BstYI r'GATC_y Cuts at: 1972 2071 3391 3547 3914 4299 4316 5768 5854 Size: 99 1320 156 367 385 17 1452 86 Cuts at: 5854 5866 6212 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1320 385 367 346 156 99 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4726 Fragments arranged by size: 4726 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5478 Fragments arranged by size: 5478 683 56 BtsI GCAGTG_nn' Cuts at: 2316 2944 3257 3269 3733 4527 4547 2316 Size: 628 313 12 464 794 20 3986 Fragments arranged by size: 3986 794 628 464 313 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6217 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6182 DraI TTT'AAA Cuts at: 2389 3502 3521 2389 Size: 1113 19 5085 Fragments arranged by size: 5085 1113 19 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5224 809 Size: 507 3908 1802 Fragments arranged by size: 3908 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3664 4553 6182 Size: 54 1425 50 275 18 1834 889 1629 Cuts at: 6182 8 Size: 43 Fragments arranged by size: 1834 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3664 1537 Size: 275 1852 4090 Fragments arranged by size: 4090 1852 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2826 4147 1353 Size: 1473 1321 3423 Fragments arranged by size: 3423 1473 1321 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3501 4804 5178 5324 387 Size: 1064 138 1912 1303 374 146 1280 Fragments arranged by size: 1912 1303 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2836 2836 Size: 6217 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2691 2850 3015 3460 4280 4877 5664 Size: 393 1059 159 165 445 820 597 787 Cuts at: 5664 5932 1239 Size: 268 1524 Fragments arranged by size: 1524 1059 820 787 597 445 393 268 165 159 EcoRI G'AATT_C Cuts at: 3526 3904 3526 Size: 378 5839 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2291 2329 2381 2291 Size: 38 52 6127 Fragments arranged by size: 6127 52 38 FspI TGC'GCA Cuts at: 4723 4723 Size: 6217 HaeII r_GCGC'y Cuts at: 1678 5364 1678 Size: 3686 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2159 2191 4858 4890 4932 Size: 32 135 32 587 32 2667 32 42 Cuts at: 4932 4964 1373 Size: 32 2626 Fragments arranged by size: 2667 2626 587 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3561 3807 669 Size: 988 281 1623 246 3079 Fragments arranged by size: 3079 1623 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6217 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KpnI G_GTAC'C Cuts at: 3542 3542 Size: 6217 MfeI C'AATT_G Cuts at: 2300 3816 2300 Size: 1516 4701 MluI A'CGCG_T Cuts at: 6093 6093 Size: 6217 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5331 5515 1305 Size: 4026 184 2007 Fragments arranged by size: 4026 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4395 Fragments arranged by size: 4395 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3262 3513 4175 4534 4693 518 Size: 648 2096 251 662 359 159 2042 Fragments arranged by size: 2096 2042 662 648 359 251 159 MspA1I CmG'CkG Cuts at: 1606 2153 2828 3335 3633 4310 5458 5703 1606 Size: 547 675 507 298 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 675 547 507 298 245 NaeI GCC'GGC Cuts at: 2033 2033 Size: 6217 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4726 Fragments arranged by size: 4726 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6217 NgoMIV G'CCGG_C Cuts at: 2031 2031 Size: 6217 NheI G'CTAG_C Cuts at: 1052 2161 1052 Size: 1109 5108 NotI GC'GGCC_GC Cuts at: 3664 3664 Size: 6217 NspI r_CATG'y Cuts at: 1132 1229 3575 5120 1132 Size: 97 2346 1545 2229 Fragments arranged by size: 2346 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6217 PciI A'CATG_T Cuts at: 5116 5116 Size: 6217 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6217 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2712 2744 3302 3334 4286 4318 5799 5831 2712 Size: 32 558 32 952 32 1481 32 3098 Fragments arranged by size: 3098 1481 952 558 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6217 PsiI TTA'TAA Cuts at: 2450 2600 2682 3192 3787 2450 Size: 150 82 510 595 4880 Fragments arranged by size: 4880 595 510 150 82 PstI C_TGCA'G Cuts at: 830 2079 2323 3264 3569 830 Size: 1249 244 941 305 3478 Fragments arranged by size: 3478 1249 941 305 244 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 PvuII CAG'CTG Cuts at: 2153 2828 3335 2153 Size: 675 507 5035 Fragments arranged by size: 5035 675 507 SacI G_AGCT'C Cuts at: 2838 2838 Size: 6217 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SapI GCTCTTCn'nnn_ Cuts at: 2826 2826 Size: 6217 SbfI CC_TGCA'GG Cuts at: 3569 3569 Size: 6217 ScaI AGT'ACT Cuts at: 4465 6177 4465 Size: 1712 4505 SfcI C'TryA_G Cuts at: 826 1045 2075 2319 2523 3260 3565 4700 5381 Size: 219 1030 244 204 737 305 1135 681 Cuts at: 5381 5572 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1030 737 681 305 244 219 204 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6217 SmaI CCC'GGG Cuts at: 1872 3544 1872 Size: 1672 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4321 5222 5484 820 Size: 197 926 2378 901 262 1553 Fragments arranged by size: 2378 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4008 493 Size: 3515 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6217 SphI G_CATG'C Cuts at: 1132 3575 1132 Size: 2443 3774 SspI AAT'ATT Cuts at: 5 52 4141 5 Size: 47 4089 2081 Fragments arranged by size: 4089 2081 47 StuI AGG'CCT Cuts at: 1517 3050 1517 Size: 1533 4684 StyI C'CwwG_G Cuts at: 513 1065 2004 2615 3656 513 Size: 552 939 611 1041 3074 Fragments arranged by size: 3074 1041 939 611 552 SwaI ATTT'AAAT Cuts at: 3502 3502 Size: 6217 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3285 4244 4261 4414 4599 5044 325 Size: 1255 1705 959 17 153 185 445 1498 Fragments arranged by size: 1705 1498 1255 959 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2697 4463 6175 Size: 274 80 33 51 1443 720 1766 1712 Cuts at: 6175 96 Size: 138 Fragments arranged by size: 1766 1712 1443 720 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2708 2740 6177 6209 2708 Size: 32 3437 32 2716 Fragments arranged by size: 3437 2716 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6217 XbaI T'CTAG_A Cuts at: 3142 3553 3142 Size: 411 5806 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6217 XmaI C'CCGG_G Cuts at: 1870 3542 1870 Size: 1672 4545 XmnI GAAnn'nnTTC Cuts at: 3081 3317 4346 3081 Size: 236 1029 4952 Fragments arranged by size: 4952 1029 236 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4025 276 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI XcmI