Restriction map for
pFN21AB3765
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbrPI
BfrI BglII BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HgaI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MflI MluI MroI
MspA1I MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PstI PvuI PvuII RsrII
SacI SacII SalI SbfI ScaI
SexAI SfaNI SfiI SfuI SgrAI
SmaI SmlI SpeI SphI Sse8387I
SspI StuI SwaI TfiI Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AciI 1001 GCGG CCGC(-3/-1)
AclI 568 AACGTT AA^CGTT
AcyI 232 GGCGCC G[GA]^CG[CT]C
AseI 1242 ATTAAT AT^TAAT
AsnI 1242 ATTAAT AT^TAAT
Asp700I 626 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 109 GAGCAC G[AT]GC[AT]^C
BalI 132 TGGCCA TGG^CCA
BanI 232 GGCGCC G^G[CT][GA]CC
BanII 383 GAGCCC G[GA]GC[CT]^C
BbeI 232 GGCGCC GGCGC^C
BbsI 1114 GAAGAC GAAGAC(2/6)
BbvCI 1187 CCTCAGC CCTCAGC(-5/-2)
BcgI 1097 GCAGTATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1042 CTAG C^TAG
BglI 691 GCCCTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 661 CTCCAG CTGGAG(16/14)
BpuAI 1114 GAAGAC GAAGAC(2/6)
BsaAI 77 TACGTA [CT]AC^GT[GA]
BsaHI 232 GGCGCC G[GA]^CG[CT]C
BsaI 902 GAGACC GGTCTC(1/5)
BsiHKAI 109 GAGCAC G[AT]GC[AT]^C
Bsp1407I 1356 TGTACA T^GTACA
BsrDI 1292 CATTGC GCAATG(2/0)
BsrGI 1356 TGTACA T^GTACA
BssSI 1070 CACGAG CACGAG(-5/-1)
CfoI 233 GCGC GCG^C
DraI 556 TTTAAA TTT^AAA
DraII 1044 AGGGCCT [GA]G^G[ACGT]CC[CT]
EaeI 132 TGGCCA [CT]^GGCC[GA]
EarI 1301 CTCTTC CTCTTC(1/4)
EcoO109I 1044 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 129 CCTTGG C^C[AT][AT]GG
FauI 1001 GCGGG CCCGC(4/6)
HaeII 232 GGCGCC [GA]GCGC^[CT]
HhaI 233 GCGC GCG^C
Hin1I 232 GGCGCC G[GA]^CG[CT]C
HinP1I 233 GCGC G^CGC
KasI 232 GGCGCC G^GCGCC
Ksp632I 1301 CTCTTC CTCTTC(1/4)
MaeI 1042 CTAG C^TAG
MluNI 132 TGGCCA TGG^CCA
MscI 132 TGGCCA TGG^CCA
MslI 276 CATGGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 232 GGCGCC GG^CGCC
NspI 240 GCATGT [GA]CATG^[CT]
PflMI 635 CCATTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1242 ATTAAT AT^TAAT
Psp1406I 568 AACGTT AA^CGTT
SapI 1300 GCTCTTC GCTCTTC(1/4)
SfcI 1164 CTACAG C^T[GA][CT]AG
SfoI 232 GGCGCC GGC^GCC
SnaBI 77 TACGTA TAC^GTA
SspBI 1356 TGTACA T^GTACA
StyI 129 CCTTGG C^C[AT][AT]GG
TaqI 657 TCGA T^CGA
TthHB8I 657 TCGA T^CGA
Van91I 635 CCATTCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 626 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 609 GAATTT [GA]^AATT[CT]
933 AAATTC
ApoI 609 GAATTT [GA]^AATT[CT]
933 AAATTC
BciVI 925 GTATCC GTATCC(6/5)
1100 GTATCC
BclI 245 TGATCA T^GATCA
416 TGATCA
BseRI 864 CTCCTC GAGGAG(10/8)
211 GAGGAG
BsgI 14 CTGCAC GTGCAG(16/14)
388 CTGCAC
BsmFI 1031 GGGAC GGGAC(10/14)
259 GTCCC
BspHI 413 TCATGA T^CATGA
434 TCATGA
BspMI 12 ACCTGC ACCTGC(4/8)
180 GCAGGT
BstF5I 483 CATCC GGATG(2/0)
327 GGATG
Cac8I 666 GCAGGC GC[ACGT]^[ACGT]GC
1296 GCCAGC
FbaI 245 TGATCA T^GATCA
416 TGATCA
FokI 483 CATCC GGATG(9/13)
327 GGATG
MaeII 78 ACGT A^CGT
569 ACGT
MwoI 691 GCCCTCATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1291 GCATTGCCAGC
NlaIV 125 GGAGCC GG[ACGT]^[ACGT]CC
232 GGCGCC
RcaI 413 TCATGA T^CATGA
434 TCATGA
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AfaI AluI AvaII BbvI BcnI
BmyI BsaJI BsiYI BslI BsmAI
BsmI Bsp1286I BsrI BssKI BstNI
Cfr13I DdeI DpnII EcoRII Fnu4HI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MseI MspI MvaI NciI NdeII
NlaIII PleI RsaI Sau3AI Sau96I
ScrFI Tru9I TseI Tsp45I Tsp509I
TspRI