(Circular) (Six-base) MAPSORT of: pFN21AB3765.seq Check: 6349 from: 1 to: 6106 pFN21AB3765 6106 bp With 182 enzymes: SgfI * April 12, 2013 16:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2176 2176 Size: 6106 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3916 278 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3449 1937 Size: 1512 4594 Acc65I G'GTAC_C Cuts at: 3427 3427 Size: 6106 AclI AA'CG_TT Cuts at: 1563 2574 4233 4606 1563 Size: 1011 1659 373 3063 Fragments arranged by size: 3063 1659 1011 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4169 5553 5821 1632 Size: 2537 1384 268 1917 Fragments arranged by size: 2537 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5909 AflIII A'CryG_T Cuts at: 1203 5005 5982 1203 Size: 3802 977 1327 Fragments arranged by size: 3802 1327 977 AgeI A'CCGG_T Cuts at: 4937 4937 Size: 6106 AhdI GACnn_n'nnGTC Cuts at: 4835 4835 Size: 6106 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6106 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6106 AlwNI CAG_nnn'CTG Cuts at: 3849 5421 3849 Size: 1572 4534 ApaLI G'TGCA_C Cuts at: 4163 5319 4163 Size: 1156 4950 ApoI r'AATT_y Cuts at: 1493 2614 2938 3415 3641 3793 5902 1493 Size: 1121 324 477 226 152 2109 1697 Fragments arranged by size: 2109 1697 1121 477 324 226 152 AseI AT'TA_AT Cuts at: 160 1032 3248 4660 160 Size: 872 2216 1412 1606 Fragments arranged by size: 2216 1606 1412 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 AvaI C'yCGr_G Cuts at: 1870 1943 3431 1870 Size: 73 1488 4545 Fragments arranged by size: 4545 1488 73 BamHI G'GATC_C Cuts at: 3436 3803 3436 Size: 367 5739 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2237 3427 4882 618 Size: 325 217 627 450 1190 1455 1842 Fragments arranged by size: 1842 1455 1190 627 450 325 217 BanII G_rGCy'C Cuts at: 1391 2392 3425 1391 Size: 1001 1033 4072 Fragments arranged by size: 4072 1033 1001 BbeI G_GCGC'C Cuts at: 2241 2241 Size: 6106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3126 928 Size: 971 1227 3908 Fragments arranged by size: 3908 1227 971 BbvCI CC'TCA_GC Cuts at: 3193 3193 Size: 6106 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3091 3125 4297 4331 1903 Size: 34 1154 34 1172 34 3678 Fragments arranged by size: 3678 1172 1154 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2941 3116 3998 5208 2941 Size: 175 882 1210 3839 Fragments arranged by size: 3839 1210 882 175 BclI T'GATC_A Cuts at: 1548 2250 2421 1548 Size: 702 171 5233 Fragments arranged by size: 5233 702 171 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2702 4717 136 Size: 107 122 71 631 605 1030 2015 1525 Fragments arranged by size: 2015 1525 1030 631 605 122 107 71 BglII A'GATC_T Cuts at: 6101 6101 Size: 6106 BlpI GC'TnA_GC Cuts at: 3523 3523 Size: 6106 Bme1580I G_kGCm'C Cuts at: 1790 2698 4167 5323 1790 Size: 908 1469 1156 2573 Fragments arranged by size: 2573 1469 1156 908 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6106 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3837 4795 442 Size: 416 53 83 399 24 2420 958 1753 Fragments arranged by size: 2420 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6106 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2651 4766 1239 Size: 1412 2115 2579 Fragments arranged by size: 2579 2115 1412 Bpu10I CC'TnA_GC Cuts at: 2069 2145 2829 3193 2069 Size: 76 684 364 4982 Fragments arranged by size: 4982 684 364 76 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4231 5096 5394 4231 Size: 865 298 4943 Fragments arranged by size: 4943 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2901 4769 882 Size: 617 1402 1868 2219 Fragments arranged by size: 2219 1868 1402 617 BsaAI yAC'GTr Cuts at: 493 1204 2084 3897 493 Size: 711 880 1813 2702 Fragments arranged by size: 2702 1813 880 711 BsaBI GATnn'nnATC Cuts at: 6100 6100 Size: 6106 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2238 3913 4295 4930 Size: 53 83 186 936 705 1675 382 635 Cuts at: 4930 275 Size: 1451 Fragments arranged by size: 1675 1451 936 705 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4539 4937 5211 5358 5769 4539 Size: 398 274 147 411 4876 Fragments arranged by size: 4876 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2649 2679 1178 Size: 30 165 30 1246 30 4605 Fragments arranged by size: 4605 1246 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2231 2860 1186 Size: 1045 629 4432 Fragments arranged by size: 4432 1045 629 BseYI C'CCAG_C Cuts at: 5309 5805 5309 Size: 496 5610 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2004 2378 1163 Size: 573 268 374 4891 Fragments arranged by size: 4891 573 374 268 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3556 4317 5345 1540 Size: 275 186 1555 761 1028 2301 Fragments arranged by size: 2301 1555 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2118 4167 4252 5323 2118 Size: 2049 85 1071 2901 Fragments arranged by size: 2901 2049 1071 85 BsmI GAATG_Cn' Cuts at: 2047 2413 3386 3617 3710 2047 Size: 366 973 231 93 4443 Fragments arranged by size: 4443 973 366 231 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2392 2698 3425 4167 4252 5323 Size: 399 328 274 306 727 742 85 1071 Cuts at: 5323 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 727 399 328 306 274 85 BspEI T'CCGG_A Cuts at: 5769 5769 Size: 6106 BspHI T'CATG_A Cuts at: 2418 2439 3995 5725 2418 Size: 21 1556 1730 2799 Fragments arranged by size: 2799 1730 1556 21 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2026 2176 3461 844 Size: 1182 150 1285 3489 Fragments arranged by size: 3489 1285 1182 150 BsrBI CCG'CTC Cuts at: 1394 1823 3993 1394 Size: 429 2170 3507 Fragments arranged by size: 3507 2170 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3296 4601 4775 65 Size: 1183 2048 1305 174 1396 Fragments arranged by size: 2048 1396 1305 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4750 4937 1700 Size: 110 2940 187 2869 Fragments arranged by size: 2940 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3361 96 Size: 3265 2841 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6106 BssSI C'ACGA_G Cuts at: 3075 4166 5178 3075 Size: 1091 1012 4003 Fragments arranged by size: 4003 1091 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6106 BstBI TT'CG_AA Cuts at: 4910 4910 Size: 6106 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6106 BstYI r'GATC_y Cuts at: 1972 3436 3803 4188 4205 5657 5743 5755 6101 Size: 1464 367 385 17 1452 86 12 346 Cuts at: 6101 1972 Size: 1977 Fragments arranged by size: 1977 1464 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5554 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3041 487 Size: 683 56 1815 3552 Fragments arranged by size: 3552 1815 683 56 BtsI GCAGTG_nn' Cuts at: 3622 4416 4436 3622 Size: 794 20 5292 Fragments arranged by size: 5292 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6106 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6071 DraI TTT'AAA Cuts at: 2563 3410 2563 Size: 847 5259 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5113 809 Size: 507 3797 1802 Fragments arranged by size: 3797 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2137 3553 4442 Size: 54 1425 50 275 18 307 1416 889 Cuts at: 4442 6071 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1416 889 307 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3553 1537 Size: 275 1741 4090 Fragments arranged by size: 4090 1741 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3312 4036 1353 Size: 1959 724 3423 Fragments arranged by size: 3423 1959 724 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4693 5067 5213 387 Size: 1064 138 3104 374 146 1280 Fragments arranged by size: 3104 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2651 4169 4766 5553 5821 1239 Size: 393 1019 1518 597 787 268 1524 Fragments arranged by size: 1524 1518 1019 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3050 3050 Size: 6106 EcoRI G'AATT_C Cuts at: 3415 3793 3415 Size: 378 5728 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2675 2822 2675 Size: 147 5959 FspI TGC'GCA Cuts at: 4612 4612 Size: 6106 HaeII r_GCGC'y Cuts at: 1678 2241 5253 1678 Size: 563 3012 2531 Fragments arranged by size: 3012 2531 563 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2138 2170 3025 3057 3069 Size: 32 135 32 566 32 855 32 12 Cuts at: 3069 3101 4747 4779 4821 4853 1373 Size: 32 1646 32 42 32 2626 Fragments arranged by size: 2626 1646 855 566 135 42 32 32 32 32 32 32 32 12 HincII GTy'rAC Cuts at: 669 1657 1938 3450 3696 669 Size: 988 281 1512 246 3079 Fragments arranged by size: 3079 1512 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6106 HpaI GTT'AAC Cuts at: 3696 3696 Size: 6106 KasI G'GCGC_C Cuts at: 2237 2237 Size: 6106 KpnI G_GTAC'C Cuts at: 3431 3431 Size: 6106 MfeI C'AATT_G Cuts at: 3705 3705 Size: 6106 MluI A'CGCG_T Cuts at: 5982 5982 Size: 6106 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5220 5404 1305 Size: 3915 184 2007 Fragments arranged by size: 3915 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2139 10 Size: 54 1425 343 307 3977 Fragments arranged by size: 3977 1425 343 307 54 MslI CAynn'nnrTG Cuts at: 518 1166 2285 4064 4423 4582 518 Size: 648 1119 1779 359 159 2042 Fragments arranged by size: 2042 1779 1119 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3522 4199 5347 5592 1606 Size: 1916 677 1148 245 2120 Fragments arranged by size: 2120 1916 1148 677 245 NarI GG'CG_CC Cuts at: 2238 2238 Size: 6106 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5554 NdeI CA'TA_TG Cuts at: 387 387 Size: 6106 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6106 NotI GC'GGCC_GC Cuts at: 3553 3553 Size: 6106 NspI r_CATG'y Cuts at: 1132 1229 2249 3464 5009 1132 Size: 97 1020 1215 1545 2229 Fragments arranged by size: 2229 1545 1215 1020 97 PasI CC'CwG_GG Cuts at: 1348 2170 1348 Size: 822 5284 PciI A'CATG_T Cuts at: 5005 5005 Size: 6106 PflMI CCAn_nnn'nTGG Cuts at: 1108 2646 1108 Size: 1538 4568 PfoI T'CCnGG_A Cuts at: 3087 3087 Size: 6106 PmeI GTTT'AAAC Cuts at: 3410 3410 Size: 6106 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4175 4207 5688 5720 4175 Size: 32 1481 32 4561 Fragments arranged by size: 4561 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6106 PsiI TTA'TAA Cuts at: 3676 3676 Size: 6106 PstI C_TGCA'G Cuts at: 830 3458 830 Size: 2628 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6106 SalI G'TCGA_C Cuts at: 1936 3448 1936 Size: 1512 4594 SapI GCTCTTCn'nnn_ Cuts at: 3312 3312 Size: 6106 SbfI CC_TGCA'GG Cuts at: 3458 3458 Size: 6106 ScaI AGT'ACT Cuts at: 4354 6066 4354 Size: 1712 4394 SfcI C'TryA_G Cuts at: 826 1045 3169 3454 4589 5270 5461 826 Size: 219 2124 285 1135 681 191 1471 Fragments arranged by size: 2124 1471 1135 681 285 219 191 SfoI GGC'GCC Cuts at: 2239 2239 Size: 6106 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6106 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6106 SmaI CCC'GGG Cuts at: 1872 3433 1872 Size: 1561 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4210 5111 5373 820 Size: 197 926 2267 901 262 1553 Fragments arranged by size: 2267 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2084 3897 493 Size: 1591 1813 2702 Fragments arranged by size: 2702 1813 1591 SpeI A'CTAG_T Cuts at: 152 152 Size: 6106 SphI G_CATG'C Cuts at: 1132 3464 1132 Size: 2332 3774 SspI AAT'ATT Cuts at: 5 52 4030 5 Size: 47 3978 2081 Fragments arranged by size: 3978 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6106 StyI C'CwwG_G Cuts at: 513 1065 2134 3545 513 Size: 552 1069 1411 3074 Fragments arranged by size: 3074 1411 1069 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4133 4150 4303 4488 4933 325 Size: 1255 2553 17 153 185 445 1498 Fragments arranged by size: 2553 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3361 4352 6064 Size: 274 80 33 51 1443 1384 991 1712 Cuts at: 6064 96 Size: 138 Fragments arranged by size: 1712 1443 1384 991 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2605 2637 2649 2681 6066 6098 2605 Size: 32 12 32 3385 32 2613 Fragments arranged by size: 3385 2613 32 32 32 12 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6106 XbaI T'CTAG_A Cuts at: 3442 3442 Size: 6106 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6106 XmaI C'CCGG_G Cuts at: 1870 3431 1870 Size: 1561 4545 XmnI GAAnn'nnTTC Cuts at: 2635 4235 2635 Size: 1600 4506 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3914 276 Size: 53 83 186 936 2380 2468 Fragments arranged by size: 2468 2380 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI