(Circular) (Six-base) MAPSORT of: pFN21AB3761.seq Check: 7418 from: 1 to: 7909 pFN21AB3761 7909 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5719 278 Size: 53 83 186 936 4183 2468 Fragments arranged by size: 4183 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2399 5252 1937 Size: 462 2853 4594 Fragments arranged by size: 4594 2853 462 Acc65I G'GTAC_C Cuts at: 5230 5230 Size: 7909 AclI AA'CG_TT Cuts at: 1563 6036 6409 1563 Size: 4473 373 3063 Fragments arranged by size: 4473 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2751 5972 7356 7624 1632 Size: 1119 3221 1384 268 1917 Fragments arranged by size: 3221 1917 1384 1119 268 AfeI AGC'GCT Cuts at: 5005 5005 Size: 7909 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7712 AflIII A'CryG_T Cuts at: 1203 2334 3465 4469 5137 6808 7785 1203 Size: 1131 1131 1004 668 1671 977 1327 Fragments arranged by size: 1671 1327 1131 1131 1004 977 668 AgeI A'CCGG_T Cuts at: 6740 6740 Size: 7909 AhdI GACnn_n'nnGTC Cuts at: 3318 6638 3318 Size: 3320 4589 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7909 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7909 AlwNI CAG_nnn'CTG Cuts at: 2074 2086 5652 7224 2074 Size: 12 3566 1572 2759 Fragments arranged by size: 3566 2759 1572 12 ApaLI G'TGCA_C Cuts at: 5966 7122 5966 Size: 1156 6753 ApoI r'AATT_y Cuts at: 1493 2354 2386 3399 4478 4799 5218 5444 5596 Size: 861 32 1013 1079 321 419 226 152 Cuts at: 5596 7705 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1079 1013 861 419 321 226 152 32 AseI AT'TA_AT Cuts at: 160 1032 4277 6463 160 Size: 872 3245 2186 1606 Fragments arranged by size: 3245 2186 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7909 AvaI C'yCGr_G Cuts at: 1870 1943 5234 1870 Size: 73 3291 4545 Fragments arranged by size: 4545 3291 73 BamHI G'GATC_C Cuts at: 5239 5606 5239 Size: 367 7542 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5230 6685 618 Size: 325 217 627 3443 1455 1842 Fragments arranged by size: 3443 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3645 5228 1391 Size: 2254 1583 4072 Fragments arranged by size: 4072 2254 1583 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3454 3890 3985 928 Size: 971 1555 436 95 4852 Fragments arranged by size: 4852 1555 971 436 95 BbvCI CC'TCA_GC Cuts at: 2028 2028 Size: 7909 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2114 2148 5099 5133 6100 6134 1903 Size: 34 177 34 2951 34 967 34 3678 Fragments arranged by size: 3678 2951 967 177 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5801 7011 5801 Size: 1210 6699 BclI T'GATC_A Cuts at: 1548 1548 Size: 7909 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2060 6520 136 Size: 107 122 71 631 605 388 4460 1525 Fragments arranged by size: 4460 1525 631 605 388 122 107 71 BglII A'GATC_T Cuts at: 7904 7904 Size: 7909 BlpI GC'TnA_GC Cuts at: 5326 5326 Size: 7909 Bme1580I G_kGCm'C Cuts at: 1790 2654 5970 7126 1790 Size: 864 3316 1156 2573 Fragments arranged by size: 3316 2573 1156 864 BmgBI CAC'GTC Cuts at: 1318 4470 1318 Size: 3152 4757 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4124 4368 5640 Size: 416 53 83 399 24 2707 244 1272 Cuts at: 5640 6598 442 Size: 958 1753 Fragments arranged by size: 2707 1753 1272 958 416 399 244 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7909 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2885 3661 2885 Size: 776 7133 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3722 4212 6569 1239 Size: 2483 490 2357 2579 Fragments arranged by size: 2579 2483 2357 490 Bpu10I CC'TnA_GC Cuts at: 2028 4366 2028 Size: 2338 5571 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3631 6034 6899 7197 3631 Size: 2403 865 298 4343 Fragments arranged by size: 4343 2403 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6572 882 Size: 617 5073 2219 Fragments arranged by size: 5073 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4415 4636 5700 493 Size: 711 3211 221 1064 2702 Fragments arranged by size: 3211 2702 1064 711 221 BsaBI GATnn'nnATC Cuts at: 7903 7903 Size: 7909 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5716 6098 6733 275 Size: 53 83 186 936 4183 382 635 1451 Fragments arranged by size: 4183 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3119 6342 6740 7014 7161 7572 3119 Size: 3223 398 274 147 411 3456 Fragments arranged by size: 3456 3223 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3801 3831 1178 Size: 30 165 30 2398 30 5256 Fragments arranged by size: 5256 2398 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2509 3840 4523 1186 Size: 1323 1331 683 4572 Fragments arranged by size: 4572 1331 1323 683 BseYI C'CCAG_C Cuts at: 4944 7112 7608 4944 Size: 2168 496 5245 Fragments arranged by size: 5245 2168 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3705 3839 1163 Size: 573 1969 134 5233 Fragments arranged by size: 5233 1969 573 134 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2112 5359 6120 7148 1540 Size: 275 186 111 3247 761 1028 2301 Fragments arranged by size: 3247 2301 1028 761 275 186 111 BsiHKAI G_wGCw'C Cuts at: 2811 3534 5970 6055 7126 2811 Size: 723 2436 85 1071 3594 Fragments arranged by size: 3594 2436 1071 723 85 BsmI GAATG_Cn' Cuts at: 2870 5420 5513 2870 Size: 2550 93 5266 Fragments arranged by size: 5266 2550 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2654 2811 3534 3645 5228 5970 6055 Size: 399 864 157 723 111 1583 742 85 Cuts at: 6055 7126 1391 Size: 1071 2174 Fragments arranged by size: 2174 1583 1071 864 742 723 399 157 111 85 BspEI T'CCGG_A Cuts at: 7572 7572 Size: 7909 BspHI T'CATG_A Cuts at: 5798 7528 5798 Size: 1730 6179 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5264 844 Size: 4420 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3630 5796 1394 Size: 429 1807 2166 3507 Fragments arranged by size: 3507 2166 1807 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2748 6404 6578 65 Size: 1183 1500 3656 174 1396 Fragments arranged by size: 3656 1500 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3189 6553 6740 1700 Size: 110 1379 3364 187 2869 Fragments arranged by size: 3364 2869 1379 187 110 BsrGI T'GTAC_A Cuts at: 96 5013 96 Size: 4917 2992 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7909 BssSI C'ACGA_G Cuts at: 3015 5969 6981 3015 Size: 2954 1012 3943 Fragments arranged by size: 3943 2954 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3968 4187 794 Size: 3174 219 4516 Fragments arranged by size: 4516 3174 219 BstBI TT'CG_AA Cuts at: 3880 3901 6713 3880 Size: 21 2812 5076 Fragments arranged by size: 5076 2812 21 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7909 BstYI r'GATC_y Cuts at: 1972 2518 2623 2961 3356 5239 5606 5991 6008 Size: 546 105 338 395 1883 367 385 17 Cuts at: 6008 7460 7546 7558 7904 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1883 1452 546 395 385 367 346 338 105 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2223 513 Size: 552 1158 6199 Fragments arranged by size: 6199 1158 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7170 Fragments arranged by size: 7170 683 56 BtsI GCAGTG_nn' Cuts at: 2515 2913 5425 6219 6239 2515 Size: 398 2512 794 20 4185 Fragments arranged by size: 4185 2512 794 398 20 ClaI AT'CG_AT Cuts at: 1554 5109 1554 Size: 3555 4354 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7874 DraI TTT'AAA Cuts at: 2686 5213 2686 Size: 2527 5382 DraIII CAC_nnn'GTG Cuts at: 3230 3230 Size: 7909 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6916 809 Size: 507 5600 1802 Fragments arranged by size: 5600 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2109 4119 5356 Size: 54 1425 50 275 18 279 2010 1237 Cuts at: 5356 6245 7874 8 Size: 889 1629 43 Fragments arranged by size: 2010 1629 1425 1237 889 279 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2109 5356 1537 Size: 275 297 3247 4090 Fragments arranged by size: 4090 3247 297 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2727 2919 2979 3630 3898 4113 4819 5839 Size: 1374 192 60 651 268 215 706 1020 Cuts at: 5839 1353 Size: 3423 Fragments arranged by size: 3423 1374 1020 706 651 268 215 192 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6496 6870 7016 387 Size: 1064 138 4907 374 146 1280 Fragments arranged by size: 4907 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2751 3722 4212 5972 6569 7356 7624 Size: 393 1119 971 490 1760 597 787 268 Cuts at: 7624 1239 Size: 1524 Fragments arranged by size: 1760 1524 1119 971 787 597 490 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2078 2096 3960 2078 Size: 18 1864 6027 Fragments arranged by size: 6027 1864 18 EcoO109I rG'GnC_Cy Cuts at: 2071 3994 4362 2071 Size: 1923 368 5618 Fragments arranged by size: 5618 1923 368 EcoRI G'AATT_C Cuts at: 4478 5218 5596 4478 Size: 740 378 6791 Fragments arranged by size: 6791 740 378 EcoRV GAT'ATC Cuts at: 5053 5053 Size: 7909 FspI TGC'GCA Cuts at: 6415 6415 Size: 7909 HaeII r_GCGC'y Cuts at: 1678 2066 5007 7056 1678 Size: 388 2941 2049 2531 Fragments arranged by size: 2941 2531 2049 388 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3304 3336 4867 4899 5108 Size: 32 135 32 1732 32 1531 32 209 Cuts at: 5108 5140 6550 6582 6624 6656 1373 Size: 32 1410 32 42 32 2626 Fragments arranged by size: 2626 1732 1531 1410 209 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3323 5253 5499 669 Size: 988 281 1385 1930 246 3079 Fragments arranged by size: 3079 1930 1385 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7909 HpaI GTT'AAC Cuts at: 5499 5499 Size: 7909 KpnI G_GTAC'C Cuts at: 5234 5234 Size: 7909 MfeI C'AATT_G Cuts at: 5508 5508 Size: 7909 MluI A'CGCG_T Cuts at: 5137 7785 5137 Size: 2648 5261 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2209 2516 7023 7207 1305 Size: 904 307 4507 184 2007 Fragments arranged by size: 4507 2007 904 307 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4121 10 Size: 54 1425 343 2289 3798 Fragments arranged by size: 3798 2289 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4977 5867 6226 6385 518 Size: 648 3811 890 359 159 2042 Fragments arranged by size: 3811 2042 890 648 359 159 MspA1I CmG'CkG Cuts at: 1606 5325 6002 7150 7395 1606 Size: 3719 677 1148 245 2120 Fragments arranged by size: 3719 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7357 NdeI CA'TA_TG Cuts at: 387 2455 4872 387 Size: 2068 2417 3424 Fragments arranged by size: 3424 2417 2068 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7909 NotI GC'GGCC_GC Cuts at: 5356 5356 Size: 7909 NspI r_CATG'y Cuts at: 1132 1229 2187 2205 2338 3469 5267 6812 1132 Size: 97 958 18 133 1131 1798 1545 2229 Fragments arranged by size: 2229 1798 1545 1131 958 133 97 18 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7909 PciI A'CATG_T Cuts at: 2334 3465 6808 2334 Size: 1131 3343 3435 Fragments arranged by size: 3435 3343 1131 PflMI CCAn_nnn'nTGG Cuts at: 1108 4172 4876 1108 Size: 3064 704 4141 Fragments arranged by size: 4141 3064 704 PfoI T'CCnGG_A Cuts at: 2871 2871 Size: 7909 PmeI GTTT'AAAC Cuts at: 5213 5213 Size: 7909 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5978 6010 7491 7523 5978 Size: 32 1481 32 6364 Fragments arranged by size: 6364 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3994 4362 3994 Size: 368 7541 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7909 PsiI TTA'TAA Cuts at: 3242 3411 3606 4796 5479 3242 Size: 169 195 1190 683 5672 Fragments arranged by size: 5672 1190 683 195 169 PstI C_TGCA'G Cuts at: 830 5261 830 Size: 4431 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7909 SalI G'TCGA_C Cuts at: 1936 5251 1936 Size: 3315 4594 SapI GCTCTTCn'nnn_ Cuts at: 2727 2727 Size: 7909 SbfI CC_TGCA'GG Cuts at: 5261 5261 Size: 7909 ScaI AGT'ACT Cuts at: 6157 7869 6157 Size: 1712 6197 SexAI A'CCwGG_T Cuts at: 4096 4096 Size: 7909 SfcI C'TryA_G Cuts at: 826 1045 2066 2149 3541 3946 5177 5257 6392 Size: 219 1021 83 1392 405 1231 80 1135 Cuts at: 6392 7073 7264 826 Size: 681 191 1471 Fragments arranged by size: 1471 1392 1231 1135 1021 681 405 219 191 83 80 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7909 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7909 SmaI CCC'GGG Cuts at: 1872 5236 1872 Size: 3364 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3646 6013 6914 7176 820 Size: 197 926 1703 2367 901 262 1553 Fragments arranged by size: 2367 1703 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5700 493 Size: 5207 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7909 SphI G_CATG'C Cuts at: 1132 2205 5267 1132 Size: 1073 3062 3774 Fragments arranged by size: 3774 3062 1073 SspI AAT'ATT Cuts at: 5 52 2409 3247 5833 5 Size: 47 2357 838 2586 2081 Fragments arranged by size: 2586 2357 2081 838 47 StuI AGG'CCT Cuts at: 1517 4989 1517 Size: 3472 4437 StyI C'CwwG_G Cuts at: 513 1065 3563 5348 513 Size: 552 2498 1785 3074 Fragments arranged by size: 3074 2498 1785 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3191 3876 5936 5953 6106 6291 6736 Size: 1255 1611 685 2060 17 153 185 445 Cuts at: 6736 325 Size: 1498 Fragments arranged by size: 2060 1611 1498 1255 685 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2755 5013 6155 Size: 274 80 33 51 1443 778 2258 1142 Cuts at: 6155 7867 96 Size: 1712 138 Fragments arranged by size: 2258 1712 1443 1142 778 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3801 3833 7869 7901 3801 Size: 32 4036 32 3809 Fragments arranged by size: 4036 3809 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7909 XbaI T'CTAG_A Cuts at: 5245 5245 Size: 7909 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7909 XmaI C'CCGG_G Cuts at: 1870 5234 1870 Size: 3364 4545 XmnI GAAnn'nnTTC Cuts at: 6038 6038 Size: 7909 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5717 276 Size: 53 83 186 936 4183 2468 Fragments arranged by size: 4183 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI